- Base-Breaking Character: Dudley. Some find him funny and endearing thanks to him being voiced by Jerry Trainor of iCarly fame, others find him obnoxious and intolerable.
- Broken Base: Depending on who you ask, it's either a really funny show and one of the better Nicktoons from Nick's Dork Age, or Butch Hartman's worst show and another example of Nick's Dork Age.
- Draco in Leather Pants: Madame Catastrophe gets this very often in the fandom. Dr. Rabies does not, generally being turned into pure evil.
- Ensemble Dark Horse: Madame Catastrophe and Dr. Rabies.
- Little Chipmunk Girl
- Tammy the secretary.
- Daisy, Dudley's girlfriend from "Girlfriend or Foe".
- The Fiendish League of Potential Perpetrators (F.L.O.P.P.)
- Fan-Preferred Couple: Dudley/Kitty.
- Ham and Cheese: Even those who hate the show can't help but laugh while Snaptrap chews the scenery.
- Ho Yay: Dudley and Keswick even share a "Lady and the Tramp" kiss in "Law and Odor".
- There's also the time that Bird Brain shot Dudley with a love ray in "Love Bird".
- I Am Not Shazam: Dudley is only referred to as T.U.F.F. Puppy in nickname form by Kitty and that's only once and it was said sarcastically.
- Moral Event Horizon: Jack Rabbit betraying Kitty and T.U.F.F.
- Older Than They Think: A wolf popstar that Kitty's a fan of is named Justin Timberwolf, as mentioned in "Booby Trap." The Simpsons had already named Maggie's wolf plushy that in the episode "Midnight Towboy."
- One-Episode Wonder: Jack Rabbit, Dr. Rabies and Madame Catastrophe, Snowflake and Slush, Daisy, and Katty, to name a few.
- Periphery Demographic: Among the Furry Fandom.
- Strawman Has a Point: Kitty was pretty much against working with Dudley and tends to still be from time to time. Given what Dudley does from time to time, it isn't unreasonable.
- Suspiciously Similar Song:
- Compare this show's theme song with that of Teen Titans and tell me there isn't a similarity.
- Given the spy theme of the show, the song sounds vaguely similar to Secret Agent Man.
- Tough Act to Follow: Being Hartman's first new project since Danny Phantom. Unfortunately, it never could reach that show's level of reception by viewers.
- What an Idiot!: Has its own page.
- The Woobie: Kitty Katswell. Her status as The Chew Toy at T.U.F.F. goes hand in hand with this. Her Amusing Injuries are usually funny, sometimes though: oh poor Kitty.
- Dudley has his moments too, even if you consider him a gigantic nuisance at times.
- Jerkass Woobie: The Chameleon pretty much lives and breathes this trope. It's played for laughs, as he's supposedly nasty thanks to being depressed about being alone, but half the reason he's alone is because he's such a nasty guy in the first place. It's not played for laughs when he almost gets T.U.F.F and D.O.O.M to destroy each other and the entire city by tricking them into destroying the city/their "bases," sharing an office, and watching as a real estate agent.
YMMV / T.U.F.F. Puppy