Ragtag Bunch of Misfits: Can you say any of the farm animals are considered 'normal'? You got a pig whose imagination can come to life and take others with him. A loud-mouth rooster that likes to play pranks. A wacky duck that's afraid of nearly everything. A sheep who's a grumpy Cute Bruiser and her brother who's ditzy (a Genius Ditz in the cartoon). A chick who chases after a worm, coyote-road runner style. An intelligent chick who lives in his egg. A puppy who thinks he's a ferocious dog. Even Blue the cat is strange in her own right. Her bio said she's mystic and mysterious.
Two Girls to a Team: Abrasive Lanolin Sheep and kind, intelligent Blue the cat. Though that was once Blue came into the picture... and before she was Chucked. Could've been subverted, had they decided to add in Jodie the horse (look up the trivia section).
Childhood Friend Romance: In the cartoon "Stark Raven Made", Orson was in love (and still is to this day) with a pig girl name Lenore, but then at the age of four, Lenore had to move away and couldn't see Orson anymore which left him brokenhearted (even to this day).
Hypocrite/Hypocritical Humor: There are moments when he gets like that. For example in the episode, "The Impractical Joker", when the other animals complain to him about Roy insulting him with a joke book, Orson tells them to just ignore it, they're harmless. But when Roy does it to Orson, he fires him from the farm. The other animals (even Wade) thought firing Roy was too far.
Imagine Spotting: Orson's imagination is so powerful the other characters somehow get transported into his fantasies.
What the Hell, Hero?: There are times when he does means well and at least regrets his mistakes afterwards and sometimes tries to fix it. Then there are times when he's this trope. One example is in "Goody-go-Round" when Orson found nearly everyone was fighting and insulting each other. Orson asked what was wrong, but instead of letting them answer, he just goes and insults them, even Sheldon (who wasn't even involved in the fight!). Uh, what? He's especially this in the comic version.
Do Not Call Me Paul: Averted in the strips. Roy hates when Lanolin calls him "Bird" instead of calling him by name. In this strip, in response to Roy asking her to call him by name, she called him "Ray". He was upset.
Evil Roy: Subverted at least in the cartoon. He may be mischievous and delights in pranking the other farm animals, but he still does what he can to help them out.
Flanderization: In the comics, his appearances of early 1986 had him with a much more mellowed down Jerkass tendency and his prankster aspect merely started out with waking up other farm animals with his horn. Which eventually extended to pulling pranks just for mean-spirited fun. By 1987 his Jerkass tendencies were already taken Up to Eleven.
Not a Morning Person: He gets up to do his job, waking people up, usually with his horn... then goes back to sleep. And besides a love/hate relationship with Wade, he also has one with his alarm clock. In some episodes of the cartoon, Roy simply has a recorded version of the wake-up call to play so he won't even have to wake up.
Tsundere: (Non-physically abusive Type A) He's mainly this to Wade. he's shown deredere moments to him even if he wouldn't admit it and Wade seems pretty well aware of it.
Vitriolic Best Buds: He's pretty much this to the cast. Roy's definitely this with Wade, especially in the cartoon version. He's always making pranks to scare Wade, though he's never usually physically abusive of him (and Wade was starting to catch on to him in the later seasons) and he really does care about Wade in his own "Roy Rooster" way. Other times, they're arguing and there are other times when they actually act like friends. They had many love/hate friendship moments that leads to funny moments. Over the seasons, their relationship evolved to the point where they had the closest friendship with each other, besides with Orson.
Fluffy Tamer: Believe it or not! In "Show Stoppers", he has a big fearsome looking bull who he calls Fido, who ended up chasing Orson's mean brothers away.
Kindhearted Simpleton: Although cartoon Wade's not very smart, he is shown to be good-hearted. The difference between him and and both versions of Bo was that cartoon Bo was a Genius Ditz and Comic strip Bo was a ditz, while both versions of Wade were near Too Dumb to Live level.
Too Dumb to Live: Happens a lot to him in the cartoon version. Once, in "Snow Wade and the 77 dwarves", he ate an apple even when he was told it was poison.
Tsundere: Type B with Roy, mainly in the cartoon version. He's nice and friendly with the rest of his friends. Arguing/competing against Roy is surprisingly the only thing(s) Wade's not usually afraid of!
Vitriolic Best Buds: With Roy, mainly in the cartoon version, to the point where over the seasons they had the closest friendship besides with Orson.
The Smurfette Principle: Most of the time. In Garfield and Friends, she's the only female who regularly appeared in either short (Nermal is male, Liz only showed up occasionally, and Penelope was only in the last three seasons). The writers were going to add in Chloe, but they forgot.
The strip had Blue, but she disappeared after awhile.
Sugar and Ice Personality: She's more Ice than 'sugar', but she does brings hints of 'sugar' moments towards Bo and (mainly in the the cartoon) Orson.
Supreme Chef: In the cartoon, she's very good at making cakes and pies.
Beware the Nice Ones: As shown in the episode "Keeping Cool". He acted carefree when Orson's brothers' tried to pick on him. But DON'T threaten/mess with his friends! He got rid of Orson's brother by having them be dragged from a bus.
Although in the cartoon he wasn't as dumb as in the strip, he did have his random moments such as in the episode "Fast Food" where he was cooking for his hungry, waiting friends, one strand of spaghetti/one pea at a time, much to their dismay.
Cats Are Mean: Averted! Blue is easily one of the friendliest and most considerate characters in the cast.
Chuck Cunningham Syndrome: Both of them disappeared in mid-1988, months before Garfield and Friends premiered. They never appeared in the cartoon, likely due to the confusion that might have arisen from them being in the same series as Garfield and Odie, who don't speak.
Although Cody appeared in a few of the U.S. Acres storybooks.
Artistic License - Biology: Real-life worms are hermaphrodites but some have specified genders. They still need to become couples in order to perpetuate the species.
Also, real worms don't have teeth.
Comically Missing the Point: It was raining and Estelle told Filbert he should do something about the hole in the roof. When he told her it was the front door, she told him he should then do something about the hole at the front door. Then he flat-out told her the front door was a hole and she said she wasn't in the mood for games.