Defrosting Ice Queen/Sugar and Ice Personality: Penny, while she certainly likes male attention, has trouble early on with actual intimacy. By the end of the comic, she turns a shouting match with Aggie into violently passionate rage sex that demolishes a room and most of the girls' clothes.
Sometimes doing the right thing hurts. But that's when you have to.
The Aloner: Aggie nearly ends up as this in a dream.
Ambiguously Gay: (not only does Karen assume she's gay for a full year, and Lisa briefly consider Sara's theory of attraction between her and Penny, but as of "There Are No Rules" Aggie herself now seriously thinks she may be gay. As of the Distant Finale she is shown wearing a male outfit and is said to be dating girls exclusively.
Cool People Rebel Against Authority/Former Teen Rebel: Aggie actively cultivates a rebellious attitude in memory of her former hippie mother. As she matures, she learns there are other ways to speak truth to power than through protesting and modern art. She ultimately becomes a lawyer.
Beautiful All Along: Played with. Karen might've gotten pretty on the outside, but it didn't help her self-esteem, improve her outlook, or make her a better person; it just made her believe that image was everything.
"Not If They Enjoyed It" Rationalization: She gives Marshall involuntary manual stimulation in the shower, and takes his physiological reaction as consent, ignoring his clear discomfort before and after.
Saintly Church (a one-person embodiment and the diametric opposite of Charlotte's intolerant, near-psychotic fundamentalist nature, although sometimes her religiosity can be a source of angst as well as friction with others)
Wise Beyond Their Years (a teenage example of the trope, she's easily the most mature character apart from Aggie's father Nick)
Brandi Jones, Penny's friend and Stan's girlfriend
I understand boys. Boys show what they feel. Boys' friends are their friends. Girls are the ones to watch out for.
Future Loser/Jaded Washout: Heavily implied to be in her forseeable future, at her graduation, she is seen staring at her diploma as if she does not know what to do with it. Word of God says that by the time Quiltbag takes place, she is a sophomore in college who "still thinks she can turn this thing around," and by the time of her five year reunion, she is "disappointed." (And apparently not in attendance.)
Know When to Fold 'Em/Opt Out: During "The Popsicle War", Meg makes a large show of declaring that she is done with the petty popularity games of high school. The manner in which she does this makes it clear that Meg believes that opting out is akin to declaring a victory over her opponents.
Attention Whore: Downplayed eventually, in a very literal fashion. Helen desperately wants acceptance, approval, and attention, no matter the source; she believes that letting herself be used is the only way to achieve these ends. This culminates in a Heroic BSOD/Villainous Breakdown when she realizes she slept with resident skeezoids Bob and Elmer while under the influence of alcohol.
Driven by Envy/The Resenter: Helen drifted apart from Penny's group of friends in middle school, and resents them for excluding her from their clique. She is implied to have been envious of Penny's social status in childhood, as well.
Extreme Doormat (She wants people to use her and is at a loss whenever no one needs to.)
I Just Want to Be Loved/I Just Want to Have Friends: Very downplayed; after remembering her rape and overhearing Brandi and Stan's healthy sexual relationship, Helen realizes she has heretofore been unable to differentiate between being loved and getting screwed, and has a breakdown.
The Friend Nobody Likes: Believed herself to be this in Penny's clique; was this in Charlotte's and Karen's clique, and Helen's belief that she was this to Aggie's clique led to Helen herself sabotaging their friendship with her.
Defrosting Ice Queen: Double Subverted. She's introduced as nutty, cold and eager to "punish" whomever she thought deserved it, but her interactions with Duane showed her getting mellower... Until The Stinger of the second chapter of the Missing Person arc reveals that she is the one who kidnapped and attempted to murder Cyndi. Her interactions with Duane, however, are what inspire her to reform while in prison.
The Fundamentalist (big time, but after befriending Duane she becomes gentler and more open-minded... or so it seems, until she kidnaps and tortures Cyndi in what she believes is God's punishment for Cyndi's Manipulative Bastard actions)