Fillmore! had a fantastic satire of this. There was a nameless protester who held up a sign that said A.C.R.O.N.Y.M. It stood for "All Creatures Really Only Need Your Mercy."
As referenced in this trope's explanation, COPS, the '80s cartoon about a futuristic police force whose name stood for Central Organization of Police Specialists.
In the Venezuelan Spanish dub of the series, while the acronym is still the same, it was translated in Spanish as Comando Organizado de Policias Superiores (Translated as Organized Squad of Superior Cops)
Darkwing Duck parodied this with SHUSH, which did have a meaning, but it was so secret that nobody knew what it was. SHUSH's main antagonist was the evil counter-agency FOWL, The Fiendish Organization for World Larceny.
In DuckTales there's an episode that takes place on a robot planet that's ruled by a Humongous Mecha named Master Electronic Leader.
In "The Great Patty Caper" when SpongeBob and Mr. Krabs are debating whether to add land salt or sea salt in the patties. Mr. Krabs brings up the acronym SCABS for Salt Comes After Barnicle Shavings. Then he brings up the acronym LESIONS, but we never know what it means. Spongebob asks about PUSTUAL, then Mr.Krabs says "Heavens no! That's disgusting..."
Also spoofed in the informational video episode, in which the acronym POOP stood for People Order Our Patties.
The titular heroic organization on M.A.S.K. stood for Mobile Armoured Strike Kommand. The criminal organization of that show, meanwhile, was the Vicious Evil Network Of Mayhem.
Futurama had Fathers Against Rude Television in the episode "Bender Should Not Be Allowed On Television". ("And for what? A cheap laugh? That's not what F-A-R-T is about!" "No sir! Not us FARTers!")
The show also had Mankind for Ethical Animal Treatment, and the UN-like interplanetary alliance known as the Democratic Order Of Planets.
A special feature on the Bender's Game DVD has the anti-piracy organisation Downloading Often Is Terrible.
In "31st Century Fox", Bender starts a group against robot animal cruelty called, "Bender's Animal Robot Front."
"Saturday Morning Fun Pit" has G.I. Zapp's team go up against the terrorist group, Acronym, ACriminal Regiment Of Nasty Young Men
An episode of Dexter's Laboratory had Deedee and her friends, inspired by a McGruff knock-off at a school assembly, form a neighborhood watch club called "G.I.R.L. Squad", which purportedly stood for Glamorous Investigators Ready to Lick Crime.
Used for the titles of every episode of Codename: Kids Next Door as an instance of Idiosyncratic Episode Naming. (Except for one of the two stories in the pilot episode.) In addition, every gadget used in KND has an acronym for a name (except the one episode that didn't have an acronym for a title), which is usually explained shortly after said gadget is introduced.
Then there's "The Grim Adventures of the Kids Next Door", where this practice is lampshaded with the MANDROBOT (Monkeys and Nice Doggies Relax on Bellies of Turtles).
Mandy: "What kind of acronym is that?"
Numbuh 3: "Well, maybe if you weren't in such a rush, I could've come up with a better one."
Another episode played with it by having Numbahs One and Two convinced (in an Epileptic Trees kind of way) that bras are a secret teenager weapon, inventing the acronym ("Battle Ready Armor") on the spot. Turns out they're 100% correct.
One episode of South Park took an existing organization called "NAMBLA" (North American Man-Boy Love Association) and created another "NAMBLA" that stood for National Association of Marlon Brando Look-Alikes.
The main antagonist of Birdman was the terrorist organization F.E.A.R., which the characters could never seem to decide to pronounce letter-by-letter or just say "fear." Either way, it was never revealed exactly what F.E.A.R. stood for.
The Irken invaders in Invader Zim are provided with "SIR"s, or Standard-issue Information Retrieval units. Zim, ever getting the short end of the stick, received a "GIR" model. When asked what the 'G' stood for, GIR cheerfully replied that he didn't know, then began to beat himself on the head while giggling maniacally.
