"...fanboys make passes at girls who wear glasses."
— Neil Sinclair, alt.fan.bgcrisis
Literally, "glasses girl"—one of the classic "sweet girl" stereotypes in anime. Cute enough without going overboard or posing the threat that a more conventionally beautiful girl might, the meganekko is usually pleasant, smart, and clever. And something about the fact that she needs glasses seems to imply an endearing vulnerability that makes her far more accessible to the average guy. After the Yamato Nadeshiko, she's the second-best girl that a boy can bring home to meet his parents and probably the more realistic of the two. Naturally, as with any stereotype, the meganekko can be inverted or subverted, but the vast majority in anime are sweet, smart and — when found in a major role — usually more than a challenge for a male lead to keep up with. Occasionally she's an unpopular character within the story, but not with the fans.
The glasses are the important part, though. Whether she's Blind Without 'Em or not, they are key to her appeal. This is one girl who doesn't need a "Beautiful All Along" transformation; in fact, taking her glasses away is an almost surefire way to reduce her attractiveness!
Sometimes the presence or absence of glasses shows a change in personality, while other times they're a way to give a character a more realistic costume prop.
In Western countries this type of character is rarely popular; when she appears, she is often commanded that The Glasses Gotta Go!, although the Shrinking Violet or cute nerd sometimes invoke this trope.
Glasses on a Hot Librarian or Sexy Secretary are a completely different thing. The Seemingly Wholesome '50s Girl may use glasses to pretend to be this trope.
Boys with glasses are called megane, and can occasionally fit into this type (especially in the Yaoi Genre). (Note that boys cannot properly be called "meganekko"; the term refers specifically to girls.) Other possible male types include Stoic Spectacles, Bespectacled Bastard Boyfriend and Four Eyes, Zero Soul.
When glasses result in sex appeal rather than Moe, they've got Sexy Spectacles.
Not to be confused with Mega Neko. You know, "really big cats". Or MegaTokyo.
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A recent State Farm ad shows guys wishing up things they want. One wish is for a girl that lives in their apartment building. Instead of being a token hot blond, she's actually more cute than sexy, and wears (*gasp*) glasses.
The Unfortunate Implications of basically abducting a woman into an apartment with three bros led to the ad being widely mocked.
The OS-tan version of Windows 2000 has this look, which overlaps with both Hot Librarian and Sexy Secretary, given she's a personification of an operating system.
The Rhapsody banner ads on various sites(ie. this one) feature a highly adorkable woman, apparently named Molly.
Ah! My Goddess: Also included as a visual gag in the TV series. Originally Skuld is able to see gremlin-like "bugs" via specialized goggles. In the show, she and the others wind up wearing 'goggles' that are obviously just a stylish set of tinted glasses.
Later a genuine Meganekko, Hasegawa Sora, showed up in the cast.
Ai Kora: Yukari, who even has a fangirl of her own with a glasses fetish. The mangaka even devoted a whole chapter to this topic: when Yukari feels insecure about her looks, thinking that her glasses make her ugly, the aforementioned fangirl takes her to a cafe full of glasses fetishists, who make her model different outfits and glasses and repeat that the glasses enhance the look. They even teach her 'glasses techniques', like peering over the glasses, etc. Of course, when she meets the protagonist and tries it out, he doesn't notice, because his fetish ain't her glasses.
Axis Powers Hetalia: There are several male Meganekkos but the ones who resemble the archetype more closely are Estonia, Austria, Canada and Thailand. America does not fit the stereotype, but his glasses represent Texas; Sweden, meanwhile, looks like the Stoic Spectacles type, but he almost fits because of his Huge Schoolgirl-like personality.
Finally, a female Meganekko is introduced in the person of Monaco.
England's and Sweden's Nyotalia versions also have glasses. Strangely, America's and Austria's don't. Canada's kept them.
