Batman/Bruce Wayne (Bruce Greenwood)
- The Atoner: Following Jason's death.
- Badass Baritone: Courtesy of Bruce Greenwood.
- Berserk Button: Don't bring up Jason Todd, especially if you happen to be the Joker.
- Don't force him to kill anyone. Even if that person deserves it.
- The Fettered: Unlike Red Hood.
- Genius Bruiser
- Heartbroken Badass: A non-romantic version. Jason's death and subsequent downfall into villainy left him broken and angry at himself for not being able to save Jason and leaving him with the wish that he could have done more for him if he had the chance.
- My Greatest Failure: Jason's death and his downfall into becoming Red Hood.
Nightwing/Dick Grayson (Neil Patrick Harris)
- Butt Monkey: Batman keeps leaving him behind.
- Deadpan Snarker: "I'm chatty. It's part of my charm."
- Game-Breaking Injury: Injuring his leg while he and Batman chase after Red Hood both writes him out of the story and references him wearing a brace on his leg in the original comic.
- Sidekick Graduations Stick
Robin II/Jason Todd (Vincent Martella)
- Anti-Hero: Flashbacks show him more willing to injure people than Batman would like.
- Dead Sidekick
- Posthumous Character: The story opens with his murder by the Joker.
- Time-Shifted Actor: Young Jason is voiced by Alexander Martella. And then there's Jason as Red Hood.
Alfred Pennyworth (Jim Piddock)
Red Hood/Jason Todd (Jensen Ackles)
- Anti-Villain: Type II with shades of Type III.
- Asskicking Equals Authority: He sets himself up as the drug kingpin in Gotham and rival to Black Mask by showing up at a meeting of the heads of the organizations under Mask's thumb and giving them a bag full of the heads of their lieutenants.Hood: I did that in two hours. Wanna see what I can do with a whole evening?
- Back from the Dead
- Big Bad Ensemble: With the Joker.
- Came Back Wrong: According to Ra's Al Ghul.
- The Chessmaster
- Cold Sniper: He kills three people from a significant distance without touching Batman or Nightwing standing to either side of them.
- Combat Pragmatist: Though he has plenty of gadgets up his sleeves, he's not afraid to use anything in the environment to his advantage in a fight, and, as noted under The Unfettered, is willing to use any methods necessary to gain an advantage.
- Complexity Addiction: His ultimate plan to get Batman to realize that sometimes Murder Is the Best Solution, specifically regarding irredeemable people like The Joker. Instead of just finishing The Joker off himself and using that as the ultimate symbol of his new pragmatic approach being more effective than Batman's idealistic approach, he goes to extra trouble to get Batman to abandon his Thou Shalt Not Kill rule and finish The Joker off.
- Cool Helmet: It explodes too.
- Crazy-Prepared: To be expected, as a member of the Bat Family.
- Deadpan Snarker
- Faux Affably Evil: He has a rather snarky, personable, and somewhat laid back personality, is cool under fire and logical with always a few tricks up his sleeves and sharp tongue to match. However, this belies his true nature of an angry, violent, young man with serious emotional and (possible) mental issues.
- Guns Akimbo: Likes to to use a pair of handguns.
- He Who Fights Monsters
- The Heavy: While he is the main antagonist and the one who drives the plot along for the majority of the film. It is The Joker who caused all that happened in the film to come about.
- Irony: There's the fact that his actions are based around heroism, but are so brutal that he is a firm villain. But there is also a bit of symbolic irony in a bat family member becoming a new Red Hood, with the original being the original villain alias of who else but The Joker.
- Knife Nut: After the guns, before the fists, he has a knife that can cut Batman's cables.
- Knight Templar: Believes that everything he has done, every life he has taken, has been in the name of bringing order and stability to Gotham.[To Batman] "Yes. You can't stop crime. That's what you never understood. I'm controlling it. You wanna rule them by fear. But what do you do with the ones who aren't afraid? I'm doing what you won't, I'm taking them out!"
