Season 1
- Cheryl Hole's name is an clear reference to Cheryl, her biggest idol who she got the chance to meet during one of the season's episodes.
- Sum Ting Wong's name is a reference to a viral video
of a news anchor who was tricked into reading fake names for victims of a crashed flight. - Vinegar Strokes enters saying "I didn't become a little bit of a slag, I became a total slag!", a famous line said by Kat Slater from EastEnders.
- As a reward for challenge wins, contestants receive "Ru Peter Badges".
- The acting challenge Downton Draggy.
Season 2
- This season's Rusical challenge was Rats: The Rusical. The show also featured a song that was an extended Take That! against the then-recent film adaption. The costumes for the "Rat Pack" feature conspicuous green gloves intended for Chroma Key, a reference to the notorious Blooper in the film's first theatrical cut in which Judi Dench's human hands could be seen in several shots without the CGI fur applied over it.
- The season's improvisation challenge is "Morning Glory", a parody of morning talk shows in the vein of Good Morning Britain and Lorraine.
- The singing challenge is the RuRuvision Song Contest.
- In response to Bimini's amoeba look for the "Pre-her-storic" runway, Ru states "You know Michelle, we're living in a bacterial world and she is a bacterial girl."
- The acting challenge BeastEnders.
Season 3
- Veronica Green enters the workroom with her face and body painted green. She then asks “What's that sickening feeling? Oh, yeah. It's deja Ru!”
- The episode 2 challenge is to lead workout routines for the Drag fitness program Dragoton, a reference to exercise bike brand Peloton
- In this episode, Kitty states “The edgiest thing I’ve done is black lipstick, and I literally look like I work in a pier in Barry Island.”
- At the beginning of Ru’s walkthrough for the Snatch Game episode, he asks the group “How’s your Blankety Blank?”, the name for the UK version of Match Game.
- The acting challenge Bra Wars: The Fempire Claps Back
- Kitty’s character was named Brabarella. And Vanity’s was Baby Yolo.
- In this sketch, Ella commands an army of Braleks.
Season 4
- Episode 2's challenge has the queens forming musical groups to perform at the Yass-tonbury Festival.
- For the episode 3 mini challenge, Ru hosts an award show called the Naff-ta's.
- The award statuettes are a reference to a bootleg RuPaul Christmas ornament that went viral during Christmas 2021.
- Episode 4's challenge is an improv challenge where the queens appear on a talk show called Catty Man, a reference to Alan Carr's talk show Chatty Man.
- Episode 5 features the musical Lairy Poppins.
- Episode 8 has the queens acting in The Squirrel Games.