Among the Japanese prefectures, we have two meganes and one meganekko. The boys are Touyama◊ and Ibaraki◊, the girl is Fukui.◊
Azumanga Daioh: Yomi is both an honors student and an Academic Athlete, as her grades are consistently among the highest at the school (usually placing second below Chiyo). She's also the most sensible and levelheaded student in Yukari's class, which is why she's often the series' straight man.
Berserk: Cute Witch Schierke could count, since she wears a pair of rounded glasses when she draws seals on Guts's and Casca's brands.
Black Lagoon: Possible subversion: Greenback Jane wears glasses and is very smart in certain areas. She's also yappy, bitchy and full of herself. She is also the only character to have panty shots. Roberta on the other hand plays with the trope in that she is at her core an Ax-Crazyimplacable womanDeterminator, but also tries to be Garcia's loving and gentle "Roberta," which she can only be with her glasses.
Candy Boy: Kanade, the "younger" twin sister, wears glasses. However, in most of her appearances, she wears contact lenses instead.
Chocotto Sister: Chitose, the landlady, wears thick glasses and is very self-conscious about it. A boy calling her a "four-eyed dog" in high school is a major emotional hurt of hers and Haruma's complimenting her on how she looks wearing them is a large part of why she falls for him.
CLANNAD was mostly lacking in Meganekkos, but Sanae delivered in spades with her disguise in After Story. Tomoyo at one point starts wearing glasses as well.
Code Geass: Nina Einstein was like this for much of the first season. She appears this way for a good portion of season one because she barely says anything. But then she gets weirder, and weirder, and weirder. And then worse. And in the end, she getsbetter. Wow.
She also has elements of Beautiful All Along in episode 9 of R2 when she attends the wedding banquet for Tianzi and Prince Odysseus as Schniezel's date, in a pretty gown and without her glasses. Although whether this actually makes her more attractive or not is up to personal taste.
11eyes: Yukiko Hirohara. Turns out she wears the glasses to keep her cold-blooded other side in check.
Fairy Tail: Levy wears glasses whenever she concentrates on her work, she and Laki represent this for the younger guild members.
Then we have Evergreen, who is somewhat more refined and eccentric then the usual example but qualifies nonetheless.
Fruits Basket lampshades this when Tohru and Yuki go to Ayame's shop and meet his assistant — a glasses-wearing girl in a maid outfit who proceeds to explain the long tradition of Meganekko to HeroicBSODing Yuki.
FLCL: Parodied with Ninamori Eiri. She really does need glasses, which she hides by wearing contacts. Even when her part in a school play calls for her to wear glasses, she uses lensless ones over her contacts to preserve her secret.
At one point in the story, Riza pops out the lenses from Fury's glasses and parades around in them, as a (somewhat ineffective) disguise. She also wears glasses in several omakes and a video game. The "reason" for all this seems to be she just looks hot in them.
Discussed Trope in the movie's extras when the director Mizushima and Rie Kugimiya (Alphonse) talk about glasses for three minutes and invoke the popularity of the meganekko.
Rikka of DokiDoki! Precure, though, like Tsubomi, she wears her occasionally. Unlike Tsubomi, who's given contacts, it appears Rikka's just a little near-sighted, as she'll wear them if she needs to see something too far away.
From G Gundam we have Natasha Zabikov (Argo's warden) and Bunny Higgins (one of Chibodee's Gals).
Thing is, Natasha isn't exactly what you'd call sweet.
Haibane Renmei: Hikari. In contrast to Kana and Kuu, both of whom dress rather Bifauxnen, Hikari with her glasses looks the most like a smart young lady.
Haiyore! Nyarko-san: Nyarko tries to invoke this in episode 9, but it has no effect on her love interest Mahiro. She insists it's because he doesn't understand the power of the glasses, and makes him try on a pair...which makes Nyarko herself melt into a puddle of Squee. Then Cuuko tries the same trick on Nyarko, who coldly responds "Quit playing with store merchandise", causing Cuuko to sob about the Double Standard.
Hanaukyō Maid Tai. Head maid of the Technology department Ikuyo Suzuki, at least some of the time.
