Bubblegum Crisis: Tokyo 2040 spent its first half building the Sempai Kohai relationship — and Romantic Two-Girl Friendship — between Priss and Linna. The final half of the series drops this subplot like a hot potato for a sudden romance between Priss and Leon that fits the "offscreen explanation" part of the trope to a T; Leon shows up at her club while she's on stage, does a smarmy clap for her, Priss grins a bit, and from thereon in they're treated as dating. Ironically, it was the original Bubblegum CrisisOVA that gave rise to the mantra among many early anime fans that "All women in anime are lesbians. Unless they're bisexual."
At its core, the interesting relationship between the two titular Nanas: one a tough punk-rocker, the other a girly girl who likes frilly fashion. Disappointingly, most of the manga depicts the two girls' relationships with comparatively bland or annoying guys, to the point where the carefully set up romantic tension between them is all but thrown overboard—which isn't helped by the fact that one of them gets knocked up.
One Nana develops an infatuation with her roommate later in the series, encouraging her to get together with (and get back to) the one decent opposite-sex relationship that she finds... because the man in question is close to both of them, and that will keep them together. She moons over her in a way that she doesn't moon over the man she eventually marries. Nothing comes of it though and both women do eventually marry. In later chapters however, we find that, after a short while, both women do separate from their husbands. One of them leaves Japan, and the other plans to go after her, claiming that she "loves" her.
The OVA for Saber Marionette J introduced Marine, a new Marionette who somehow imprinted on Lime as her Master — and therefore, her implicit love interest. Although there is plenty of subtext in their relationship, Marine eventually realizes that her true love master was the ocean, the first thing she saw when she awoke (underwater). Complicating this is Lime's perception of affection is generally much more childlike than the other androids. The more likely explanation is the OVAs are Non Serial Movies whose topics couldn't be put into the next season of the show.
The opening credits of Venus Versus Virus combine this with Bait-and-Switch Credits, although the two girls never really have that kind of relationship in the show, which isn't helped by the Gecko Ending of the anime. Despite all this the manga is licensed as a Girls Love manga. The two have quite the Romantic Two-Girl Friendship - not helped by all the touching, blushing, and lampshades- and by the end it's all but stated Lucia is in love with Sumire. However Sumire's bond with Lucia took a turn for the worse late into the series and even when it's rekindled it's ambiguous whether her affections are the same
On the other hand, Utena has a rare male/male version of this trope in the relationship between Mikage and Mamiya. While their initial interactions are unambiguously romantic, it eventually turns out that Mikage (or better said, Professor Nemuro) was actually in love with Mamiya's older sister Tokiko all along, with his desire to help Mamiya being primarily for Tokiko's sake. Furthermore, the Mamiya seen throughout this story arc is revealed to actually be an illusion acted out by Anthy; the real Mamiya was Dead All Along, and the entire "relationship" was nothing more than a ploy by Anthy's brother Akio to manipulate Mikage.
Nevertheless, the Megami Sound Stages tried to explain that really, Fate was "more of a sister to Vivio" than a mama, and that Fate sent herself into deep space missions in order to "get out of the way." Something the voice actresses of the characters themselves have declared Canon Discontinuity.
The Vivid manga (which take place two years before Force, in the same continuity as the other works in the franchise) retconned the Megami Sound Stages, by the notes along the first chapter (which said Fate and Nanoha raised Vivio together) AND Vivio, who said in the same first chapter that she had two mothers (and acknowledging it's something strange, but they're her mothers), and the last Soundstages released for the C76 Comiket definitely sunk any possibility of the Yuunoha pairing. And in bonus, we have Vivio in this Sound Stage saying in the prologue that she is Nanoha and Fate's child.
The opening credits of Magical Pokaan feature TWO pairs. Although the four main female characters are paired up and almost-kiss each other while sort of naked, nothing even close to this happens in the actual show... but then again the entire OP seems deliberately misleading.
Used in an episode of Rental Magica. In order to ensure the safety of her mage corporation, Addie plans on getting married - to Honami. Almost the whole episode is spent on them preparing for this girl-girl wedding. Then Itsuki interrupts, runs off with the brides, and declares that he'd rather marry them both. It was an Almost Kiss too...
A lot of relationships between the girls in Oniisama e... (which is a manga of The Seventies) get blown up prematurely by various degrees of human failings. In the end all female main characters - with the exception of Rei, who is dead by this point - enter relationships with men.
