Disney's Aladdin has a famous example of unintentional innuendo in the song "A Whole New World."
I can open your eyes, Take you wonder by wonder; Over, sideways and under On a magic carpet ride. A whole new world! A new, fantastic point of view. No one to tell us no, or where to go, Or say we're only dreaming. ... Unbelievable sights! Indescribable feeling! ... A whole new world! Don't you dare close your eyes. A hundred thousand things to see. Hold your breath — it gets better!
Well at least the song has a happy ending. At the end, they hold hands as literal fireworks are shown going off…
The parody song A Whole Nude World removes the 'Accidental' part, needless to say it's NSFW..
Brother Bear: "Well gee, eh, you're one big beaver." Doubles as Fridge Brilliance if you remember that there was a giant species of beaver that existed during the time the movies take place.
Coraline: "Mothers don't eat daughters, do they?" (Only in Fanfiction and Rule 34, we hope.)
Felix the Cat: The Movie: When Pim first shows up at Wack Lizardi's office with the bag, Wack asks "What in Zill have you got to be excited about? Did someone invent enlarging pills?" (It's meant to be a crack at Pim's height.)
Frozen: In the Arabic version of "Let it Go", the part where Elsa originally sang, "Let it go/Let it go/That perfect girl is gone" translates to, "It's not coming back/It's not coming back/I've lost my innocence," with the masculine version of "it".
Let It Go, it has been alleged by certain people, can be interpreted as a song about a woman coming out.
Bakshi's The Lord of the Rings features a decidedly worrying depiction of the bit in the book where Merry and Pippin try to persuade the orcs to untie them by impersonating Gollum to trick the orcs into thinking they have the ring. Their facial expressions really do not help.
"You won't get it that way. It isn't easily found."
"It's no good groping in the dark. Uglúk will be back soon. Nothing for Mordor, nothing for poor Grishnakh. Gollum. Untie us, my precious."
Ratatouille gives us Anton Ego's line "If I don't love it, I don't swallow!"note He's an ultra-elitist food critic, and he refuses to swallow the food that he tastes if he doesn't find it absolutely perfect (the full line is, "I don't like food, I love it. If I don't love it, I don't swallow!"
When the dwarfs meet Snow White for the first time, Grumpy tells Doc to "Ask her who she is and what she's a-doin' here!" Of course, given his tendency to get words mixed up, Doc comes out with "What are you and who are you doin'?" Surprisingly enough, given the time period Snow White and the Seven Dwarfs was made in, this is probably one of the earliest examples of this trope.
In the DreamWorks move Kung Fu Panda, after Po serves noodles to the Furious Five for the first time, the group tuck in and find his cooking delicious. Mantis says "I wish my mouth was bigger!" and Monkey adds "Tigress, you've got to try this!"
Video Brinquedo: The Little Bees keep going on about their stingers, and one of the Little Cars says that the Champion gets her motor running.
Films - Live Action
Batman & Robin: Any time Bruce Wayne calls his sidekick "Dick", but the crowning example is probably "She's trying to kill you, Dick." Given how Clooney delivers the line, it might not be that accidental.
This scene in Commando when John (Played by Ahnold) convinces the villain (who honestly looks like Freddy Mercury if he was a member of the village people) to let go of his daughter.
John: "You don't want to pull the trigger. You want to put the knife in me and look me in the eye and see what's going on in there when you turn it. That what you want to do, right? Don't deprive yourself of some pleasure. Come on, Bennett, let's party."
Bennett: "I don't need the girl!"
It's also worth noting that Bennett has a very orgasmic expression◊ on his face at the thought of sticking his knife in John.
Cowboys and Aliens: An alien is killed when molten gold is dripped onto him. In other words, he gets a golden shower.
Down Periscope: Dodge does this twice when introducing Lt. Lake to the rest of the crew, much to his embarrassment.
Dodge: All right, look, gentlemen! I know this is an unusual situation. Can't be easy for Lt. Lake to be thrown into a jungle such as this, and I know it will make things hard on all of us... *crew laughs* Let me re-phrase that. It's going to make things difficult on all of us as well. But, if we just work together as a team, I'm sure we can handle ourselves... *crew laughs* ...comport ourselves as professionals. That is all.
The Toys Room: "It's not really Pamper's fault that the little boy... takes him to bed with him. I'll just have to get used to the fact that he loves somebody else besides me."
The Eagle is a film about a Roman soldier and his male slave, with maybe five minutes of screen time where a woman is actually in the movie, and no female love interest for either of the main characters, so Ho Yay seems a given. But special mention goes to the line, "Put your weight on him, slave!"
Peter: You know, I remember you being a lot bigger.
Hook: You were a ten year old, I was huge.
In the original B&W Invasion of the Body Snatchers (1956), hero and heroine are fleeing the alien pod people, and stumbling around from exhaustion (because the aliens get you while you sleep). At one point they're lying side by side, hiding in a ditch from their pursuers, and the woman blearily — and hilariously — stammers out "We'll never make it without sleep...", her lips and body suspiciously close to the man's.
Right as Chrissie is being eaten by the shark in the first scene in Jaws, the guy she took with her to the deserted beach is almost passed out drunk on the sand, breathing heavily and whispering, "I'm coming...I'm coming..." Of course, given that he's wasted and he's chasing a girl, it may be deliberate.
In Man of Steel when Dr Hamilton tries to get the Phantom Drive to work, Lois points out the S-key and yells "it's supposed to go in all the way!" Cue the audiences yelling "That's what she said!".
In Mars Attacks! Martian "paper" looks suspiciously like condom wrappers.
In Mr. Magorium's Wonder Emporium Eric takes Henry into his room to show him his hat collection. Everything was innocent, of course, but you have to wonder why Eric's mother didn't act a bit more alarmed when she found her son with a grown man in his room.
Childlike Empress: We must not give into the Nasty!
The Nutcracker 3 D: Close your eyes and the Rat King’s torture of NC sounds like the most wrong thing ever. “Open your mouth…like a good little prince!” Ditto for Frau Eva saying to Mary: “Get changed, something nice…I’ll help you.”
On Golden Pond: Upon seeing how happy Billy makes Norman, Ethel exclaims, "I should have rented him a 13-year-old boy years ago!"
The Sylvester Stallone film Oscar gets two good in-film moments from linguist Dr. Poole (Tim Curry), both involving linguistic terms — the first comes when he comments on Lisa's (Marisa Tomei's) "nicely rounded diphthongs", only to have have Snaps (Stallone's character) reply that they're "what got her in this jam [Lisa's fake pregnancy]." He later comments on another (male) character's "dangling participle", and the character responds by turning around and checking his fly. Then again, this is TimCurry (and might explain why he liked the role so much)...
The 2010 Yogi Bear movie got unexpected press at gay entertainment website After Elton.com just for the unintentionally hilarious tagline "Great things come in bears." Bears meaning somethingdifferent in the gay community... This poster◊ really did not help.
Viewed a certain way, Young Man's Fancy can be an effective premise for a porn film, when considering the dialogue.