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"Luigi, I'm Home!" Wait, wrong skit...
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    Animated Segments 
  • "The Bird! The Bird!":
    • The scene where King Koopa orders his minions to lick his feet, which comes off as either bizarre or funny, but the super-fast rendition of the SMB2 Ground theme playing while they do so definitely makes it funny.
    • Mario lamenting how freezing cold it is on the mountaintop.
      Mario: I'd pay 200 bucks for a nice pepperoni cheesecake right now...
      Cheepy: (cheeps from within ice)
      Mario: Who call me cheap?! I pay 300 bucks!
  • In "Butch Mario and the Luigi Kid", after Toad explodes the wall of the prison to free the Mario Bros., we get this:
    Luigi: I need a doctor!
    Mario: I need a pizza!
  • "The Great Gladiator Gig" has plenty of funny conversations:
    • Koopa: Guess who's coming to dinner, Tryclydius — the Marios!
      Tryclyde: Mash 'em! Stump 'em! Crush 'em!
      Koopa: Don't let 'em see you, snake-breath. I want 'em to walk into my trap.
      Tryclyde: Mash 'em! Stump 'em! Crush 'em!
      Koopa: Why do I have to give you an order three times before it sinks in?!
      Tryclyde heads: I don't know, ask him. / I don't know, ask him.
    • "Uh, excuse my brother, he gets nervous around guys six times bigger than him!"
    • Mario: This is some place, hey Luigi?
      Luigi: It's some place, but I don't know what place.
      Brutius: This is the place where we capture you!
      Mario: Oh. See, Luigi? This is the place where they—
      Mario and Luigi: Capture us!?
    • "Wait a macaroni minute! I got an idea! Dance, Luigi!" "You lost your noodle!?"
  • At the end of "The Great BMX Race", Princess Toadstool and Toad inform Mario and Luigi that the race they just won (by default) was a big set-up by Koopa. The brothers are furious upon hearing this (Luigi because he risked his neck for nothing and Mario because he wasted his can of tomato sauce) and the moment Koopa appears to gloat, the Mario Bros, along with Princess Toadstool and Toad, chase after him throwing vegatables.
  • "Koopenstein": When Mario's plan to get into Koopenstein's tower fails, Toad complains and suggest that he climb the wall of the tower and let everyone in. Later, Toad is regretting this while climbing up the wall.
    Toad: Me and my big mouth! Why didn't I just suggest we go back to the village and think about it?
  • "Princess, I Shrunk the Mario Brothers":
    Koopa: Know what I'm gonna do with you buttinskis?
    Mario: Say you're sorry and let us go?
  • "Mario Meets Koopzilla"
    • "Oh, not again! I just had that ceiling fixed!"
    • While Mario and Koopzilla fight, Luigi, Princess and Toad run behind a building with a huge needle ready to shrink Koopzilla.
    Toad: This is the second biggest hypodermic needle I've ever seen!
    Luigi: What was the biggest?
    Toad: My last flu shot!
  • "King Mario of Cramalot":
    Koopa: So, Mario thinks he's king of Cramalot. That impudent little faucet-fixer! How dare he! There's only one king of Cramalot! And who is that?
    Koopa Troopa: Ahh, can ya give me a hint?
    Koopa: He's sitting right here on this throne!
    Koopa Troopa: Well gee, maybe ya better get off him.
    (Brief pause as Koopa glances at the viewer. He then grabs the Koopa Troopa by the throat to holler...)
    Koopa: I'm talking about me, you know-nothing nincom-Koop! (clouts the Koopa Troopa over the head with his sceptre)
    • Then there's the scene of the heroes trying to get into Cramalot.
      Mario: Luigi, you have to swim across the moat and lower the drawbridge.
      Luigi: ME? B-b-but, I can't! I'm gonna have a baby! (pretends to moan in pain)
      • Mario's face after that is priceless.
    • After Mario and his friends get captured and put into a dark room, Mario is savvy enough to say that things can get worse. Then Luigi asks how...
  • "Escape from Koopatraz":
    • Mario, Luigi, Toad and Gramps attempt to dig their way out of Koopatraz... only to wind up directly underneath Warden Koopa's bed.
    • The ridiculously biased Kangaroo Court Koopa presides over in the first scene is pretty funny.
      Toad: Why do I get the feeling he's gonna throw the book at us?
      (Koopa LITERALLY throws a law book at them)
  • Koopa praising himself in "Brooklyn Bound":
    Koopa: The courage beyond compare, the bravery beyond description, I praise this great hero, the superior fiend... me, Koopa Khan the magnificent! If I didn't deserve this, I wouldn't give it to me.
    • Believing that the Mario Bros. have gone back to Brooklyn, Koopa Khan captures Princess Toadstool, who tells him that he won't get away with it.
      Koopa: Who's gonna stop me? Your plumber pals have run out on you.
      Mario: (hiding above) WRONG AGAIN, LIZARD BREATH!
      Luigi: I thought we were gonna sneak up on 'em.
      Mario: ...I forgot!
  • From "Pirates of Koopa" when the Mario Bros. are at the bar:
    Mario: Remember, Luigi. You gotta act tough!
    Luigi: (slams hand on counter) I'll take a milk!
