* ''WesternAnimation/ActionMan'': Alex (choleric), Rikki (melancholic), Grinder (phlegmatic), and Fidget (sanguine).
* From ''WesternAnimation/AdventuresFromTheBookOfVirtues'', Plato (phlegmatic), Aristotle (melancholic), Socrates (choleric), Aurora (sanguine), and Zack and Annie (both leukine).
* ''WesternAnimation/AdventureTime'': Finn (melancholic), Jake (sanguine), Princess Bubblegum (phlegmatic), Marceline & Ice King (choleric) and BMO (leukine).
* In ''WesternAnimation/AdventuresOfTheGalaxyRangers'', The Series 5 Rangers; Zach (melancholic), Shane (choleric), Niko (phlegmatic), and Walter (sanguine). The series actually has very few episodes where all four are heavily involved, mostly breaking the team up into groups of two or three.
* ''Franchise/AlvinAndTheChipmunks'': Dave (choleric), [[TheSmartGuy Simon]] (melancholic), [[TheFace Alvin]] (sanguine), and Theodore (phlegmatic).
* ''WesternAnimation/TheAmazingChanAndTheChanClan'', two examples.
** Four youngest children: Flip (choleric), Mimi (melancholic), Nancy (phlegmatic), and Scooter (sanguine).
** The middle four: Anne (choleric), Alan (sanguine), Tom (melancholic), and Suzie (phlegmatic).
** Mr. Charlie and the two oldest: Mr. Charlie (leukine), Henry (choleric/melancholic), and Stanley (sanguine).
* ''TheAmazingSpiez'': Lee (choleric), Marc (melancholic), Megan (phlegmatic), and Tony (sanguine). Megan and Marc sometimes switch roles.
* [[FanNickname Ozai's Angels]] ([[MyFriendsAndZoidberg and]] [[TheOneGuy Zuko]]) of ''WesternAnimation/AvatarTheLastAirbender'': [[TheChessmaster Azula]] (choleric), [[ByronicHero Zuko]] (melancholic, who pretends to be choleric), [[TheQuietOne Mai]] (phlegmatic), and [[{{Cloudcuckoolander}} Ty Lee]] (sanguine).
** The [[FanNickname Gaang]] themselves become one of these as well during the second season: Toph (choleric), Sokka (sanguine), Aang (melancholic, though still sanguine in many ways), and Katara (phlegmatic).
** The Avatars before Aang also fit: Kyoshi (choleric), Yangchen (melancholic), Roku (phlegmatic), and Kuruk (sanguine).
** ''WesternAnimation/TheLegendOfKorra'' follows this perfectly: [[HotBlooded Korra]] (choleric), [[TallDarkAndHandsome Mako]] (melancholic), [[UptownGirl Asami]] (phlegmatic), [[TheCharmer Bolin]] (sanguine), and [[TheMentor Tenzin]] (leukine).
*** The Villains: Amon (phlegmatic), Vaatu (choleric), Unalaq (melancholic), Zaheer (an interesting melancholic/sanguine), Kuvira (choleric/melancholic).
*** Tenzin's Children (with the exception of Rohan): Jinora (melancholic), Ikki (sanguine), Meelo (choleric).
*** Suyin's Children: Baatar Jr (choleric), Huan (melancholic), Opal (phlegmatic), Wei and Wing (sanguine/leukine).
* ''WesternAnimation/TheBackyardigans'': Pretty hard to qualify. Technically, all of them are sanguine, but Pablo is probably the most. As for the others: Tasha (choleric), Uniqua (melancholic, though not depressive, since we're talking about a little kids' show), and Tyrone (phlegmatic). Austin starts out as phlegmatic, then moving on to melancholic; these days, he's starting to become more leukine.
