- Accidental Innuendo: In the sequel, "We're both balls!"
- Crack Pairing: Spike and Applejack's relationship. This is even lamphaded in the sequel.
- Ensemble Dark Horse: Cinnamon Roll, the adopted daughter of Applejack and Spike, is gaining quite a following for a character who isn't that integral to the story and has only a few lines of dialogue. It probably began when Ice Gaze, her VA for Daughter of Discord, posted a song parody that showed Cinnamon Roll in the bathroom at the Grand Galloping Gala singing about how she feels abandoned by her brother and how she finds it hard to fit in when everyone else in her brother's circle is older than her. DF praised her video for adding so much more to Cinnamon Roll's character than she could've thought of. This probably contributed to her getting more development in "CMC: The Next Generation." What's more, "Cinnamon Roll In the Bathroom" would eventually become Ascended Fanon when DF decided to adapt it into an animatic to be shown at BronyCon 2018.
- Friendly Fandoms: With the smaller audio drama The Apprentice, the Student, and the Charlatan. Given that cast member truesailorcomet is putting it on, and several cast members and artists from DOD appear in it, not to mention DisneyFanatic promoting it with a blind review, it was inevitable that the two works would grow in popularity within the same circle of fans.
- Hilarious in Hindsight:
- "The last chapter mentions Shining Armor and Cadence having an alicorn daughter (named Flutterby Lily). Then in season 6, we got Flurry Heart. In fact, the existence of Flurry Heart in the show pretty much got Flutterby Lily Exiled from Continuity in the Daughter of Discord audio drama and the fanfics in the BOD universe written after her introduction.
- In Daughter of Discord, Screwball wonders if it's possible that a changeling can change.
- And then later it turns out they can.
- In a season seven episode, we see Discord interacting with duplicates of himself for an extended period of time like he does throughout this story.
- Rainbow Dash once insults Applejack by saying Granny Smith eats pears. Then it turns out, Applejack herself is half-Pear.
- Jerk Ass Woobie: Possibly Discord, after hearing his origin story. He's still a malicious prankster lacking in empathy, but the origin paints him as lonely and that he was more of a class-clown than a Mad God.
- My Real Daddy: Not the story itself, but the character of Cinnamon Roll, though technically created by DF, didn't start to get much attention until Ice Gaze, her voice actor, made "Cinnamon Roll in the Bathroom," taking a character who had very little focus and not much that was notable aside from being the younger sister of one of Screwball's close friends, and fleshing her out in the span of around five minutes. If not for that video, DF might have never given the character more focus in CMC: The Next Generation.
- The Woobie: Fluttershy. On top of her regular Shrinking Violet status and being bullied in school, she chooses to perform a Heroic Sacrifice by giving up her freedom and everyone she cares about to be Discord's bride so he will not hurt anypony else. Though they do eventually fall in love, Fluttershy's initial stay with Discord, is... depressing to say the least.
YMMV / Bride of Discord