    Mario: ...I-In a dirty glass!
  • Koopa threatening to repossess his Koopa Troopas' shells in "The Pied Koopa".
  • In "Hooded Robin and his Mario Men", the team (plus the eponymous Robin Hood expy) need to lure Fryguy away from the door to Koopa's treasure vault. Princess Toadstool's plan: create a "Frygal" to distract him. Hooded Robin's ability to mimic voices makes him the perfect candidate to provide the voice – and body – for Frygal. The entire exchange is hilarious.
    • In the same episode, Koopa saying one of Toad's lines in his voice.
  • "The Adventures of Sherlock Mario":
    • King Koopa/Professor Kooparity is left speechless when the captive Herlock Solmes effortlessly deduces his plan. Koopa then angrily demands to know which one of his mooks spilled the beans.
      Tryclyde: Heh heh! I bet it was Mouser!
      Mouser: Koopa Troopa's the one who did it!
      Koopa Troopa: You're the one, you great, stinking liar!
    • After pulling off his plan, Kooparity asks Solmes what he thinks of him now.
      Herlock Solmes: Elementary, my dear Kooparity. You're a cross between a lizard and an inferior species of toad. Your brain is smaller than a peanut. You got the lowest grades in your school and hold the world record for flunking kindergarten the most times. When you were little, the other Koopas nicknamed you "Lizard Lips" and never let you play with them. You were a naughty lily-livered bully boy and wet the bed until you were twelve.
  • After Mario and Luigi are kicked out of plumbers' school.
    Mario: I can't give up now, Luigi. I was born with a plunger in my hand!
    Luigi: I know. Boy, it was awfully hard on mom.
  • The Koopa Troopa that was dressed as a woman in "The Ten Koopmandments", fake boobs and all.
  • In "Jungle Fever", Mario attempts (and fails spectacularly) at trying to find a loophole.
    Mario: Let's take a lunch break!
    Princess Toadstool: Oh, Mario! You just ate three guavas, four breadfruit, and two bananas!
    Mario: (scowls) If food isn't pasta, it doesn't count.
  • In "Quest for Pizza", Mugga being revolted at the sight of a comatose Mario.
    Toad: Yeah, I know. He's hard to look at, but can ya' help him anyway?
  • "Toad Warrior":
    • Toad attempting to provoke a Bob-Omb into lighting itself.
      Toad: (with a smug grin) Hey, Bob-Omb! Did anyone ever tell you you were uuuuuuuuuu-gly?! I've seen better-looking bombs after they exploded! I bet you have to sneak up on a match just to get a light!
    • When Toad urges Mario to check his rear-view mirror...
  • Mario calling Luigi out on his cowardice in "Mario of the Deep".
    Mario: Luigi! Are you a man, or a Mouser?!
    Luigi: Neither. ...I'm a chicken of the sea!

    Live-Action Segments 
  • In the live-action episode "Magic's Magic", Luigi gets stuck inside a magic hat and guest star Magic Johnson tries to get him out, but he can't remember the right magic word. "Abracadabra" doesn't work, "Open Sesame" doesn't work, but Mario gets Luigi out by shouting the only magic word he can think of: "PLEASE!"
  • In the live-action episode "Baby Mario Love", when an expy of Diana Ross of the Supremes asks Mario and Luigi to fill in as her backup singers, which means having them dress in drag and sing the Supremes' "Baby Love." At one point, they begin shoving each other before the Diana Ross expy breaks it up.
  • The live-action skit "Will the Real Elvis Please Shut Up!". All of it.
  • Sgt. Slaughter calling the Mario Bros. to inform them he's on his way to pick up his steam cabinet.
    Sgt. Slaughter: You better have my steam cabinet fixed. I'm stuck in traffic on the Brooklyn Bridge. But I'm takin' a shortcut. I'll be right over.
    Mario: How do you take a shortcut off the Brooklyn Bridge?
    Sgt. Slaughter: It helps if you have a Sherman tank, Bozo!
  • Dr. Frank N. Stein from Pennsylvania.
    Luigi: (beat) You meatball. It's not Pennsylvania; that's Transylvania!
  • The live-action skit "Bonkers from Yonkers". Mario gets hit in the head by a pipe, and now thinks he's a chicken. Luigi calls Dr. Sigmund Fruitcake to get Mario back to normal, but now Mario thinks he's a monkey instead. When Luigi calls Dr. Fruitcake, he asks him to turn Mario back into a chicken. The reason?
    Luigi: Well, to be honest with you, we could use the eggs.
  • In "Fortune Teller", Mario, who's full of energy, reads to Luigi, who's half-asleep with a cup of coffee, the prizes of the Big-Time Celebrity Prize Sweepstake Giveaway.
    Mario: The third prize is an all-expense paid trip to Italy... with Sophia Loren.
    Luigi: Sophia Loren...
    Mario: Now, second prize is a tour of Hollywood, California... with Annette Funiciello!
    Luigi: Annette Funicello?
    Mario: (shrugs) Funicello; that's the same one. The first prize, an evening on the town... with Madonna!
    Luigi: Madonna?!
    Mario: Now, the grand prize... (gestures wildly) is milk and cookies, with...
    Both: (double take) ...Scott Baio?

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