Ash from Evil Dead appears again, this time keeping Spider-Man from seeing Mary Jane's play.
Joel McHale as the bank teller, and Donnell Rawlings (Ashy Larry) as the witness of the pizza "heist" (the source of this film's Memetic Mutation, by the way). Apparently, Dave Chappelle himself was supposed to be the guy in the elevator, but for whatever reason he was replaced by Hal Sparks of Queer as Folk/Best Week Ever fame.
Joel would reference his cameo three years later in an episode of The Soup with a spoof trailer of Spider-Man 3, where his bank-teller character becomes a Magnificent Bastard.
Michael Beattie reprises his role from the first game as the Shocker.
What Could Have Been: The 2003 trailer sported a more cel-shaded style, and showcased the Lizard, who never made it into the game (though there were reptile men of sorts in the game, though only in an unlockable bonus mode).