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Some form of Applied Phlebotinum results in one or more main characters regressing to an earlier age. It may be the result of a freak accident, a plot on the part of the bad guys to incapacitate our heroes, or done on purpose so the characters can relive their childhood (at least temporarily).

For some reason this plot is often used in Fan Fic, where it's called a De-Aging story.

When someone dies in his rejuvenated state, expect him to turn back old immediately.

When a Fountain Of Youth effect doesn't get reversed and the younger actor replaces the older one in the cast for good, it's also an example of The Nth Doctor.

Contrast with Thirteen Going On Thirty and Plot Relevant Age Up. Compare Really Seven Hundred Years Old.

Examples:

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Dawson BabiesYoungstersFirst Snow
Fix FicFanfic TropesFreaky Friday Flip
Mandy's Law Of Anime Gender BendingShape ShiftingThirteen Going On Thirty
Animation Anatomy AgingAging TropesGlam Of Shazam