* ''[[WesternAnimation/BatmanTheAnimatedSeries The New Batman Adventures]]'': Though they rarely all worked together in the same episode, there was still a four-person "bat family." Robin (Tim Drake) is sanguine; Nightwing (Dick Grayson) is choleric; Batman is melancholic (as if it had to be said; he basically defines the temperament); and Batgirl is phlegmatic. Catwoman was the back-up choleric; in one episode, she and Nightwing even team up because of their similar personalities.
* And from ''WesternAnimation/BatmanBeyond'', we've got the Royal Flush Gang who also form a [[FiveManBand Five Man Band]] with the robotic Ace (luekine), Authoritarian King (melancholic), conflicted Ten (choleric), Queen -- a proper lady who tries to keep the family united (phlegmatic), and fun-loving Jack (sanguine).
* ''WesternAnimation/BeastWars'': The four main Maximals: Optimus Primal (choleric), Rattrap (melancholic), Rhinox (phlegmatic), and Cheetor (sanguine).
* ''WesternAnimation/BeavisAndButthead'': Beavis (sanguine), Butt-Head (choleric), WesternAnimation/{{Daria}} (melancholic), and Stewart (phlegmatic).
* ''WesternAnimation/BlinkyBill'': Blinky Bill and Marcia Marsupial Mouse (choleric), Nutsy Koala (melancholic), Flap Platypus (phlegmatic), Splodge Kangaroo (sanguine), and Shifty Dingo (leukine).
* ''WesternAnimation/BobsBurgers'': Bob (melancholic), Linda and Gene (sanguine), Tina (phlegmatic), Louise (choleric)
* ''WesternAnimation/{{Bonkers}}'': Lucky Piquel (choleric), Fawn Deer (melancholic), Fallapart Rabbit (phlegmatic), and Bonkers (sanguine).
* ''WesternAnimation/TheBoondocks'': Robert (choleric), Huey (melancholic), Tom (phlegmatic), Riley (sanguine), and Jazmine (leukine).
* ''WesternAnimation/{{Brickleberry}}'': Steve (melancholic), Denzel and Woody (choleric), Ethel (phlegmatic), Connie (sanguine), and Malloy (leukine).
* ''WesternAnimation/CaptainPlanetAndThePlaneteers'': Kwame (melancholic - earth), Wheeler (choleric - fire), Linka (sanguine - wind / air), Gi (phlegmatic - water), and Ma-ti (leukine - heart).
* ''WesternAnimation/CodeLyoko'': [[BirdsOfAFeather Yumi and Ulrich]] (melancholic), Jeremie (phlegmatic), Odd (sanguine), and Aelita (leukine).
* ''WesternAnimation/CodenameKidsNextDoor'': Numbuh One - Nigel Uno (melancholic), Numbuh Two - Hoagie P. Gilligan Jr. (phlegmatic), Numbuh Three - Kuki Sanban (sanguine), Numbuh Four - Wallabee Beetles (choleric), and Numbuh Five - Abigail Lincoln (leukine).
* ''WesternAnimation/TheDaltons'' has Joe (choleric), Jack (phlegmatic), William (melancholic), and Averell (sanguine).
* ''WesternAnimation/DanVs'': Dan (choleric), Elyse Jr. (melancholic), Chris (phlegmatic), Mr. Mumbles (sanguine)
* ''WesternAnimation/DannyPhantom'''s four-member "Team Phantom" can exhibit these: Danny is Choleric for good leadership skills, but is highly emotional; Jazz (though not depressive) is Melancholic for her kind-but-perfectionist persona; Sam is phlegmatic because she's a responsible [[DeadpanSnarker Stoic Goth]]; and Tucker is Sanguine for he's fun loving.
* ''WesternAnimation/{{Daria}}'':
** The Fashion Club: Quinn (melancholic), Sandi (choleric), Stacy (sanguine), and Tiffany (phlegmatic).
** Daria's family: Quinn (sanguine), Helen (choleric), Daria (melancholic), and Jake (phlegmatic).
** As far as Lawndale High School: Mr. [=DeMartino=] (choleric), Mr. O'Neill (phlegmatic), Kevin and Brittany (sanguine), and Mack and Jodie (melancholic)
** Finally, there's [[FakeBand Mystik Spiral]]: Trent Lane (phlegmatic), Jesse Moreno (melancholic), Max Tyler (choleric), and Nicholas Campbell (sanguine).