Haruhi Suzumiya: Subverted (and lampshaded) with Yuki Nagato, when she loses her glasses, Kyon comments that he "doesn't really have a glasses fetish" anyway and refuses to explain what the term means when she asks.
Looking at the character design for the anonymous female classmates suggests that the character designer might have had a glasses fetish. Since we're talking about Noizi Ito here, that may not be too far off the mark (and glasses are one of her more modest fetishes).
Haruhi seems to be quite familiar with this trope. One of her more plot-related molestations of Mikuru featured her borrowing Yuki's glasses, perching them on Mikuru's nose, and proclaiming that poor Mikuru is now the perfect moe blob.
Hellsing of all places plays with this. The Iscariot nun Yumiko is a simple, sweet, shy girl with her glasses on. Take them off and she becomesan impossibly psychotic, enragedkilling machine. There are three other Girls With Glasses of note in the series, but while they may be cute none of them (including Integra Hellsing herself) have even a nodding acquaintance with "sweet".
Integra embodies this trope when she was a teenager.
Rip Van Winkle was this as a human. She gives an air of it, before being violent, now.
Hidamari Sketch: Sae, who's generally pleasant, consistently described as "beautiful" in the manga's character profiles, and is called "a walking encyclopedia".
High School Girls: Subverted, where the sweet-looking glasses girl of the group is the dimmest and most perverted out of all of them.
Hunter × Hunter: Shizuku has the look down. The "sweet" aspect is diminished somewhat by the fact that she's a remorseless murderer.
Ichigo Mashimaro: Matsuri is kind and studious, if easily fooled. She's a cute kid with or without glasses, but as she's Blind Without 'Em, and kind of clumsy at the best of times...
Kiddy Grade: Mercredi and Vendredi. Strangely, though, Mercredi demonstrates that she actually doesn't need them at all, when she puts them down as Alv and Dvergr invade HQ. Later, she shows up again, but in her real form, Pfeilspitze, with a different costume and no wig nor glasses, showing that she either wears contacts or doesn't need them at all.
Mako Mankanshoku briefly sports a pair as the Fight Club President, though they're more Stoic Spectacles to contrast her usual bubbliness.
Kimagure Orange Road: Manami Kasuga is one of the earlier embodiments of this trope. A cute, sweet, responsible girl, she keeps the Kasuga household together. Be warned - she can't see anything if she loses her glasses or contacts.
K-On!: Nodoka is pretty calm and friendly, and like everyone else, mostly gets annoyed with Ritsu. Mio shows she can pull off the look by borrowing(?) her glasses temporarily, leaving her with 3s for eyes.
Kurosagi Corpse Delivery Service gives us designer frame-wearing Ao Sasaki, who is even called a Mega Nekko in the notes at the back of the English-language volumes of the manga. Even when she's talking to another character in a spiritual form, she still has her glasses on
Kyo Kara Maoh!: Ken Murata shows up as the male variant. Though he eventually turns out to be The Chessmaster, he never quite entirely loses the Meganekko side, either.
The Law of Ueki: Rinko Jerrad. Mori Ai also has glasses—and her powers are based around them—but she wears them atop her head rather than over her eyes.
Love Hina: Naru Narusegawa after the first few episodes when her Nerd Glasses transform into "pretty girl" frames. Thing is, she was doing it for the same reason as Miu Fuurinji above: to avoid attracting the attention of boys.
Loveless has so many meganekko and megane it's believed that the author has a fetish for it. Some female examples are Shinonome-sensei, Kouya, and Nana.
Lucky Star: Miyuki. Lampshaded when Konata comments that her glasses are part of her Moe appeal. She also blurts that her email address is (translated) IXTRALUVGIRLSWITHGLASSES, while in a crowded bus, earning some odd looks.
Mahou Sensei Negima!: Haruna Saotome, Satomi Hakase, Chisame Hasegawa when she's not being net idol Chiu. Kazumi Asakura and Evangeline A.K. McDowell sometimes wears glasses. The same goes for Nodoka's cosplay form in the Gag SeriesNegima!? and the mostly manga-only character Tsukuyomi.
