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AH.com: The Series has Luakel, who in his various incarnations has been shot, stabbed, eaten by cannibal supermodels, turned into a bizarre squid/yeti hybrid, had his brain transferred to several different bodies, lost his virginity to a transgendered gay Welshman, etc...
Anni has to pay to work for Coyle Commander, and gets regularly abused by him. He doesn't seem to notice.
Dan O'Brien is definitely this in Cracked's "After Hours" series of videos.
Zack in Echo Chamber. Pretty much everything he says earns an eye roll from Dana and Tom.
Tom, too. For example, Mr.Administrator gets pissed off at him for something Dana said.
The Irate Gamer is a frequent victim of this trope in The Frollo Show, where he screws things up on a constant basis (likely to fulfill the Take That nature to his show). After becoming bros with Best Hercules—a villain that's more incompetent than him—they become the laughingstock of all the villains in the show.
The Downfall Hitler (of Hitler Rants fame, see below) also gets this treatment whenever the Irate Gamer or Best Hercules aren't around. Such abuse to him includes being covered with shit, sliced to pieces, and forced to wear a Sailor Moon costume in front of a crowd (causing him to die of humiliation).
The Patriot, a super-athlete who calls himself "The Ultimate New England Patriots Fan", fills this role in the Global Guardians PBEM Univerrse. Mainly because Jack Butler, who despises the patriots and all they stand for, finds it funny.
LoadingReadyRun (or, more specifically, commodoreHUSTLE) has Matt, who has a ton of bad stuff happen to him. He breaks his iPhone on the road though it gets fixed very quickly, his Xbox 360 suffers a Red Ring of Death (his ninth or tenth one yet) and is later turned into a NES Box, which is basically a NES that looks like an Xbox 360, loses a bet to Graham that allows Graham to kick him in the ballsat some point in the future, gets tricked out of cookies by Paul, travels all the way to Seattle to replace his Xbox and breaks it within five minutes after returning home (before receiving the Groin Attack from Graham), gets his idea of putting LRR videos on Youtube quickly shot down, kisses James in a dream, and lastly gets his gamer score deleted, right before the end credits. And that's just season one. Of course, the fact that he's a Deadpan Snarker, Anti-Hero, and is usually at odds with the entirety of the crew (in-universe, of course) doesn't get him much sympathy from the viewers.
You'd think that Clel from the Mario Party TV group would be this, since he loses the most, but his calm demeanor dulls the trope a little. Steeler and Holmes take turns at this instead.
Dr. Poque from Mega64 can never get his way. The worst time was when he gets into a bet to get a girlfriend with stolen money of his being used. He eventually gets one but she's taken away by one of his roommates (who's a puppet), gets made fun of by her and when it appears he won her back she's hit and killed by a car.
Mewtwo of Melee's End is mindless abused and demeaned by his boss and coworkers, even when he's not around, despite probably being the most competent villain.
In Noob, if a gag involves someone getting hurt or humiliated among the protagonists, the someone will be Omega Zell 90% of the time.
Almost every character in the dark-comedy short story Vatsy And Bruno falls under this description. Vatsy is an insane, unscrupulous journalist who can't understand why his laughably poor articles aren't making it big-time. Bruno, his stoic bodyguard, gets pretty badly thrashed in a fight against a bounty-hunter pastiche known only as trenchcoat. Most badly, the character referred to as Chinbeard gets hunted like an animal, mutated into a freakish abomination, and then put down like a dog by Bruno because it's more convenient than finding a way to turn him back.
The more annoying socks on The War Comms are this trope; Rod and Trisha are two prime examples of why this trope is considered funny.
Phase, in the Whateley Universe. Turned into a mutant, when he was one of the people who couldn't manifest as a mutant. Thrown out of his family and impoverished, tortured by a mad scientist, had to go live in a basement, his body turned into a weird inter-sexed mess so he looks mostly female, in his first superpowered scrap had his clothes burned off in public and got kicked in the family jewels, and then got chewed out by the police for stopping a cop-killer supervillain, is roundly hated by most of Whateley Academy because of his last name and his intersexed status and has been physically attacked maybe two dozen times for these reasons, usually gets injured the most in any Team Kimba battle, in their first trip to Boston he got dumped in the sewer, you name it.
In Youtube series Why Would You Eat That?, in which disgusting or odd foods are featured and fed to co-workers, the presenters typically take a special kind of sadistic delight in subjecting poor Georgia to their food of the week. It doesn't help that she reacts very negatively each time they approach, which has both goaded them on and made her a favourite among fans.
We Are Our Avatars: Donna's inability to die note he uses a cloning machine that brings him back after an hour lends to this, and he knows it.