In another episode, Professor Membrane invents a machine called a Perpetual Energy Generator.
On The Boondocks, Huey explains in an interview that he is the creator of over a dozen radical leftist groups, including Africans Fighting Racism and Oppression (AFRO), the Black Revolutionary Organization (BRO), and the Black Revolutionary Underground Heroes (BRUH).
On The Venture Bros., the Venture nanny robot H.E.L.P.eR. (Humanoid Electric Lab Partner Robot; Apparently that acronym was so hard to match they had to put an "e" in for aesthetic reasons) and security robot G.U.A.R.D.O.
One word: W.I.T.C.H.. Born of Hay Lin jokingly writing down on her hand the initials of the group after they found out they had magic powers (according to Taranee and Irma, she had been poisoned by the ink of writing on her hand too many times).
The 4Kids dub of Winx Club made a pointed jab at W.I.T.C.H. when Stella wanted to call themselves "B.S.M.T.F.": "Beauty, style, magic, taste, and flair. And those are also the first letters in each of our names."
When the previous team of Guardians is revealed, Hay Lin does it again, and gets C.H.Y.K.N. (pronounced "chicken"). She isn't fond of that revelation.
In the comic book she does it again with the names of the main actresses in a musical whose plot (written by Will) is based on the first arc. The result? M.A.G.I.C.
Also from the Jetix stable is A.T.O.M., standing for Alpha Teens on Machines.
"El Viaje Misterioso de Nuestro Jomer (The Mysterious Voyage of Homer)" had Electronic Automatic Robotic Lighthouse.
To Professor Frink, nerd stands for Not Even Remotely Dorky, "So thank you. Thank you for the compliment".
At a sexual harassment seminar in the Nuclear Plant, the whiteboard in the background reads, "Realize Even Soft-core Pornography Eventually Causes Trouble."
In "The President Wore Pearls", Principal Skinner unleashes operation SLAAAM: So Long Athletics, Art And Music.
One episode shows that Martin Prince has a robot called, Childlike H umanoidUrban Muchacho.
Subverted with Lindsey Naegle's anti-youth group, which had a pretty lame (and unnecessary) acronym for a name, SSCCATAGAPP ("Singles, Seniors, Childless Couples And Teens And Gays Against Parasitic Parents"). Of course, seeing as her goal was to deprive Springfield of all child-friendly things, maybe that was the point; she had No Sense of Humor and didn't like anyone else having it.
Transformers Animated has a train invented by Professor Sumdac called the "Fully Automated Rapid Transit System". According to the series creators this was entirely unintentional.
This was lampshaded during a script-reading where Sumdac showed off his "Bi-directional Unified Transit Terminal, which would let the Autobots get back to Cybertron by putting a crack in space. Everyone else makes several veiled jokes about it, while Sumdac apparently remained entirely oblivious.
Lugnut's Megaton Punch was given a name in the fandom before later being made official. It's called the Punch Of Kill Everything.
There's also the main organization World Organizatin Of Human Protection, which a villain try to change the past nearly turned into World Organizatin Of Harming People. Another episode had a group called G.O.O.P.E.R, which later revealed to be an acronym for Global Organization Of Pilfering Extraterrestrial Resources, and apparently nobody but the members of the group knew this.
This often (but not always) happens with the girls' gadgets. The most recurring one is the "UPWATI"note "Up-what-y?" "Not 'up-what-y', 'UPWATI'.", or Underwater Power Walking Apperatus That's Inconspicuous.
The episode "Interview with a Platypus," with Dr. Doofenshmirtz planning to flood Danville so everyone will have to buy his latest invention: the Buoyancy Operated Aquatic Transport (Pronounced "BOE-at"). Perry thwarts this plan by opening the Metropolitan Oval Aquatic Trench at just the right moment.