Negima tends to subvert the "sweet girl" part. Haruna is downright scary, Chisame is far more snarky than sweet, Hakase is a Mad Scientist, and as for Tsukuyomi...HOLY SHIT.
Mai-HiME and Mai-Otome: Yukino Kikukawa, whose shyness provides a perfect balance for her very outspoken best friend Haruka. Her Mai-OtomeExpy, Irina, also wears cute-girl frames, but they're smaller and rounder.
On the other hand, Chie from the same series seems like she'd be a meganekko, but turns out to really be a Bifauxnen.
Martian Successor Nadesico: Hikaru. One character specifically notes that she's cute with her glasses off, as if she weren't cute enough already. It's debatable.
One is also suspicious, given how Genre Savvy she (and most of the rest of the crew) is, that she might just be wearing them for effect, especially since all the other glasses on the show are of the Scary Shiny variety, while hers aren't.
Medaka Box- Kikaijima Mogana is one of these when she gets out of her swimsuit. Onigase Harigane is one too.
Midori no Hibi: Seiji's cantankerous delinquent big sister is also a bit of a subversion, although her glasses are better explained by her Office Lady outfit. Played straight by Seiji's first crush Yukino, even if she did grow up to be a Yaoi Fangirl.
Nagasarete Airantou: Subverted where Chikage initially seems to have the proper personality but ends up having Scary Shiny Glasses and is as crazy as the other girls on the island. However, played straight with her mother Shizuka, wherein she's pleasant, and clever to fool even the lead male.
Pani Poni Dash!: Two of the main six girls Rei and Miyako. Ichijou has a theory that their glasses are the reason they do well on tests. (Suzune tries this out as a joke in the manga, but Ichijou is dead serious.) Behoimi later becomes a meganekko when she quits being a Magical Girl.
Trying to put the word "sweet" anywhere near Rei is liable to induce explosive reactions.
Ranma 1/2: The first two times Ranma Saotome disguised himself as a different girl, rather then just using his female form and trusting to the sucker's lack of knowledge/wits to disguise him, he pretended to be one of these as part of the disguise. One might almost think Ryoga was attracted to Meganekkos, given how easily he fell for these disguises, but considering the ease with which he also fell for Ranma's rudimentary disguise (Ranma in girl form wearing a volleyball uniform), and when Ranma claimed to be his maid, well...
Somewhat unique among "cute girl" stereotype-types, as during ROD the TV it's mentioned that she's over thirty. Oddly, her looks haven't changed at all since her first manga appearance, when she stated (honestly) that she was 23.
Those aren't her glasses anyway.
If you search Google Images for Meganekko, it suggests Yomiko Readman as a related search.
Nenene Sumiregawa in the same series is also quite the Meganekko herself, though her personality doesn't fit the usual stereotype.
Real Drive has Secretary-General Erika Takanami and the android modeled after her, Holon.
Revolutionary Girl Utena: Anthy's gentle and objectively intelligent nature seems to fit the role, though her apparent ditzyness is explained in a dark and extreme subversion later on.
Also subtly lampshaded in that the finale reveals that Anthy doesn't even NEED to wear glasses, and was seemingly wearing them at Akio's order, most likely to make her look more submissive and timid. When she finally breaks ties with him, she illustrates this by taking off her glasses and leaving them on his desk, and heading out into the world without them.
This is hinted at in one of the next episode previews much earlier in the series where Anthy casually remarks that she can see better WITHOUT her glasses.
Not really. It refers to the Freaky Friday episode where Anthy and Utena switch bodies, and as such Anthy is using Utena's eyes during the preview.
Rizelmine: Papa C's daughter has a thing for the main character, and is usually wearing glasses. When in glasses, she's shy and unsure. Take off her glasses and she becomes a horny little predator. Considering the nature of the anime, it is likely Genre Savvy.
