The Melancholy of Haruhi Suzumiya: Haruhi (sanguine, despite the title), Koizumi (choleric), Kyon (melancholic), Mikuru (melancholic), and Yuki (phlegmatic). Tsuruya, an honorary member of the Brigade, also fills the purely choleric position.
Jewelpet Happiness has the boys quartet: Kousuke is phlegmatic, Takumi is melancholic, Mouri is sanguine and Taira is choleric.
Lady Jewelpet has two sets: the Petit Ladies are Momona (sanguine), Lillian (melancholic), Mizuki (choleric) and Charon (phlegmatic); the Sixth Ranger Elena is leukine. The Princes are Cayenne (leukine), Romeo (phlegmatic), Soarer (melancholic) and Levin (sanguine); the Sixth Ranger Miura is choleric.
The Legend of Zelda: The flash animations by Hadouken Dude took this to absolutely hilarious levels, with Green Link being loyal/humble, determined and moralistic/uptight (melancholic), Blue Link being analytical/nonchalant, witty and sarcastic/condescending (phlegmatic), Red Link being hot-tempered/brash, impatient and perverted/selfish (sanguine), and Purple Link being naive/greedy, idealistic and clumsy/overly dependent on others (choleric), also accused of being gay because of personality and color.
Sanguine: Yuan is extroverted, fond of wisecracks, and very protective of the people he cares about. He uses fire techniques and wears red.
Choleric: Tokito is quick to anger, perfectionistic, and impulsive. Wears shades of yellow, and fights with a very aggressive energy.
Melancholic: Hishigi is exceedingly introverted and the epitome of The Stoic, analytical and extremely task-focused. He shows textbook signs of severe depression. His color scheme is almost entirely black.
Phlegmatic: Fubuki leads the Taishiro. He is level-headed, cultured and elegant. His fighting style revolves around water and his color scheme is mainly blue.
Superbook (second season): Christopher Peepers (choleric), Joy (melancholic), Uriah (sanguine), and Gizmo (phlegmatic).
Five-temperament ensemble in Tokyo Mew Mew: At first not happy about leading, but accepting her responsibility later on, Ichigo (choleric); Minto (melancholic), Retasu (phlegmatic), the Genki Girl Bu-ling (sanguine), and Zakuro (eclectic).
Trigun more often averts this trope in favor of the Lone Hero or the Power Trio. Adding Wolfwood to the cast has a subtractive effect instead, generating a duo dynamic that leaves Meryl and Millie on the sidelines. When they participate equally they fit the four temperaments: Meryl (choleric), Vash (melancholic, though pretending to be sanguine), Wolfwood (phlegmatic), and Millie (sanguine).
In the original Weiß Kreuz Aya (choleric), Omi (melancholic), Youji (phlegmatic), and Ken (sanguine).
In Wolf's Rain: Tsume (choleric), Kiba (melancholic), Toboe (phlegmatic), and Hige (sanguine).
Your Lie in April: Arima Kousei (melancholic), Miyazono Kaori (choleric), Watari Ryota (sanguine), Sawabe Tsubaki (phlegmatic).
Yu-Gi-Oh! 5D's: Jack (choleric), Yusei (melancholic), Akiza (phlegmatic), and Crow (sanguine).