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Berry from Fosters Home For Imaginary Friends acts sweet and kind, but is homicidally jealous of anyone who she sees as a threat to her relationship with Bloo, which is even more imaginary than she is.
The eponymous character of Pucca. She's smitten over Garu, but since he thinks Girls Have Cooties, he's not having any of it. Ever. Except this one time... when Garu is told to get Pucca to her birthday party while she cries a waterfall, and he sucks it up for a couple seconds to blow her a kiss to get her spirits up.
Panini on Chowder is obsessed with the title character, to the point where it's scary at times.
"I just wanted to pass laws to make you my property!"
Wendy, Stan's love interest/girlfriend in early seasons of South Park. She went so far as to have a substitute teacher (later revealed to be lesbian) arrested on false charges and locked inside a rocket launched directly at the sun, for the crime of having been the object of a brief crush from Stan.
Azula from Avatar: The Last Airbender fits this when you think about. Her entire Villainous Breakdown is caused by Mai and Ty Lee turning on her for Zuko, who she is thereafter ready to kill on sight. One can also say she is a yandere for the position of Fire Lord in general. For when she is put into a fire nation mental asylum and is presented with the chance to aid Zuko in finding their mother, she states that he need not reward her, for serving the Fire Lord is reward enough. And all things considered...
Sadira is a "street rat" like Aladdin was, and falls for Aladdin the way he did for Jasmine, but, since he's in love with Jasmine he refuses her. Then she stumbles onto an ancient library of forgotten magic, and the end result is increasingly dangerous plots to get rid of Jasmine and take Aladdin for herself, including rewriting reality at one point and altering his mind. Eventually she gets over her crush and befriends the gang, but almost has a brief relapse when she believes they don't trust her.
Jasmine, due to Criminal Amnesiac, becomes this in "Forget Me Lots". She only returns to normalitynote and stops trying to kill Aladdin when Aladdin remembers their anniversary.
Quagmire marries one (Joan) in the episode "I Take Thee Quagmire" in Family Guy. Peter convinces him to get a divorce using champagne and Lois's breasts, and when he tries to broach the subject she threatens to cut herself with a kitchen knife and then cut him.
"And I love you too, Crazy Woman I'm Having Second Thoughts About!"
Meg is Yandere for Brian in "Barely Legal".
Meg, in general.
Stewie has also shown to be quite the Yandere towards whoever he may express an attraction to or fall in love with, male or female.
During one episode of Teen Titans, Starfire shows a few Yandere tendencies when Kitten blackmails Robin into going to her high school prom with her. At one point, she crushes the front end of a car with a single punch. She stops once the forced date plotline is resolved. Of course, there's the part where she rises out of the punch bowl like an alienJawsabomination. (2:01)
In one Looney Tunes cartoon, Daffy must exorcise a possessed heiress, though there's open room for interpretation that she was actually one of these.
Leading off, Princess Mandie (pronounced Man-DIE...which is very fitting). "Nobody leaves me at the altar! NOBODY!"
And there's "Just The Two Of Us", where Trixie goes batshit insane on Timmy because he wished that they were the only two people on Earth, and being the Attention Whore she is, can't handle not being worshiped by a huge number of boys, Timmy not being able to keep up with her demands.
Or Super Bike, an animated bike, towards Timmy.
One episode of The Oblongs had Helga Phugly become one towards Milo because she wants him to take care of her due to her parents being absent.
The Planet Express Ship. She tries to kill herself and the rest of the crew after Bender breaks up with her.
Bender himself towards Fry, albeit platonically. When Fry discovers his old dog Seymour's corpse fossilized in a museum, Bender becomes increasingly jealous until, when Fry announces he's going to clone Seymour, Bender grabs the fossil and throws it into a live volcano (in a near-literal Kick the Dog), screaming that he's never, ever going to let himself be replaced as Fry's best friend if he can help it.
The Hunchback of Notre Dame: Frollo is a strange one. It's certainly not love, for obvious reasons. It's also not just the desire for sex, because then any woman would suffice. It's more like an unhealthy obsession. Which, of course, fits the definition of Yandere to a tee. Now throw in a ridiculously rigid version of catholic sexual moral, and you get one of the greatest Disney villains of all time.
On American Dad!, Francine Smith has shown these tendencies towards her entire family, but most of the time it is directed toward her son Steve. The whole thing gets taken to an especially creepy length in the episode Iced Iced Babies.
Francine: You can stay right here. With mommy. Forever.
Also Nergal Jr as, like Nergal, his dad, wants desperately to make friends but if people refuse to be his friends, he freezes them and takes their form.
Heloise on Jimmy Two-Shoes. Among the things she's done when Jimmy hasn't noticed her is doing a "Freaky Friday" Flip on Beezy and Cerbee and fusing Jimmy and Beezy together. There's also "Best Bud Battle", where Heloise chains Jimmy up to keep him from leaving her and locks him in a tiny cage.