* ''WesternAnimation/DastardlyAndMuttleyInTheirFlyingMachines'': Dick Dastardly (choleric), Klunk (melancholic), and Zilly (phlegmatic), and Muttley (sanguine).
* The family of ''WesternAnimation/DextersLaboratory'': Dexter (melancholic), Dee Dee (choleric), Mom (phlegmatic), and Dad (sanguine).
* The four main kids in ''WesternAnimation/{{Doug}}'': Doug (melancholic), Skeeter (sanguine), Patti (phlegmatic), and Beebe (choleric).
* The neighborhood kids on ''WesternAnimation/EdEddNEddy'':: Sarah and Kevin (choleric), Jimmy (melancholic), Jonny (sanguine), and Plank (leukine), Rolf, and Nazz (phlegmatic).
* ''WesternAnimation/FairlyOddParents'': Timmy (melancholic), Cosmo (sanguine), Wanda (phlegmatic), Poof (choleric), and Sparky (leukine).
* ''WesternAnimation/FamilyGuy'':
** Neighbors: Peter (choleric), Joe (melancholic), Cleveland (phlegmatic), and Quagmire (sanguine).
** Family: Peter and Stewie (choleric), Lois (phlegmatic), Meg and Brian (melancholic), and Chris (sanguine).
* ''WesternAnimation/TheFlintstones'': Fred (choleric), Betty (melancholic), Wilma (phlegmatic), and Barney (sanguine).
* ''WesternAnimation/{{Futurama}}'': Fry (melancholic), Leela (choleric), Bender (sanguine), and Farnsworth (phlegmatic).
** Other characters: Zoidberg (sanguine), Hermes (phlegmatic), Nibbler (melancholic), Amy (choleric), and Scruffy (leukine).
* ''WesternAnimation/{{Gargoyles}}'': Goliath (melancholic), Brooklyn (choleric), Broadway (sanguine), Lexington (phlegmatic), and Hudson/Bronx (leukine)
* ''WesternAnimation/GoofTroop'': Max (sanguine), Pete (choleric), P.J. (melancholic), and Goofy (phlegmatic).
* ''WesternAnimation/GravityFalls'': Grunkle Stan (choleric), Mabel (sanguine), Dipper (melancholic), Soos (phlegmatic) and Wendy (leukine).
* ''WesternAnimation/GreenLanternTheAnimatedSeries'': [[CowboyCop Hal Jordan]] (sanguine), [[DrillSergeantNasty Kilowog]] (choleric), [[SpaceshipGirl Aya]] (phlegmatic) and [[TheAtoner Razer]] (melancholic).
* ''WesternAnimation/{{Grojband}}'': Corey (sanguine), Laney (phlegmatic), Kin (melancholic), and Kon (choleric).
** Also, Trina (choleric), Mina (melancholic), Kate and Allie (sanguines), and Nick (phlegmatic).
* A good chunk of the classic ''Creator/HannaBarbera'' cartoon characters that are connected to the Yogi Bear universe fit this mold if you put them together (think of all of the main characters in ''WesternAnimation/YogisTreasureHunt'' minus Ranger Smith, Snooper and Blabbermouse, Auggie Doggie and Doggie Daddy with Baba Looey included). Yogi (sanguine), Quick Draw [=McGraw=] (choleric, albeit a more dim-witted and incompetent example), Snagglepuss (melancholic, although mostly in terms of mannerisms since he's much more upbeat and cheerful than most examples), Huckleberry Hound (phlegmatic), Boo Boo and Baba Looey (leukine), and Top Cat (sanguine/choleric).
