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Danganronpa

  • Danganronpa: Darkened Hope: Aiko lets one out in the first Class Trial when Monokuma begins messing with her.
    Aiko: Ahhhh~! Aiko doesn't wanna be suspiciooooouuuuuus!!!
    Monokuma: You're suspicious, Aiko! Like really suspicious! Like super ultra major suspicious!
    Aiko: Nooooooooooo!

The DCU

  • In Hellsister Trilogy, Linda Danvers introduces her new beau Dev-Em—who is secretly a 31st century Legionnaire—to her landlady Ida Berkowitz, who grows concerned with Linda dodging every question regarding her new boyfriend's mysterious personal life. So, when Ida asks if Linda could take her to his workplace...
    Ida: Seems to be hiding something. 'Security consultant'? So all right. Maybe he is. Maybe he's got to be Mr. Secret to do his job. You sure this is really what he does?
    Linda: Sure as I can be.
    Ida: Like how? You been to his place of business? Could you take me?
    Linda: NO! I mean, yes, I've been there, and no, I couldn't take you. He made an exception for me.
  • This is Damian's reaction when he's told Bruce and Selina are expecting a baby, early in Batman: Grudge Match. At the start of the next chapter, Alfred is sweeping up glass from a window, implying Damian's scream shattered it.
  • With this Ring... (Green Lantern):
    • Exclaimed by Hector Hammond when Hal Jordan takes advantage of his low mental defenses to escape his death trap and retaliate.
      Then he felt a pressure in his own mind. A pressure which rivalled any to which he had subjected a foe. The mentalist's defenses went into operation, but it was a bit late. After all, when an enemy has transformed his body into an analogue of mental energy and sped up the telepathic pipe into your mind, such an action is closing the gate once the horse has bolted.
      <NO!>
    • Screamed by the Predator when he gets blasted into oblivion.
      A purple burst of light cleaved through the roof of the throneroom, smashed into the force-ball, and crushed it into the Predator's body.
      A light of terror went on in his eyes as he shouted, "NOOOOO!", before finally and totally combusting out of existence.

Dragon Ball

  • In Dragon Ball Z Abridged:
    • From episode 10:
      Goku: Kaioken...
      Vegeta: ...Noooo!
      Goku: ... times...
      Vegeta: No no no no!
      Goku: ... FOUR!
      Vegeta: No no no no FUUUUUUUUUUUUUUUUUUUUUUUUUUUuuuuuuuuuuuuuuuuuuu...!!!!!
    • Additionally, Vegeta's response to being haunted by Ghost Nappa.
    • Plus there's Mecha Freeza's reaction to learning that he blew up Planet Namek and the Dragon Balls from King Cold, in a scene that is very much like Vader's in Revenge of the Sith.
    • "Vegeta, no!" "Vegeta, YES!"

Godzilla

  • Abraxas (Hrodvitnon): Vivienne Graham, one of the two protagonists, has a couple. The first comes when she learns her mother is at Yonaguni and she turns to fear that the Many could still be around, the second instance comes when she learns Ghidorah and MaNi/Elder Brother intend to forcibly use her as a monster Baby Factory as a way to torture her into Ax-Crazy.

Harry Potter

  • Harry Potter and the Methods of Rationality: Early in the story, based on the Quibbler headline about how Harry gets Malfoy pregnant, the author got several e-mails asking if Luna was a seer, and if the story was going to turn into an HPDM bottom!Malfoy mpreg fic. The author regretted that fanfiction.net did not allow a larger font with which to say NO.
  • This occurs twice in Oh God, Not Again!; once by Fred and George when they see the Quidditch field is now a hedge and again by Harry when he finds out Hermione won fifty points (again) after he dedicated himself to losing the House Cup.
  • In 3 Slytherin Marauders young Tom Riddle gives one after the Sorting Hat sorts him into Gryffindor.
  • In Losing Control Voldemort and all of his Death Eaters yell one when Azrael announces that as punishment they'll have to spend the next thousand years serving Muggleborn as house elves.
  • In Harry Potter and the Fold Harry yells one after a nightmare where the ghosts of Cedric and his parents say he should've died in their place and the figure coming out of the cauldron after Voldemort's resurrection is himself.
  • In Nobody Cared Snape yells one during a nightmare about Remus almost killing him during his sixth year.
  • In Emancipation Hermione yells one when Macnair kills Crookshanks.
  • In Hand Delivered Letter Harry yells one when he hallucinates that the troll is his Uncle Vernon.
  • In Snape's Worst Nightmare, chapter 7 ("Them v Us"), Snape lets out a huge "NO!" when Isabella Weasley manages to get the Snitch in a game between the Weasley-Potter team and the Slytherin team. He had bet Harry that he would never punish the Weasley-Potter cousins if they won.
  • In Harry Potter and the Trip to the Past the basilisk yells one when Harry and Snape use roosters to kill it.
  • In Wargs and Peace, when the company Thorin is dead, Fili yells this.
  • In Claimed Molly Weasley yells a ludicrously long one when Ginny kills herself.
  • In The Circle Fred and George yell a deliberately melodramatic one in unison when Dumbledore announces the cancellation of Quidditch at the start of Harry's fourth year.
  • In Harry the Hufflepuff 2 Harry yells one when the conductor of the Knight Bus realizes that he forgot to switch the bus out of "night mode" and all of the beds are replaced with uncomfortable-looking wooden chairs.
  • In Not the Only Redhead Mrs. Weasley yells one when Bill and Charlie are arrested on suspicion of having beaten Harry half to death in Hogsmeade.
  • In The Consort Tournament Dolores Umbridge yells one when Bellatrix Lestrange asks if she wants to "play with her."
  • In It All Started With a Visit to Gringotts Sirius' portrait yells one when it discovers Snape is Harry's boyfriend.
  • In Make a Plan Fenrir Greyback yells one when Dumbledore cures his lycanthropy during a werewolf attack.
  • In Deserving, everyone has No Indoor Voice especially when uttering the word "no", which appears in ALLCAPS as often as not.