Whitney Stane from Iron Man: Armored Adventures. Her feelings for Tony appear to be yandere, becoming violent if there is a possible threat to Tony's life, even going so far as to try to kill her own father as she believed he was trying to harm Tony.
Breach towards the titular Generator Rex...sort of. While she does have an unhealthy obsession with him (kidnapping him to make him her boytoy, and refusing to let him go even when her life was in danger because of him) it's never been confirmed that she likes him romantically. (If we take her literally, she just thought Rex would be a great action figure.) There's enough evidence for fans to run with, though.
For a definite example, there's that Creepy Child minion of Breach's. She's never named, but she's absolutely obsessed with making Breach happy and tries to kill Rex when she realizes Breach likes Rex more.
Yo towards Chum Chum in Fanboy and Chum Chum. She's obsessed with taking him home, dressing him up in a sailor outfit, and putting him on her toy shelf. She even trapped him in a giant virtual pet case at one point. Also a Genki Girl.
Mother Gothel from Tangled is this to Rapunzel, though it's not for Rapunzel herself but for her hair which keeps Gothel young and beautiful. Towards the end, she gags Rapunzel, puts chains on her and drags her to the basement so she won't escape.
In an episode of Disney's Hercules cartoon, Hercules gets an idea when he learns about Pygmalion and tries to make his own dream girl out of clay. Unfortunately, being a hormonal teenager, all the personality he can think to give her is "in love with me". Aphrodite, who Hercules has called to assist in this endeavor, snarks on the poorly-thought-out idea, but brings her to life anyway. Naturally, after Hercules gets tired of her Clingy Jealous Girl tendencies, she shows off her Yandere tendencies. By the end of the episode, Aphrodite gives her a real personality... one that just doesn't care much for Hercules at all.
In Ben 10: Omniverse, Princess Looma comes off as one of these thanks to being raised in a Proud Warrior Race Guy society. Her attempts to make Kevin honor their engagement are extremely violent and destructive. This quote says it all:
I will fight for your love, even if I have to break every bone in your body!
While not murderous (probably), Sierra from Total Drama World Tour shows many signs of this towards her crush Cody.
The Problem Solverz: Katrina Rad is obsessed with Roba and stalks him in "Magic Clock". She then steals a time-freezing clock so the two of them can be together for eternity and tries to marry him against his will. When Roba refuses to kiss her at their wedding, she threatens to rip a hole in the fabric of the space-time continuum.
Harley Quinn from Batman. She made her first appearance as The Joker's girlfriend.
Also, Firefly in "Torch Song." He had a shrine to the singer Cassidy in his apartment.
The Mad Hatter, for Alice. He put her boyfriend under mind control and made him break up with her.
In one episode of The Boondocks Granddad meets an incredibly hot woman over the internet. We later learn that she's also a martial arts master Raised by Wolves, has a very bad history with previous boyfriends (whom she killed) and has a friend who gives her terrible advice. The episode ends with the Freemans managing to calm her down after which, her friend drives her to kill herself, to the relief of the other characters.
Amora the Enchantress in The Avengers: Earth's Mightiest Heroes is this to Thor. She takes out her anger from being rejected by him in battle with Thor and his allies frequently. This follows along with her comic book character.
An episode of Regular Show has Starla, Muscle Man's old girlfriend. She falls in love with Mordecai, and he starts dating her just so he can get her and Muscle Man back together. But when Mordecai can't take it anymore and breaks up with her... well...
In Season 2, we are treated to Giffany, a love interest in a Japanese Dating Sim who turns out to be sentient. When Soos buys and plays the game, she becomes incredibly possessive of him, believing him to be her boyfriend and going on a rampage when he goes out with a "real" girl.
Cyclops was this to Jean Grey on Wolverine and the X-Men due to the unhealthy nature of their relationship. And Jean is this in turn to him somewhat in the finale.
My Little Pony: Friendship Is Magic has one episode (The Best Night Ever) where Fluttershy of all ponies turns into a Yandere for a bunch of animals. She at first tried being nice and encouraging them to come out, but then she snaps and sets up a few traps. Her frustration leads to her screaming a pretty yanderific line.
"You're going to LOVE ME!"
Western Animation/my life as a teenage robot has a character Sheldon Lee who was first introduced in (attack of the 5 1/2 foot geek) who is unhealthy obsessed with Jenny though he hasn't become homicidal in the show due to it being a kids show, he is shown to stalk her regularly (and thinks its normal which could show he's a little sick in the head) has a shrine to her and is willing to go to extreme lengths to have her, and NEVER gives up. And even though he isn't a skilled fighter physically he is extremely intelligent perfectly able to create a weapon that could deal great damage to anyone who gets in his way of his affections for Jenny.
Western Animation/Dexter's Laboratory Mandark seems to be this for Dee Dee shown by his obsessive love for her and the fact that he never gives up even though she has no feelings towards him and even hates him. Mandark is defiantly a evil stalker for Dee Dee even though he would never intentionally harm her as seen from the comics were he accidentally kills dee dee and falls into severe depression and even lets dextor kill him.