* The four main kids on ''WesternAnimation/HeyArnold'': Arnold (melancholic), Helga (choleric), Gerald (sanguine), and Phoebe (phlegmatic).
* ''WesternAnimation/InvaderZim'': Zim (choleric), Dib (melancholic), Gaz (phlegmatic), and Gir (sanguine).
* ''WesternAnimation/JackieChanAdventures'':
** The Dark Hand Enforcers ([[HeelFaceTurn after Tohru left]]): Hak Foo (choleric), Chow (melancholic), Ratso (phlegmatic), and Finn (sanguine).
** The heroes once Tohru joined them: Uncle (choleric), Jackie (melancholic), Tohru (phlegmatic), and Jade (sanguine).
* ''WesternAnimation/TheJetsons'': George (sanguine), Jane (melancholic), Elroy (phlegmatic), and Judy (choleric) and Rosie (leukine).
* WesternAnimation/JonnyQuest: Jonny (melancholic), Hadji (sanguine), Dr Quest (phlegmatic), Race (choleric), and Bandit (leukine)
* The seven founding members of the ''WesternAnimation/JusticeLeague'' seem to fit this pretty well for the most part: Superman (melancholic), Wonder Woman and Hawkgirl (choleric), Batman and Green Lantern (phlegmatic), Flash (sanguine), and Martian Manhunter (leukine).
* ''WesternAnimation/{{Kaijudo}}: Rise of the Duel Masters'' has three groups:
** The main trio: Ray (choleric/sanguine), Gabe (melancholic), and Allie (sanguine).
** The Duel Masters: Nigel (choleric), Nadia (melancholic), Chavez (phlegmatic), Kimora (sanguine), and Jaha (leukine).
** The Choten's side: Alakshmi (choleric), Suguru/[[spoiler: Ken Okamoto]] (melancholic), The Choten (phlegmatic), Fingers (sanguine), and Heller (leukine).
* The four members of "Team Possible" in ''WesternAnimation/KimPossible'': Kim (melancholic), Ron (choleric), Rufus (sanguine), and Wade (phlegmatic).
* ''WesternAnimation/KingOfTheHill'': Hank Hill (melancholic), Dale Gribble (choleric), Boomhauer (phlegmatic), and Bill Dauterive (sanguine).
** The Hill family also qualifies: Hank (melancholic), Peggy (choleric), Bobby (phlegmatic), and Luanne (sanguine).
* ''WesternAnimation/TheLandBeforeTime'' series: Littlefoot (melancholic), Cera (choleric), Petrie (sanguine), Ducky (phlegmatic), and Spike (leukine).
* ''WesternAnimation/LittlestPetShop2012'': Zoe and Pepper (choleric), Russell and Sunil (melancholic), Penny and Blythe (phlegmatic), Minka and Vinnie (sanguine).
* ''WesternAnimation/TheLooneyTunesShow'': Bugs Bunny (melancholic/phlegmatic), Daffy Duck (choleric), Lola Bunny (sanguine/choleric), Porky Pig (phlegmatic/leukine) and Tina Russo Duck (leukine/phlegmatic).
* The kids in Ms. Frizzle's class on ''Literature/TheMagicSchoolBus'':
** The boys: Ralphie (sanguine), Carlos (choleric), Tim (melancholic), and Arnold (phlegmatic).
** The girls: Wanda (sanguine), Keesha (choleric), Dorothy Ann (melancholic), and Phoebe (phlegmatic).
* ''WesternAnimation/MenInBlack'': Jay (sanguine), Kay (melancholic), Elle (phlegmatic), and Zed (choleric).
* ''WesternAnimation/{{Metalocalypse}}'': Dethklok: Nathan (melancholic), Pickles (choleric), Skwisgaar (phlegmatic), Murderface (sanguine) and Toki (leukine).
* ''WesternAnimation/MrBogus'': In one of the rare cases that Bogus and his cousin Brattus get paired up with their enemies, Ratty and Mole, it goes like this: Bogus (melancholic), Brattus (sanguine) Ratty (choleric), and Mole (phlegmatic).
