- Accidental Innuendo: This exchange between Peter and Mary Jane:
Peter: Picking up where we left off.
Mary Jane: Where was that? We never got on. You can't get off if you don't get on, Peter.
- Better on DVD: Spider-Man 2 was improved for Spider-Man 2.1 with extended battles and improved character development which makes this an Even Better Expansion of an Even Better Sequel.
- Ensemble Darkhorse:
- Ursula, the shy daughter of Peter's landlord, who a substantial amount of fans would have preferred to see Peter get together with rather than MJ.
- Doc Ock, who is often considered the trilogy's best villain.
- Even Better Sequel: While the first movie is considered good, this is widely thought of as one of the greatest superhero movies of all time.
- Genius Bonus: The play M.J. is in is The Importance of Being Earnest, a comedy about double identities. Sound familiar?
- Growing the Beard: Fans of the original Raimi trilogy generally consider this to be the best installment.
- Harsher in Hindsight: Doc Ock's "I will not die a monster" moment at the end of the film can be a bit painful to watch given that his plan in Ends of the Earth is to cause genocide on a planetary scale and be remembered as history's greatest monster.
- Hilarious in Hindsight: Jameson briefly implies that Doctor Strange exists in the movie's 'verse. He would later join the Marvel Cinematic Universe just one film after Spider-Man did.
- Memetic Mutation:
- Whoa.....he stole that guy's PIZZA! Quickly became one of the site's most popular fads, with imaginative re-cuts of films and music videos with Donnell Rawlings, even including the two musical stings right after his line.
- J. Jonah Jameson's laughing face. Many knows him as the Aww Yea Guy.
- "Pizza time.", which has essentially become a Catch-Phrase for Spider-Man in the many Youtube Poop parodies of the films.
- Mr. Aziz yelling, "GOOOOOOOOOooooooooo!!!" However, in 2.1, he just said "GO!".
- The many facial expressions of agony Peter makes while trying to stop the train, which are quite hilarious when viewed out of context.
- The particularly over-the-top scream that Peter does when he sees a wall falling towards MJ has been a popular source for remixes.
- Most Wonderful Sound: Jameson's laugh.
- Narm: The actors who were cast to play the extras for this movie hardly ever felt like they were natural human beings. The worst offender was when Doc Ock opens up the train car to take an exhausted Peter with him, and everyone who stood in his way gave a halfhearted "you're going to have to go through me" one-liner.
- Also, Doc Ock's tentacles having a built-in AI that speaks to him (words the audience cannot hear) would have been a truly creepy addition...had the film not have the tip of the tentacles open and close like mouths in a puppet-like fashion whenever they're speaking to him, which just looks goofy.
- Signature Scene: The train scene is the first thing that pops up when you remember about this movie. As an added bonus, scientists who have been working on replicating spider silks as a new clothing material like to refer to this particular scene as their source of inspiration.
- Suspiciously Similar Song: The music used in Otto Octavius' ill-fated test run of the fusion reactor is virtually identical to the main theme from Hellbound: Hellraiser II.
- Tough Act to Follow: No subsequently released Spider-Man film managed to reach the movie's critical acclaim until Spider-Man: Homecoming came out 13 years later. The game seems to be this for every subsquent Spider-Man game, especially when it comes to the web-slinging mechanics of later games.
- What an Idiot: Really, Peter? You could test your powers anywhere safe by trying to climb a wall, but you go and jump of a building? Firstly, the fall could have been lethal, and secondly, what if somebody who knew May had seen it? Who knows what might have happened if somebody told May that Peter jumped from a roof in an apparent suicide attempt.
- Anticlimax Boss: After he pulls off a convincing alien invasion hoax and a trippy "funhouse of doom", you'd expect Mysterio to have an imaginative boss fight, especially when the cutscene prior gives him three health bars. Instead, he just stands and boasts while doing nothing to harm you, and one punch does him in.
- Big-Lipped Alligator Moment: The entirety of the Mysterio plotline is this, but perhaps it's at its biggest at the "Funhouse of Doom" section. Spider-Man goes through a bizarre funhouse with wobbly clowns, creepy holographic heads and a hall of mirrors where his reflections come to life and try and kill him... and it's all seemingly within an ordinary apartment building. And all this is never brought up again.
- Demonic Spiders: The mooks using Powered Armor. They're strong, durable, and have weapons that can knock Spidey down for a few seconds. Even worse is that after you first fight them in the plot, they can show up when taking a civilian mission.
- Even Better Sequel: Compared to the previous movie adaptation game, Spider-Man 2 is an improvement on every level, being regarded as one of (if not the) best superhero games.
- Memetic Mutation: "Ooohh noooo... Doooctooor Cooonnerrrss' claaassss..."
- No Problem with Licensed Games: The video game adaption is also held in high regard as one of the best superhero games of all time (many considered it THE best until the release of the Batman: Arkham Series five years later), and alongside The Incredible Hulk: Ultimate Destruction and the X-Men Legends games, revived the "Superhero Sandbox" genre after Superman 64 destroyed its credibility.
- Most Annoying Sound: "Oh no, my balloon!"
- The Problem with Licensed Games: While the console versions are very well-regarded, the PC version is completely different, being much simpler and mediocre, having a totally different plot, and lacking the size and complexity that made the console versions so beloved.
- Scrappy Mechanic: People hate the kids who lose their balloons. The Ultimate Spider-Man game took a jab at it by having the first person that the player-controlled Venom kills in the game be an obnoxious little kid with a balloon. In the same vein, the children's crying and whining over their lost balloons for almost every player became the Most Annoying Sound.
- That One Level: "When Aliens Attack" can be rather annoying; at one point, you must web swing to the Statue of Liberty on UFOs, which can be rather finnicky due to their small size, and then you have to destroy eight orbs on the sides of a larger UFO. It's very easy to fall down, and maintaining the right altitude to reach the orbs is difficult. Mysterio's "Funhouse of Doom" in the same chapter is also difficult, and is a borderline Marathon Level thanks to the final area requiring you to break several mirrors and defeat durable clones that spawn from each one.
- Uncanny Valley: While Mysterio's helmet is usually obscuring, you can see what's underneath via two holograms in the Funhouse of Doom. It's... not exactly a pleasant sight.