This is based on opinion. Please don't list it on a work's trope example list.
Nightmare Fuel / Justice League
Really, the nightmares started from episode one. However narmy the Imperium may have been, its attack was anythingbut.
For that matter, there's a certain shock to seeing what appears to be an ordinary guard dog start walking straight up a wall as it transforms into something entirely different.
J'onn's torture. It, quite frankly, looks like someone digging their fingernails inside someone's face. All bloodless, though. Even so, it's terrifying. Matters are made worse when he is essentially ABSORBED into a different Martian.
The part where he deals with the Imperium—grabbing the tentacles still embedded into his own body and dragging it into the sunlight...we see the Imperium's outer layers starting to form bubbles that quickly burst, like a hyper-skin irritation, like boils, and as it screams, J'onn delivers the line: "You live in the shadows, and shun the light. Why? ''Does it burn your putrid skin?"
The Justice Lords' Superman's Psychotic Smirk after he killed President Luthor, all with creepy background music.
Also, Batman asking "Do you smell something burning" right before a scene cut to where we see the burned and melted down remains of the Oval Office desk and Superman standing next to it, which lets us know that, yes, he did blast Luthor.
What he says to Batman and Wonder Woman right after makes it a LOT worse. "I'm great!"
Lord!Superman lobotomizes Doomsday. The matter-of-fact way in which he does it and the speed in which Doomsday goes from being relatively well-spoken to drooling are equally chilling.
In a later episode, the real Superman is actually driven by desperation to attempt the same maneuver, but due to Doomsday's Adaptive Ability, this no longer works. Thus three bits of Nightmare Fuel in one scene: 1) Superman can do that, 2) And he will if he's desperate enough, 3) And on some enemies, it still won't be enough.
Lord!Batman. The biggest nightmare in all this is that Batman himself has turned over to the dark side. That in itself is scary enough (this is the guy who sees the Justice League, all of the Justice League, as a countermeasure to himself). But what's scarier is that after Superman vaporizes Lex Luthor, all he offers is a disinterested "Well. It had to be done." He has no sadness, no revulsion towards Superman breaking one of their central tenets, in fact, he sees Superman killing Lex Luthor as a necessary move. Think about this for a second. The guy whose one rule is Thou Shalt Not Kill approves of murder when it is necessary.
Then there's Flash telling Lord!Superman that he couldn't kill him, since the death of his Flash was what started everything. Lord!Superman shrugs and essentially says, "Well, now I don't care." and is ready to fry him.
Being an homage to the Cthulhu Mythos, "The Terror Beyond" is stuffed to the gills with it.
What happens to the King of Kaznia, who is poisoned and paralyzed by Vandal Savage as part of his plan to take over the country. He's unable to move and is forced to watch his daughter get married to the man responsible for his predicament. Made worse, since we never see what happens to him, it's heavily implied that he most likely perished when the rail-gun was redirected to destroy the castle!
Which if that wasn't bad enough, also means that Batman of all people, almost killed Princess Audrey's father with a big honking space-gun! When we saw the people running out of the castle, you can see one of the soldier's carrying someone dressed in purple. The king was wearing purple pajamas. So, the king didn't actually die in the explosion. But what if the guard hadn't been on the watch?
The look of horror on Flash's face, when he briefly got spaced.
Doctor Destiny and Ace of the Royal Flush Gang are especially fun cases in this regard. Their common special ability: Mind Rape. Which is to say, they are in themselves Nightmare Fuel in episodes that use Nightmare Fuel as a plot point.
The ending where Doctor Destiny has gotten everybody but Martian Manhunter and Batman to go to sleep. They show each of the heroes going to sleep, while playing a toned-down music box in the background. Finally, you hear Doctor Destiny softly chuckling. Not an Evil Laugh, just a... chuckle. Worst of all, Superman goes to sleep, and Batman calls him moments later, trying too late to warn him. End of episode.
Penny: John, please. John: You know, I never liked that name, it's so...ordinary, Especially for someone who has such big things in store. You know, a destiny. Oh, destiny! I like it: Dr. Destiny. What do you think? Penny: You're crazy. John: I think you're finally seeing the real me. (clothes turn into spooky robes) And now that I'm a doctor... (face falls off, revealing a skull) ...I think I'll perform some surgery.
Destiny looms over the Flash when he enters his dreams and says a chilling line.
