The Melancholy of Haruhi Suzumiya: Haruhi (sanguine, despite the title), Koizumi (choleric), Kyon (melancholic), Mikuru (melancholic), and Yuki (phlegmatic). Tsuruya, an honorary member of the Brigade, also fills the purely choleric position.
Jewelpet Happiness has the boys quartet: Kousuke is phlegmatic, Takumi is melancholic, Mouri is sanguine and Taira is choleric.
Lady Jewelpet has two sets: the Petit Ladies are Momona (sanguine), Lillian (melancholic), Mizuki (choleric) and Charon (phlegmatic); the Sixth Ranger Elena is leukine. The Princes are Cayenne (leukine), Romeo (phlegmatic), Soarer (melancholic) and Levin (sanguine); the Sixth Ranger Miura is choleric.
Gamagoori is Melancholic, the firm disciplinary officer and enforcer of Satsuki's rules, and the most overt in their loyalty towards her.
Sanageyama is Sanguine, the fight-happy swordsman interested only in finding opponents that can challenge him.
Inumuta is Phlegmatic, preferring to gather and study as much data as possible rather than take an active field role like the others.
Jakuzure is Choleric, Has a driest sense of humor. She also takes great pride in the fact that she's known Satsuki the longest.
The Kiryuin Conglomerate:
Ragyo is Melancholic. She's the Big Bad of the series and an all-around pompous character whenever she gets her moments. She showcases the negatives more than the positive features, had abnormally high wants when she was testing her own daughters, but when they do fulfill them, she is filled with glee.
Nui is Choleric. Arguably Co-Dragons with Rei to Ragyo, and the one who gets the most work done initially while Ragyo sits around and controls REVOCS. Showcases the downsides by being a outwardly nice, extremely crazy inside, and even more insane after normal Ryuko cuts off her arms.
Rei is Phlegmatic. The person who's able to serve at a moment's notice, and without question. However, she showcases the downsides by being an outwardly cold person. Exemplifies this part of her personality in the OVA by being a Defrosting Ice Queen when she heard of Ragyo's death, and was able to be swayed by Satsuki.
Mind Controlled Ryuko is Sanguine. Instead of unable to express her emotions, she says them bluntly, taking them (sometimes literally) to the person she speaks to. Ryuko even kisses Nui, showcasing their love for each other. She balances out the other's negative emotions by being compassionate with them and follows after Ragyo by being WAY over-the-top in battles, but she showcases the negatives by being mind-controlled into finally finding her wants of being with an actual family and comes off as harsh to others.
Sanguine: Yuan is extroverted, fond of wisecracks, and very protective of the people he cares about. He uses fire techniques and wears red.
Choleric: Tokito is quick to anger, perfectionistic, and impulsive. Wears shades of yellow, and fights with a very aggressive energy.
Melancholic: Hishigi is exceedingly introverted and the epitome of The Stoic, analytical and extremely task-focused. He shows textbook signs of severe depression. His color scheme is almost entirely black.
Phlegmatic: Fubuki leads the Taishiro. He is level-headed, cultured and elegant. His fighting style revolves around water and his color scheme is mainly blue.
Superbook (second season): Christopher Peepers (choleric), Joy (melancholic), Uriah (sanguine), and Gizmo (phlegmatic).
Five-temperament ensemble in Tokyo Mew Mew: At first not happy about leading, but accepting her responsibility later on, Ichigo (choleric); Minto (melancholic), Retasu (phlegmatic), the Genki Girl Bu-ling (sanguine), and Zakuro (eclectic).
Trigun more often averts this trope in favor of the Lone Hero or the Power Trio. Adding Wolfwood to the cast has a subtractive effect instead, generating a duo dynamic that leaves Meryl and Millie on the sidelines. When they participate equally they fit the four temperaments: Meryl (choleric), Vash (melancholic, though pretending to be sanguine), Wolfwood (phlegmatic), and Millie (sanguine).
In the original Weiß Kreuz Aya (choleric), Omi (melancholic), Youji (phlegmatic), and Ken (sanguine).
In Wolf's Rain: Tsume (choleric), Kiba (melancholic), Toboe (phlegmatic), and Hige (sanguine).
Your Lie in April: Arima Kousei (melancholic), Miyazono Kaori (choleric), Watari Ryota (sanguine), Sawabe Tsubaki (phlegmatic).
Yu-Gi-Oh! 5D's: Jack (choleric), Yusei (melancholic), Akiza (phlegmatic), and Crow (sanguine).