* ''WesternAnimation/MyLittlePonyFriendshipIsMagic'':
** Choleric: Applejack (extroverted, confident, and stubborn) and Rainbow Dash (ambitious and prone to aggression).
** Melancholic: Twilight Sparkle (introverted, organized, and has high expectations) and Rarity (work-focused and obsessed with detail).
** Phlegmatic: Fluttershy (introverted, compassionate, and docile).
** Sanguine: Pinkie Pie (fun-loving, loud, and driven to seek others' attention).
** Leukine: Spike (the most well-balanced of the seven).
** Princess Luna (melancholic), Princess Celestia (leukine), and Princess Cadance (sanguine).
*** Works for the four main villains, too: Queen Chrysalis (choleric), Nightmare Moon (melancholic), King Sombra (phlegmatic), and Discord (''definitely'' sanguine).
*** It works for Cutie Mark Crusaders too: Apple Bloom (sanguine), Sweetie Belle (phlegmatic/melancholic) and Scootaloo (choleric)
* ''WesternAnimation/OscarsOasis'': On the rare ocassions Oscar teams up with the trio: Popy (choleric), Buck (melancholic), Oscar (phlegmatic), and Harchi (sanguine).
* ''WesternAnimation/ThePenguinsOfMadagascar'': Skipper (melancholic), Kowalski (phlegmatic), Private (sanguine), and Rico (choleric).
* ''WesternAnimation/ThePerilsOfPenelopePitstop'': Penelope is melancholic; Clyde is choleric; Dum-Dum and Pockets are phlegmatic; and the Hooded Claw (Sylvester Sneekly) is sanguine. Of the other characters: Softy (melancholic), Yak-Yak (sanguine), Snoozy (phlegmatic), and Zippy (leukine).
* ''WesternAnimation/{{Phineas and Ferb}}''
** Fletcher family: [[{{Adorkable}} Lawrence Fletcher]] (melancholic), [[StraightMan Linda Flynn-Fletcher]] (sanguine), [[FieryRedhead Candace Flynn-Fletcher]] (choleric), [[TheAce Phineas Flynn-Fletcher]] (sanguine) and [[TheQuietOne Ferb Fletcher]] (phlegmatic)
** The Johnson family: Jack Johnson (sanguine), "Hawkeye" Johnson (melancholic), Jeremy Johnson (phlegmatic), Suzy Johnson (choleric)
** The kids: Baljett Tjinder (melancholic), Buford van Stomm (choleric), Isabella Garcia Shapiro (phlegmatic), Irving Du Bois (leukine)
** The teens: Stacy Hirano (sanguine), Jenny Brown and Vanessa Doofenshmirtz (melancholic), Coltrane and Monty Monogram (phlegmatic), Albert Du Bois (choleric)
** OWCA: Major Francis Monogram (choleric), Carl Karl and Perry the Platypus (leukine)
** Other: Dr. Heinz Doofenshmirtz (sanguine/choleric), Charlene Doofenshmirtz (melancholic)
* ''WesternAnimation/PigGoatBananaCricket'': Pig (phlegmatic), Goat (choleric), Banana (sanguine), and Cricket (melancholic).
* ''WesternAnimation/ThePowerpuffGirls'': Bubbles (sanguine), Buttercup (choleric), Blossom (melancholic), Professor Utonium (phlegmatic)
* ''WesternAnimation/{{Recess}}'':
