Voiced by: Bruce Greenwood
- The Fettered: Unlike Red Hood. He states he's been tempted to kill the Joker, many, many times, but he's afraid that if he starts killing he will take a step too far and never stop.
- Heartbroken Badass: A non-romantic version. Jason's death and subsequent downfall into villainy left him broken and angry at himself for not being able to save Jason and leaving him with the wish that he could have done more for him if he had the chance.
- My Greatest Failure: Jason's death and his downfall into becoming Red Hood.
- Save the Villain: Saves Red Hood more than once throughout the film. His moral code is so strong that even saved the Joker.
Voiced by: Neil Patrick Harris
- Butt-Monkey: Batman keeps leaving him behind.
- Deadpan Snarker: "I'm chatty. It's part of my charm."
- Game-Breaking Injury: Injuring his leg while he and Batman chase after Red Hood both writes him out of the story and references him wearing a brace on his leg in the original comic.
Robin II/Jason Todd
Voiced by: Vincent Martella
- Anti-Hero: Flashbacks show him more willing to injure people than Batman would like.
- Dead Sidekick: He's killed by the Joker in the opening.
- Leotard of Power: In his early Robin days.
- Posthumous Character: The story opens with his murder by the Joker.
- Time-Shifted Actor: Young Jason is voiced by Alexander Martella. And then there's Jason as Red Hood.
Voiced by: Jim Piddock
- Not So Stoic: Alfred is in top form, unflappable in the face of everything Batman-related. But when the computer confirms that the Red Hood is Jason, he drops the tray he was carrying, and can only stare at the screen in numb recognition.
Red Hood/Jason Todd
Voiced by: Jensen Ackles
- A Million Is a Statistic: The crux of Jason's grievance with Batman. Despite the "entire graveyards" worth of people that the Joker has killed, Jason was hoping he'd be special, that he'd be the one Batman actually avenged.
- Anti-Villain: His first act upon assuming control of the various gangs is to make children untouchable to the drug trade, and he only ever targets criminals in the course of his various activities. As he tells Batman during their first real chat, he's actively attempting to control the criminal element of Gotham, but we don't find out why until the climax.
- Asskicking Equals Authority: He sets himself up as the drug kingpin in Gotham and rival to Black Mask by showing up at a meeting of the "heads" of the organizations under Mask's thumb and giving them a bag full of the heads of their lieutenants.Hood: I did that in two hours. Wanna see what I can do with a whole evening?
- Back from the Dead: Courtesy of Ra's al Ghul and the Lazarus Pit.
- Big Bad Ensemble: With the Joker.
- Came Back Wrong: According to Ra's al Ghul. The man himself throws it back in Batman's face, suggesting his current activity is somehow easier to digest if the Lazarus Pit "turned [him] rabid".
- The Chessmaster: The movie's entire events were all a plan to get the Joker out of Arkham and put him in a situation where Batman would have no choice but to kill him.
- Cold Sniper: He kills three people from a significant distance without touching Batman or Nightwing standing to either side of them.
- Combat Pragmatist: Though he has plenty of gadgets up his sleeves, he's not afraid to use anything in the environment to his advantage in a fight, and, as noted under The Unfettered, is willing to use any methods necessary to gain an advantage.
- Complexity Addiction: His ultimate plan to get Batman to realize that sometimes Murder Is the Best Solution, specifically regarding irredeemable people like The Joker. Instead of just finishing The Joker off himself and using that as the ultimate symbol of his new pragmatic approach being more effective than Batman's idealistic approach, he goes to extra trouble to get Batman to abandon his Thou Shalt Not Kill rule and finish The Joker off.
- Cool Helmet: It explodes too.
- Crazy-Prepared: To be expected, as a member of the Bat Family.
- Faux Affably Evil: He has a rather snarky, personable, and somewhat laid back personality, is cool under fire and logical with always a few tricks up his sleeves and sharp tongue to match. However, this belies his true nature of an angry, violent, young man with serious emotional and (possible) mental issues.
- Guns Akimbo: Likes to to use a pair of handguns.
- The Heavy: The main antagonist and the one who drives the plot along for the majority of the film.