Actually, Nanako refers to the "university student" she fancies in the end... with gender-neutral pronouns. She could perfectly be about to date an university girl, as far as we know.
Hidamari Sketch has a mild example, in that Miyako sometimes flirts heavily with Yuno, just to let things fizzle out because of her unfocused nature. And then of course there is the relationship between Hiro and Sae, although that is more a clear case of Romantic Two-Girl Friendship.
The relationship between Mikan and Hotaru in Gakuen Alice has something of this. Despite both girls becoming ever closer in the course of the series, leading to numerous heartwarming moments, it is becoming very clear that the writer intends to hook Mikan up with Natsume, a boy. Sure, all of the characters are relatively young, but still...
The Aoi Hana anime combines this with Bait-and-Switch Credits, what with all the romantic imagery involving Fumi and Akira. Sure, Fumi is openly lesbian and seen in an unambiguous relationship with a girl, but that girl is not Akira.
The manga also seems to moving into this territory. Of all the (potential) yuri pairings, only the one between Orie and Hinako seems to have survived the author's penchant for odd plot twists.
The emotions between the girls in ARIA often reach romantic levels, only to be cut off before they become too obvious.
Fans noticed quite a few scenes in which Alicia and Akari seem to be very close to kissing. Maybe this is the reason why Amano introduced some rather forced male romantic interests later on.
A somewhat mild version occurs in Magical Project S between Sasami and Misao, since the characters are underage. They're very close friends, and there's subtext there, but the end of the show very haphazardly pairs them up with their Ryo-ohki and Rumiya respectively, even though they didn't know either boy had human forms until the end of the show.
Youre Under Arrest features a very intimate relationship between two Lovely Angels. The couple gets a lot of Ship Tease from manga omakes, endings of the anime, and official artwork. However, they both have male love interests and don't express any romantic feelings for each other. Compared to their male love interests, which tend to be forced and they typically have an awkward relationship with, their relationship is the main cause of troubles in the series.
Rika and Hanyuu come millimeters away from kissing -with hands intertwined and eyes closed, no less- in the closing theme of Higurashi: When They Cry: Kai. Rika is fairly Ambiguously Bi but it's never so obvious.
After all the heightened emotions, almost-declarations of love and subtext between them, Canaan and Maria choose to separate because their lifestyles are too different—and they don't even once contact each other again. A lot of yuri fans felt this was quite a cop-out. At least the makers decided not to kill any of them off or suddenly give one of them a straight option out of nowhere...
Hyouka has a lot of implied romance between Chitanda and Ibara in the endingcredits. Strangely, this isn't even shown until the end of the second episode, and by then it's pretty clear that they don't even have a Romantic Two-Girl Friendship in the series itself.
Minako and Saki in Erica Sakurazawa's Between The Sheets. Not only does Saki vehemently reject Minako and even denounce the friendship at the end after a series of events, but Minako ends up with a new boyfriend. (Though she does say she'll never love him as much as she loves Saki.)
A common complaint about Kissing Jessica Stein, in that the two women don't end up together (and it's implied that the Jessica ends up with her ex-boyfriend shortly after the two come out). The problem with this, though, is that people don't seem to note that it's outright stated that neither woman is explicitly gay—in fact, outside of the relationship, Jessica is only seen dating guys while Helen dates both. Also, the film shows the entire course of the relationship, over the space of nine months.
In Twilight (the movie) Alice says that she and Bella will be "really good friends" in a suggestive manner, and Edward responds with a sneering, "Can you keep your thoughts to yourself!" It turns out that Alice is SO TOTALLY NOT GAY, and she REALLY REALLY MEANT THEY WOULD JUST BE GOOD FRIENDS.
The book has a good bit of this at well. That line is in the actual book, as is Alice pointing out that Bella" does smell good!" As is the bit about Alice loving Bella deeply. Then throw in how often Alice hangs on Bella, carries her around, etc. and you've got a ripe lesyay shipping bed—except Meyer, as a devout Mormon, would not write lesbians into her story.
On the DVD commentary for Bring It On, the director notes that a scene showing Kirsten Dunst and Eliza Dushku (who has a strong lesbian fanbase) in bed together wasn't intended to hint at girl-on-girl action. Really!
