Voiced by: Scotty Bakula
An orange male cat with dreams of becoming a Hollywood star.
Voiced by: Jasmine Guy (speaking), Natalie Cole (singing)
A white female cat who works as a secretary for the Animal Actors' Agency. She originally came to the Hollywood business to become an actress prior to the movie. Despite her singing voice, she was not accepted as an actress.
Voiced by: Kathy Najimy
A hyperactive hippo who tends to look at the bright side of things.
Voiced by: Hal Holbrook
A cynical goat.
Voiced by: Donnie Knotts (speaking), Rick Logan (singing)
A nervous turtle who is always seen reading his fortune cookies.
Voiced by: Betty Lou Gerson
A female fish who is a dancer and a close friend (or maybe more?)
Voiced by: John Rhys-Davies
An elephant who is making a living being the logo for Mammoth Studios. He is also a skilled pianist and composer.
Voiced by: Matthew Herried
A young penguin who meets Danny and gets involved in his aspirations.
Voiced by: Rene Auberjonois
Voiced by: Ashley Peldon (speaking), Lindsay Ridgeway (singing)
A child star and shining jewel of Mammoth Studios. To the general public, she is "America's Sweetheart and Lover of Animals". In reality, Darla's is a self centered actress not willing to share the limelight with anyone else.
- Big Bad
- Bitch in Sheep's Clothing: She acts all friendly in the eyes of the general public. In reality, she is extremely mean and self-centered.
- Blondes are Evil: She has blonde curly hair and definitely evil.
- Enfant Terrible: She is an extremely bratty little girl off screen.
- Evil Laugh
- The Fake Cutie: She acts like a cutie on screen and to the general public but in reality, she is not.
- Fatal Flaw: Her temper and her ego.
- Hair-Trigger Temper: She keeps it under wraps usually.
- Huge Guy, Tiny Girl: Non-romantic: Darla is the Tiny Girl to Max's Huge Guy.
- It's All About Me: Pretty obvious.
- Jabba Table Manners: When Darla pigs out on a huge pile of junk food — and one apple — in her balcony box.
- Laser-Guided Karma: After nearly trying to get the animals fired, she herself is put out of a job and is seen grumpily working as a janitor in the end credits.
- Kids Are Cruel: She is an extremely cruel kid.
- No Celebrities Were Harmed: Darla's looks are obviously based on◊ Shirley Temple◊ with some Jane Withers thrown in, and her first name is taken from another child star.
- For a bonus, most of Darla's characterization is inspired by a Shirley Temple rumor from back in the day... that instead of being a sweet little girl, she was really a 30-year-old chain-smoking midget who hated children and animals. This is false by the way.
- Nice Job Fixing It, Villain: Darla's attempts to stop the animal's performance result in one of the most spectacular auditions in all cinema history, complete with gunfire and spectacular light performances.
- Nice Character, Mean Actor: On screen, Darla is the sweetest and cutest little girl with a love for animals you'll ever meet. Off screen, she's a self centered prima donna that can't stand animals.
- Oh Crap: After she realized that she blurted out through the whole crowd in the theater.
- Princesses Prefer Pink: Not that she's official royalty, but Darla is surrounded by sugar-pink and purple and hearts and cuddly wuddly stuffed animals to project an image of innocent cuteness.
- Red Eyes, Take Warning: Her pupils turn red when she's extremely upset.
- Regal Ringlets: Darla wears them.
- Sitting Sexy on a Piano: During Darla's "Big and Loud" number — since it's a Max-sized piano and she's small, she can roll around for several minutes with no danger of falling off.
- Spoiled Brat: She's the main star of Mammoth studios, has a butler, a huge home and lots of stuff, and is very much a brat.
- Squashed Flat: Darla gets this treatment in the climax.
- Stepford Smiler
- Tastes Like Diabetes: In-Universe, Darla is known as an extremely cutesy character on screen and to the general public.
- Twitchy Eye: Darla sometimes does this when she's angry (or trying to hold back her anger).
- The Vamp: Oddly and disturbingly enough.
- Villainous Breakdown: When all her efforts to stop the animals impromptu number in the finale backfire immensely. It ends with her outing herself to an audience while she's ranting at Danny.
- Villain with Good Publicity: Darla gets away with most of the crap she inflicts on everyone around her because she is the star of Mammoth studios and puts up an extremely deceptive cutesy image before the general public.
Voiced by: Mark Dindal
Darla's Frankenstein monster-like butler. He provides the muscle for Darla throughout and is generally part of the reason no one stands up to her.
- Ambiguously Human: He looks like a cross between Frankenstein and King Kong.
- Bald of Evil
- Battle Butler: He is a gigantic butler who does most of Darla's physical work such as hurting animals who upstage her.
- The Brute / The Dragon: To Darla.
- Catch Phrase: "YEEEEEEEES MEEESS DEEM-POOOOOLLLL..."
- The Comically Serious
- Evil Sounds Deep
- Hoist by His Own Petard: During the climax, he tries to pull the balloon of Darla toward Danny to finish him only to realize he pulled it right in front of a sharp part of the building. Cue Oh Crap as Danny gives an Ironic Echo from earlier in the film and pops the balloon sending it and Max flying into the distance.
- Huge Guy, Tiny Girl: Non-romantic: Max is the Huge Guy to Darla's Tiny Girl.
- Offscreen Teleportation: When Darla is escorting Danny out of her mansion after giving him the Big and Loud musical number, Max holds a door open for them. They walk down the hall a short way, and Max is waiting for them at the exit with Danny's hat, despite him never moving from the first door. Leads to a Funny Moment when Danny stops, stares at Max, turns his head to see if he's still holding the door, then turns back and stares ahead blankly with a befuddled expression as Darla hands him his hat. Can be seen here at 4:18
- Perpetual Frowner / The Stoic: For most of the movie least till the climax where he gets a bit Axe Crazy and his Oh Crap expression when Danny has him beat.
- Stealth Hi/Bye: Max is apparently an expert in this despite his size.
Voiced by: George Kennedy
The man in charge of Mammoth Studios.
- Ironic Name: L.B. Mammoth, who turns out to be really short.