** Melancholic: T.J. Detweiler (Team leader, determined, charismatic).
** Choleric: ([[DoNotCallMePaul Ashley]]) Spinelli (Hot-tempered, ambitious).
** Choleric/Melancholic: Vince [=LaSalle=] (Hard-working perfectionist who's also ambitious and determined).
** Phlegmatic: Gretchen Grundler (Smart, hard-working).
** Sanguine: Mikey Blumberg (Extroverted, optimistic).
** Leukine: Gus Griswald (Sweet, shy).
* ''WesternAnimation/RegularShow'' generally has two characters per temperament for the main park staff:
** Sanguine: [[ManChild Rigby]] (also choleric) and [[{{Cloudcuckoolander}} Pops]] (also phlegmatic)
** Choleric: [[HairTriggerTemper Benson]] (also melancholic) and [[ThePrankster Muscle Man]] (also sanguine)
** Phlegmatic: [[MrFixit Skips]] --[[spoiler:formerly [[ByronicHero Walks]]]]-- (also melancholic) and [[TheQuietOne Hi-Five Ghost]] (also leukine)
** Melancholic: [[CoolLoser Mordecai]] (also phlegmatic)
** Leukine: [[NaiveNewcomer Thomas]]
* ''WesternAnimation/RocketPower'': Otto (choleric), Reggie (melancholic), Twister (sanguine), and Sam/Squid (phlegmatic).
* ''WesternAnimation/RockosModernLife'': Heffer (choleric), Filburt (phlegmatic/melancholic), Rocko (melancholic/phlegmatic), and Spunky (sanguine).
* ''WesternAnimation/{{Rugrats}}'':
** The babies: Tommy (melancholic), Chuckie (phlegmatic), Phil & Lil (sanguine), Angelica (choleric), and Spike/Dil (leukine)
* The human characters of ''WesternAnimation/ScoobyDooMysteryIncorporated'' appropriately [[ColourCodedForYourConvenience match their own color schemes]]. Daphne (Red Hair) is choleric/sanguine, the most enthusiastic, emotional and outspoken of the group. Velma (Yellow Sweater) is phlegmatic/melancholic, exhibiting brains when solving the mystery, however she gets rather pushy, and tends to brood when things don't go her way. Fred (Blue Trousers) is melancholic/choleric as he is the leader of the team, obsessive (when it comes to traps) and emotionally high strung (when Daphne is in danger). Shaggy (Green Shirt) is sanguine/phlegmatic, the NonActionGuy who runs from anything spooky, is only there to react to the situations and makes jokes. Scooby (Brown Fur) fits into leukine, as he is rather non-confrontational and the heart of the team.
** The main villains, as well: Mayor Jones (Choleric), Professor Pericles (Phlegmatic), Mr. E (Melancholic), and Brad & Judy (sanguine).
* ''WesternAnimation/TheSimpsons'' have varied personalities over the years, but here is the most traditional lineup:
** Homer (choleric/sanguine), Marge (phlegmatic), Bart (sanguine/choleric), Lisa (melancholic), Maggie (leukine) and Grandpa "Abe" Simpson (choleric).
** Bart's "gang"/Party Posse: Bart is melancholic (although a rather outgoing one), Milhouse is phlegmatic, Nelson is choleric, and Martin/Ralph is sanguine.
** The regulars at Moe's Tavern: Homer (choleric), Barney (sanguine), Carl (melancholic), Lenny (phlegmatic), and Moe (leukine).
** The Bullies: Nelson (melancholic), Jimbo (choleric), Dolph (phlegmatic), and Kearney (sanguine).
* ''WesternAnimation/SouthPark:'' [[UnsympatheticComedyProtagonist C]][[BrilliantButLazy a]][[ManipulativeBastard rt]][[DangerouslyGenreSavvy man]] (choleric), [[TheHedonist Ke]][[ButtMonkey nny]] (sanguine), [[InsufferableGenius Ky]][[DeadpanSnarker le]] (phlegmatic), [[OnlySaneMan St]][[TheEveryman an]] (melancholic), and [[ChildrenAreInnocent Bu]][[PollyAnna tt]][[GotVolunteered e]][[KiddieKid rs]] (leukine).
