Jim MacGeorge (1962)
Mark Hildreth (1988)
The little boy in a Beany copter.
- The All-American Boy: Fits this trope very well.
- Catchphrase: HELP! CECIL, HELP!
- Eyes Always Shut: The Beany puppet used on Time For Beany had his eyes shut. Animated Beany generally has both of his eyes shut as an homage to the original puppet.
- Interspecies Friendship: He's a human boy who is friends with a sea serpent.
- Meaningful Name: He wears a beanie hat.
- Parental Abandonment: His parents are never so much as mentioned.
Bob Clampett (1962)
Billy West (1988)
The green sea serpent.
- Catchphrase: I'M COMING, BEANY BOY!
- Interspecies Friendship: He's a sea serpent who is friends with a human boy.
Uncle Captain Huff 'N Puff
He is Beany's uncle and sailor.
- Cool Uncle: He's always bringing his nephew and Cecil along for adventures.
Irv Shoemaker (1962)
Maurice LaMarche (1988)
The main villain of the series.
- Card-Carrying Villain: He’s nasty and proud of it.
- Phrase Catcher: Cecil: D.J., you dirty guy!
- Dastardly Whiplash: He dresses in black, has a pencil thin mustache, an Evil Laugh and is a rotten sort.
- Signature Laugh: NYAH-HA! HA!
An alien from the planet Saturn who speak in rebus puzzle ballons.
- The Gadfly: His entire shtick is messing with Dishonest John.
A Detective bear who helps Beany and Cecil with hunting gangster bears. His name is a play on Yogi Bear.
- One-Shot Character: He only appears in The Warring 20s.
Edgar Allen Poe Shadow
A shadow man who works at the haunted mansion museum.
- No Celebrities Were Harmed: He is based on Alfred Hitchcock.
- Elmuh Fudd Syndwome: He pronounces R's as W's. Even his voice is similar to Elmer Fudd's.
- One-Shot Character: He only appears in Beany and Cecil meets the Invisible Man.
Double Trouble Bubble Beast
An underwater creature who blows a bubble on top of his head.
- One-Shot Character: He only appears in Ain't that a Cork in a Snorkel and Makes a Sea-Serpant Sore!
So What and the Seven What-Knots
A Dixie jazz band that performed at the What-Knots cottage jazz club in Lost Wages, Nevada.
- No Celebrities Were Harmed: They are based on the fairy tale book, Snow White.
Snorky the Sea Serpent
A character in the Musical Episode, The Tale of Snorky the Sea Serpent (AKA There's No Such Thing As A Sea-Serpent).
- One-Shot Character: He only appeared in this short.