- JoJo's Bizarre Adventure: Stone Ocean: Green Dolphin Street Prison equips cuff links to prisoners on-duty off the premises that explode if they distance themselves too far from the instructor in control of them. Said cuff links are called Like a Virgin, named after Madonna's album of the same name.
- The Professional: Mathilda does a Madonna impersonation. Leon doesn't recognize it.
- Reservoir Dogs opens with some of the gang members discussing "Like a Virgin" and what it means.
- Running on Empty: Mr. Phillips, Daniel's music teacher, shows how he's a Cool Teacher on his first day of class, when he plays an excerpt of "Lucky Star", which most of the class claps and dances to.
- Working Girl: Cyn tries to dissuade Tess from impersonating her boss (which Tess says she can do) by pointing out, "Sometimes, I sing and dance around the house in my underwear. Doesn't make me Madonna. Never will."
- 13 Going on 30: In her adult life as a successful magazine editor, Jenna is shocked to learn that she knows Madonna, who gave her a signed picture.
- Planet Earth Is Blue: Margot, a girl from Nova's special ed class, is a fan of Madonna.
- Juniper Sawfeather: Haley's mom's ringtone for her is "Material Girl."
- In Beautiful Music for Ugly Children, Gabe plays B-sides on his radio show, including "Into the Groove."
- In the Small Wonder episode "Vicki for the Defense", during Joan's courtroom simulation at school, Reggie tries to bribe judge Jamie with two tickets to a Madonna concert.
- Young Sheldon: In "Blonde Ambition and the Concept of Zero", Mandy tells Missy that Madonna's not a natural blonde. Missy's mind is blown.
Music
- As quoted atop her page, Robbie Williams' "She's Madonna", which is based on Guy Ritchie's actual words when ditching his girlfriend (who also dated Robbie at one point) to hook up with Madonna.
- Australian rock band The Androids released a song in 2002 called "Do It With Madonna", a tongue-in-cheek song which plays up Madonna as the ideal woman, but also takes some lighthearted jabs at her successors. Notably, the music video shows all of the named pop stars played by some obvious drag queens, with Madonna being portrayed by a very fat drag queen for a laugh.
- "Madonna" by Drake, in which he tells a girl she could "as big as Madonna" if she associates with him.
Video Games
- Donkey Kong 64: In the game's opening theme, Lanky Kong's verse begins with the lines "He has no style, he has no grace; this Kong has a funny face," referencing the lines "They had style, they had grace; Rita Hayworth gave good face" in "Vogue".
- Doug: Both shows reference Madonna occasionally, down to Beebe's superheroine version in Quailman being named Material Girl. Not only that, she is actually the only Real Life person referenced in the show. Doug's creator Jim Jinkins appears to be a big Madonna fan.
- Family Guy: In "New Kidney in Town", after taking his first sip of Red Bull, Peter launches into a parody of the "Ray of Light" music video, complete with Rotoscoping and falsetto.
- The Simpsons:
- A Deleted Scene from "Krusty Gets Kancelled" (as seen in "The Simpsons 138th Episode Spectacular") has Krusty trying to gain viewers by promising to send them an erotic book of himself, referencing Madonna's controversial Sex book. As federal agents arrest Krusty on obscenity charges, he protests "It's not really me, I used a stunt butt".
- In "E. Pluribus Wiggum", Lenny says that Madonna was Juan Perón's wife, a reference to her role in the film version of Evita.
Other
- British ice dancers Lilah Fear & Lewis Gibson performed to a Madonna medley (although "Vogue" is the main song) for their free dance
during the 2019-2020 competitive season.