** The Marshes: Randy (sanguine), Sharon (phlegmatic), Stan (melancholic), and Shelly (choleric).
* ''SpongebobSquarepants'': [[CloudCuckooLander Spongebob]] (melancholic/sanguine), [[TheDitz Patrick]] (sanguine), [[DeadpanSnarker Squidward]] (phlegmatic/melancholic), [[{{Greed}} Mr. Krabs]] (choleric), [[GeniusBruiser Sandy]] (leukine/choleric) and [[RidiculouslyCuteCritter Gary]] (leukine).
* ''WesternAnimation/StevenUniverse'': Steven (sanguine), Garnet (phlegmatic), Amethyst (choleric) and Pearl (melancholic).
* The teachers from ''WesternAnimation/StickinAround'': Mr. Lederhosen (choleric), Principal Coffin (melancholic/phlegmatic), and Ms. Mobley (sanguine).
* ''WesternAnimation/StormHawks'': Aerrow (choleric), Stork (melancholic), Junko (phlegmatic), Finn (sanguine), and Piper (leukine).
** The Cyclonians, as well: Ravess (choleric), Master Cyclonis (melancholic), Dark Ace (phlegmatic), and Snipe (sanguine).
* ''WesternAnimation/TaleSpin'': Rebecca (choleric), Kit (melancholic), Wildcat (phlegmatic), Baloo (sanguine), and Molly (leukine).
* To quote the ''WesternAnimation/{{Teenage Mutant Ninja Turtles|1987}}'' theme song: Leonardo leads (melancholic); Donatello does machines (phlegmatic); Raphael is cool, but rude (choleric); Michaelangelo is a party dude (sanguine).
** Or, for ''WesternAnimation/TeenageMutantNinjaTurtles2003'' (The same combination as above), "Leonardo's always in control; The wiseguy is Michelangelo; Donatello, he's the brains of the bunch; Count on Raphael to throw the first punch!"
*** The Ninja Turtles must ''really love'' combining this trope with their ThemeTuneRollCall, because ''they did it again'' for the ''WesternAnimation/TeenageMutantNinjaTurtles2012'' theme song! (The same combination as the first one) "Leonardo is the leader in blue! He does anything it takes to get his ninjas through. Donatello is the fellow has a way with machines. Raphael's got the most attitude on the team! Michelangelo! He's one of a kind and you know just where to find him when it's party time! Master Splinter taught them every single skill they need to be one lean, mean, green, incredible team!"
* ''WesternAnimation/TeenTitans'' and ''WesternAnimation/TeenTitansGo'': Robin (melancholic), Beast Boy (sanguine), Cyborg (choleric), Raven (phlegmatic), and Starfire (leukine).
** And as in the comics, the Doom Patrol with Mento replacing Niles Caulder as the choleric leader.
* The tender engines in ''ThomasTheTankEngine'': Gordon (choleric), Henry (melancholic), Edward (phlegmatic), and James (sanguine).
* ''WesternAnimation/ThunderCats2011'':
** Lion-O (melancholic), Pumyra (choleric), Panthro (starts out melancholic, gradually becomes more choleric), Cheetara (phlegmatic), Wilykit (phlegmatic/melancholic), Tygra (sanguine), and Wilykat (leukine).
** For the villains: Slithe (choleric), Vultaire (melancholic), Addicus (phlegmatic), Kaynar (sanguine), and Mumm-Ra (leukine).
** In the [[ThunderCats original series]], Tygra was the melancholic one while Panthro was choleric. Kit and Kat's temperaments were also reversed.
* ''WesternAnimation/TinyToonAdventures'': Babs (choleric), Buster (melancholic), Plucky (sanguine), and Hampton (phlegmatic).
* ''WesternAnimation/TitanMaximum'': Palmer (choleric), Sasha (sanguine), Jodi (phlegmatic), Willie (melancholic), and Leon (leukine).