Hetalia: Axis Powers

  • Gankona, Unnachgiebig, Unità: After Germany pulled out a Browning Hi Power on Japan and Japan pulled out his katana on Germany, Italy screamed one word for them to stop.
    "NOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!"

Invader Zim

  • Becoming a True Invader:
    • Zim, plenty of times:
      • When GIR being ordered into defensive mode against an angry Gaz just causes him to jump out a window, letting her into the base.
      • When being forced to listen to Tel expositing on his culture.
      • When losing in a game he's playing with Gaz.
      • When the Alternate Universe Keef shows up. The "no" is so big it starts at the end of Chapter 27 and continues well into the start of Chapter 28.
    • The Dibship does this a lot too, in keeping with its melodramatically emo personality:
      • Upon finding that it's been rebuilt even bigger than before (as it now sees itself as fat).
      • When Zim and Dib toss Tak (whom the ship is obsessed with) out of the ship as they're leaving Earth.
      • Seeing that a Goth (namely Kor) is already standing at the tree he wanted to mope at.
    • Zim and Gaz get one in unison when they realize they're stuck together as their ship leaves Earth.
    • Red gets one when his and Purple's last ditch attempt to stop the Employer's fleet from reaching Irk fails.
    • Skoodge, when he's been led to believe that Zim's dead.
    • The Employer upon his final defeat, which trails off as he falls into a portal to an empty void.
  • Invader Zim: A Bad Thing Never Ends: This is Dib's reaction to Zim's new minions all joining his class, with no one seeing through their terrible disguises.

The Legend of Zelda

The Loud House

Marvel Universe

  • In A Prize for Three Empires, Carol Danvers lets one out, combined with remorseful tears, when she finds out Zenith, a Shi’ar guardsman she killed during a battle, was her friend Raza’s brother:
    Warbird looked up, her eyes closed and tearing, her fists clenched, and shouted one great word that filled the cabin.
    "NO!"
  • In X-Men: The Early Years, Iceman gets one:
    "Are we there yet?" Bobby Drake whined for about the thousandth time. Scott Summers just sighed. Hank McCoy rolled his eyes. Warren chuckled humorlessly to himself and Jean was going to start losing her temper.
    "NO!" Was the reply that quickly came from all his teammates.
  • X-Men 1970: Uttered by the leader of an extremist group when Cyclops asks if fear of being conscripted is his chief motivation.
    Cyclops: So, you're doing this because you're afraid of the draft?
    Carter: NO!

Miraculous Ladybug

  • Feralnette AU: Lila gets one during Lady Clarity's attack when Adrien demands to know if she's really allied with Hawkmoth.

My Little Pony: Friendship Is Magic

Neon Genesis Evangelion

  • A Crown of Stars: Asuka combined this trope with Rapid-Fire "No!" in chapter 5. When Asuka insists that she does not need anyone and she has an agreement, Daniel points out that it is very fragile and she fears that it will not last... And then what? She will try to kill herself again?
    She hadn't cried, not even the day they buried her Mama. She would not cry now. She would not. 'Don't take him away. I don't want to be alone. I don't want to be alone.' "I don't... ...need anyone. My arrangement-"
    "Is a hollow shell, that you both fear and expect to collapse at some point. Then what? Water again? Or this time the barrel of a gun in your mouth?" Daniel said quietly, his words hitting Asuka harder than if he’d been shouting them. "Or a twist of hemp like your mother, feet in the air?"
    "NO! Nononononono! I...I...STOP IT!"
  • Advice and Trust: In chapter 4 Asuka shouts one when a classmate severely misunderstands Rei:
    Rei:"Though I believe this bond predates that session. Ikari is the first person Pilot Soryu ever let inside her Entry Plug."
    Tanaka:"Is... is she talking about-"
    Asuka:"NO!"
  • Evangelion 303: Asuka screamed it several times after Jessika told her that she could not be with her yet.
  • HERZ: Exclaimed by the SEELE members when the Children took control of their newest MP-EVAs.
  • The One I Love Is...: When Shinji saw the MP-EVAs had chopped Unit-02 into pieces and possibly killed Asuka he screamed a very big and anguished "no":
    I opened my eyes and then saw a scene that will probably haunt me the rest of my life. I saw nine white EVAs, circling around what was left of Headquarters. And in their hands, or even worse, their mouths, they held the remains of what had once been Unit-02.
    "NOOOOOOOOOOOOOOO!!!!!!!!!!"
  • In Wake, Shinji shouts a very loud "FATHER, NO!" when he wakes up from a nightmare relating to the end of the world.

PAW Patrol

  • In Face The Vax Everest shouts this when Jake drags her to the vet's office.

Sonic the Hedgehog

Yu-Gi-Oh!

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