* The four protagonists of ''Anime/TheAnthemOfTheHeart'', which is even lampshaded within the film. Natsuki is sanguine. She's the friendly, hardworking cheer captain of the team. She's accommodating, but can give in to peer pressure. Daiki is choleric. He's a passionate and hardworking leader but can be demanding and rude. Takumi is melancholic. He's introverted, avoids talking about his feelings, and is seemingly apathetic, but is considerate, decisive, and sensitive. Jun is phlegmatic. She's self-deprecating and passive to the point of having debilitating anxiety, but is compassionate and kind.
* ''LightNovel/BakaAndTestSummonTheBeasts'':
** Boys: Akihisa Yoshii (sanguine; a friendly ditz), Yuuji Sakamoto (choleric; the leader of the boys and a plan-maker who's determined to climb the school ladder), Hideyoshi Kinoshita (phlegmatic; usually keeps his cool and is the most sensible of his classmates), and Kouta Tsuchiya (melancholic; taciturn and untalkative, only cares about taking perverted pictures).
** Girls: Minami Shimada (choleric; she's an easily provoked {{Tsundere}}), Mizuki Himeji (phlegmatic; gentle and friendly), Shouko Kirishima (melancholic; a quiet {{Yandere}} completely focused on the object of her affections), and Aiko Kudou (sanguine; friendly, easy-going and mischievous).
* Although everyone in ''Manga/BigWindup'' gets his/her turn to shine, the story tends to focus on four core members of the baseball team:
** TheCaptain Hanai (choleric), TheStrategist Abe (melancholic), FragileFlower Mihashi (phlegmatic), and CloudCuckoolander Tajima (sanguine).
* ''Manga/BlackLagoon'':
** SociopathicHero Revy (very choleric), WideEyedIdealist Rock (melancholic), BaldBlackLeaderGuy Dutch (phlegmatic), and MissionControl Benny (sanguine).
** The four most feared women within the series:
*** Revy (Choleric): Easily the most vile and foul-mouthed of the four, disregarding anybody (and anything) that dares to piss her off.
*** Balalaika (Melancholic): Analytical and resourceful when getting information, rather its through her men or destructive means.
*** Roberta (Phlegmatic): A seemingly humble and gentle maid, who disguises her unstoppable killer intent with conviction and modesty.
*** Shenhua (Sanguine): Chatty and openly sociable to everyone, deadly in a glamourous way with a hint of [[DragonLady Oriental charm.]]
* The original ''Anime/BubblegumCrisis'':
** Choleric: HotBlooded badass Priss.
** Melancholic: [[TheStoic Stoic]] leader Sylia.
** Phlegmatic: Linna the average Jane.
** Sanguine: Perky and [[TheDitz ditzy]] Nene.
* ''Manga/ChronoCrusade'':
** Sanguine: [[NunTooHoly Rosette]] (HotBlooded, cares a lot about others but has a violent temper).
** Choleric: [[MsFanservice Satella]] (prideful, determined, and focused on her revenge).
** Melancholic: [[HeroicAlbino Azmaria]] (a prodigy singer, very caring about others, but insecure and hard on herself).
** Phlegmatic: [[FailureKnight Chrono]] (compassionate and nurturing, but easily discouraged)
* ''Anime/DeathNote'':
** The main cast:
*** [[AxCrazy Light]], who is concerned with saving the world from criminals by killing them and develops a [[AGodAmI god complex]] (choleric)
*** [[TheStoic L]], who always tries to solve cases and rarely sleeps (melancholic)
*** [[{{Cloudcuckoolander}} Misa]], an energetic girl who is obsessed with Light (sanguine)
*** Ryuk, who doesn't care for anyone and has no real goal (eclectic)
*** [[TokenGoodTeammate Rem]], who is like a mother for Misa and would do anything for her (phlegmatic).
** Also, the police officers:
*** [[{{Workaholic}} Soichiro]], who always works and is the most concerned with the case (melancholic)
*** [[TheDitz Matsuda]], the youngest and most childish member (sanguine)
*** [[HairTriggerTemper Aizawa]], who can get angry pretty easily (choleric)
*** [[TheQuietOne Mogi]], who talks the least and is the most calm (phlegmatic).
* The four Dark Masters in ''Anime/DigimonAdventure'' fit this trope:
** Choleric: The leading element Piedmon.
** Melancholic: The cold and calculating EvilGenius Machinedramon.
** Phlegmatic: Pragmatic Metalseadramon, who's a good boss towards his minions as long as they don't fail him.
** Sanguine: Puppetmon, with his HotBlooded PsychopathicManchild demeanor.
* ''Anime/DigimonSavers'' has two sets:
** The main cast:
*** HotBlooded Marcus (choleric), TeenGenius Thomas (melancholic), FauxActionGirl Yoshi (phlegmatic), and WildChild Keenan (sanguine).