Sailor Moon: Mizuno Ami (Amy in The Nineties English dub) is an interesting example, though they were an obvious metaphor for Ami's introversion and fear of making friends. In the original manga, she just used them for reading occasionally, and the anime rarely showed them at all. However, in Pretty Guardian Sailor Moon, she seemed to have caved to fan appeal, and wore them more or less all the time in the earlier episodes.
Sachiare!: Tomoharu's bride Sachiko is ungodly cute already, with her glasses on it gets taken to lethal levels.
Saki: All four teams from the Nagano prefectural tournament have one. Kiyosumi has Mako, Ryuumonbuchi has Tomoki, Kazekoshi has Miharu, and Tsuruga has Kaori. They all somehow ended up facing one another in the second round.
The list goes on in the Nationals, to the point at which Usuzan and Miyamori seem the only exceptions. Shiraitodai has Takami, Rinkai has Satoha, Eisui has Tomoe, Himematsu has Kinue, Senriyama has Hiroko and their coach Masae (Kinue's cousin and mother, respectively), Shindouji has Yoshiko, Kentani has Kozue, and Koshigaya has Sophia. In a less obvious case, Yuu of the Achiga team wears glasses whenever she has to look closely at something.
Shoujo Sect: Honda Shinobu has bad eyesight. Within the story itself she wears contacts, but a short series of throwaway gags features her wearing her specs as she, Kirin, and Maya discuss how a girl in glasses should go about kissing another girl in glasses (the series is essentially a lesbian hentai). Maya worries that if one of the girls removes her glasses it will eliminate her Moe Moe factor, but Kirin assures her that a true Meganekko is always a Meganekko in essence, even if she isn't wearing glasses.
Yoko does this too, during her time as Hot Teacher Miss Yomako. She seems to want to invoke the trope as a teacher as she tries to project a Mama Bear air concerning her students.
Tiger & Bunny's Tomoe Amamiya (aka, the woman who's implied to have become Kotetsu's wife) was one of these, at least in high school. Kotetsu used to refer to her as Ms. Glasses before they switched to First Name Basis.
Thriller Restaurant has Anko, but she, while usually is shy and soft-spoken, can also lash out when too angry.
In Spider-Men the Peter Parker of the 616 Universe, who started off wearing glasses himself states that his world's MJ wears contacts, but this seems highly unlikely given that she's never actually been seen anywhere near glasses.
Mockingbird used to have contacts but now wears spy glasses that also doubles as corrective lenses for her farsightedness.
X-Men: Before she was outed as a mutant, when she was still Deputy Director of DARPA, Raven "Mystique" Darkholme was never seen without her rather severe hornrims; since, like her clothes, they were literally part of her.
Thessaly the murderous witch has worn glasses ever since her first appearance in The Sandman. Presumably, she wears them to convince others that she's harmless.
Skin: For some reason, during the time before 'going blind' became too serious, Shizuka Kawai was one for a short period of time. After her blindness took a nasty turn and surgery was required, this quickly stopped.
Subverted with Myrtle Edmonds from Lilo & Stitch; she wears glasses and looks as though she'd have a shy, nerdy personality, but she's really the Alpha Bitch. Played much straighter with her mom in the TV series, though.
The Day After: Marilyn Oakes, played by Kyle Aletter, is the doctor's daughter who foolishly exposes her glasses to temperatures that are higher than recommended.
Day for Night: Francois Truffaut's 1973 movie. Joelle, the script girl, played by Nathalie Baye. Catch Me If You Can director Steven Spielberg said when they needed to find an actress to play Leonardo DiCaprio's French mother he remembered liking the script girl from Truffaut's movie and cast Baye.
The Faculty: Famke Janssen's Ms. Burke up and down, from the glasses to the crippling shyness. When circumstances lead to the glasses coming off, this all changes.
Jurassic Park: Dr. Ellie Sattler (Laura Dern), already hot, gets just a little bit hotter in the movie's scene in the park's tourist museum and dinosaur egg laboratory when she puts on a pair of glasses.
