Buster and Babs getting into a water gun fight that results in them and Byron the Basset riding a raft down the river, where they meet all sorts of dangerous, Southern animals.
Plucky Duck joining Hamton's family on their trip to Happy World Land — only to find himself in the car trip from Hell, especially with Hamton's Uncle Stinky and a deranged hitchhiker who just escaped from the prison psych ward.
Fifi La Fume pursuing her favorite movie star, Johnny Pew, in hopes of getting his autograph.
Fowlmouth dragging Shirley (who has a summer job giving psychic readings to people on the beach) to see a movie with him — the horror flick Skunknophobia — which he's seen 100 times.
Elmyra causing chaos in the search for a new kitty cat, first while on a drive-thru safari.
Other cast members show up in one-note incidents that weave in and out of the main storylines.
Tiny Toon Adventures: How I Spent My Vacation contains examples of the following tropes:
Anguished Declaration of Love: Babs attempts to give one to Buster right when it seems they're going to go over a cliff, but Buster shushes her, saying "I feel the same way."
Badbutt: Perhaps in consideration of cinema-goers' sensitivities, Fowlmouth is reduced to saying "dad-gum" instead of his usual cussing, pretty much losing the character's (admittedly one-dimensional) point for existing in the first place.
Ralph the Guard shows up to chase Plucky in the intro when he and Hamton are camping in the sporting goods store.
Fifi tries to get into the hotel Johnny is staying at, where she encounters Jay Leno, Roseanne Barr, David Letterman (who is there for one of a few reasons listed in the end credits), Ed McMahon, Johnny Carson, Barbara Walters, Oprah Winfrey, and Arsenio Hall.
Roseanne appears again later on in the movie theater, along with Jack Nicholson, Pee-Wee Herman (which, given that Paul Reubens' arrest for indecent exposure at a porno theater happened a year before this was released on VHS, makes the joke of him in an actual theater with a big bucket of popcorn on his lap, very disturbing. See Getting Crap Past the Radar below), and Cher.
At one point Babs, Buster and Byron are saved by Superman.
Chekhov's Gag: Two occur back-to-back during the mine cart chase:
The tracks lead into a tunnel at one point. Buster tells Babs to hold her breath and make a wish. The same was done by Plucky and the Pig family when they drove through another tunnel earlier in the movie. Babs gets her wish granted when the next section of the tracks goes through a mall.
Near the end of the journey through said mall, Buster and Babs discover the "new box look", which was inadvertently started by Dizzy after he had to wear a box to hide his furless body near the beginning of the movie.
The Chew Toy: Several of the characters, but especially Plucky (as usual) and Babs. In fact, the entire Buster and Babs subplot can be described as "hilarious stuff happens to Babs with the occasional reprieve."
Comedic Underwear Exposure: When a female possum's green mini-skirt falls off, she is left in her blue bloomers or pantalets. Facing the viewers, she tries to cover up with modesty and coyly giggles as she exits the scene.
Numerous one-shot villains from past episodes are in Buster and Babs' audience on the Delta Burke.
When Buster is opening his locker at the end, a photo of Rover, his pet dinosaur from the episode "Rock and Roar", can be seen on it.
Elmyra mistakes skunk-movie star Johnny Pew for a "stinky kitty," the same thing she did to Fifi - with the same results - in "Out Of Odor."
Covers Always Lie: The DVD cover shows Buster, Babs, Plucky, Hamton, Elmyra and Dizzy on a ride at Happy World Land, but in the movie only Plucky and Hamton go there and they don't even get to go on any rides.
Fur Is Clothing: Dizzy Devil is afraid to spin because he's shedding, and doesn't want to end up naked. Eventually he does spin and lose his fur, and spends the rest of the movie wearing a cardboard box and feeling ashamed for being naked.
One of the more shocking examples the part where Fowl-Mouth walks past a row of celebrity caricatures to his seat, one of which is Paul Reubens (dressed as his character Pee-Wee Herman) holding a bucket of popcorn. True, he isn't shown actually masturbating (since this is a kids' show and not even American kids' shows go that far, even if Cartoon Network is behind it), but since the film was released over a year after Paul Reubens' arrest for indecent exposure at an X-rated theater, it can be assumed that this wasn't just a "Funny Aneurysm" Moment (or maybe it was, considering how long it takes animated pieces to be made and the fact that world events can turn a once innocent joke or harmless dramatic scene into someone sleazy, disturbing, or depressing).
"Look! He's got no pants!" - The censors probably took a day off.
And after that when the Boos are introducing themselves, the eldest says "I'm Big Boo" but it sund like she's saying "I'm Big Boob" and yes, they're big, as Buster will soon find out.
Grand Finale: Technically of the original series if you don't count the specials.
Hammered into the Ground: Happens to Mr. Hitcher when he is dropped off and tries to attack Plucky and the oblivious pigs, accidentally cutting down a telephone pole that lands on him.
Horrible Judge of Character: Hamton and his family, who can't seem to realize that Mr. Hitcher is probably a dangerous criminal, even with the chainsaw and hockey mask.
Idiot Ball: Hamton and Plucky both hold one in the comic book scene. Hamton, who gets carsick when he reads in the car, refuses to take his eyes off Pluckyís priceless comic book, and Plucky does everything BUT get the comic book out of the line of fire.
Also the fact that Hamton didn't TELL him he gets carsick when reading until AFTER it happens. His mother warns Plucky but doesn't outright state it.
Kissing The Ground: Plucky is so happy to be home from a horrible road trip that took all summer that he kisses Hamton's lawn, only to discover that there's fertilizer on it as plants start to grow on his tongue.
Medium Awareness: There are quite a few references to this movie being on video.
Potty Emergency: Defied when Plucky goes to drink some water at a gas station and Hamton's mom stops him because if Plucky drinks all that water, they're going to have to put up with him begging to go to the bathroom.
Shaggy Dog Story: Plunky manages to endure the whole road trip as he and Hamton's family finally reach "Happy World Land" But after a very brief tour of the park and the rides from a monorail, the family promptly files back into the car to drive home. Why? If they went on all of the rides, they'd have nothing to look forward to next year.
The THX logo is parodied as THUD, very cleverly. Just about everyone in the theater covers their ears as the trailer begins, knowing to expect loudness. Then, they get blasted out of their seats as a sound similar to the THX Deep Note plays. There is also text below the THUD logo saying "A Division of Mucasfim," a reference to Lucasfilm, which used to own THX. At the end, an announcer (who some people think sounds like Alan Rickman) says "The Audience Is Now Deaf," spoofing THX's motto "The Audience is Listening."
The entire possum plot, complete with the bridge shot and "Dueling Banjos" scene is a PG-rated version of Deliverance.
After Uncle Stinky exhales on Plucky, blowing the color off of him, and Hamton repaints him:
The raft ride is a reference to Huckleberry Finn, with Babs and Buster having encounters with Southern stereotypes, enjoying the river scenes, and Babs referencing Huck Fin at the end of the ride.
Slapstick Knows no Gender: Par for the series, but really exemplified here: Babs probably matches Plucky in having the most slapstick in the movie, Fifi gets her own sequence where she's smashed a few times, as does Elmyra. In fact, the only characters at all who don't get any slapstick are Hampton and Shirley - which is more because of their roles in the plot than anything else.