The white and pink birds Matilda and Stella in Angry Birds. Matilda was there from the beginning but had an Ambiguous Gender early on. Stella was introduced in Angry Birds Seasons. Matilda serves as the Team Mom, while Stella is a Girly Girl.
In Arc Style: Baseball!! 3D, Team Albatross has two girls (Rivertorrent and Blackwell) and 10 guys.
Blue Dragon has Zola and Kluke to Shu, Jiro, and Marumaro.
The protagonists of Chrono Trigger are an aversion, with three females (Marle, Lucca, and Ayla) to two males (Chrono and Frog) and one robot (Robo), though the robot is referred to by male pronouns. The optional party member, Magus, is male and thus tips the count to the guys' favor.
Cookie Run: Kingdom has Strawberry Cookie and Chili Pepper Cookie as the two female Cookies in GingerBrave's main group. Also, the five legendary cookies that can be unlocked include Moonlight Cookie and Sea Fairy Cookie. Inverted with The Five, a group of Cookies who served as kings and queens of the kingdom. Pure Vanilla Cookie and Dark Cacao Cookie are the only two males in the group.
If you want to get technical about it, the protagonists kind of count as well, since they're two girls who occasionally gain a temporary third companion who's always male (variously Yuta, Shirokuma, Nagisa, and Haiji); although that's not really the most common set-up for this trope, and in fact might be something of an inversion.
The first couple of games in The Dark Pictures Anthology (which always features five playable protagonists) followed this trope, although House of Ashes and The Devil in Me then switched it up with a 4:1 and 2:3 male:female ratio, respectively.
House of Ashes does actually feature this trope in a wider sense, as Rachel and Clarice are the only women on the US army team as a whole — in fact, save an unnamed woman in the prologue, they're the only two female characters with speaking lines in the game — but Clarice isn't playable while Rachel is.
Dragon Age usually averts the trope, but there are some exceptions:
The Awakening expansion for Dragon Age: Origins has this. Unless the player character is female, three out of the six characters are male. One is a spirit and technically lacks a gender, although it's often referred to as a he, and Sigrun and Velanna round out the team.
Seen in Dragon Age: Inquisition with the members of the Bull's Chargers, the Iron Bull's mercenary company; of the group, only two - Dalish and Skinner - are female.
Dragon Quest VI has two female party members, Milly and Ashlynn, out of a group of six.
Dragon Quest VII has Maribel and Aira/Aishe out of a group of 6 characters. Maribel is a rebellious mayor's daughter and Aira/Aishe is a warrior princess.
The 3DS version of the game added the NPCs Morrie, the master of the Monster Arena, and Red, a female thief to the playable cast, bringing the number of playable characters up to 6, with 4 male and 2 female characters.
Ana and Pippi in Earthbound Beginnings, even though Pippi was only a temporary party member which you have to escort home after finding her a casket in the graveyard with zombies everywhere.
Final Fantasy II does have only Maria and Leila as playable female characters. Maria is The Heart of a group of three that sticks together for most of the game. Leila is a badass Pirate Girl.
While Final Fantasy IV has three female party members total, one of them, Porom, is only in your party briefly. The team you have for most of the late game - Cecil, Kain, Edge, Rydia and Rosa - has two females (Rydia and Rosa) and three males.
On that note, Final Fantasy V actually turns this on its head - your initial party looks like three males and one female, but later on, Faris is revealed to be a Sweet Polly Oliver, making it turn out to have been balanced. Then Galuf dies and is replaced by his granddaughter, and it becomes a four-person party with only one guy.
It's worth noting that in Final Fantasy X-2, your party consists of three girls... and nobody else.
In Final Fantasy XII out of the thirteen Espers, only two are female. In-universe they're a team of guardians who rebelled against the Gods.
Special mention as well to Final Fantasy VII. While the party does include 3 playable female characters, one of them is completely optional and another suffers from Plotline Death partway through. So The three don't have the full game to fight alongside each other, with only 2 left halfway through the game- playing this trope straight.
While the original Moon Angel Wing in Galaxy Angel was entirely composed of female pilots, the Rune Angel Wing in the Galaxy Angel II trilogy inverts the trope having two male members, Kazuya Shiranami and Roselle Mateus, though Roselle joins from the second game onwards.
In Genba No Kizuna, out of the four playable characters, two of them - police officer Himatsu Mizu and forensic investigator Rei Shirayuki - are female.
Inverted in Granblue Fantasy, with both the Six Dragons and the Ennead raid bosses (or Diviners, as "Heart of the Sun" refers to them). Both groups have only two male members — Wilnas and Lu Woh for the Dragons, Atum and Bennu for the Diviners — with the rest being female.