In the Inspector Morse book "The Secret of Annexe 3", Sarah Jonstone, the receptionist of the Haworth Hotel, has "almost comically large" spectacles, and ticks the boxes for being pleasant, smart and clever. By the end of the book, she and Morse are dating.
Degrassi The Next Generation: For the students, there's Liberty, and then later, Clare, before she got her laser eye surgery, another bane of glasses fetishists, and Maya after her. For the teachers there's Mrs. Oh.
Doctor Who: The TenthDoctor himself frequently dons his so-called "Brainy Specs" quite frequently. As he exposits when he encounters his fifthincarnation, he doesn't really need them, he just thinks they make him look clever. A side effect of this is that they make him adorable, but that might just have something to do with the fact that he's played by David Tennant.
Dollhouse: Bennett which is actively lampshaded by Topher, who out and out says she's almost perfect. Except for the whole "violently seeking revenge on Caroline's body" thing...
Topher: Glasses on a chain!
Victor!Topher: For the Win!
Eleventh Hour: Agent Young has a pair of glasses that appear whenever she and Hood are looking at computer screens.
Gilmore Girls': Lane Kim. Lampshaded when she used contact lenses in an episode and both her mother and her boyfriend criticized her decision.
The episode, "You're So Vain, You Probably Think This Zit Is About You'' revealed that Lily wears contacts, and when her dog eats them, she has to wear glasses. Even though she hates them, they put her squarely into this trope.
Miley wore a pair while she was Lily's "lawyer" in the episode "You Are So Sue-able To Me".
The Middleman: Referenced in the show's podcast, when the creator is asked why Wendy Watson doesn't wear her glasses more. The response given is that she generally wears her glasses in civilian mode, and removes them in crime-fighter mode as a Clark Kent/Superman reference.
NCIS: Director Shepard would occasionally put on glasses.
The episode "Singled Out" featured a Navy lieutenant as a kidnap victim who fit the trope, and in the same episode, Ziva falls into the trope when she goes undercover as a geek resembling the Navy lieutenant.
Male example; Jimmy Palmer. Agent Lee definitely thought so.
In The Office (US), this trope was brought up in the episode "Did I Stutter?" While already the show's eye candy, Pam gets even hotter when it's revealed she wears contacts and has to wear her glasses when she forgets her solution. While Michael doesn't like them, she finds herself the object of Kevin's advances after he reveals he has a glasses fetish.
Don't forget Billy and Kimberly switched in the Freaky Friday episode. That episode served as fetish fuel for many a preteen and young teen troper (such as myself).
Power Rangers Lightspeed Rescue: Ms. Fairweather. Always wearing glasses, very hot AND presented as very hot - rare for Western television. The Green Ranger was particulary smitten with her, and they eventually hooked up and got married. (one of the very few actual romances in any series of Power Rangers).
Power Rangers Lost Galaxy: Kendrix is considered a Meganekko by some...emphasis on the some, as most fans consider her to be pretty homely with or without glasses.
More like Hollywood Homely, since responses I've seen range from "she's not as hot as the other pink rangers" to "she's the hottest girl I've ever seen" to "I had the biggest crush on Kendrix as a child/teen".
Stargate SG-1: The already ridiculously hot Colonel Samantha Carter of appeared in the alternate time-line episode "Moebius" where instead of being a Colonel Badass and an Action Girl, she was a Hot Librarian type, complete with the coke-bottle obviously fake-lensed glasses.
The X-Files: Scully, whose glasses are only worn when she composes her reports to her boss on a computer, are put in for the sole purpose of this effect. Especially because they appear to have no optical qualities at all, and are just clear glass.
Arrow: Felicity Smoak. In one episode she went to a party without her glasses and just looked gorgeous instead of "Smoaking" Hot!. Sorry.
Brazilian actress Tania Khalill at the novela "Fina Estampa".
Guitarist/vocalist Sheena Ozzella of the indie rock band Lemuria definitely qualifies when she's wearing glasses. Just look at her!
