She eventually succeeds in the final episode. She tosses her flowers in shock and Basara manages to catch them, on the fly no less.
Freya from Princess Tutu is a beautiful, kind girl that can hear the "voices of the flowers". When Mytho briefly casts a spell on her to convince her that she is better than everyone and should give her heart to him, dark, raven-shaped flowers cover her flower bed and cut off the voices of her beloved plants. Tutu is able to break the spell when she points out to Freya how the voices are missing.
Naruto subverts this, since Ino Yamanaka works in her family's flower shop and has great knowledge of flower language... but she's a strongwilled, somewhat vain Tsundere.
Sora Takenouchi also subverts this, though she does eventually get into her mom's business by the time 02 rolls around. Coincidentially, she and Ino are both voiced by Colleen O'Shaughnessey.
Tsubomi Hanasaki a.k.a Cure Blossom, from Heartcatch Pretty Cure, loves flowers, lives in a flower shop with her family, and aspires to be a botanist. She is also so shy that flowers and plants were her only friends until she met her Pretty Cure partner Erika. Her knowledge about flower is so deep that she's able to point out the meaning of every flower residing in the hearts of former victims of the Desertrians.
Michelle Ratockie from Mobile Suit Gundam sells pastries, but otherwise she fits in perfectly.
In a filler episode of Detective Conan we meet a young woman named Midori Nozaki, the sister of a famous ikebana arrangement expert. After her rival Rika Ookano and her sponsor Shiraki steal her secret to make flowers bloom eternally and get her blacklisted from the ikebana circles, the eldest Nozaki kills herself with poison, and Midori starts carrying her revenge by working with the culprits and gaining their trust so she can kill them when they lower their guard. She manages to kill Shiraki by drugging and then strangling him, but Conan stops her when she's about to exact the revenge on Rika andkill herselfon stage at the same time, using the same techniques that the deceased Nozaki-san used in her suicide.
Oz meets one in Pandora Hearts. Her horrible death was the demonstration of what happens when an illegal contractor's seal is completed.
Horribly subverted in the first Fatal Fury OVA. We see a cute and shy girl named Lily with a basket of flowers, and she approaches a man named Jeff Bogard to ask him if he can to buy some of her stuff - so far, so good. But the kinda cute scene turns into tragedy when Jeff is attacked by several men in black, knifed as he tries to shield Lily... and then murdered by the mooks's leader Geese Howard. It turns out that Geese forced Lily to act as a Decoy Damsel so he could have a chance to kill Jeff. Flash forward to 10 years later, and when Jeff's son Terry comes back home to face his past and have Revenge, he meets the adult Lily as a beautiful Femme Fatale who has never forgotten her terrible stunt as a flower girl...
Eileen from Yami No Matsuei. Horrifyingly enough, she was kidnapped and murdered by Muraki so her heart would be given to Tsubaki Kakyouin, Eileen's closest friend, years before the story even started. When Tsubaki found out from Muraki himself, she felt so horribly guilty that with some more push from him, she developed a Split Personality named after Eileen, which started killing people in revenge.
Martha from Honoo No Alpen Rose, though she's more outspoken than the standard. She's the one who offers Lundi some alpine roses and sings the Alpine Rose song, which tips Jeudi and Lundi off in Jeudi's Mysterious Past.
A traveling show in The Twelve Kingdoms includes a small girl who makes little rabbit heads on sticks and sells them. She's the first characters in that world to break through the protagonist's growing cloud of despair.
In Tatsuya Shinjyouji's The King of Fighters 94 manga, Terry Bogard has quite a bit of Ship Tease with an Italian flower girl named Marucco, who also has ties to an Orphanage of Love. Unlike Lily and Sulia from the Fatal Fury animes, Marucco doesn't kick it — though in the second volume she and her friends are targetted by some mafiosi, but Terry helps them.
The original "The Little Mermaid", the tragic one, grew underwater flowers and plants in her spare time. One VHS cover of the movie has Ariel sitting on a rock preoccupied with underwater plants, possibly as a Mythology Gag.