** Originally, Palmer (choleric), Sasha (sanguine), Jodi (phlegmatic), Gibbs (melancholic), and Spud (leukine).
* ''Franchise/{{Transformers}}'' has the Dinobots. [[TheLeader Grimlock]] would be choleric when compared to almost any other Autobot, but amongst his own team he's relegated to leukine, with violent, sociopathic [[TheLancer Slag]] as the resident choleric. The other three are fairly cut-and-dried, with pessimist Snarl as the melancholic, quiet and somewhat dim Sludge as the phlegmatic, and affable Swoop as the sanguine.
* The five main Autobots of ''WesternAnimation/TransformersAnimated'': Optimus Prime (choleric), Ratchet (melancholic), Prowl (phlegmatic), Bumblebee (sanguine), and Bulkhead (leukine).
* ''WesternAnimation/TransformersPrime'':
** Team Prime: Arcee and Ultra Magnus (choleric), Ratchet (melancholic), Bumblebee (phlegmatic), Bulkhead, Wheeljack and Smokescreen (sanguine), and Optimus (leukine).
** The Decepticons: Megatron and Starscream (choleric), Dreadwing and Predaking (melancholic), Soundwave (phlegmatic), Knock Out (sanguine), Breakdown and Shockwave (leukine).
* ''WesternAnimation/TransformersRescueBots'':
** The Rescue Bots are: Heatwave (melancholic), Chase (choleric), Boulder (sanguine), and Blades (phlegmatic).
* ''WesternAnimation/{{Trollz}}'': Ruby (Choleric), Amethyst/Onyx (Melancholic), Topaz (Sanguine), Sapphire (Phlegmatic).
* WesternAnimation/UltimateSpiderMan: Power Man (melancholic), Iron Fist (phlegmatic), White Tiger (choleric), Nova (sanguine), and Spider-Man (leukine)
* UncleGrandpa: Uncle Grandpa (Melancholic), Giant Realistic Flying Tiger (Choleric), Mr. Gus (Phlegmatic), Pizza Steve (Sanguine) and Belly Bag (Leukine).
* Most of the main characters of ''WesternAnimation/{{VeggieTales}}'' fit this mold; [[OnlySaneMan Bob the Tomato]] (choleric), [[{{Cloudcuckoolander}} Larry the Cucumber]] (sanguine), [[CheerfulChild Junior Asparagus]] (leukine), [[ThePerfectionist Archibald Asparagus]] (melancholic), [[LargeHam Jimmy Gourd]] (sanguine/choleric), and [[TheQuietOne Jerry Gourd]] (phlegmatic).
* ''WesternAnimation/TheVentureBrothers'': Brock Samson (choleric, sometimes melancholic), Dean (phlegmatic), Dr. Venture (melancholic, sometimes choleric), and Hank (sanguine).
* ''WanderOverYonder'': Wander (sanguine/phlegmatic), Sylvia (sanguine/choleric), Lord Hater (choleric), Peepers (melancholic).
* The latter half of ''WesternAnimation/WheelieAndTheChopperBunch''; Chopper (choleric), Revs (melancholic), Hi-Riser (phlegmatic), and Scrambles (sanguine).
* The main group from ''WesternAnimation/{{WITCH}}'' fits: Will (Phlegmatic), Irma, (Sanguine), Taranee (Melancholic), Cornelia (Choleric), and Hay Lin (Leukine)
** Some of the guys on the show show fit too: Caleb (Choleric), Matt (Phlegmatic), and Blunk (Sanguine)
* ''Franchise/WinnieThePooh'': Pooh (phlegmatic), Tigger (sanguine), Rabbit (choleric), Eeyore (melancholic, [[TheEeyore of course]]), and Piglet (leukine).
* ''WesternAnimation/XiaolinShowdown'': Raimundo (sanguine-wind), Omi (phlegmatic-water), Clay (melancholic-earth), Kimiko (choleric-fire).
* ''WesternAnimation/YoungJustice'': Kid Flash (sanguine), Speedy (choleric), Robin (melancholic), and Aqualad (phlegmatic). After Speedy leaves, Superboy fills in for the choleric.
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