That spells moat [pronounced properly] I never realized that -Doofenshmirtz lampshading for us again
From "Backyard Aquarium":
"Say hello to the Platypus Secret Agent Arrival In Order To Foil My Evil Plot Capture-inator! Or as I like to call it, my P.S.A.A.I.O.T.F.M.E.P.C.-inator." and "Behold! The Hot Dog Vendor Revenginator! Or, my H.D.V.R., er, er, inator. I have to work on my acronyms." -Doofenshmirtz, again lampshading this trope
Subverted with Perry's spy agency, "The Agency" officially O.W.C.A (pronounced in a similar fashion to orca) or "The Organization Without a Cool Acronym." Yes, Yes they did just go meta with their lampshading, somewhat of a Running Gag.
"Owca" is Polish for "sheep", so it kinda makes sense as a name for an agency that hires exclusively animals as its agents.
"Comet Kermillian" gives us Dr. Doofenshmirtz's Steak Containment Unit, pronounced "SKUH"... And then later on "SKOO".
Gargoyles: The Goliath Chronicles had a pro-integration group called People for Interspecies Tolerance. What made it brilliant was the fact that their enemies were called the Quarrymen.
The Canadian show The Wrong Coast had a sketch that combined S.W.A.T. with Antiques Roadshow to create S.W.A.T.A.W.A.T.A.T.A.A.: Special Weapon And Tactics As Well As The Ability To Appraise Antiques
Rocko's Modern Life had an episode in which Heffer shows Rocko his ticket to the All Scottish Show. The acronym-making first letters are large red capitals, bigger and colored differently than the rest of the letters.
In the origin story episode of Pinky and the Brain, when Project B.R.A.I.N., (Biological Recombinant Algorithmic Intelligence Nexus) the gene-splicing program that gave Pinky and Brain their intelligence, appears to have failed, the military superior to the scientist working on the program angrily tells him that he'll now be working on "Project B.R.A.W.N." When asked, he says that he doesn't know what it's for; he just made up the acronym on the spot.
In "Das Mouse", while commandeering a submarine, Brain asks Pinky to randomly change the radar signal beacon letters so that they couldn't be followed. Naturally, Pinky changes it to "NARF", unfortunately a government facility translates it to Nuclear Attack Readiness Formation.
The Marvelous Misadventures of Flapjack featured the main characters in a runaway trolley. Cut away down the harbor to the resident Gadgeteer Genius showing off his latest ramp-like invention: "I call it R.A.M.P.! Radical Ascension—"Then the trolley smashes right through it.
He also created M.U.S.L. (pronounced "muscle"), short for Magical Under Sea Lair.
In Inspector Gadget the criminal organization MAD either stood for Mean And Dirty or the Malevolent Agency of Destruction. It was never officially revealed what the name stood for, or even if it was an acronym at all.
Prior to that the Tom Of T.H.U.M.B. segment of the 1966 King Kong cartoon (Tiny Human Underground Military Bureau) had a criminal organization called "MAD" which meant Maladjusted, Anti-social and Darn mean.
Played with in Donkey Kong Country, as K. Rool describes his latest weapon, the K.Rool to Crystal Coconut and Back Missile.
K.Rool: I call it the KCC-BM!
Klump: Um, "cuss-boom?" I think you need to recruit a few more vowels, sire!
K.Rool: You idiot...
During World War II, Warner Brothers animators produced a series of shorts for soldiers to educate and inform them on military matters. The cartoons starred the "world's worst soldier", Private Snafu. S.N.A.F.U. is of course a military acronym; Situation Normal, All FuckedFouled Up.
The 2012 cartoon turns Baxter Stockman's iconic mousers into Mobile Offensive Underground Search Excavation and Retrieval Sentries.
Leonardo: Seems kinda forced.
American Dad!: Stan comes home and laments that everyone is making fun of him for having accidentally pooped in a neighbor's pool. And with the worst possible timing; Bullock just assigned him to the Tactical Urban Response Division! He briefly flashes his coat showing the acronym before it dawns on him.
