Referenced By: Super Mario Bros.
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- In one episode of Calvin and Hobbes: The Series, Andy is shown playing a video game with a plumber for a protagonist.
- Turnabout Substitution references the series.
- My Little Castlevania mentions Super Mare Sisters when referring to something that's hard. (No, not that.)
- My Stupid Reality also references the series.
- In Past Sins, Nyx/Nightmare Moon deals with a Scorpio by swinging it several times, then launching it into the Everfree Forest.
- In Yu-Gi-Oh! GSTART, Kohaku Yudin's Pixel HERO Deck includes multiple Monster Cards that reference this series (among others), including Red Hammer (Mario), Green Jumper (Luigi), Crown Parasol (Peach), Muscle Kong (Donkey Kong), Red Blazer (Fire Mario), and Red Freezer (Ice Mario). According to the author, Kohaku's design also includes a few cues from Mario, such as his red shirt, white gloves (shared reference to Sonic), and red M-shaped fringe in his hair.
Film - Animated
- Wreck-It Ralph has a few references to the series, despite not having the brothers themselves.
- Ore no Imōto ga Konna ni Kawaii Wake ga Nai has a game labeled "Super Mario Sisters".
- Dino Attack RPG: Doctor Marco Martinet started a reference to the Dr. Mario games before expanding to being inspired by the Super Mario Bros. series as a whole. His brother, Louie Martinet, is based upon Luigi, his cousins Warner and Wallace are inspired by Wario and Waluigi, and his former love interests Pearl and Primrose are inspired by Princess Peach and Pauline, respectively. Additionally, the sports Martinet participated in his youth (including going to the Olympics) are references to various Mario spin-off games.
- Dr. Alvin Gadd, a XERRD scientist, is named after Professor Elvin Gadd from Luigi's Mansion. One of Alvin Gadd's inventions, the Flight and Laser Ultra Destruction Device, is based upon one of Elvin Gadd's inventions, the Flash Liquidizer Ultra Dousing Device.
- Rosalie Mercedes is based upon Princess Rosalina from Super Mario Galaxy. Her first appearance flying into the final battle with a fleet of T-1 Typhoons following her and destroying Fright Knight windships was inspired by the cutscene in Super Mario Galaxy'' featuring Rosalina flying into Bowser's Galaxy Reactor and destroying the airships that guarded it.
- One comment on the comm chatter showed someone finding a technicolor butterfly and declaring it "hi-technical."
- One comment thought the Maelstrom had been created by a chaos heart and needed pure hearts to defeat it, referencing the Void created by Count Bleck in Super Paper Mario.
- Astérix and Obelix XXL2 have several references to the series:
- Even before entering Las Vegum proper, you'll face the first special Roman soldier. He has a big moustache, a helmet with a red Roman Eagle shaped like a M, and turtle shells as shoulder pads. He's equipped with a FLUDD device (Super Mario Sunshine was the most recent entry of the main series at the time of XXL 2's development). When defeated, these Mario Soldiers emit a muffled "Mamma mia!" They get some statues on the Arc de Triomphe, and small flags near the Eiffel Tower.
- In one of the employee back rooms, a menhir colored like a Yoshi egg◊ is clearly visible.
- A giant Super Mario World-like wall is visible from some spots in Lutetia. If you look around from the top of the Eiffel Tower, you'll also notice a few of the characteristic striped hills.
- There are several small portraits of Toad◊ on the walls in Venetia.
- Also in Venetia, you can see rows of small flags with red Yoshi eggs and mushrooms.
- A transition room in Venetia has the faces of Mario and Luigi◊ near the two big buttons. The buttons themselves are of the same red and green as the Mario brothers.
- Later in Venetia, in the square inside the giant dark hall, you can see several statues, among them Princess Peach and a Shine Sprite (you can find two more a little later).
