Action Man: Alex (choleric), Rikki (melancholic), Grinder (phlegmatic), and Fidget (sanguine).
In Adventures of the Galaxy Rangers, The Series 5 Rangers: Zach (melancholic), Shane (choleric), Niko (phlegmatic), and Walter (sanguine). The series actually has very few episodes where all four are heavily involved, mostly breaking the team up into groups of two or three.
Aqua Teen Hunger Force: Master Shake (sanguine), Frylock (melancholic), Carl (choleric), and Meatwad (phlegmatic).
The Backyardigans: Pretty hard to qualify. Technically, all of them are sanguine, but Pablo is probably the most. As for the others: Tasha (choleric), Uniqua (melancholic, though not depressive, since we're talking about a little kids' show), and Tyrone (phlegmatic). Austin starts out as phlegmatic, then moving on to melancholic; these days, he's starting to become more eclectic.
The New Batman Adventures: Though they rarely all worked together in the same episode, there was still a four-person "bat family." Robin (Tim Drake) is sanguine; Nightwing (Dick Grayson) is choleric; Batman is melancholic (as if it had to be said; he basically defines the temperament); and Batgirl is phlegmatic. Catwoman was the back-up choleric; in one episode, she and Nightwing even team up because of their similar personalities.
And from Batman Beyond, we've got the Royal Flush Gang who also form a Five-Man Band with the robotic Ace (eclectic), authoritarian King (melancholic), conflicted Ten (choleric), Queen — a proper lady who tries to keep the family united (phlegmatic), and fun-loving Jack (sanguine).
Beast Wars: The four main Maximals: Optimus Primal (choleric), Rattrap (melancholic), Rhinox (phlegmatic), and Cheetor (sanguine).
The Dark Hand Enforcers (after Tohru left): Hak Foo (choleric), Chow (melancholic), Ratso (phlegmatic), and Finn (sanguine).
The heroes once Tohru joined them: Uncle (choleric), Jackie (melancholic), Tohru (phlegmatic), and Jade (sanguine).
Jelly Jamm: Bello (choleric), Goomo (phlegmatic), Mina (melancholic), Rita (sanguine), and Ongo (eclectic).
The Jetsons: George (sanguine), Jane (melancholic), Elroy (phlegmatic), and Judy (choleric) and Rosie (eclectic).
Jonny Quest: Jonny (melancholic), Hadji (sanguine), Dr Quest (phlegmatic), Race (choleric), and Bandit (eclectic).
The seven founding members of the Justice League seem to fit this pretty well for the most part: Superman (melancholic), Wonder Woman and Hawkgirl (choleric), Batman and Green Lantern (phlegmatic), Flash (sanguine), and Martian Manhunter (eclectic).
Kaijudo: Rise of the Duel Masters has three groups:
The Duel Masters: Nigel (choleric), Nadia (melancholic), Chavez (phlegmatic), Kimora (sanguine), and Jaha (eclectic).
The Choten's side: Alakshmi (choleric), Suguru/ Ken Okamoto (melancholic), The Choten (phlegmatic), Fingers (sanguine), and Heller (eclectic).
The four members of "Team Possible" in Kim Possible: Kim (melancholic), Ron (choleric), Rufus (sanguine), and Wade (phlegmatic).
King of the Hill: Hank Hill (melancholic), Dale Gribble (sanguine), Boomhauer (phlegmatic), and Bill Dauterive (choleric).
The Hill family also qualifies: Hank (melancholic), Peggy (choleric), Bobby (phlegmatic), and Luanne (sanguine).
The Land Before Time series: Littlefoot (melancholic), Cera (choleric), Petrie (sanguine), Ducky (phlegmatic), and Spike (eclectic).
Littlest Pet Shop (2012): Zoe and Pepper (choleric), Russell and Sunil (melancholic), Penny and Blythe (phlegmatic), Minka and Vinnie (sanguine).
The boys: Ralphie (sanguine), Carlos (choleric), Tim (melancholic), and Arnold (phlegmatic).
The girls: Wanda (sanguine), Keesha (choleric), Dorothy Ann (melancholic), and Phoebe (phlegmatic).
Men in Black: Jay (sanguine), Kay (melancholic), Elle (phlegmatic), and Zed (choleric).
Metalocalypse: Dethklok: Nathan (melancholic), Pickles (choleric), Skwisgaar (phlegmatic), Murderface (sanguine), and Toki (eclectic).
Moonbeam City: Dazzle is Melancholic, Pizzaz is Choleric, Chrysalis is Phlegmatic and Rad is Sanguine.
Mr. Bogus: In one of the rare cases that Bogus and his cousin Brattus get paired up with their enemies, Ratty and Mole, it goes like this: Bogus (melancholic), Brattus (sanguine) Ratty (choleric), and Mole (phlegmatic).
Oscars Oasis: On the rare ocassions Oscar teams up with the trio: Popy (choleric), Buck (melancholic), Oscar (phlegmatic), and Harchi (sanguine).
The Perils of Penelope Pitstop: Penelope is melancholic; Clyde is choleric; Dum-Dum and Pockets are phlegmatic; and the Hooded Claw (Sylvester Sneekly) is sanguine. Of the other characters: Softy (melancholic), Yak-Yak (sanguine), Snoozy (phlegmatic), and Zippy (eclectic).
Transformers has the Dinobots. Grimlock would be choleric when compared to almost any other Autobot, but amongst his own team he's relegated to leukine, with violent, sociopathic Slag as the resident choleric. The other three are fairly cut-and-dried, with pessimist Snarl as the melancholic, quiet and somewhat dim Sludge as the phlegmatic, and affable Swoop as the sanguine.
The five main Autobots of Transformers Animated: Optimus Prime (choleric), Ratchet (melancholic), Prowl (phlegmatic), Bumblebee (sanguine), and Bulkhead (eclectic).