Fandom Nod: Several of the portraits in the first game's levels are based on photos of members of the (now defunct) Spookhouse forum for Nocturne.
Retroactive Recognition: BloodRayne 2 was only now-veteran voice actor Troy Baker's second video game role. The Guttural Growler voice he uses for Kagan makes it hard to spot him, but Severin is much closer to one of his "standard" voices, bar the British accent.
The Character Died with Him: Lynn Mathis (who voiced Stranger in Terminal Reality's other games and DarkMan in the first BloodRayne) died in 2003, barely a year after BloodRayne was released. Out of respect, Terminal Reality replaced DarkMan with Severin (voiced by Troy Baker).
Throw It In!: Part of the script was written in a bid to make Laura Bailey blush and the lines that did were the ones that were used.
What Could Have Been: Rayne's initial character design◊ was meant to be reminiscent of Svetlana Lupescu from Nocturne, Terminal Reality's Survival Horror game that inspired BloodRayne but was later scrapped and redesigned into the Rayne we all know today. There's a passing reference to a character that might be Svetlana in the first game, when Mynce mentions one of Rayne's half-sisters also working for Brimstone.
Creator Killer: This was the first and last time Billy Zane ever distributed a film. The box office failure combined with a lawsuit filed against him by Uwe Boll led Zane to quit the film distribution business.
Laura Bailey: Oh God, that movie sucked. And that movie was so bad. I saw it on The Movie Channel and I couldn't even get through 20 minutes of it! It was so bad and it was kinda sad that they took that because I really liked the games.
Screwed by the Lawyers: Billy Zane not only co-starred in this movie, he also co-owned Romar Entertainment, the company that distributed the film in the U.S. Romar promised Boll that the film would open in 2,000 theaters across the U.S. and ended up opening in 950 of them, which led to Boll suing Zane and Zane getting out of the film distribution business after settling.