- He Who Fights Monsters: He's vigilante with no hesitation against killing, and his entire plan consisted of forcing himself into the criminal underworld to keep Gotham's criminals in line.
- Irony: There's the fact that his actions are based around heroism, but are so brutal that he is a firm villain. But there is also a bit of symbolic irony in a bat family member becoming a new Red Hood, with the original being the original villain alias of who else but The Joker.
- Knife Nut: After the guns, before the fists, he has a knife that can cut Batman's cables.
- Knight Templar: Believes that everything he has done, every life he has taken, has been in the name of bringing order and stability to Gotham.[To Batman] "Yes. You can't stop crime. That's what you never understood. I'm controlling it. You wanna rule them by fear. But what do you do with the ones who aren't afraid? I'm doing what you won't, I'm taking them out!"
- Not So Different:
- To Batman; A masked vigilante with a penchant for the Stealth Hi/Bye, who preys on criminals and develops a reputation of dread, a small arsenal of various weapons and explosives on his person at all times, listening devices installed in Black Mask's office, an expert marksman with a penchant for firearms instead of thrown weapons, even a grappling line that sees use in their final battle; Really, the only thing that differentiates the two is Hood's willingness to kill. This is the first big clue as to his identity.
- To the Joker; Aside from assuming his old alter-ego, he captures the Joker, beats him with a crowbar, and then tries to finish him off in a building rigged to explode.
- The Reveal: Blood analysis shows that he's Jason Todd.
- The Unfettered: Will use whatever methods necessary, no matter how brutal or violent, to achieve his end goal of "cleaning up Gotham." Despite that, he still won't tolerate drug dealing to children, even if it would increase his cut of the profits.
- Woobie, Destroyer of Worlds:He was once the partner of Batman and a former juvenile delinquent whose hard background, brutal death at the hands of the Joker, and subsequent resurrection drove him over the edge to become the murderous Knight Templar Red Hood.
- Would Not Hurt A Child: Threaten to kill any of the drug lords if they sold drugs to children.
Voiced by: John DiMaggio
- Adaptational Badass: In most adaptations, Joker is more of a schemer than a fighter, or at best a desperate scrapper when cornered. Here, he quickly and effortlessly murders a room full of armed mob bodyguards while handcuffed. He's got a sturdier build than he is usually portrayed with, and seems more capable of getting his own hands dirty.
- Ax-Crazy: As usual. The movie opens with him beating Jason Todd with a crowbar. Almost to death. Then the bombs finish it.
- Big Bad Ensemble: With Red Hood
- Darker and Edgier: Kind of; this version of The Joker is more thuggish, but does the Tommy Udo version of the Giggling Villain.
- Deadpan Snarker: His humor and delivery are more sarcastic and measured than his usual bonkers antics. He still giggles like a deranged schoolgirl, but less frequently than many adaptations.
- Evil Is Hammy: This aspect of him hasn't changed.
- Evil Is Not a Toy: Black Mask in a moment of desperation, breaks Joker out of Arkham in exchange for him killing the Red Hood. Naturally the Joker betrays him almost immediately.
- Evil Sounds Deep: Highly unusual for the Joker, but this plays into his Darker and Edgier characterization.
- Hate Sink: The Joker from Batman: The Animated Series was a monster to be sure, but his despicableness was counterbalanced by a bombastic sense of showmanship and Comic Relief. Conversely, this Joker is less cartoonish and much more thuggish, despicable and realistically monstrous than usual.
- The Heavy: In the greater scope; The events of this movie are entirely his fault, and he doesn't feel a drop of remorse for it.
- Karma Houdini: At the end of the day he suffered no comeuppance for his villainy, and he had an utter blast watching Batman's struggle with Red Hood.
- Monster Clown: Moreso than other incarnations.
- Pretender Diss: Says his time as the Red Hood was "Classy, more flashy maître d' than motorcycle fetish" when initially questioned by Batman and Nightwing, and later to Red Hood himself.
- Sadist: He takes immense joy out of the suffering he inflicts on others.
- The Sociopath: He has absolutely no regard for human life, including his own.