** The original expedition:
*** GeniusBruiser Spencer (choleric), BigBad Kurata (melancholic), CoolOldGuy Yushima (sanguine), and {{Good Parent|s}} Criers (phlegmatic).
* ''Anime/DigimonTamers'': Rika (choleric), Henry (melancholic), Takato (Phlegmatic) and Jeri (sanguine).
* In ''Anime/{{Endride}}'', the central four [[LaResistance Ignauts]] demonstrate these traits:
** Sanguine: Demetrio, the [[MagneticHero dynamic]] and [[IdealHero idealistic]] RebelLeader.
** Choleric: Louise, the [[BrokenBird cynical]] but lively [[GirlyBruiser bruiser]].
** Melancholic: Felix, the [[TheQuietOne taciturn]] but [[UndyingLoyalty ultra-loyal]] follower.
** Phlegmatic: Eljuia, the [[NonActionGuy peaceful]] and empathetic [[FaintingSeer seer]].
* ''Manga/FistOfTheNorthStar'' has the four Hokuto brothers: [[NobleDemon Raoh]] (Sanguine), [[DirtyCoward Jagi]] (Choleric), [[GoodIsNotSoft Kenshiro]] (Melancholic), and [[IncorruptiblePurePureness Toki]] (Phlegmatic).
* ''Manga/{{Hellsing}}'' has three:
** The Hellsing Organization: Alucard (choleric), Seras (phlegmatic), Walter (melancholic), Pip (sanguine) and Integra (leukine).
** The Iscariot Organization: Alexander Anderson (sanguine), Heinkel Wolfe (choleric), Yumie Takagi (phlegmatic), and Enrico Maxwell (melancholic).
** Millennium: The Major (sanguine), The Captain (melancholic), Doctor (phlegmatic) and Schrodinger (choleric).
* ''Franchise/{{Jewelpet}}''
** ''Anime/JewelpetHappiness'' has the boys quartet: Kousuke (phlegmatic; the gentle and low-key guy who's a little spacey but always ready to give advice), Takumi (melancholic; the academically accomplished cold perfectionist), Mouri (sanguine; the most openly friendly and sociable of the boys), and Taira (choleric; the aggressive but insecure guy who always feels he has something to prove).
** ''Anime/LadyJewelpet'' has two sets:
*** The Petit Ladies are Momona (sanguine; open, friendly and cheerful, if an easily distracted klutz), Lillian (melancholic; cold, initially friendless and focused on her goal of becoming the best Lady), Mizuki (choleric; practical, blunt and easily exasperated) and Charon (phlegmatic; a shy bookworm and a NiceGirl); the SixthRanger Elena is leukine (for embodying everything considered essential in a Lady).
*** The Princes are Cayenne (leukine; well-balanced personality among the rest of the boys), Romeo (phlegmatic; a real gentleman, open-minded and considerate), Soarer (melancholic; intelligent and methodical, notably dislikes it when things don't go by the book) and Levin (sanguine; impulsive LargeHam and a NiceGuy); the SixthRanger Miura is choleric (as the cynical and mocking resident bad boy).
* ''Manga/JoJosBizarreAdventure Startdust Crusaders''
** Joseph Joestar (eclectic), Jotaro Kujo (choleric), Noriaki Kakyoin (phlegmatic), Mohammad Avdol (melancholic), and Jean Pierre Polnareff (sanguine).
* ''LightNovel/KaraNoKyoukai''
** Ryougi Shiki (melancholic), Kokutou Mikiya (phlegmatic), Aozaki Touko (choleric), Kokutou Azaka (sanguine)
* ''{{Anime/KillLaKill}}'' has three groups:
** Main Characters:
*** Choleric: [[TheHero Ryuko]] -- {{Hot Blooded}} nature and single minded determination to get the answers she wants out of Satsuki.
*** Melancholic: [[KnightTemplar Satsuki]] -- Focuses on accomplishing her plans and using [[TheUnfettered whatever means necessary]] to do so, and [[NoNonsenseNemesis cares very little for anything that hinders her progress]].
*** Phlegmatic: [[TheStoic Senketsu]] -- Mostly interested in assisting Ryuko's quest and protecting her from harms way, as well as try to keep her extreme temper under control.