Taylor Swift. In the video for the song "You Belong With Me", she plays dual roles as a cheerleader and as a nerdy neighbor girl. For the nerdy neighbor girl part she dons a pair of glasses, which not only fail to make her look unattractive but actually make her even more beautiful.
ZZ Top. The video for "Legs". The featured girl in the video, in addition to having the great set of legs the title describes, she also wears a sexy set of glasses for the first 45 seconds of the video.
Adventures in Odyssey: Katrina Shanks. Medium notwithstanding, she fits the mold of a meganekko, she acts like a meganekko, and just to dispel any remaining doubt, the official artwork (only seen in the older, less stylized pictures for good measure) depicts her as a reserved, bespectacled meganekko.
Lucy Cunningham-Schultz, the resident Intrepid Reporter for the Odyssey Owl, is another example.
The Ace Attorney series has Maggey Byrde; Adrian Andrews, an actor's manager; Penny, a film set designer; and to a goggle extent, Ema Skye.
Harvest Moon: Many of the games in the series have a character like this whom you can score as a potential wife: Maria in the Flower Bud Village saga, Mary in the Mineral Town saga and Flora in Forget-Me-Not Valley.
Ritsuko isn't a typical cutesy meganekko though - she just projects that persona. In fact, she's snarky and more than a little sneaky, having been known to astroturf an Internet message board to drum up support for herself.
Metal Gear Solid 2: Emma Emmerich admits that she simply wears glasses for show, and because her step-brother Hal wears glasses. And Hal, thanks to those Moe "Sweet Snake" figurines that have recently popped up, can qualify for being a male example of this trope.
In some Easter Eggs, you find out that both Raiden and Snake seem interested in her.
Snake: Do I need to clear things with Otacon before I ask her out?
Otacon (Hal) definitely qualifies. Certain segments of the fanbase had cause to mourn when he took them off for a bit of MGS4 because of character development. He returns to wearing them by the end of the game.
Fio Germi from the Metal Slug series is always seen wearing glasses.
Persona 3 has Chihiro Fushimi, the adorably shy and meek class treasurer, as one of the Main Character's social links, and a datable one at that (woo!). She makes a cameo in Persona 4 - two years older, more confident, and somehow even cuter, leading every one of your characters to feel swoony or envious at her all-encompassing beauty.
Persona 4 practically enforces this trope on the entire party.
Resident Evil 4: Ingrid Hunnigan, although Leon clearly doesn't believe in this trope, as he doesn't ask her out until she loses the glasses.
Rule of Rose: Meg, the Wise-Looking Princess, acts as a secretary for the Aristocrat Club and the spokesperson of the Rose Princess, and has apparently invented at least some of the torture devices used by the Club for keeping discipline. Other than that, she is mainly characterized by her obsessive devotion to Diana, who really doesn't appreciate the attention.
Star Ocean: Cute Clumsy Girl Sarah Jerand seems to be an archetype laundry list with some wings attached. Most players, however, hate her because her personality traits are exaggerated to the point of ridiculousness in most of her featured cutsenes.
Team Fortress 2: Miss Pauling, the Administrator's assistant, introduced in one of the Administrator comics. She doesn't hesitate to manipulate Soldier and Demoman into killing each other, mind, but she has the look. (It helps that she's still less cold than her boss. This says a lot about her boss.) As the comics and videos progressed, she becomes one of the most important female characters, remaining cute despite her proficiency with deception and murder.
In the video "Expiration Date", Scout explicitly mentions Pauling's "slightly crooked" glasses as a fetish of his.
Time Hollow: Emily. To be mean, you can take her glasses. She gets in a horrible accident off-screen.
4 has Tsugumi Godo (Bookworm who, in a subversion, is a Tsundere extraordinaire)
Girl's Side 1 has Shiho Arisawa (Kind and studious Huge Schoolgirl), and Sakuya Morimura (a sweet young man who loves flowers) as the male variant.
Girl's Side 2 has Chiyomi Onoda.