Flash: I get it, I'm dreaming, all I have to do is close my eyes and I'll... (closes eyes nothing happens) Dr. Destiny: Wake up?
While you're inside the dream he traps you in, you can't wake up, and the torture the victim goes through causes their heart to accelerate to dangerous levels until it eventually gives out. Batman and Martian Manhunter make every attempt they can to wake the others, and Martian Manhunter in the end resorts to entering the dreams to save the others, with nothing else working.
The cannibalistic kids in Flash's nightmare? They had nothing to do with the nightmare per se, but it was so out of nowhere... They got to be even scarier than the context of the scene. Good thing he has Super Speed. Or not.
Superman accidentally destroying Metropolis and snapping Jimmy Olsen's spine in his nightmare should also count. As well as accidentally frying Lois with heat vision at the very beginning. Both horrifying and heartbreaking, especially given what we see of his reactions; of all the Leaguers, his is the most outwardly pronounced. After he accidentally kills Lois, we cut to his apartment where we see him screaming and flailing around as if in agony.
Green Lantern's nightmare is another one that's very effective in how relatable and relatively subtle it is. Imagine coming home, only to find that it isn't home anymore, that no one recognizes you and that the people you care about are terrified of you for reasons you don't understand, that even your surroundings have changed.
Dee's fate at the end is creepy. He's lying in bed, seemingly catatonic and humming Frere Jacques. Who knows what he's seeing...
Even though he's on the side of good, hearing Batman's quiet humming in his head has a creepy sound to it, like you can hear him about to snap underneath from lack of sleep, and yet he's so calm...
Well, actually, that was probably him faking it, since he replaced the torturer easily enough. Shape-changing, Super Strength, phasing...he's equipped for this. Doesn't make his screaming any less creepy. Seriously, something about J'onn's voice makes any screaming, real or faked, kind of scary.
Given that the torturer looks weak when the guards find him strapped to the table, this leads to the very real question of whether some of those screams might have been from J'onn torturing him?!
Speaking of "Hereafter," there's also Vandal Savage himself in said future. There's another reason why he's become much more sedate and friendly: countless generations of living with the consequences of his actions had made him boredwith insanity and madness. Which makes the way he chirpily recalls how he killed the Justice League as if he remembering a sports game seem so unnerving.
One of the scenes in the Justice Lords episode "A Better World" has the League visit the alternate Arkham. Many villains, particularly Batman villains, are acting very much out of character. Then, you notice two small dots on all of their heads. Then, you recall what Lord!Superman did to Doomsday. Oh, and Scarface's dots are on the dummy, not on the ventriloquist.
The first person who is revealed to have been lobotomized by Lord!Superman is the Joker; who is now the secretary of Arkham Asylum. Hearing his voice and seeing his appearance to be so calm, almost serene, it really drew an unsettling feeling considering we're used to seeing him as a psychopathic, murdering clown. Who would've thought one way for the Joker to be scarier was to calm him down?
Flash's scene with Poison Ivy. Ivy's a real unpleasant, fanatical piece of work, in the DCAU and without, but seeing her like that...content, calm, friendly, and humming as she cuts the the buds off of flowers...seems just fundamentally wrong. Creepy, you might call it. Especially when you think about Ivy's obsession with plants—she nearly goes into conniptions if anyone so much as touches one of her plants. For her to go so far as to actually be "hurting" plants...it's so thoroughly not her. Even when a decapitated robot head falls right next to her, she just continues on with her work.
At the end of "For the Man Who Has Everything," Wonder Woman puts Mongul's Lotus-Eater Machine plant on him. Right before the episode ends, we get to hear painful screaming and horrible destruction, presumably all from Mongul's fantasy. And Superman wasn't the only one screaming.
This episode deserves mention for when the plant is put on Bruce—we get to relive the moment his parents were shot (in black and white, to give it a proper Film Noir feel), but in his dream, his father fights the criminal and continues to punch him. But as Wonder Woman tears off the plant, we see Bruce's father be overpowered by the criminal, zoom in on young Bruce's face turning more and more horrified as we finally hear a combination of a tearing sound and a gunshot when the plant is removed...it's truly horrifying. Made worse in that when the plant attaches to him, we see the only genuine, contented smile Batman ever has in the entirety of the Justice League run.
Wonder Woman falling victim to a No-Holds-Barred Beatdown by Mongul was pretty unsettling to watch. We have seen Wonder Woman take a beating before, but this episode in particular makes it worse than the others. For instance, you had Mongul coming close to actually killing her. She had to crawl to Bruce to save him from the plant because she could hardly stand after the beating she took from Mongul.