In Gravity Circuit, only two of the Rebel Circuits, Trace (the Patch Circuit) and Medley (the Wave Circuit) are female, with the other six being male.
Noble Team is this in Halo: Reach if Noble Six is female; otherwise, Kat is the only female on the team.
In The Great Ace Attorney, most of the juries have four men and two women. The only exception is the third chapter of the first game, the first to feature a jury, in which there are three female jurors.
In Kingdom Hearts III, Kairi and Aqua are this for the Guardians of Light, while Larxene and Xion are this for the Seekers of Darkness.
Although there's a total of three playable female characters, due to one of them being kidnapped relatively early in the game, the vast majority of Lunar: The Silver Star is played with two female party members, Jessica and Mia. Interestingly, the sequelinverts this, with the only male party members being Hiro and Ronfar for the majority of the game, although another male joins late in the game.
Inverted with the playable heroes from Hyrule Warriors. Out of the 10 playable heroes in the base game, only two are guys.
The Sages in Zelda games are mostly made up of women (if not all women). This may simply due to Women Are Wiser. Only A Link to the Past's backstory implies that all the Sages were male.
The Knight Academy in Skyward Sword only has two female students (Zelda and Karane), while the other six are male.
Metal Gear Solid is a strange case. Officially, Naomi Hunter and Mei Ling are the only named female members of Solid Snake's support team, with Nastasha Romanenko being optional (though canonically she joins). Meryl Silverburgh becomes one for a while up until a certain point, in which point it depends on the player's decision to a certain scene whether she finishes the game alive or dead (canonically it's the former).
Metal Gear Solid 2: Sons of Liberty plays with his a lot. For most of the game, Raiden's girlfriend Rosemary is the sole female member of his team. Then Emma Emmerich becomes one for a brief period until she's killed-off. However, it is then revealed that Raiden's Mysterious Protector "Mr. X" is actually the Good All Along Olga Gurlukovitch.
Metal Gear Solid 3: Snake Eater: The prologue has Para-Medic and The Boss as part of Naked Snake's support team, but The Boss pulls a Face–Heel Turn and joins the enemy faction. For the actual game, Para-Medic returns as part of Snake's support crew with EVA now joining him as his insider.
Mighty No. 2 Cryosphere and Mighty No. 3 Dynatron from Mighty No. 9. It was originally proposed that Dynatron and Mighty No. 8 Countershade would be the females of the Mighty Number team, but the lead character designer thought Countershade's radar and camouflage theme was more appropriate for a male character, so he made Cryosphere a girl instead, reasoning that Cryosphere's water and ice theme was a better fit for the proposed gender.
In the Game Boy Advance game Monster Force, a mummy named Mina and a vampire named Drew were the games' two unlockable characters as well as the only two female playable characters.
In Need for Speed 2015, the main racing crew consists of Amy and Robyn (female), Spike and Manu (male), and the Player Character (who is implied to also be male).
In Night Trap, we have the Special (or "Sega" in the Sega CD and 32X versions) Control Attack Team whose members (besides a Token Black Teammate named Collins) consist of the only two women in the group: Kelly Medd (the mature young woman posing as one of the teenage slumber party guests at the Martins) and Swanson.
Inverted in Odin Sphere. Out of the five playable characters, only Cornelius and Oswald are male.
Parasite Eve: Aya Brea and Cathy are the only female cops shown in the NYPD 17th Precinct, not counting Sheeva the police dog who dies anyway.
This is generally averted across the Persona series, with overall parties consisting of between eight to nine members with an even gender ratio about 85% of the time. Such is not the case in the Persona 2 duology.
For Innocent Sin, the initial party of five has two males (Tatsuya and Eikichi) and three females (Lisa, Maya, and Yukino). However, the final party swaps Yukino for Jun, and she lacks the Character Development and ties to the greater Myth Arc that Jun and the rest of the cast have. To add to this, Tatsuya is the protagonist of this part of the duology.
Eternal Punishment has Maya taking the protagonist role, but the final party plays this straight once again (Maya, Ulala, Baofu, Katsuya, and Tatsuya). You get to pick your initial fifth party member prior to Tatsuya's recruitment this time, meaning you can play this straight through the entire game by picking Kei or averting it by picking Eriko.
Plants vs. Zombies: Heroes: This is played straight with the Zombie side Heroes, who have Immorticia and Neptuna as the only 2 females out of 11 heroes. Averted with the Plant Heroes, who have 5 females out of 11 heroes.
In Psychonauts 2, Cassie O'pia and Lucrecia Mux are the only two female members of the Psychic Seven (later Six) before the latter left.
In Puyo Puyo Tetris, the S.S. Tetra's otherwise all-male crew has two female members, Ess and Elle.