Kes in Star Trek: Voyager built her own hydroponics garden in Voyager's cargo hold, though it got burned up when she suffered one of her rare Creepy Child moments. Most of the time however Kes is portrayed as kind, innocent and understanding. She also suffered a tragic fate, being booted off the cast for a busty blonde in skintight catsuit, only to return a few seasons later as an Axe Crazy granny in a revenge plot with an ill-conceived motive and an unconvincing resolution.
Possibly Kendall, a florist that Black Ranger Danny pines after in Power Rangers Wild Force. Of course, since Danny is her coworker in the flower shop and a Gentle Giant, he really fits as an Innocent Flower Boy.
In the Dracula film from 1931, the titular character preys on an innocent flower girl in London.
Let's not forget the blind flower seller in Charlie Chaplin's City Lights. At the end of the movie, thanks to the Little Tramp, she's had an operation to restore her sight.
The film Alegria, inspired by the Cirque du Soleil production of the same name, has a whole troupe of unwanted, unloved kids under the thumb of an abusive taskmaster who forces them to sell flowers on the streets, as well as tend to them in the warehouse they're imprisoned in. They are inspired to revolt at the end. Sadly, there's Truth in Television here: the director got the idea for this when he was approached by such a kid.
Thel Russel from Dead Man, a sweet and abused young woman who makes paper flowers and gets murdered at the beginning of the film.
Subverted for laughs in Terry Gilliam's Yellowbeard.
Toyed with in Hayao Miyazaki's adaptation of Howl's Moving Castle. Sophie ends up owning a flower shop, and while she becomes stronger in the process she also remains kind and gentle, not going through levels of jerk-ass.
Japanese Mythology has Konohanasakuya-hime, the blossom-princess and goddess of Mount Fuji. She fell in love with Ninigi no Mikoto, the grandson of Amaterasu, who liked her as well and persisted in marrying Konohana when her dad, Oho-Yama, wanted Ninigo to marry her older sister Iwa-naga. However, Ninigi soon suspected her of infidelity since Konohana became pregnant in just one night; enraged at Ninigi's accusation, Konohana trapped herself in a doorless hut and set it on fire, vowing that the child would not be hurt if it were truly the offspring of the Ninigi. Not only she gave birth to triplets (Hoderi, Hosuseri and Hoor), but neither of them was hurt so her innocence was proved and she was absolved.
Mimì from the Puccini opera La Bohème, despite being actually a seamstress, fits in well otherwise. (Absolutely not Mimi from RENT.)
Ophelia, Hamlet. Depending on one's interpretation of the scene, the flowers Ophelia hands out may only exist in her mind. And they're not so innocent, considering each of her flowers represents a thinly veiled criticism of the people she hands them to.
Averted by Pygmalion. Eliza Doolittle may be a flower girl, but she's far from innocent.
She's not exactly innocent - she just acts that way. Refusing to buy a flower nets Ark a nice little insult (though admittedly, he does refuse really tactlessly).
Florina from Wild ARMs 3. She can "feel" the pain of the planet, grows flowers, and, after a sidequest, will grow the usually hard-to-find healing berries for you. In a break from the convention, she doesn't die at the end of the story.
And Mariel from Wild ARMs; Florina is pretty much an Expy of her. In the remake, Mariel can join your party, but she can't really attack enemies, only use healing abilities based on the ubiquitous berries.
Luty from Xenosaga is a little girl who became mute from the trauma of her home planet Ariadne disappearing some time before the story begins. A sidequest involves finding and growing a flower from Ariadne, which cheers her up enough to gradually get her to speak again. When encountered again in Episode II, she begins to work as a nurse, and is involved in another sidequest, where she wants to grow a garden of Ariadne Flowers to help make everybody in Second Miltia happy.
Pokémon Red and Blue has Erika, the flower shop clerks behind the Petalburg forest, and, to an extent, the entire town of Floaroma.
A character literally named Lil' Flower Girl in the Precious Miseries doll and art series is dark example of this trope, wearing a black and pink dress decorated in spiderwebs and skulls. Her description on the tag of the doll reads as follows:
"Dressed in her best outfit, she goes forth into the streets to sell her dead flowers. Because she is not very talkative, when SHE finally decides who is to buy from her, she will follow them all day with her arm extended and flower in hand."
Mocked pretty thoroughly in Edward Gorey's The Hapless Child.