AH.com: The Series has Luakel, who in his various incarnations has been shot, stabbed, eaten by cannibal supermodels, turned into a bizarre squid/yeti hybrid, had his brain transferred to several different bodies, lost his virginity to a transgendered gay Welshman, etc...
The Courier from Courier's Mind: Rise of New Vegas falls victim to Gunshots, broken limbs, laser burns and Cazador stings in his travels... all in the first two days of his journey.
Anni from Coyle Command has to pay to work for Coyle Commander, and gets regularly abused by him. He doesn't seem to notice.
The Yellow Guy gets all of his suggestions in the first episode slammed or ruined, including his clown picture and his favorite colour.
In the sequel, Tony beeps loudly at him until his ears bleed - something that does not happen to his friends because they cover their ears.
This continues in the third video, but not to the same extremes. Yellow Guy gets told that he can't love what he wants and must get married to a wife, against his will.
Yet again, Yellow Guy is the butt of the joke. In the fourth video he is interrupted by Duck Guy and smacked in the face twice by Colin.
Takes a disturbing turn in #5, when the Food Band tricks him into eating his only remaining friend.
It gets even worse with #6 which starts has him being dragged into a song about dreams against his will then he endures the horrors of the teachers both old and new getting swapped repeatedly when Red Guy messes with the big machine's controls and all he can do is cry and beg for it to stop.
The Irate Gamer is a frequent victim of this trope in The Frollo Show, where he screws things up on a constant basis (likely to fulfill the Take That! nature to his show). After becoming bros with Best Hercules—a villain that's more incompetent than him—they become the laughingstock of all the villains in the show.
The Downfall Hitler (of Hitler Rants fame) also gets this treatment whenever the Irate Gamer or Best Hercules aren't around. Such abuse to him includes being covered with shit, sliced to pieces, and forced to wear a Sailor Moon costume in front of a crowd (causing him to die of humiliation).
Adolf Hitler in Hitler Rants is this very much. In many videos, he receives bad news regarding one of the subjects he is interested in causing him to burst out in one of his eponymous rants. And if he is not ranting about anything, he has to deal with the cartoonish antics of his worst enemy Hermann Fegelein, among them are exploding muffins, teleporting Hitler's bunker to random locations and turning Hitler into a toilet. If Hitler tries to strike back against Fegelein, it always results in a catastrophe.
Emperor's caretaker in If the Emperor Had a Text-to-Speech Device. For starters, he has no name, and his nickname is Little Kitten. The Emperor calls him a moron half of the time, he got dumped by his first and only love and his fellow Custodians keep on mocking him, even though he's their Captain-General. Oh, and nobody knew who he actually is until Fyodor invaded the palace.
Might be subverted slightly, as Magnus notices that the Emperor trusts Kitten more than he ever trusted any of his own children. It's also notable that the Emperor keeps on insulting everyone, so Kitten is no exception.
I Hate Everything has Aqua Man and Kite-Man constantly taking verbal or physical abuse from him.
LoadingReadyRun (or, more specifically, commodoreHUSTLE) has Matt, who has a ton of bad stuff happen to him. He breaks his iPhone on the road though it gets fixed very quickly, his Xbox 360 suffers a Red Ring of Death (his ninth or tenth one yet) and is later turned into a NES Box, which is basically a NES that looks like an Xbox 360, loses a bet to Graham that allows Graham to kick him in the ballsat some point in the future, gets tricked out of cookies by Paul, travels all the way to Seattle to replace his Xbox and breaks it within five minutes after returning home (before receiving the Groin Attack from Graham), gets his idea of putting LRR videos on Youtube quickly shot down, kisses James in a dream, and lastly gets his gamer score deleted, right before the end credits. And that's just season one. Of course, the fact that he's a Deadpan Snarker, Anti-Hero, and is usually at odds with the entirety of the crew (in-universe, of course) doesn't get him much sympathy from the viewers.
Dr. Poque from Mega64 can never get his way. The worst time was when he gets into a bet to get a girlfriend with stolen money of his being used. He eventually gets one but she's taken away by one of his roommates (who's a puppet), gets made fun of by her and when it appears he won her back she's hit and killed by a car.
