The entire train sequence. And just about anything involving Doctor Octopus.
Doctor Octopus gets his own crowning moment by responding to the civilians. Long story short, the civilians say "If you want to get to him, you'll have to go through me." Ock simply replies "Very well" and flashes a big ol' grin. Then he simply holds them out of the way with his arms.
It's not Spidey saving an entire trainload of people from horrible death (who then save him). It's not the civilians (later) standing up to Doc Ock (though both are awesome). Then Peter realizes his mask got lost and has a little moment of panic and the two kids say 'We won't tell nobody'. The others agree.
Made more awesome by that one guys shocked reaction, realising the sort of person who routinely risks his life to save them.
Passenger: He's... just a kid! No older than my son!
"Shame on you." You just know Doc Ock's in for it when Aunt May shows disgust towards underhanded sneak attacks...in more ways than one. The film version of Aunt May's Moment of Awesome was smacking Doc Ock upside the head with her umbrella handle, temporarily distracting him while both are at least two dozen stories high (300 feet up at least). She had the theater crowd cheering.