Jerk with a Heart of Gold: Natsuno is aloof to most of the villagers, but cares deeply about Tohru, Akira, and Kaori. He tries to send Akira and Kaori away, but they end up coming back to help him. After finding out that Tohru has become a Shiki, he lets him feed on him without much of a fight and even suggests trying to run away together. He later ends up saving Akira from a Shiki and helping both him and Kaori escape from their village before the final confrontation.
Not so Above It All: Although he's one of the first people to really start investigating the vampires, once he learns that his best friend Tohru has become one he immediately loses all will to fight and suggests that Toru feed on him instead—until he dies! Sure, he comes back as a werewolf, but he had no way of knowing that would happen at the time.
The director of the clinic in Sotoba. He is baffled by the mysterious deaths and swears to solve the case and protect his village. He is one of the first people to discover the existence of the Shiki, and ends up taking pretty extreme measures against them.
Agent Mulder: Oddly enough, the village's man of science is one of the first to think the epidemic may be supernatural.
Batman Gambit: He outs the Shiki by pretending to surrender to them and tricking Chizuru into going on a date with him to get her to the Buddhist shrine so she can Freak Out! and get a bunch of people to escort her away, allowing them to feel for themselves her lack of a pulse and body temperature.
Former Teen Rebel: Was one as a child and inspired Seishin into still hating the village later on in his life when he was on the verge of giving up. He also married his wife out of spite for his parents who hated her.
He Who Fights Monsters: As he slowly loses more and more villagers to the plague, his psyche and determination begin to deteriorate, resulting in his appearance becoming haggard and strained. Eventually he resorts to experimenting on his wife when she rises as a Shiki in an attempt to either cure the plague and, failing that, to find the most efficient means of killing them.
Heel Realization: Through his conversations with Sunako, he comes to the conclusion that he and the Shiki are Not So Different and that he has more in common with them than he does with the other villagers.
A village girl who hopes to one day leave Sotoba and create a career for herself in the city. She has an unrequited crush on Natsuno, who finds her annoying and cannot stand her. Megumi is one of the first victims of the Shiki and is also the first to rise.
Expy: Of Yuno Gasai from Future Diary. Stalker with a Crush? Check. Hates anyone who gets close to her Yuuki? Check. Becomes a Yandere? Check. Dreams of having a better life, and attaches her dreams to someone else? Check. Has pink hair and eyes? Check.
For the Evulz: She often mocks those who refuse to kill like Masao and Tohru, and in Episode 13, she can be seen skipping around with a smile on her face saying how making someone she hates makes her feel good.
Irony: In her human life, she was always complaining about living in the small village and wanting to go out and live in a big city. Then she dies and turns into a Shiki, gaining the freedom she long sought for, but can't leave because the Kirishiki won't allow her to be part of the Big City Unit which captures people to feed the Shiki in the village, so she takes out her frustrations on the villagers instead. This winds up backfiring big time when the villagers strike back, and by the time things have gotten so bad for the Shiki, only then does she decide to flee to the city, only to barely make it to the city limits before she is caught, staked, and put down for good, dying in the small village she always hated.
Laser-Guided Karma: Yes she has some moments of kindness after becoming a Shiki but they're few and fair. When it comes down to it, she willingly antagonized the village for her her amusement and selfishness. Most of the others only did it to survive or were strong-armed into it. She has no such excuse and it was because of Shiki like her that the villagers came down so hard on the Shiki as a result.
Pet the Dog: Megumi is arguably one of the absolute least sympathetic characters in the series, but even she gets one of these when she first begs Tatsumi not to kill Natsuno and then offers to kill him so Tohru won't have to.
"Shut up you creep! How can scum like you rise up when Yuuki was cremated?! He deserve to be here instead of you! Why don't you take his place and die?! Just shut up. Yuuki should've risen and become one of us, he would've climbed the ranks right away-stright to the top! He wouldn't be a bottom feeder like you."
And even after this, he still questioned Megumi's love for Yuuki, earning him a good kick to the face.
Violently Protective Girlfriend: Although given how she and Natsuno never became an Official Couple, she is clearly one for him. Going as far as to yell at anyone who dared show any harm to Yuuki. She was willing even to disobey orders, regardless of what may happen to her, just to protect Yuuki.
Yandere: Becomes one after rising as a Shiki, killing Tohru because, as his best friend, Natsuno likes him more than her. Not to mention how she starts to mess with Kaori's life just because she and Natsuno became friends.
A friend of Megumi and later Natsuno. She and her brother Akira are some of the first characters to make connections between the recent deaths and the mysterious new family. She is kind-hearted, but easily scared.