Touhou: Surprisingly for an Improbably Female Cast, there's only 4 characters that fits this trope: Rikako Asakura, Luna Child (when she's reading), Mamizou Futatsuiwa, and Kosuzu Motoori (again, for reading).
There's a FSN doujin ("Beat Your Fate") where we see Sakura give Rider the glasses, secretly thinking this will make sure Shirou doesn't fall for her — at which point he turns out to be a "glasses man". The rest of the harem immediately start trying pairs on. Sakura has no choice but to do the same... and Shirou tells her she looks more plain with glasses.
Sono Hanabira Ni Kuchizuke Wo: Played with by Kaede, who is shy and kind and is the Class Representative but is really only an average student. Lampshaded by Sara, who asks if Kaede is an honour student, and admits that she only thought she was because her glasses and plait gave off that impression. However, while Sara doesn't have any particular feelings about Kaede's glasses, she dislikes the personality they give her, preferring a more masculine, Bifauxnen type of personality, which Kaede only reaches when she removes her glassesand undoes her hair.
Tsukihime: Ciel, though she probably has better eyesight than most.
The Dreamland Chronicles: Nicole is one of these. Glasses are a little smaller than the usual fare, but she certainly fits the trope.
Eerie Cuties brings us Chloe, the Meganekko Moesuccubus — who eventually gets her own spin-off, Dangerously Chloe, but loses the glasses in the process (because her supernatural beauty would melt them).
Frivolesque: EVERY female character in Frivolesque save for action girl Gaia and the voluptuous Liliane (who has sunglasses on instead) is a meganekko in some way.
Fetch Quest: Saga of the Twelve Artifacts gives us Felicia, who double-subverts the "sweet, modest girl" part of this: She manages to hold up on her own against thieves, but when confronted with Lionel "the Lucky", she almost immediately goes back to being this.
Girl Genius: Agatha, the title character. Her cousin Theo fits the rarer, gender-flipped megane category.
Homestuck: Jade Harley is a classic example. In what could probably be attributed to Author Appeal, half the main cast wears glasses, so she's not the only one - other examples present include Vriska Serket and Terezi Pyrope and Feferi Peixes. Jade's brother John Egbert is a male example. Also present is Jane Crocker, John and Jade's genetic mother.
It's Walky!!: Parodied when Billie gets glasses, not only does Danny find her even more attractive than before, but several guys who didn't care about her before (Joe and Howard in particular) begin hounding her until she threatens to get contacts.
RPG World: Detestai of the defunct webcomic is a catgirl who was given magic glasses by her ex-boyfriend, the villain, that turn her from a gentle kitten to "evil to the glasses." Also, she's rather tall. Does that make her a Mega Neko?
Sticky Dilly Buns has Ruby, who is cute in her way and book-smart, and definitely has the big glasses. Unfortunately, she also has an unstable temper and doubts about men.
The Wotch: As of a long-term Gender Bender with accompanying memory alterations for everyone else, Ivan "Yvonne" Bezdomny has apparently been thrown headfirst into Meganekkodom. Compounded with all of the transformation's other consequences...
"Homeless Blog": The loneliness is only magnified by the attention I do receive. Somehow I have a "quiet, nerdy thing goin' on" that some guys find attractive. This scares me.
Gaia Online, with its strong anime influence, has several.
Daria: The title character, though she may be something of an inversion — it's pretty well established that Daria is naturally attractive and dresses down to avoid attention, so it's not too clear if the glasses work for her or against her.
Hey Arnold!: Phoebe, the show's official Meganekko, and in one episode Rhonda is forced to get glasses due to her waning vision. At first, she absolutely hates them because it drops her to geek status. However, she later comes to accept them, and gets a new, sleeker pair of glasses at the end of the episode.
The Legend of Korra: Zhu Li, the Cloud Cuckoo Landers Minder to Varrick, certainly fits in both appearance and personality, patiently tolerating her employer's eccentricities and executing his various wild schemes. Except she's actually Varrick's Dragon, and described by him as a "cold, heartless war machine."