Amazon Chaser: King Shark really likes Killer Frost's feisty nature, especially when she kicks his ass.
Anyone Can Die: It isn't called a suicide mission for nothing. KGBeast, King Shark, and Black Spider don't make it out alive. Frost may also have died as a result of being in a car thrown by Bane. And, while it's likely she will survive, Waller is about to be killed by Deadshot at the end of the movie.
Ascended Extra: Deadshot's prior appearances in the games had him as just one of the many Rogues Batman had to apprehend. In City and Origins encountering him was even delegated to Side-Quests. Here, he's a primary character and is additionally exceptionallymore competent than he was in the games as well.
Authority Equals Asskicking: The leader of the squad sent to kill Riddler in the opening holds his own surprisingly well against Batman.
Axe Crazy: Everyone, but Harley and Joker in particular.
Badass Normal: Quite a few of these running around. Batman, Harley Quinn, Deadshot, Captain Boomerang, Black Spider, the one member of the hit squad that lasted a few minutes in a fight with Batman (Waller got her money's worth from that guy), and Joker all count.
Batman Gambit: Not from the Trope Namer himself, though posing as Black Spider might count, but from Amanda Waller. Batman even calls her on it at the end of the film. Waller knew the convicts would go rogue once in Arkham, and was counting on that as a diversion for a lone assassin to go after The Riddler. She knew if she sent the whole team with the objective of killing The Riddler, it would arouse their suspicions as to why. However, despite "breaking a lot of eggs to make an omlette", Batman points out the flaw in her plans by calling her "a messy cook", as Riddler got away.
Bloodless Carnage: Despite all the very bloody tropes above, the signature Suicide Squad detonating heads are rather clean. If blood is shown at all, it only flies through the air and doesn't actually splatter onto the surroundings.
Butt Monkey: Captain Boomerang gets no respect, a lot of Amusing Injuries, and is basically the go-to guy when someone needs to be made a fool of.
The Brute: King Shark is a classic example. KGBeast would've been, too, if he'd not assumed Waller was bluffing about the explosive implants.
In one scene, the Squad wakes up restrained hand and ankle to a series of chairs. Everyone, that is, except Black Spider, who promptly appears with a knife to Captain Boomerang when he begins shooting his mouth off.
Ironically, Arkham itself is, for once, not one, and has a positive reputation where security is concerned, judging by the Squad's reaction to finding out their mission. The asylum is depicted with fairly reasonable security measures—Black Spider, Killer Frost, and Captain Boomerang are nearly caught during their disguised entrances to the facility, for example.
Casual Danger Dialogue: While The Joker and Deadshot are fighting in the back of a helicopter, while Harley (who can't fly) takes the stick:
Joker: "You know she's going to kill us, right?"
Catch Phrase: Harley Quinn has one: "Yahtzee". Turns out she got it from the Joker.
Harley gets caught by Batman while looking for her huge mallet. She later finds it in the evidence locker. And the Joker was hiding his dirty bomb inside it.
When Harley flips out and unloads a pistol at the Joker, she aims through one of the air holes and fires several times, creating a slight hole in the wall. Joker later discovers that this spot is exactly where the door wiring runs.
Close-Call Haircut: In her fight with Batman at the toy store, Harley dodges a couple of batarangs. Though they miss, one clips the left tail of her jester hat, slicing off the ball at the end.
Conservation of Ninjutsu: Batman vs. half a dozen mooks with automatic weapons; no problem. One mook with a knife; problem.
Several of the rooms seen in the game are identical to how they appear in Arkham Asylum (Without the obvious damage the game's event causes on them). In particular, the room containing the Joker's cell (Circular room lined with high security cell and a tower in the middle - in the game it is where Batman meets Clayface), and the underground ruin room where the team re-joins Killer Frost and the Riddler.
Some of the things Batman does refer to the Arkham games, in particular using his cloak to strike an opponent when facing a knife wielding mook, which is how the player deals with blade-enemies in the game.
He also uses explosive gel, an Arkham mainstay, by shooting some in Bane's face and detonating it.
The entire fight against Waller's team in the opening is very similar to the games' Freeflow combat system in its execution to the point where as mentioned above, some of the moves are lifted straight up from the games. Batman moving from foe to foe, vaulting over some, attacking them all - with the occasional gadget, till they are all down is very familiar to anyone who has played the games.
