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troperville

In the Lord of the Rings fanfic Ancient Prophecy fullfilled, one of the Valar (the gods without Capital G) slew the children of their Guardians. Understandably the Guardians in question weren't so thrilled. They battled the Fourteen Valar, destroyed the city they dwelled in and still inspire such fear that the Valar have to call upon the in that fanfic former Big Bad to aid them!
Karofsky's mom in the Glee fanfic The Kurtofsky I Ms. There's a reason she demands to be called Mrs. Badass and the reason is that she beat up Azimio with a golf club when he tried to destroy Karofsky's car.
Twilight Sparkle. Do not hurt Nyx, physically or psychologically, or she will demonstrate exactly why she is Celestia's protege. She'll even go so far as to attack Celestia herself if she thinks it necessary, though there are limits to even the Mama Bear power-up so fortunately nothing much comes of it.
In Families, Twilight rips a tree out of the ground and uses it as a club against some teenage dragons threatening Spike! In the next chapter, Garble (the teen dragons' leader) makes Twilight so furious that she goes into Rapidash-mode and shoves a burning piece of wood through his eye!
- This line says it all:
Twilight Sparkle: Get. Away. From. My. Son!
Mary Jane Watson is an unusual variant in that she's a Daughter Bear. She currently has much better control over her emotions than she did when she first became a superheroine, but threatening her family and close friends is still her Berserk Button and anyone who threatens them will suffer.
In the Pony POV Series, Ditzy Doo (better known as Derpy Hooves) grabs a mop and proceeds to beat the living tar out of Princess Gaia's birds who have come to carry her daughter away. She actually scares the crap out of the Big Bad doing this!
- The Dark World version of her also qualifies. She kept going for a thousand years of none stop pain and suffering to keep her end of Discord's bargain to turn Dinky back (Discord turned her into a muffin). When the redeemed Twilight, Applejack, and Rarity rescue her along with Spike, she joins them to reunite the Elements so she can uncurse Dinky, being revealed as an awakened Element of Loyalty due to her loyalty to her daughter.
- Rarity goes Mama Bear when her baby sister Sweetie Belle is threatened. She even fights Princess Gaia's Dragon, Cheerilee, one-on-one and her zeal is enough to put them on equal hooves. Her rage was great enough that she almost killed Cheerilee if the others hadn't reminded her that Cheerilee was Brainwashed and Crazy at the time and they had to be careful.
- G1 Mimic's Dying Moment of Awesome is one of the most popular moments in the entire fic, due to the fact she managed to bust out Discord's tooth, becoming the only mortal pony to ever truly hurt him. We find out in "Origins" that she did it to protect Celestia and Luna, who were her daughters. It didn't help Discord essentually just emotionally curbstompped Celestia For the Evulz. The moment is preceeded by this Badass Boast:
Mimic: You float one inch towards my daughters I will kill you and seal your spirit in one of my horseshoes!
- Exploited by Discord with his earthly mother Shady. He knows she loves him, and thus powers her up with an Element of Chaos, allowing her to be his loyal Dragon and bodyguard, safe in the knowledge she loves him too much to let anything happen to him. Unfortunately for him, he didn't realize she might love him enough to realize he'd gone too far and had to be stopped.
In Harmony In Disarray Alex Russo is this towards Leigh as she once broke Galloway's nose (and her own hand!) when he pushed Leigh, and before that she was already one as far back as the beginning of the story.
Every female character with a child in The Legend of Spyro: A New Dawn and it's sequels, even the Big Bad Deadlock. Given most of them are female Dragons, it seems it's just a natural trait for them to have. Cynder takes it up a notch, due to her own horrific childhood making her want to protect her child from suffering the same thing. The only subversion is Queen Gorgon, the Queen of the Naga that Deadlock overthrew.
Ditzy Doo gets another dose of this in the My Little Pony: Friendship Is Magic fanfic Shipping and Handling, although since it's a shipping/comedy fic rather than an adventure story, both the trigger and the flipout are relatively tame (but we dread to think what would happen if her daughter Dinky was seriously threatened). She relatively meekly endures a verbal beatdown by the unicorn Glow, who's kind of a bitch, until Glow insults Dinky. At this point, Ditzy a) goes berserk, b) throws a serving pan at Glow's head, and c) tells her where to stick it.
You can insult my eyes, my intelligence, and my disposition all you want, but I will NOT tolerate unkind words about my daughter! Now take it back, you overbearing, self-centered mule!
- Derpy has another prominent moment of this is The Party Never Ended, where she uses thoughts about Dinky to motivate herself to help fend off the storm.
- And another one in Ace Combat The Equestrian War. When the griffins raid Ponyville in chapter 4 to kidnap fillies and Dinky is taken, Derpy doesn't hesitate to fight three griffins all by herself.
In the Axis Powers Hetalia fanfiction The Howl of the Wind, the spirit of the personification of Native America returns to torment the various nation for raping America. While haunting France in his sleep, she actually turns herself into a bear and starts clawing him to pieces.
Princess Celestia in Progress and spin-offs will ever so calmly, politely, and nightmarishly deal with you if you threaten her sister Luna.
In Broken Bow, do not, ever, EVER, attempt to harm, marry or hide Armani Dove within the vicinity of Artemis, if you don't want to be attacked with so much force, that Athena thinks could even destroy Zeus. It doesn't matter if your Aphrodite, a clueless nymph, or even her own Brother....just don't
Chronomistress Out Of Time: Derpy who, upon thinking that her daughter might be in danger, gets out of a Lotus-Eater Machine with sheer force of will, then proceeds to take on an entire hive of changelings.
Imperfect Metamorphosis: Kotohime, though typically maintaining a veneer of amicable dutifulness, has no quarter towards anyone who harms officers under her command, even if that individual happens to be Marisa Kirisame.
- In turn, Marisa's mother figure, Mima, is terribly protective of her. Marisa tends to get uncomfortable when Mima voices her concerns regarding the witch's mortality, and how to solve that little "problem". Of course, this doesn't stop Marisa from using it to coerce Mima into Yukari's god-killing hit squad.