One Recess episode where the government cancels recess in a poor attempt to boost test scores, they re-introduce it under the acronym Reversing Effects of Continuous Educational Stress Syndrome, or "reckess" as it is pronounced.
In another episode, Timmy's enemies team up to form the League Of Super Evil Revenge Seekers.
Tuff Puppy is fond of these, namely the names of headquarters.
T.U.F.F. = Turbo Undercover Fighting Force
D.O.O.M. = Diabolical Order Of Mayhem
G.L.O.O.M. = Genius Larry's Order Of Mayhem
F.L.O.P.P. = Fiendish League Of Potential Perpetrators
In The Angry Beavers episode "Salmon Sez", the Beavers' dam is blocking a group of salmon from migrating, thus preventing them from spawning. Not knowing what "spawn" means, Daggett comes up with his own definition in acronym form: Smashing Property And Wamping Norb. He then comes to the conclusion that the salmon want to "SPAWN [his] brotherto death!"
On Disney's Doug, there's a competition to name the new school, and Doug's suggestion is Learning Is Educational Middle School. It's not until he gets the acronym put on a sweatshirt for the competition that he realizes the Unfortunate Implications.
In one episode of Code Monkeys, Dean's fraternity is revealed to be Alpha Sigma Sigma. It's possible that the fraternity picked the name solely for the acronym.
From The Critic, during a meeting with Hollywood agents.
Agent: Jay, ever since we at Preferred International Talent Brokers merged with United Labor Leading Specialists...
Agent:(turns around and looks at the giant P.I.T.B.U.L.L.S. sign) Hey look, that does spell pitbulls.
Agent 2: Well what do you know. Well we are aggressive.
(Agents all laugh which quickly turn into dog-like growls.)
Diffusion, information, et Communication: better (and later) known as "D.i.C" (later "DIC").note The company was originally founded in France, hence the expanded French name. While the latter two are obvious, "diffusion" refers to broadcasting. The company eventually discontinued the original name, removing the periods in the process.
Godzilla: The Series had S.C.A.L.E. or Servants of Creatures Arriving Late to Earth, eco-terrorists believing the mutations living on Monster Island was just evolution saying humanity's time was up. Leader of said group was so outside her gourd that she wouldn't have minded being eaten by anyone of them.
And of course there's also the Five-Man Band known as H.E.A.T. or Humanitarian Environmental Analysis Team (originally proposed as High-performance Environmental Attack Team), and their Robot BuddyButt Monkey, N.I.G.E.L. or Next-millenium Intelligence Gathering Electronic Liaison. Randy promptly lampshades with "Shouldn't that be 'NMIGEL'?"
One of the Monster of the Week is called a Dragma, but its name's origins comes from the Democratic Resurgence Against a Global Mechanized Armageddon.
Danger Mouse: In "Journey To The Earth's 'Cor!", DM finds the room with the machine holding his car frozen in mid-air, inside a door marked "C.H.M.F.F.G.," which Penfold surreptitiously pronounces as "chimuffguh." DM says it's for Car Holding Magnetic Force Field Generator.
"One Of Our Stately Homes Is Missing" had B.L.E.E.P., which was the Building Location and Emergency Extradition Platoon.
TOM, the host of Toonami, is short for Toonami Operations Module.
The My Little Pony: Friendship Is Magic episode "MMMystery on the Friendship Express" has Pinkie Pie guarding Mr. and Mrs. Cake's entry in the Equestrian National Dessert Competition, a cake known as the Marzipan Mascarpone Meringue Madness, or "MMMM" for short.
Archer works as a spy in I.S.I.S. for International Secret Intelligence Service. Then there's their O.D.I.N. for Organization of Democratic Intelligence Networks.
The Wingdinglish inscription inside the titular artifact in BIONICLE: Mask of Light reads Technic Organic Apps One Destiny. Toa are the heroes of the BIONICLE world, and this was likely an Easter Egg referencing to the old "Six Heroes — One Destiny'' tagline. Of course, when the characters read it, it becomes a long-winded text of made-up words.