*** Sanguine: Mako -- The {{Plucky Comic Relief}} interested in having fun with Ryuko, and seems to know just what to say in a given situation to snap people out of their funk.
** The Elite Four:
*** Sanguine: Sanageyama -- [[BloodKnight Fight-happy swordsman]] interested only in finding opponents that can challenge him.
*** Melancholic: Gamagoori -- Firm disciplinary officer and enforcer of Satsuki's rules, and the [[UndyingLoyalty most overt in loyalty]] towards her.
*** Phlegmatic: Inumuta -- Preferring to gather and study as much data as possible [[NonActionGuy rather than take an active field role]] like the others.
*** Choleric: [[DeadpanSnarker Jakuzure]] -- A driest sense of humor. She also takes great pride in the fact that she's known Satsuki the longest.
** The Kiryuin Conglomerate:
*** Melancholic: [[TheBaroness Ragyo]] -- The {{Big Bad}} of the series and an [[EvilisHammy all-around pompous]] character whenever she gets her moments. She showcases the negatives more than the positive features, had abnormally high wants when she was testing her own daughters, but when they do fulfill them, she is filled with glee.
*** Choleric: Nui -- Arguably {{Co Dragons}} with Rei to Ragyo, and the one who gets the most work done initially while Ragyo sits around and controls REVOCS. Showcases the downsides by being [[CuteandPsycho outwardly nice, extremely crazy inside]], and even more insane after normal Ryuko cuts off her arms.
*** Phlegmatic: [[TheDragon Rei]] -- The person able to serve at a moment's notice, and without question. However, she showcases the downsides by being an outwardly cold person. Exemplifies this part of her personality in the OVA by being a DefrostingIceQueen when she heard of Ragyo's death, and was able to be swayed by Satsuki.
*** Sanguine: Mind-Controlled Ryuko -- Instead of unable to express her emotions, she says them bluntly, taking them (sometimes literally) to the person she speaks to. Ryuko even kisses Nui, showcasing their love for each other. She balances out the other's negative emotions by being compassionate with them and follows after Ragyo by being '''''way''''' over-the-top in battles, but she showcases the negatives by being [[BrainwashedandCrazy mind-controlled]] into finally finding her wants of being with an actual family and comes off as harsh to others.
* ''Manga/TheLegendOfZelda'': The [[http://www.newgrounds.com/portal/view/328825 flash animations by Hadouken Dude]] took this to absolutely hilarious levels.
** Choleric: Red Link being the self-centered, temperamental and trouble-making {{Jerkass}}.
** Melancholic: Green Link being the fearless, determined and uptight leader.
** Phlegmatic: Blue Link being the analytical, cautious, and mildly snarky intellectual.
** Sanguine: Orchid Link being the childish, mischievous and clumsy noob.
* The Hinata House Girls in ''Manga/LoveHina'':
** Naru Narusegawa & Motoko Aoyama (choleric), Mitsune Konno (melancholic), Kaolla Su (sanguine), Shinobu Maehara (phlegmatic), and Mutsumi Otohime (eclectic).
* ''Anime/MacrossDelta''
** Delta Squadron:
*** Eclectic: Hayate Immelman. Brash, carefree rookie.
*** Sanguine: Chuck Mustang. Sociable, the "mood maker" of Delta.
*** Choleric: Mirage Farina Jenius. Mission focused, has a volatile attitude.
*** Melancholic: Messer Ihlefeld. The ace, has a serious demeanor.
*** Phlegmatic: Arad Mölders. The oldest member of the team as well as the leader of Delta.
** Walküre:
*** Eclectic: Freyja Wion. New member of the team, has a great love for singing
*** Sanguine: Makina Nakajima. Bubbly, carries an air of happiness around her.
*** Choleric: Mikumo Guynemer. The most action-oriented member of the team.
*** Melancholic: Reina Prowler. Stoic, quiet, close-minded.
*** Phlegmatic: Kaname Buccaneer. The leader, acts as the manages schedules and mission briefings.
* ''Anime/MagicalGirlLyricalNanohaStrikers''. natural leader (former) {{Tsundere}} Teana (choleric), knight-in-training Erio (melancholic), Caro (phlegmatic), and GenkiGirl [[ThePollyanna Pollyanna]] Subaru (sanguine). The last two's personalities actually caused a bit of family drama for their also melancholic adopted mother in the second [[AudioAdaptation StrikerS Sound Stage]], since [[NonverbalMiscommunication their combined introvertedness caused some communication problems]].
* ''Manga/MissKobayashisDragonMaid'': Kobayashi and the three main dragons.