Mewtwo of Melee's End is mindless abused and demeaned by his boss and coworkers, even when he's not around, despite probably being the most competent villain.
The editors have gone out of there way to give Luvdisc an analysis for each generation it has appeared in, just to point out how little use it has. The Generation VI analysis can't even give any battle strategies and instead advises how it can be used to declare love to your opponent.
"Luvdisc is great if you're playing with Battle Timeout, because its mere presence should cause your opponent to laugh at it for so long that you win the match."
Downplayed with Magikarp, the trope namer for Magikarp Power and a nearly defenseless Pokemon, who only has an entry for Generation V because, as the entry sees, any jokes about using Magikarp are tired. Magikarp is described as being Nerfed because, with the new unpopularity of Death Or Glory Attacks, he lost his role as a sacrifice.
A whole episode of Plumbing the Death Star is dedicated to "Why is Hawkeye in the Avengers?", and he's frequently brought up as the worst of the Avengers. They make a point of killing him off in their What If? "Would the Avengers be Better off Without the Hulk?", they characterize him as an emotionally-fragile whiner in "Which Avengers Would Make the Best Friend?"
"Chances are you think you're better than Hawkeye thinks he is."
The poor girl in the short film ''Socket'', an exhausted electric person desperately looking for a place to literally charge her batteries.
Chef Pee Pee is definitely this as well, with Bowser and Junior bothering him all the time, and the plot destined to make him as miserable as possible. One notable example would be falling into a coma after Bowser tries riding him like a Yoshi in Part 3 of the Mario and Bowser's Stupid and Crazy Adventure story arc.
In the earlier episodes, Toad was constantly treated as garbage by the characters. This was nullified starting from "Toad is Cool".
Bowser Junior has a small status of Butt Monkiness, which escalated starting from "Bowser Junior Goes to Military School!", where he gets constantly traumatized from doing various pushups, pushing heavy rocks, passing logs, climbing ropes and walls, getting hot sauce in his eyes, scrubbing a dirty toilet with a toothbrush, watching Big Hero 6, eating dirt and watching his Thomas the Tank Engine toy being burnt before eventually getting broken both physically and mentally.
Top Down Perspective: For a while after they got bored of the game, the hosts loved dumping on the way the characters in Tomodachi Life act, mocking some of their phrases incessantly (especially "I've been working on my funny face!").
Almost every character in the dark-comedy short story Vatsy And Bruno falls under this description. Vatsy is an insane, unscrupulous journalist who can't understand why his laughably poor articles aren't making it big-time. Bruno, his stoic bodyguard, gets pretty badly thrashed in a fight against a bounty-hunter pastiche known only as trenchcoat. Most badly, the character referred to as Chinbeard gets hunted like an animal, mutated into a freakish abomination, and then put down like a dog by Bruno because it's more convenient than finding a way to turn him back.
The more annoying socks on The War Comms are this trope; Rod and Trisha are two prime examples of why this trope is considered funny.
Phase, in the Whateley Universe. Turned into a mutant, when he was one of the people who couldn't manifest as a mutant. Thrown out of his family and impoverished, tortured by a mad scientist, had to go live in a basement, his body turned into a weird inter-sexed mess so he looks mostly female, in his first superpowered scrap had his clothes burned off in public and got kicked in the family jewels, and then got chewed out by the police for stopping a cop-killer supervillain, is roundly hated by most of Whateley Academy because of his last name and his intersexed status and has been physically attacked maybe two dozen times for these reasons, usually gets injured the most in any Team Kimba battle, in their first trip to Boston he got dumped in the sewer, you name it.
In Youtube series Why Would You Eat That?, in which disgusting or odd foods are featured and fed to co-workers, the presenters typically take a special kind of sadistic delight in subjecting poor Georgia to their food of the week. It doesn't help that she reacts very negatively each time they approach, which has both goaded them on and made her a favourite among fans.
In most RedLetterMedia productions, particularly Best of the Worst, Rich Evans is often treated like this - he accidentally slices his finger off with a saw at the end of one episode and gets repeatedly punched in the face by Len Kabasinski.