The end of "Kid Stuff," where Mordred's spell to get rid of all grown-ups is reversed when Mordred is tricked into turning into an adult himself, and his mother Morgan Le Faye notes that the spell giving him eternal youth is now broken, leaving him only with eternal life. As if simply thinking about the ramifications of this isn't enough, the last shot of the episode shows Morgan tenderly wiping the drool from the mouth of a glassy-eyed old man.
The episode "Dark Heart." A planet being devoured piece by piece, the inhabitants mostly being able to do nothing as they see the wave of machines sweeping towards them.
In "Destroyer," Darkseid describing what he's going to do to Superman while he's writing on the ground in pain from the Agony Matrix. Specifically, Darkseid pulls out a Kryptonite knife and tells Superman that he's going to carve out his heart and place it on a pike as a trophy.
The portrayal of Darkseid in the DCAU is often seen as the definitive portrayal of his character (outside of comics) and for good reason: he is a terrifying character. Not just because he's the only one who can take Superman at his full power, but because he is relentlessly evil. Other villains had reasons that you could like them; at least Lex Luthor was suave, and at least The Joker had a happy attitude and comic timing. Darkseid is not that kind of villain: he is the absolute embodiment of evil.
To give a sense of how terrible Darkseid is, he is the only villain that Superman actively tries and wants to kill.
A rather small one, but the Justice League women being pitted together in "Grudge Match." Huntress and Black Canary manage to free Shayera and Vixen pretty easily, but then they find out they have to fight Wonder Woman (who could match Superman in terms of power). Their reactionis entirely justified. During a rather brutal fight, a few of the League girls come frighteningly close to a bloody death. Hawkgirl almost smashing Vixen's head with her mace, for example.
Also, the way Wonder Woman over-powered all four Leaguers and took absolutely everything they threw at her like it was nothing. Then there's the way she was about to kill the badly-battered Shayera and Vixen by crushing their skulls together. More terrifying is the fact that she was completely unaware of what she was doing and would never have remembered it had she been able to follow through.
The vision the Question sees while being TORTURED in "Question Authority" is pretty chilling.
The Question being tortured period was chilling.
He was missing for almost a week before Huntress tried to get Superman's help. Even then, nobody in the League noticed that he was missing.
Static's howl after getting sucked into a spatial rip was rather chilling, as was Terry's death by the Dee-Dees. More chilling was the fact that the universe was collapsing all around them and they were all completely helpless to stop it.
Batman trapping David and Enid in a time loop to ensure that David never enacts the chain of events that occurred over the episode. That is one chilling And I Must Scream fate.
It's easy to forget exactly how powerful the Martian Manhunter is, since he spends so much time being The Aloner. Then Task Force X sneaks into the Watchtower, makes off with the secret weapon they've been sent for, mops the floor with several unexpected obstacles, and suddenly comes face-to-face with That One Boss: J'onn. And suddenly he's really creepy.
In "Flashpoint," Lex Luthor hijacks the Watchtower's Binary Fusion Generator, forcing it to fire on an abandoned Cadmus facility. When the gun goes off, the scene cuts to a peaceful suburban neighborhood...that suddenly goes very quiet as the sky starts to light up. Then the blast wave comes and nearly levels the city, followed by a mushroom cloud. Aside from the fact that, somehow, nobody (onscreen) is killed, and there's no residual radiation, the entire sequence is frighteningly reminiscent of a nuclear attack.
That's right, kids! Death from above, with no warning, no escape, and no explanation! It could happen to you before you finish reading TVTropes!
The ending of "Hearts and Minds." Despero's army is turned into trees, all the while they are aware of this. Their faces as this happens tells everything you need to know. Especially chilling one a close up of a face with face stuck in a scream, while only one eye is left open...
The scene in which Despero tries to Mind Rape Hawkgirl which causes her to scream in agonizing pain that grows louder and more intense which is pretty terrifying until Green Lantern saves her.
Galataea's Family-Unfriendly Death (Supergirl frying her with the reactor), as well as a good shot of her twitching corpse!
As a clone of Supergirl, she might have survived...still disturbing, though.