Batman uses electric knuckle dusters, similar to the gauntlets he got off Electrocutioner's body in Arkham Origins.
Batman rides the back of Bane for a moment, as he stumbles and strikes things and inmates blindly. Batman pulls this move against most large enemies in the games, including yes, at one point, Bane himself.
He briefly uses a version of Detective Vision, which lets him see who is riding away from Arkham in the Helicopter. Unlike the games' it doesn't give him the outline of their skeletons, but does show their pulse and makes his eyes glow when he uses it like in the game.
And of course, Batman uses his grapple gun to get to the Batwing, which is how he gets in it every time in Origins when it is not landed.
Continuity Snarl: Bane is portrayed as an inmate of Arkham Asylum. This is in contrast to the Batman: Arkham Asylum video game, in which Bane is an inmate of Blackgate who is brought to Arkham shortly before the events of the game for research on the TITAN formula.
The Evil Genius: Harley Quinn, who knows the layout of Arkham, knows the passwords, and knows how to rig the security cameras to loop previous footage.
Evil Versus Evil: The Squad are all murderous criminals who are forcibly "recruited" to deal with an issue caused by the Riddler. And after the Joker takes over as the Big Bad, the big climax is a fight between him and Deadshot.
Exactly What I Aimed At: Batman aims a grapple at Zsasz while the latter is holding a woman hostage, hitting the wall being him. Zsasz silently gloats, but then Batman gives it a good tug and whacks Zsasz with a chunk of wall.
Explosive Leash: Powerful nanotech explosives are injected into the necks of the Squad, allowing Waller to both track them and kill them if need be.
I Am A Humanitarian: King Shark is found at the beginning of the movie in a house. He has several bodies stripped and hung from the ceiling like slabs of meat at a butcher shop, plus a bathtub full of blood he was bathing in, heavily implying he was feeding on them. He also attempts to eat Killer Frost upon first meeting her.
Imperial Stormtrooper Marksmanship Academy: At one point, a group of Arkham security guards armed with submachine guns have King Shark charging at them...and not one of them opens fire before he's slamming through their ranks.
Improbable Aiming Skills: Deadshot and Boomerang's dart game. Boomerang also has the distinction of somehow managing to boomerang a lunch tray. Joker proves to be a crack shot with a pistol, too.
It Works Better with Bullets: When he's held at gunpoint by the Joker, Deadshot confidently states that Joker has emptied the clip and, once he fires and nothing happens, will get his face beaten in. Joker takes the time to check the barrel (by pointing it at his face), then fires it into the ceiling, giving Deadshot enough time to sucker punch him and steal back his Arm Cannon which Joker had managed to swipe during the electroshock confusion.
Joker Immunity: The Joker, of course, and Two-Face walks away from Killer Frost freezing his head, even though she took out a coroner with the same move.
Kick the Dog: During the opening credits, a blink-and-you'll-miss-it shot shows that Killer Frost took this trope almost literally by freezing a bystander and her dog.
Kill and Replace: A non-lethal example, in that Batman knocks out Black Spider and takes his costume. However, it still turns out lethal for Spider when Waller detonates the explosive collar on his neck.
The scene when Harley bursts in a toy shop in search of a Chatty Boo Boo doll bears a resemblance when Catwoman bursts in and vandalizes Shreck's Department Store in Batman Returns.
Cobblepot eating raw fish is another reference to the same movie.
Also from Batman Returns, Batman saves a female hostage from Zsasz by shooting his grapple-gun's hook into the brick wall behind them, then quickly retracts it so a chunk of the wall whacks the psycho in the back of the head.
The sex scene between Harley and Deadshot and their relationship in this movie is a reference to a similar sex scene between them and their relationship in the Suicide Squad title in the New 52. The rivalry between Deadshot and Captain Boomerang comes from the same title, as well.
When Harley's rooting through one of Joker's crates in the evidence locker, she tosses out some masks based on the ones that Joker's bank robbers wore in the opening of The Dark Knight.
She also tosses out a Laughing Fish.
When Waller is taunting Batman at the end, she declares, "Checkmate," which is also a subdivision of Task Force X.