The Israeli movie Walk on Water is about a Mossad agent who is sent to assassinate an aging former Nazi, then meets the man's grandson, who gives him a new outlook on life. They get really close. I mean, really close, and then it is revealed that said grandson is gay. Then, in the end, the Mossad agent gets together with the grandson's sister, an until-then-minor character. The Ho Yay between the agent and the grandson, however, was so blatant that I couldn't help but wonder if the grandson was originally intended to be the agent's Love Interest.
Considering that the film is all about people of different categories looking past their differences, I think it's actually thematically necessary the Mossad agent to be straight. But I would agree that the homoeroticism between the two leads is thick, and it's easy to see him marrying the sister as a thin substitution for his real goal.
In the Female Prisoner Scorpion series, protagonist Matsu's relationship with Yuki is pretty ambiguous. In the first film, they clearly care about each other a lot, but it's not clear if this has a sexual dimension. The third film features the same actress playing someone with the same name, but who is clearly a different character, although Matsu apparently recognises her (it's a tad complicated). This Yuki is either enfatuated or fascinated with Matsu, but is fairly Messed upwhen it comesto sexual matters.
A rather old example, from a 1930s play-gone-movie. Karen and Martha from The Childrens Hour share a close intimate friendship, though Karen is engaged to a man. Their friendship is full of subtext on both sides but only Martha is revealed to be gay.
The trailers for Jennifers Body tended to focus heavily on the Les Yay kiss between Jennifer and Needy. In the actual movie, Needy is completely straight and Jennifer is a literal man eater. It is possible that the kiss was shoehorned into the film explicitly for trailer material. That said, Jennifer's true orientation is left ambiguous. It's possible that she dates a lot of men simply because of social-jockeying and indeed, some of her lines hint that she's bisexual or a closeted lesbian. Given that she frequently interrupts intimate moments between Needy and Chip and is incredibly possessive towards her, there is a lot of evidence to support the latter theory.
Quite a few Alias fans suspect that Rachel was intended to be a lesbian due to her pointedly saying that Agent Grace wasn't her type in an early appearance. For whatever reason, she was confirmed as straight later.
The majority of the advertising for Rizzoli & Isles seems to be created with the mandate "do everything you can short of blatantly lying to convince prospective viewers that the leads could end up making out at any moment." Anyone actually tuning into the show will discover that, despite copious amounts of Les Yay, they are both, in fact, heterosexual.
Pop group Tatu did this for early marketing. Horny guys think the two singers are lesbians, buy the CDs, find out they're a pretty good group. Continued onstage after they were known not to be, sort of an odd musical equivalent of Kayfabe.
Katy Perry's "I Kissed A Girl", which is pretty unambiguous in its title and rode to success on being a mainstream song about girl-on-girl action, backs away from the idea in the video by not showing any girls kissing each other and finally turning out to be Katy's dream as she wakes up beside her boyfriend. Her boyfriend, you see? Okay.
The fact that she has a boyfriend is stated in the lyrics, so it's not too much of a switch.
There was a spoof of the song featuring Karen Gillan as Katy Perry called "Why I Kissed a Girl" where she claims she only kissed a girl to get a hit. At the end of the video she is picked up by her boyfriend and rudely ditches the female dancers she was performing with up to that point.
Elphaba and Glinda from Wicked have a very close bond. It starts off as pure Foe Yay full of Belligerent Sexual Tension but morphs into a fluffy friendship, most likely the closest in the musical. It's even thicker than in the source book, as the musical focuses almost entirely on them. However Glinda is apparently in love with Fiyero and Elphaba's in love with Fiyero, plus he lives in the musical, so that gets in the way of any of their romance. Word of Gay backs their romance up but it's hard to say whether they were ever together.
Since your typical hentai game has one or two major male characters and boatloads of pretty girls, the marketing images often involve multiple female characters looking mighty friendly, even if there's not even a romantic friendship between them in-game. For that matter, several games play up a romantic friendship between two girls and teasingly suggest that one of them may be gay for the other, only to eventually drop that subject without so much as a girl/girl kiss ever being shown. Obviously some authors do not agree that Girl on Girl Is Hot. Or are using Girl on Girl Is Hot to advertise.
Tohsaka appears to be bisexual in one of the hentai scenes in the Fate route of Fate/stay night, but this is never mentioned or addressed outside of that particular scene.