Seeing Amazo tear through the Justice League to get to Luthor. In space, he destroyed a fleet of Javelins and defeats nearly dozens of heroes including Green Lantern, Dr. Light, Captain Atom, and Superman himself. In the sky, he goes through the second wave (Supergirl, Fire, Red Tornado, and Rocket Red) like tissue, during which he splits Red Tornado in half and destroys himnote During his first viewing of this episode, this troper was not aware of Red Tornado's background and thought he was human, making it worse. He doesn't even try in the last wave (Wonder Woman, Flash, Steel, and Ice). The thought of something that powerful existing is horrifying. Dr. Fate was not kidding when he claimed Lex was saving the world.
J'onn is a master of subtle Nightmare Fuel in this series. Take his mental interrogation of one of the Thanagarians; he couldn't normally read their minds due to mental defenses.
And then in Hunter's Moon we see the consequences of this interrogation. Said Thanagarian is now a babbling fool who has been Driven to Madness by J'onn.
He gets a great one during the series premiere, tricking a pair of white martians into cornering a few league members so he can get the drop on them from behind. He puts an intangible fist through each of their chests and goes solid. Ouch.
While generally amusing, the entirety of "Legends" has some extremely creepy subtext; indeed, the fact that the arc is so bright and sunny makes it all the more frightening when you put together the pieces. The ideal, idyllic 50's comic books fantasy world was created by a young child who had to experience his entire world go up in flames when a nuclear war broke out, was mutated by the fallout into some...inhuman...cancerous...thing, and was driven so insane that the only he could cope was by using his newfound psychic powers to create an eternal happy dream world where none of this ever happened.
Tala's screams as she's being used up in Luthor's attempt to revive Brainiac in "Alive!." (The show-runners even admitted they were unsettled by this, even after having toned it down considerably from the original recordings.)
Immediately before this, when Tala begs Lex to spare her for betraying him to Grodd, she tells him she's "a sick person." When she realizes he planned on using her up even before she betrayed him, he coldly replies, "I'm a sick person, too."
And she ends up releasing Darkseid, endangering Earth, just to screw Lex out of possessing Brainiac and for spurring her.
Grodd getting spaced in this episode was pretty disturbing, as well.
Also from that episode, Killer Frost kills half of the Legion of Doom, ON SCREEN. Just to get back in Luthor's favor.
The reveal of the real world in "Legends" is chilling when you consider how close our world has come to that and that it could still end up as such.
There is a (relatively) minor one in "Eclipsed" with the soldier who is possessed at the beginning: this guy wakes up and learns that he killed his whole unit, with whom he seemed rather close. Especially since the last time we see him in the episode he's still held responsible for it.
The nerd who performed the satanic ritual in "Wake the Dead" definitely got killed by Solomon Grundy.
The scene in "Epilogue" involving eight-year-old Terry and his parents walking from a movie and as they start to head home, The Phantasm is shown and begins stalking them from behind. Upon getting closer, she raises her hook hand, moments away from killing Terry's mom and dad, all to give Terry the motivation he needed to become Batman. Even more Nightmare Fuel when you learn that the Phantasm was ordered to kill Terry's parents so Terry could experience the same pain that Bruce did so he'd take up the mantle and was real close to doing so.
Also a rare case of Even Evil Has Standards being invoked to avert Nightmare Fuel. The Phantasm is willing to murder unsuspecting strangers, but - when it comes right down to it - can't bring herself to traumatize an eight-year-old for life.
Amanda Waller, bad enough she proved she knew Batman's secret identity the first time they met. But every single time she talks about the clones or mutated soldiers Cadmus created, she's always got one of the nastiest, evil grins ever seen. It's like she doesn't even think of any of them as people!
Panic in the Sky. Just...just Panic in the Sky. The League just lost control of their Kill Sat superweapon that they save for emergencies like the events of Dark Heart, which they fear may have killed hundreds if not thousands of civilians. Understandably, the President is pissed about this and the founding members of the League, sans Batman, decide to submit to government custody until they can sort it out. While they're gone and the Watchtower is still out of commission, Cadmus sends Galatea and an army of Ultimen to attack the Watchtower and kill—not arrest, not defeat, KILL—every last person on the Watchtower. That includes the non-powered civilians who run the Watchtower's everyday systems. In particular, we're treated to a group of those non-metahumans hiding in a safe room when one of the Shifter clones rips the door open, then transforms into a Tyrannosaurus rex. The workers are clearly ready to fight for their lives as they charge at her and the Ultimen are eventually defeated, but one shudders to think about what probably happened to them.