Action Man (2000): Alex (choleric), Rikki (melancholic), Grinder (phlegmatic), and Fidget (sanguine).
In Adventures of the Galaxy Rangers, The Series 5 Rangers: Zach (melancholic), Shane (choleric), Niko (phlegmatic), and Walter (sanguine). The series actually has very few episodes where all four are heavily involved, mostly breaking the team up into groups of two or three.
The Air Nomads (sanguine): Relied on kindness, peace and each other mostly, they lacked any real goal besides meditation and teachings which incidentally was part of the reason how they got wiped out.
The Fire Nation (choleric): Viewed power, military prowess and pride as success, many were either corrupt, cruel or ruthless in nature. Instigating wars and showing undying loyalty towards the Fire Lord was all they truly cared about.
The Earth Kingdom (melancholic): Supported hard work and manual labour along with a heavily structured society and traditions. Their belief in politics is very strong and law is absolute to them no matter how dodgy.
The Water Tribe (phlegmatic): Cherished families and allies despite being the most remote nation. Though split into Southern and Northern sibling tribes, as a whole both seek a serene and fairly simple existence surviving off the land.
Aqua Teen Hunger Force: Master Shake (sanguine), Frylock (melancholic), Carl (choleric), and Meatwad (phlegmatic).
The Backyardigans: Pretty hard to qualify. Technically, all of them are sanguine, but Pablo is probably the most. As for the others: Tasha (choleric), Uniqua (melancholic, though not depressive, since we're talking about a little kids' show), and Tyrone (phlegmatic). Austin starts out as phlegmatic, then moving on to melancholic; these days, he's starting to become more eclectic.
The New Batman Adventures: Though they rarely all worked together in the same episode, there was still a four-person "bat family." Robin (Tim Drake) is sanguine; Nightwing (Dick Grayson) is choleric; Batman is melancholic (as if it had to be said; he basically defines the temperament); and Batgirl is phlegmatic. Catwoman was the back-up choleric; in one episode, she and Nightwing even team up because of their similar personalities.
And from Batman Beyond, we've got the Royal Flush Gang who also form a Five-Man Band with the robotic Ace (eclectic), authoritarian King (melancholic), conflicted Ten (choleric), Queen — a proper lady who tries to keep the family united (phlegmatic), and fun-loving Jack (sanguine).
Beast Wars: The four main Maximals: Optimus Primal (choleric), Rattrap (melancholic), Rhinox (phlegmatic), and Cheetor (sanguine).
Tedd, the calm and soft-spoken alien (phlegmatic).
Jimmy, the hyper alien who has the most humane of solutions (sanguine).
Sammy, seems to be the leader, as well as the more twisted, of the extraterrestrial trio (choleric).
And Jeff himself, a somewhat neurotic mannote Which really says a lot about Earth if he is its "most average guy." whose life gets even more miserable ever since the aliens started sharing his apartment with him (melancholic).
Jelly Jamm: Bello (choleric), Goomo (phlegmatic), Mina (melancholic), Rita (sanguine), and Ongo (eclectic).
The Jetsons: George (sanguine), Jane (melancholic), Elroy (phlegmatic), and Judy (choleric) and Rosie (eclectic).
Jonny Quest: Jonny (melancholic), Hadji (sanguine), Dr Quest (phlegmatic), Race (choleric), and Bandit (eclectic).
The seven founding members of the Justice League seem to fit this pretty well for the most part: Superman (melancholic), Wonder Woman and Hawkgirl (choleric), Batman and Green Lantern (phlegmatic), Flash (sanguine), and Martian Manhunter (eclectic).
Kaeloo: Kaeloo (sanguine), Stumpy (choleric), Quack Quack (phlegmatic) and Mr. Cat (melancholic).
Kaijudo: Rise of the Duel Masters has three groups:
The Duel Masters: Nigel (choleric), Nadia (melancholic), Chavez (phlegmatic), Kimora (sanguine), and Jaha (eclectic).
The Choten's side: Alakshmi (choleric), Suguru/ Ken Okamoto (melancholic), The Choten (phlegmatic), Fingers (sanguine), and Heller (eclectic).
The four members of "Team Possible" in Kim Possible: Kim (melancholic), Ron (choleric), Rufus (sanguine), and Wade (phlegmatic).
King of the Hill: Hank Hill (melancholic), Dale Gribble (sanguine), Boomhauer (phlegmatic), and Bill Dauterive (choleric).
The Hill family also qualifies: Hank (melancholic), Peggy (choleric), Bobby (phlegmatic), and Luanne (sanguine).
Kuu Kuu Harajuku: Love is melancholic; Angel is phlegmatic; Music is choleric; and Baby is sanguine. Team leader G is their eclectic.
The Land Before Time series: Littlefoot (melancholic), Cera (choleric), Petrie (sanguine), Ducky (phlegmatic), and Spike (eclectic).
The boys: Ralphie (sanguine), Carlos (choleric), Tim (melancholic), and Arnold (phlegmatic).
The girls: Wanda (sanguine), Keesha (choleric), Dorothy Ann (melancholic), and Phoebe (phlegmatic).
Men in Black: Jay (sanguine), Kay (melancholic), Elle (phlegmatic), and Zed (choleric).
Metalocalypse: Dethklok: Nathan (melancholic), Pickles (choleric), Skwisgaar (phlegmatic), Murderface (sanguine), and Toki (eclectic).
Moonbeam City: Dazzle is Melancholic, Pizzaz is Choleric, Chrysalis is Phlegmatic and Rad is Sanguine.
Mr. Bogus: In one of the rare cases that Bogus and his cousin Brattus get paired up with their enemies, Ratty and Mole, it goes like this: Bogus (melancholic), Brattus (sanguine) Ratty (choleric), and Mole (phlegmatic).
The Perils of Penelope Pitstop: Penelope is melancholic; Clyde is choleric; Dum-Dum and Pockets are phlegmatic; and the Hooded Claw (Sylvester Sneekly) is sanguine. Of the other characters: Softy (melancholic), Yak-Yak (sanguine), Snoozy (phlegmatic), and Zippy (eclectic).
Transformers has the Dinobots. Grimlock would be choleric when compared to almost any other Autobot, but amongst his own team he's relegated to leukine, with violent, sociopathic Slag as the resident choleric. The other three are fairly cut-and-dried, with pessimist Snarl as the melancholic, quiet and somewhat dim Sludge as the phlegmatic, and affable Swoop as the sanguine.
The five main Autobots of Transformers Animated: Optimus Prime (choleric), Ratchet (melancholic), Prowl (phlegmatic), Bumblebee (sanguine), and Bulkhead (eclectic).