- Would Hurt a Child: His first scene shows the Joker beating a teenage boy nearly to death, and then finishing the job with a bomb."Now, that was rude. The first boy blunder had some manners. I suppose I'm going to have to teach you a lesson so you can better follow in his footsteps. (beat) Nah, I'm just gonna keep beating you with this crowbar."
Voiced by: Wade Williams
- Bad Boss: When ranting about Red Hood, he's willing to randomly punch his subordinates, notably the new guy who isn't used to his appearance.Black Mask: *suddenly calm* You. New guy. Don't be nervous kid, but if you keep staring at me like that, I'm gonna cut your eyes out.*Black Mask lays him out*
- Big Bad Wannabe: Prior to its theft by Red Hood, AMAZO was to be Mask's ticket into international arms deals, a step-up from his current "bottom-feeding garbage" of small scale drug and weapons trafficking. Later, after a direct attack from Red Hood, Mask decides to get out of the "cross-fire" by breaking the Joker out of Arkham, which goes about as well as could be expected.
- Captain Obvious: During a rant about taking down the Red Hood.Mask: Maybe my last request was too mired in subtlety. I want this man dead. When I say "dead", I mean "seriously dead". Beaten. Broken! HIS-HEAD-MOUNTED-ON-MY-WALL KINDA DEAD!Ms. Li: Understood. We'll be taking further precautions at every transaction.Mask: Screw that! Time he learned that this is a contact sport. We're going on offense. Rough up his business; something big! Something loud! When he shows up to shut us down, have a party waiting for him! And when I say "party" I actually mean a 'whole lot of people who are gonna KILL HIM!Li: I figured.Mask: Just being clear.
- Even Evil Has Standards: He's clearly uncomfortable with hiring the Joker. He has good reason to feel that way.
- Genre Blind: He trusted the Joker. It doesn't get much worse than that.
- On the other hand, Black Mask didn't ''want'' to hire the Joker, and only did so out of desperation.
- Hair-Trigger Temper: Prone to beating up his subordinates for little to no reason. Though, to his credit, he is less homicidal than the comic version.
- Large Ham: His mannerisms are based on Al Pacino in full overblown gangster mode.
- Unwitting Pawn: Of the Red Hood; Black Mask is the only one with the connections to spring Joker from Arkham, which was Red Hood's real goal all along.
Voiced by: Kelly Hu
- Deadpan Snarker: Emphasis on deadpan.
- The Dragon: To Black Mask. She doesn't do a lot onscreen, but she's apparently the one who organizes Black Mask's heists and plans out said heist's finer details.
- Gender Flip: Mr. Li in the comic.
- Not So Stoic: She loses her composure while the Joker is holding her, Black Mask, and several drug dealers hostage, and is preparing to set them on fire.
- Number Two: To Black Mask.
- Only Sane Man: Black Mask is too enraged to think straight and everyone else is too scared of their enraged boss to be productive.
- Spared by the Adaptation: Mr. Li is killed by Jason in the original story, whereas Ms. Li is fine by the end of the movie.
- The Stoic: Takes Black Mask's tantrums without so much of a change on her face.
Ra's al Ghul
Voiced by: Jason Isaacs
- Affably Evil: He states that he never resorts to violence or killing unless it's for a "greater cause" and is deeply remorseful that his hiring of the Joker was ultimately what lead to Jason's murder; his attempt to fix things via Lazarus Pit resurrection is what sets off the main plot.
- The Atoner: After Jason's death, Ra's chooses not to oppose Batman again, and tries to resurrect Jason to try and repair the damage he inadvertantly caused.
- Even Evil Has Standards: He is greatly upset by the death of Jason, calling it both unnecessary and brutal. Batman points out he never had a problem killing before. Ra's counters by pointing out he has killed for a greater good, never for laughs as Joker did.
- My God, What Have I Done?: After his actions cause Jason's death, and again, after hearing of Jason's actions as the Red Hood, following Ra's raising him from the dead.
- Unwitting Instigator of Doom: Twice. First, when his hiring of the Joker was ultimately what lead to Jason's murder. Second, when he did try to resurrect Jason to try and repair the damage he inadvertantly caused, but his actions only made things worse.