Boss Rush: Upon beating the game, you can get this as an upgrade at the Spidey Store. Go back to the warehouse where you first fought Shocker, and you get to play rounds against bosses and their mooks. Mysterio is not included and instead replaced by Calypso, though bowlhead's mooks remain. Each round escalates the opponents, including four bosses at once.
Bowdlerize: Road rage dialogue in the game is vastly G-rated compared to real life.
Celebrity Voice Actor: The game has the voices of Tobey Maguire (Spider-Man), Kirsten Dunst (Mary Jane), Alfred Molina (Dr. Octopus), and Bruce Campbell as the Narrator. Additionally, whilst some movie characters are not voiced by their film counterparts, they are played by notable character actors such as Mindy Sterling (Aunt May) and Susan Egan (Rosalie Octavius).
Continuity Nod: You can find The Vulture's feathers in the area where you fought him in the previous game.
The Dev Team Thinks of Everything: Everything in this game, and in a semi-Tear Jerker moment, you can see the 9/11 memorial at night. Also, one of the first missions in the game involves Peter being late for one of Dr. Connors' lessons. The game doesn't time you on this one like the others, however. If the player simply jumps from building to building without web swinging, the narrator calls them out on it. If the player continues, the narrator does it again.
Dummied Out: Governor's Island was also supposed to be included as a bonus accessible area along with the Ellis and Liberty islands. Time constraints forced the dev team to remove it, but its title card remained in the game.
Exact Words: The narrator says that, after you read all the helper tokens, they will say something different, and says you should try to find them all. This is literal. They all say "something different".
Gotta Catch 'Em All: You can find skyscraper tokens, hideout tokens, "secret" tokens, challenge tokens, buoy tokens, and 216 "helper" tokens.
Hall of Mirrors: Part of Mysterio's base. Wobbly "clones" of Spidey will emerge from the mirrors and attack.
Mysterio's alien fakeout. The second Amazing issue featured a back-up story where Spidey fought the Tinkerer and Little Green Men. Years later, Roger Stern wrote a retcon revealing these "aliens" as men in disguise committing crimes for the Tinkerer and one of them was Quentin Beck.
Prank Call: One of the "ambush" citizens says, "Is your refrigerator running? 'Cause I sure am!" Unimpressed, Spidey replies, "No, but I have Prince Albert in a can..."note "Prince Albert" is a brand of tinned chewing tobacco; the punchline is, "Well, you'd better let him out before he suffocates!"
10-Minute Retirement: In an inverse of the film's plot, Peter decides to give up his civilian life and be Spider-Man 24/7 only to immediately change his mind.
Video Game Cruelty Potential: Sure, you can beat up thugs, but you can also throw them against a wall, into water or off a building. After you have beaten them and gotten the points, you can continue to beat on them just for the fun of it. This can also be done to Rhino and Mysterio, the former of which will beg you to stop.