- In the large courtyard at the beginning of WCW, there's a big green pipe◊. You can enter it and find an entire Mario-themed area. There are floor mosaics of carnivorous plants, tiles of mushrooms, flowers, banana peels and Shyguys, unstable platforms shaped like Shine Sprites and POW blocks, and Thwomp-like devices◊ (although they are designed to make you fall rather than crush you).
- Also in the exterior areas of WCW, there are two◊ small◊ Mario-esque artificial hills that stand out evidently.
- At the end of WCW, before entering Pirate Island, you can notice wall tiles reproducing Super Mario blocks.
- On the beach of Pirate Island, you can find towels with the crab enemy from the original Mario Bros.. A few Starmen can also be seen on the sand (although they may be just cartoony starfishes).
- Near the end, after the section with the Zelda and Link statues, you enter a waterway whose walls are decorated with Bloopers and Cheep Cheeps◊.
- The description for the Spiny Shell item in Disgaea 2: Cursed Memories: "Jumping on this one is a bad idea."
- Duke Nukem 3D has Mario playing Mario Kart.
- An area in Kingdom of Loathing has you fighting Goombas, Koopas, Buzzy Beetles, Bullet Bills, and Bloopers. The Buzzy Beetles only appear after you ascend once, a reference to their appearances in the New Game+ of Super Mario Bros. 1.
- Also, one part of the fight against a Goomba has this message:
- Also, the fumble message for one monster has it eat several mushrooms, only for it to grow and hit its head on the ceiling.
- Another adventure quotes the "Your Princess Is in Another Castle" spiel.
- The description for the penguin shorts state that it allows you to slide around on your ass, which is pretty much what you must do to beat the penguin in a race in Super Mario 64.
- The cyborg stompin' boot has a couple qualities that imply it's a homage to the Kuribo's Shoe.
- Rescuing the King leads him to say "I'm sorry, adventurer, but the king is in another castle."
- An Idle Animation in Epic Battle Fantasy 3 has Natz playing Mario on a Nintendo DS.
- Enough Plumbers is very inspired by the series.
- As was Super Giana Sisters, which declared "The brothers are history!" Nintendo responded with a lawsuit.
- Forum Fantasy has Bowser as a boss.
- I Wanna Be the Guy has several references to the series, least among them being a recreation of the Final Boss of Super Mario World... with Wart and Dr. Wily.
- In Lollipop Chainsaw, Juliet's super-mode has her change a variety of different colors, and a special music plays ala Mario once he gets a star.
Nick: Alright, this is all too surreal. If a little dude in a red cap and blue overalls pops out at me I am seriously gonna vomit all over myself.
- During the Fulci Fun Center stage:
- The emblem reward for getting ten kills in a row while having no killstreaks equipped in Modern Warfare (hence worth trying only for this) is an 8-bit image of Capt. Price that looks exactly like Mario's Super Mario Bros.. 1 sprite.
- Neptunia has several expies from the series.
- Osu! Tatakae! Ouendan: The plumbers in "Bambina" are obvious expies of Mario and Luigi.
- Punch-Out!!: Mario makes an appearance as the referee.
- Vector Thrust: Several of the campaigns are set against the backdrop of the Mushroom Kingdom. In a rather dark twist the "Mushroom" in the name stems from the massive nuclear war that took place there.
- Everybody Edits has a few.
- There is a pipe package that reads, "Factory outlets or italian [sic] plumbings?" as a reference to that Mario is an Italian plumber.
- An official Christmas level shows off music blocks containing the first few notes of the Super Mario Bros. theme, along with text that says "It's-a-me, Mario!"
- The coin sound is taken directly from Super Mario World.
- Square Root of Minus Garfield:
- Rice has the titular rice being sucked through a Warp Pipe.
- The Bonus Chance from Super Mario Bros. 2 is among a long list of things that sound like "minus" in They Just Won't Stop!
- Mariofield edits a Christmas-themed strip. In the original, Garfield gives Jon the "Box O' Mystery", which turns out to be empty. In this version, the box is a Question Box and it contains a Poison Mushroom.