Break the Cutie: First her best friend dies out of the blue... later her new friend, with whom she found out the truth behind the recent deaths in her village, dies after she and her brother try to save him. Then her father dies and she is informed of it by her ex-best friend, who is in fact her father's killer. Her mother also ends up dying and later her brother disappears, leaving her thinking that he is also dead. Poor Kaori goes Ax-Crazy when her undead father tries to kill her. She applies a baseball bat to his head multiple times, and when that doesn't kill him, she gets out a pickaxe to finish him off. She then proceeds to shove his unrecognizable corpse into a closet for good measure.
Despair Event Horizon: She goes through one after her parents die, Akira disappears and she presumes he is also dead... and then she has to kill her father again. While she and Akira reunite at the end, she never seems to really get much better.
An obnoxious high school second year. Prone to whining and moaning about his lot in life despite others being much worse off. Another Shiki victim who rises.
Ain't Too Proud to Beg: In the anime, he's so terrified at the prospect of dying that he begs for help from his sister-in-law, though of course she kills him instead. It's arguably the most sympathetic he's been all series.
Harmless Villain: Despite all his bad qualities, he apparently couldn't bring himself to kill innocent humans, which Megumi bitches him out for. Subverted in the manga, where he was more than willing to turn his family members into his slaves starting with his niece in order to survive, only to get a knife stabbed into his head and a speech that describes the kind of person he is from his sister-in-law before she kills him.
The Unfavorite: He seems to be treated like this by his family, to the point they let him die all alone while they look after his nephew and then later seem to have completely forgotten that he was one of the Shiki's casualties to avenge. Although he does more-or-less seem to bring it on himself, and at least in the manga it's revealed that he used to be the favorite before his nephew came along.
What Does She See in Him?: He honestly can't understand why girls like Megumi would ever fall in love with cold cool guys like Yuuki.
Heel Realization: In the manga at least, when facing death at the hands of the villagers, she realizes that she is no different from the serial killers and murderers she once denounced.
Irony: She escapes from her Abusive Parents and finally finds a loving family in Sotoba, only to have it all taken away from her when none of the others rise when she kills them. And she knows that it's her own fault, too.
Transhuman Treachery: Whilst most of the Shiki are guilty of this, Nao is a particularly good example. She goes after her family before anyone else, hoping that they will rise too so that she won't be alone anymore.
Karmic Death: He kills Seishin's family when the monk joins up with the Shiki. Muroi returns the favor.
Knight Templar: In the anime, he kills a human who was under Shiki hypnosis, believing that he was a Shiki collaborator. He is also part of the group that killed Seishin's family after it became clear he was helping Sunako. Subverted in the manga at first, where he doesn't harm the hypnotized humans even after being stabbed by them, but then double subverted with his slaughter of the people at the temple in the manga.
The Unfettered: Ookawa is utterly devoted to wiping out the Shiki when they get outed in the anime, taking control of the mob from Toshio and showing little remorse for the often innocent people he kills in his goal.
A mysterious family who move into the Kanemasa mansion at the start of the series. They are the original Shiki group.
Interrupted Suicide: Decides to stay and die in the fire when her plan and the village burn around her. Seishin has a different idea.
It's All About Me: A more subtle take on the trope than usual. She is genuinely sad that she had killed many people, but she spends the majority of her mourning time finding excuses for herself and her actions, and never even tries to minimize the deaths. She's the one who planned to slaughter an entire village, and to kidnap random innocent people from the next city, just so she could have a village of her pawn to keep her company.
Japanese Christian: Makes several references to the Bible, especially Cain and Abel; and when things get bad her first impulse is to go to an abandoned church and ask God for forgiveness and protection.
Karma Houdini: Despite all the people who die because of her, she gets away scot-free. Except she loses everyone she ever cared about save Seishin by the end, so she likely wouldn't be particularly happy with the outcome of her escape.
Really 700 Years Old: Despite her appearance of a 13-year-old girl, she's at least two centuries old, and the oldest of the Shiki in Sotoba, being a mother figure to Chizuru rather than the other way around.
The mother of Sunako and wife of Seishirou. Actually a lie, she's another Shiki and actually younger than Sunako. When she was alive, Chizuru was a loving wife until her husband died during war, and she was later turned into a Shiki and wondered around lost without love... until she found Seishirou.
Alas, Poor Villain: Chizuru was a total bitch and ruthless killer, but it's hard not to feel sorry for her when she is getting lynched while begging for her life when she was discovered as a Shiki by the villagers.
Hidden Depths: When we first see him, he appears to be friendly and harmless. Of course this is all a lie, but it's only around Episode 7 that he shows his true colors as a cold, cruel master manipulator. And it's even later when we learn about just how deep his devotion is to Sunako. These three sides of his personality make him a more well-rounded character than he otherwise would be if he only had one or two.
Rasputinian Death: The guy gets shot and driven over multiple times before falling into a fissure. What finally kills him differs between the manga (staked on a stalagmite) and anime (blown up by dynamite).
Straw Nihilist: Reveals in his final moments that he is a nihilist in the manga. Knowing that Sunako's actions were futile, he just wanted to see the Shiki and mankind wipe each other out.