Animal Themed Superbeing: Based on lab rats, spefically on the notion of their use in experiments.
Anti-Hero/Anti-Villain: He is classified as a supervillain because he was forced to rob the Kizaki mansion for his debut as Ratman and because he beat the stuffing out of ANKaiser, but he's not evil.
Broken Faceplate: When Ratman gets excited or angry his faceplate splits into a mouth full of fangs.
Clueless Chick Magnet: Mirea and Rio have both expressed interest in him, but he has no idea, much to the former's annoyance. Shiki continually lampshades this.
Dark Is Not Evil: As Ratman he is around six feet tall, dressed in what appears to be a black bodysuit, Thigh-High Boots, and a Scarf of Asskicking, and has a faceplate that morphs into a mouthful of fangs. He's also nicer than a lot of the superheroes.
Eyeless Face: As Ratman his "helmet" has no eyes, just red markings.
Good Scars, Evil Scars: Has a scar on his back from [[spoiler; being crushed by a chunk of concrete]] when he was younger.
Minion with an F in Evil: Despite working for a "villainous" organization, Shuto acts more like The Cowl in his spare time, helping Rio solve crimes and hanging out with Fatman. Crea calls him out on it.
Oblivious to Love: Doesn't seem to realize the feelings that Mirea has towards him.
Otaku: Shuto constantly nerds out over anime and heroes, and is often picked on for it.
One-Winged Angel: When Shuto thinks IS-KA has killed Mirea his S-Genes kick into overdrive and he becomes a mindless monster that makes his previous berserk form seem tame.
Power Incontinence: Has difficulty controlling Ratman's transformations.
Unskilled, but Strong: After getting beaten up by a bully, he comes to the realization that he's mainly won because of his Ratman powers and decides to train himself
Sempai of Shuto and Mirea. Daughter of the president of the Heroes' Association who dreams of being a hero herself someday to help people.
Action Girl: Is introduced saving Shuto from being beaten up by a group of delinquents, and spends her downtime training in martial arts and investigating crimes.
An evil organization who opposes the Heroes' Association.
Card Carrying Villains: Crea has stated their goal is world domination, but she was lying.
Enemy Mine: With the Hero Organization against IS-KA.
Evil Is One Big Happy Family: Literally. Aside from the Jackie combatants and Shuto, Jackal is solely comprised of the surviving members of the Mizushima family.
Organization With A Dark Secret: Crea and Mirea's parents were two of the founding members of the Hero Organization and were involved in the creation of Shiningman.
Revenge: Chapter nineteen confirms this as their true goal.
Villains Out Shopping: The Jackie combatants are frequently dispatched to purchase food supplies, often with humorous results. Reality Ensues when Mirea is photographed with them by a reporter for the chief hero magazine and Ratman has to rush to prevent it from being published.
Crea Mizushima
The leader of Jackal who made the technology of Ratman's APPEND GEAR. Mirea's older sister.
Bare Your Midriff: Is fond of wearing somewhat-revealing clothes under her lab coat.
Not-So-Harmless Villain: Despite their goofy antics and nonthreatening appearance, they have displayed some skill at hand-to-hand combat, good reflexes, and an affinity for appearing out of nowhere.
The Mole: Joined up to investigate the S-Genes. Also a rare positive variant, as the people he's working with have made it clear that they are not above breaking the law and doing downright awful things. At least Jackal avoids the latter...
Reverse Grip: How he occasionally holds his weapons.
Brought Down to Badass: Ratman's Shining Ray shatters his Unchain exoskeleton and he doesn't use it again until the fight with IS-KA, but he retains his psychic powers.
Cannibalism Superpower: Can assimilate the genes of predatory animals he devours, an ability he calls "Swallow".
Evil Counterpart: A shady organization's guinea pig and personal muscle? Check. Evil Is One Big Happy Family setting and genuinely supportive and caring coworkers? NOPE. Poor thing...
Evil Knockoff: As Unchain he looks suspiciously similar to Ratman.
Karma Houdini: Played with. He's depicted as a legitimate victim and clearly doesn't enjoy having to be evil when in control of himself. Rio realizes this and essentially forgets about how he was one of her captors.
Laughing Mad: When he finds out Shuto is Ratman he telekinetically tears the school roof apart while cackling.
A hero who worked for the security company ANK Security. After the company flopped, he retired from being a hero but became one again when his gym hired him to be their commercial hero and renamed itself Kaiser Boxing Gym.
Superheroes Wear Capes: Wears one for a while until he gets rid of the cape when he gets a new hero suit in Episode 25.
Mikoto Kujo/Ame-No-Uzume-No-Mikotonote Better known as Mikoto or Heavenly Maiden
A heroic swordswoman who also happens to be a first-year student at Shuto's school. The youngest A-ranked hero, and the commercial hero and the sixteenth owner of the vintage fiber manufacturing company Haori.
A-Cup Angst: Expresses embarrassment when IS-KA lists her three sizes.
Action Girl: One of the youngest superheroes registered at the Association, but holds an A-rank.
Bare Your Midriff: Her uniform exposes much of the front of her body.
Blue Blood: The motif and themes of her hero-self shows elements of this. The company that sponsors her is apparently a very elite, highly respected company that has been trading and making high-quality fabrics and gowns for several centuries. Think a designer brand that mostly caters to the very elite, rather than just about anyone who's willing to shell out the cash. Not to mention her title.
Hair Decorations: Keeps her hair tied up in a ponytail with a white ribbon in her civilian identity, and in her superhero outfit her armour gives her looped pigtails.
Luminescent Blush: Has this reaction to having her three sizes listed and the fact that her panties are showing pointed out.
Scarf of Asskicking: As Ame-No-Uzume-No-Mikoto she wears one that invokes female kami. It is also a literal scarf of asskicking, as she can direct it to protect her or attack.
Sempai Kohai: Is Shuto's kohai, and calls him her sempai.
This Is a Drill: Can wrap her scarf around her sword to transform it into a drill, increasing its offensive power.
The Quiet One: Rarely talks except when transforming.
IS-KA
An newly-registered but powerful hero working for the Ad-Bank cellphone company. He is connected to Ratman through the S-Genes, and aims to show the world how monstrous heroes can be by infecting them with S-Genes.
Hero with an F in Good: His introduction has him plotting to attack Shuto's school and throwing a fireball at Mikoto. It turns out he's not a hero at all.
Took a Level in Badass: Actually handles the berserk Mr. Big Horn better in battle then Ratman, takes him on solo and wins when he thinks Ratman is going to activate a similar power.
Warrior Therapist: Guesses without being told that Shuto was going to try to tap into the same S-Genes that were driving other heroes crazy and manages to get to the root of Mr. Big Horn's issues that drove him to use the hero amplifier, momentarily talking him out of his rampage.
Exclusive hero of fast food chain Bison Burger who moved from the United States to Japan. He was initially a D-Lister before upgrading to the C-List by downloading the Hero Amplifier app.
Hair Decorations: Ana wears a black ribbon with her hair bun on the left side of her head, while Yuna wears a white ribbon with her hair bun on the right side of her head.