Never Trust a Trailer: The trailers reveal that the Joker gets his hands on a dirty bomb and makes it seem like that's the reason the Squad's are going to Arkham. In fact, the squad is there to (supposedly) steal a flash drive containing the names of every potential member of the Suicide Squad, while Batman is the one trying to find the bomb.
Noodle Incident: Apparently Joker used a Batman costume for a train heist once.
Obfuscating Stupidity: Harley Quinn plays at being the ditz, but she's the one who knows the passwords and layouts.
Oh Crap: The expression on all the Squad members' faces when they see Batman, realize Batman is nearby, or pretty much at any reference to Batman whatsoever.
The Password Is Always Swordfish: The warden uses his birthday (backwards) for most of the door codes, which makes it easy for Harley to break into the personal storage areas.
Pass the Popcorn: When Deadshot and Captain Boomerang get into a fight that could potentially expose them, King Shark's chief concern is seeing which of them will win.
King Shark: (Pumping his fists in the air) U.S.A.! U.S.A.!
Put on a Bus: Despite the presence of the Squad, Deathstroke, Rick Flag, and Bronze Tiger are nowhere to be seen with the only people from the subplot in The Stingers of Origins and Blackgate being Waller herself and Deadshot.
Race Against the Clock: Batman is racing to disarm Joker's bomb before it kills an untold amount of people in Gotham. The Suicide Squad, meanwhile, are attempting to complete their mission and get the Riddler to disarm their own bombs so they can get out before the Joker's bomb detonates.
Race Lift: In the comics, King Shark is a real ocean-dwelling humanoid shark that came into existence through magical means. In the film, he appears far more like a reskinned Killer Croc with a cybernetic jaw.
Sacrificial Lamb: KGBeast is recruited by Waller like the others, but when he tries to walk out on the job the bomb in his neck goes off. This shows that Waller is the one who holds the power over the Squad.
Ship Tease: Killer Frost has some surprisingly nice moments with King Shark.
Show Some Leg: Killer Frost removes her top when being snuck into Arkham as a corpse, distracting the coroner long enough to give him a Kiss of Death. Later on, Harley does the same to distract a security guard while the rest of the crew pops open a grate to allow King Shark in. Though the guard realizes it's Harley, he's too late to avoid becoming lunch.
Spotting the Thread: Batman merely needs five seconds to realize he's watching the security footage from the previous day.
Take That: When the Suicide Squad find out what the Joker is planning.
Killer Frost: That gives me the chills. Captain Boomerang:Ice Puns? Really?
Token Good Teammate: Black Spider, the vigilante. Becomes doubly true once Batman replaces Black Spider.
Too Dumb to Live: When Amanda Waller informs the Suicide Squad they are wearing Explosive Leash, KGBeast accuses her of bluffing, reasoning they wouldn't go through so much trouble just to kill them. Waller dares him to test her warning. He does. Predictably, this costs him his life.
Too Kinky to Torture: When Riddler straps the Suicide Squad into chairs for some impromptu electroshock therapy in order to disable the explosives in their necks, most of them are writhing in pain. Except for Harley Quinn, who's laughing all the while.
Villain Ball: The villains had no reason to fight each other over the escape helicopter - it was large enough for all of them and together they had better chances of reaching it. But, of course, there's No Honor Among Thieves. Joker is even worse for trying to kill the pilot, when he is inside the helicopter himself.
Villainous Friendship: Shark and Frost actually seem to get along rather well after meeting each other. Although brief, she is actually quite upset when Shark's head is blown up in the attempt to deactivate the explosive collars.
What Happened to the Mouse?: The last we see of Killer Frost, she's inside a car that's flung into the side of the Asylum and exploded. Did she die or did she make it out in time? To a lesser extent, Captain Boomerang is left stranded in Arkham after Deadshot escapes in the helicopter and is never seen again.
Why Did It Have to Be Snakes?: King Shark isn't good with heights. It starts out as a one-scene gag, but pops up later when the Suicide Squad has to cross a bridge over a long drop.
Wire Dilemma: When Batman finally has time to disarm the bomb, the case has a helpful label indicating that he should cut the red wire. Naturally, all of them are red.
Your Head A Splode: What will happen to any member of the Squad should Waller detonate their Explosive Leash, as demonstrated by KGBeast when he tries to leave. Black Spider and King Shark later fall victim to them.