The main character, a disc jockey. He is the manager of the pet center, and is best friends with Bo Monkey.
- Attention Deficit... Ooh, Shiny!: Twists attention is limited.
- Butt-Monkey: Not always, but he does have a tendency to fall down and knock things over.
- Plucky Comic Relief: A perfect example of this.
- The Protagonist: Twist definitely gets the most screen time and lines of any character, but the show does its best to focus on different characters in each episode. Twist is listed first in the credits and is almost always in the front of the group, so he is considered the protagonist.
- Took a Level in Dumbass: The live-action Twist was fun-loving and goofy, but now that hes animated, Twist is also klutzy and ditzy.
Both the lead singer and guitar player of the band. She runs a salon with the help of the other Fresh Beats.
- Flat Character: Theres not much to her personality aside to the fact that she owns a hair salon.
- Out of Focus: When the episode doesnt focus primarily on her, Kiki doesnt do much. This occasionally happens to Shout and Marina as well.
- Pink Means Feminine: She always wears pink, and most of her hair salon is decorated in pink.
- Tomboy and Girly Girl: The Girly Girl to Marina's Tomboy.
The Fresh Beat Band's keyboardist, who also works as a lifeguard.
- Catchphrase: Shoutstanding!
- Hero-Worshipper: Only for the beginning of Mummy Mayhem, when Shout obsesses over Arizona Jones, whom he has idolized since he was a child. He soon learns that Arizona is a thief, and never worships his hero again.
- Orange/Blue Contrast: He is most often paired with Marina, probably because their outfits are complementary colors.
- Token Minority: The only member of the band with a dark skin tone.
The drummer of the Fresh Beat Band. She is very intelligent, and works as the chemist for a cupcake bakery.
- Catchphrase: Brainstorm!
- Flanderization: Marinas intelligence turns her into a scientist-type character in some episodes (for example, Rocket Ship Alien).
- Tomboy and Girly Girl: The Tomboy to Kikis Girly Girl.
A fun-loving monkey and Twists best friend. He does anything and everything with Twist.
- Butt-Monkey: Bo often falls down or walks into something for comic relief. Plus, hes an actual monkey.
- Everything's Better with Monkeys: A perfect example of this, if not the best example of this.
- Species Surname: Bo is a monkey, and hes called Bo Monkey.
- Team Pet: He could be described as one.
The gadget guy of the team. He spends most of his time in the music store, which turns into the spies secret headquarters.
- Adjusting Your Glasses: Reed has adjusted his glasses by the corner.
- Flanderization: In the live-action show, Reed was a clever guitar player. In the animated show, both his personality and appearance have been changed so that hes a complete nerd.
- Nerd Glasses: Reed wears a pair of round green glasses.
- Nice Hat: He wears a fedora.
- The Other Reed: Though Reeds voice actor (Tom Kenny) stays the same for the entire series, he was not portrayed by Kenny in the original show. He had been played by Patrick Levis in the final seasons of the live-action series, and no reason for his voice actor change has been given.
- The Smart Guy
- Satellite Character: Reed seems to interact only with the spy team and Commissioner Goldstar.
- Smart People Wear Glasses: Reeds definitely intelligent, and he wears glasses.
Commissioner Goldstar is the chief of police who appears at the end of many episodes, usually to thank the Fresh Beats for saving the day. He has the authority to arrest the villains the characters encounter.
- Catchphrase: "Good work, Fresh Beats."
- Cool Old Guy: The Commissioner is the oldest of the main characters, and hes the well-known and respected chief of police.
- Cool Uncle: The Commissioner is the cool uncle to his niece Lily.
- Happy Dance: The Commissioner performs the same "happy dance" in three episodes: "Fruit Racer Game," "Fake Fresh Beats" and "Ghost of Rock."
- Meaningful Name: Commissioner Goldstar always wears a gold star on his shirt.
- The Voiceless: The Commissioner does not speak at all in the episode "Ghost of Rock." He only appears in the background of one music video scene, performing his signature dance.
Champ von Champ von Winnerchamp
A villain who loves to collect objects, usually by stealing them. He debuted in "Trophy Trouble."
- Meaningful Name: He loves to win and be a champion. Hence his name, Champ von Champ von Winnerchamp.
- Only One Name: An extreme aversion. His name is Champ von Champ von Winnerchamp, and in "Masked Wrestler," he adds a third "Champ von" to his name for no good reason.
- Recurring Villain: Von Winnerchamp is the most frequently-seen antagonist in the show.
The captain of a pirate crew who sings. Appears from time to time, usually to steal something with the help of his First Mate.