- Alternate Character Interpretation: Tip's actions in the "Tin Soldier" arc made some fans quite suspicious as to whether he planned Chris and Marcie getting together or Moustachio overheating after their Therapy Puppets session, preventing his rampage. So, was he just screwing around, or is he really a Guile Hero with textbook Obfuscating Stupidity?
- Cargo Ship: Nick/Dr. Lee is a very popular pairing, which the the authors are actually hinting at.
- Creepy Cute: Most of the basement-dwellers. The Cobras That Want Hugs stand out.
- Crosses the Line Twice Destroying small children is one of the things Anasigma does best!
- Ensemble Dark Horse: Dr. Virginia Lee, a.k.a. Tip's not-a-girlfriend, a.k.a. the smokin' hot Asian chick. Never has so much fan art of a minor supporting character been produced in so little time. Following her appearance, all of the fan art with the cast included her as a main character.
- And, as of "Tin Soldier", she seems likely to be upgraded to a recurring character.
- Genius Bonus: When being accosted by squirrels, they refer to Tip as long pig.
- Hilarious in Hindsight: Nera's "It's just like my 'My Little Pony' fanfic!" comment near the end of the "Choose" arc becomes this in light of a My Little Pony fanfic that started publishing a few months later, inspired by this arc.
- Memetic Mutation: In the comments for this comic. "HUGS!"
- Squick: According to Unity, Bubbles sometimes forgets she isn't a water cooler anymore and pees on people. Since she's a robot now it's clean at least, but still.
- Viewer Gender Confusion: Tip is a man who dresses as a woman, but he sometimes comes off as just a flat-chested woman to those who don't know better. His Gender-Blender Name doesn't help.
- The Woobie: Jonah is Blessed with Suck. Sure, the teen initially thinks his brain damage is awesome, but all those horrible deaths keep sending him back to the most boring movie ever. It's implied that even when he escapes Anasigma, there can be no real Happy Ending, because he hasn't broken from the "Groundhog Day" Loop. A variant of Who Wants to Live Forever??
YMMV / Skin Horse