- The Reveal: Blood analysis shows that he's Jason Todd.
- The Unfettered: Will use whatever methods necessary, no matter how brutal or violent, to achieve his end goal of "cleaning up Gotham."
- Woobie, Destroyer of Worlds:He was once the partner of Batman and a former juvenile delinquent whose hard background, brutal death at the hands of the Joker, and subsequent resurrection drove him over the edge to become the murderous Knight Templar Red Hood.
- Would Not Hurt A Child: Threaten to kill any of the drug lords if they sold drugs to children.
Joker (John DiMaggio)
- Ax-Crazy: As usual. The movie opens with him beating Jason Todd with a crowbar. Almost to death. Then the bombs finish it.
- Big Bad Ensemble: With Red Hood
- Evil Sounds Deep/Guttural Growler: Highly unusual for the Joker, but this plays into his Darker and Edgier characterization.
- Evil Is Hammy/Large Ham: This aspect of him hasn't changed.
- The Heavy: This entire movie is entirely his fault, and he doesn't feel a drop of remorse for it.
- Monster Clown: Moreso than other incarnations.
- Would Hurt a Child: His first scene shows the Joker beating a teenage boy nearly to death.
Black Mask (Wade Williams)
- Bad Boss: When ranting about Red Hood, he's willing to randomly punch his subordinates.
- Big Bad Wannabe
- Captain Obvious: During a rant about taking down the Red Hood.Mask: Maybe my last request was too mired in subtlety. I want this man dead. When I say "dead", I mean "seriously dead". Beaten. Broken! HIS-HEAD-MOUNTED-ON-MY-WALL KINDA DEAD!Ms. Li: Understood. We'll be taking further precautions at every transaction.Mask: Screw that! Time he learned that this is a contact sport. We're going on offense. Rough up his business; something big! Something loud! When he shows up to shut us down, have a party waiting for him! And when I say "party" I actually mean a 'whole lot of people who are gonna KILL HIM!Li: I figured.Mask: Just being clear.
- Even Evil Has Standards: He's clearly uncomfortable with hiring the Joker.
- Hair-Trigger Temper: Prone to beating up his subordinates for little to no reason. Though, to his credit, he is less homicidal than the comic version.
- Large Ham: His mannerisms are based on Al Pacino in full overblown gangster mode.
- Unwitting Pawn: Of the Red Hood.
Ms. Li (Kelly Hu)
- Deadpan Snarker: Emphasis on deadpan.
- The Dragon: To Black Mask. She doesn't do a lot onscreen, but she's apparently the one who organizes Black Mask's heists and plans out said heist's finer details.
- Gender Flip: Mr. Li in the comic.
- Number Two: To Black Mask.
- The Stoic
- Not So Stoic: She loses her composure while the Joker is holding her, Black Mask, and several drug dealers hostage, and is preparing to set them on fire.
Ra's al Ghul (Jason Isaacs)
- Affably Evil: He states that he never resorts to violence or killing unless it's for a "greater cause" and is deeply remorseful that his hiring of the Joker was ultimately what lead to Jason's murder; his attempt to fix things via Lazarus Pit resurrection is what sets off the main plot.
- The Atoner: After Jason's death, Ra's chooses not to oppose Batman again, and tries to resurrect Jason to try and repair the damage he inadvertantly caused.
- Beard of Evil
- Even Evil Has Standards: He is greatly upset by the death of Jason, calling it both unnecessary and brutal. Batman points out he never had a problem killing before. Ra's counters by pointing out he has killed for a greater good, never for laughs as Joker did.
- My God, What Have I Done?: After his actions cause Jason's death, and again, after hearing of Jason's actions as the Red Hood, following Ra's raising him from the dead.
- Unwitting Instigator of Doom: Twice. First, when his hiring of the Joker was ultimately what lead to Jason's murder. Second, when he did try to resurrect Jason to try and repair the damage he inadvertantly caused, but his actions only made things worse.