** Melancholic: Kobayashi. Hard-working, introverted, and compassionate.
** Sanguine: Tohru. Hyperactive, cheerful, and reckless.
** Phlegmatic: Kanna. Stoic, sarcastic, and [[NoSocialSkills completely lacking in social skills]].
** Choleric: Ilulu. Spiteful, narrow-minded, and [[BloodKnight very confrontational]]... [[TookALevelInKindness at first]].
* The current lineup of the Taishiro in ''Manga/SamuraiDeeperKyo'' fits almost flawlessly.
** Sanguine: Yuan is extroverted, fond of wisecracks, and very protective of the people he cares about. He uses fire techniques and wears red.
** Choleric: Tokito is quick to anger, perfectionistic, and impulsive. Wears shades of yellow, and fights with a very aggressive energy.
** Melancholic: Hishigi is exceedingly introverted and the epitome of TheStoic, analytical and extremely task-focused. He shows textbook signs of severe depression. His color scheme is almost entirely black.
** Phlegmatic: Fubuki leads the Taishiro. He is level-headed, cultured and elegant. His fighting style revolves around water and his color scheme is mainly blue.
* ''Anime/{{Superbook}}'' (second season): Christopher Peepers (choleric), Joy (melancholic), Uriah (sanguine), and Gizmo (phlegmatic).
* The four main characters from ''Anime/{{Texhnolyze}}'': [[HotScientist Doc]] (sanguine), [[OnlySaneMan Onishi]] (choleric), [[TheStoic Ichise]] (phlegmatic), and [[WaifProphet Ran]] (melancholic).
* ''Manga/TheRoyalTutor'':
** The four main princes: [[{{Dandere}} Kai]] (phlegmatic), [[ThePerfectionist Bruno]] (melancholic), [[{{Tsundere}} Leonhard]] (choleric), and [[TheCharmer Licht]] (sanguine).
** Heine and the other members of the Granzreich royal family:
*** Phlegmatic: Heine. Stoic, intelligent, modest, and patient... [[BerserkButton for the most part]].
*** Melancholic: Viktor. Stern, rational, and cares strongly for his children.
*** Sanguine: Adele. Happy-go-lucky, childish, and playful.
*** Choleric: Eins. Unemotional, confident, and condescending.
* Five-temperament ensemble in ''Manga/TokyoMewMew'': At first [[IJustWantToBeNormal not happy about leading]], but accepting her responsibility later on, Ichigo (choleric); Minto (melancholic), Retasu (phlegmatic), the GenkiGirl Bu-ling (sanguine), and Zakuro (eclectic).
* ''Anime/TheTowerOfDruaga'':
** Jil's Group: Jil (melancholic), Ahmey (phlegmatic), Melt (choleric), Coopa (sanguine), and Kaaya (eclectic).
** Neeba's Group: Neeba (melancholic), Fatina (choleric), Kally (phlegmatic), and Utu (sanguine).
* ''Manga/{{Trigun}}'' more often averts this trope in favor of the [[TheDrifter Lone Hero]] or the PowerTrio. Adding Wolfwood to the cast has a subtractive effect instead, generating a [[RedOniBlueOni duo dynamic]] that leaves Meryl and Millie on the sidelines. When they participate equally they fit the four temperaments: Meryl (choleric), Vash (melancholic, though pretending to be sanguine), Wolfwood (phlegmatic), and Millie (sanguine).
* In the original ''Anime/WeissKreuz'' Aya (choleric), Omi (melancholic), Youji (phlegmatic), and Ken (sanguine).
* In ''Anime/WolfsRain'': Tsume (choleric), Kiba (melancholic), Toboe (phlegmatic), and Hige (sanguine).
* ''Manga/YourLieInApril'': Arima Kousei (melancholic), Miyazono Kaori (choleric), Watari Ryota (sanguine), Sawabe Tsubaki (phlegmatic).
* ''Anime/YuGiOh5Ds'': Jack (choleric), Yusei (melancholic), Akiza (phlegmatic), and Crow (sanguine).
* Out of the four "[[IdenticalStranger Yu's]]" from ''Anime/YugiohArcV'':
** Sanguine: Yuya aspires to make people smile and peacefully solve conflicts but can be [[MoodSwinger emotionally unstable]].
** Choleric: Yugo is hot-tempered and occasionally impulsive but is ambitious and protective of loved ones.
** Melancholic: Yuto is determined, pragmatic, and stoic, but has a self-blaming and cynical streak.
** Phlegmatic: Yuri is always level-headed and calm, even when relentlessly hunting people under orders of his leader, or [[ComicallySerious being a comedic plaything]].
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