Puzzle Quest: Challenge of the Warlords has this. Only two of the potential team members are female (unless the player character is as well) - Princess Serephine and Elistara.
A literal example with Jill Valentine and Rebecca Chambers in the first Resident Evil, although they technically work for different divisions of S.T.A.R.S.
Seven Knights has Eileene and Rachel as the two females in the titular group. Although neither of them is The Heart, as both of them portray different styles of the Lady of War trope. Eileene wields a spear and fights with brute strength, whole Rachel wields a Royal Rapier and fights elegantly.
Gameplay-wise, played straight by Skies of Arcadia: as there is indeed a 4 guys: 2 girls ratio. Inverted by its story, however, as only one of those guys is a mandatory party member. The other mandatory party members? The two girls. As such, they have much more screen time than the other three guys.
King and Yuri Sakazaki in the first two Art of Fighting games (although Yuri was only an NPC in the first). The third game had three female fighters (Kasumi, Lenny, and Sinclair).
Mai Shiranui and Blue Mary from Fatal Fury 3 and onward (before the addition of Li Xiang-Fei in Real Bout 2 and Tsugumi Sendo in Wild Ambition).
From the Sonic Advance Trilogy, Amy and Cream are the two girls of the 5 members of the heroes, of which the rest are Sonic, Tails, and Knuckles.
Sonic Mania: Of the Hard Boiled Heavies, Heavy Magician and Heavy Rider are both referred to as female within the game's manual.
Sonic Superstars: Amy and Trip, after her Heel–Face Turn, serve as the two girls to the team, wherein the rest consists of Sonic, Tails and Knuckles.
South Park: The Fractured but Whole originally followed The Smurfette Principle and had Call Girl AKA Wendy as the only female of the game's twelve possible party members, not counting the New Kid if the player chooses to make them female, but the From Dusk to Casa Bonita DLC adds Henrietta Biggle as a playable character not only in the DLC but also retroactively in the main story. True to the trope, the two are very different in terms of personality and abilities, with Call Girl being a perky heavy damage dealer with powers based around technology and Henrietta being an aloof support character with powers based around magic.
Super Paper Mario: Mimi and Nastasia are the only two female members of Count Bleck's minions.
Many of the Mario spinoffs released in the Gamecube era (as well as some late Nintendo 64 games like Mario Tennis and Mario Party 3) feature Peach and Daisy as the only female playable characters, though Birdo and Toadette would often appear and add additional girls to the roster. However, Birdo and Toadette's presence has never been quite as surefire as Peach and Daisy's. With Rosalina and Pauline having risen to prominence, this is no longer the case. Oddly enough however, the spin-off games may randomly lapse back into this trope despite the series having a decent amount of female characters to choose from, such as only Peach and Rosalina being playable in Mario Strikers: Battle League when the game first released. With Mario Party 5, this also applies to the hosts: carrying over from the original Paper Mario, out of the seven Star Spirits, only Misstar and Mamar are female.
Tales of Destiny gives us Rutee and Philia among the game's five Swordian Masters (the other three being guys). The remake inverts this among the non-magical heroes: giving us Chelsea, MaryandLilith against the guys Kongman and Johnny.
Tales of Rebirth has four guys and two girls among the main heroes. The villains have a similar setup: with Militsa and Zilva.
Tales of Graces has this ratio plus one - with a 4:3 guy:girl ratio in its Seven-Man Band. The original game had an even gender split.
In the original Tekken, Nina Williams and Michelle Chang were the only playable female characters in the starting roster.
The 2023 Toxic Crusaders game features Yvonne and Mrs. Junko as playable Crusaders for the first time, when the older licensed games only had the original Five-Man Band Crusaders at most.
Pai Chan and Sarah Bryant in the first two games of the Virtua Fighter series (plus the Virtua Fighter KidsGaiden Game).
Averted in Trials of Mana: there are six playable characters, divided evenly between male and female. Choosing three of them before the game starts (with the rest still appearing in guest roles as Non-Player Characters), you can have a party of all females, all males, or any combination of your choosing.
The original game had two female Reapers- Uzuki Yashiro, who's a low-ranking Harrier and Kariya's partner, and Mitsuki Konishi, assistant to the Conductor and Game Master for the third week. Coco, a third female, was added in later versions of the game.
NEO: The World Ends with You, there are two female Reapers in the Shinjuku Reapers- Shoka Sakurane, who monitors players, and Ayano Kamachi, a higher-ranking female reaper who's an older sister figure to Shoka. It's subverted when it turns out that Tsugumi Matsunae, the sole female of the Ruinbringers, is also a Reaper.
Neo also has two female party members- Nagi Usui, who joins early on in the game, and the aforementioned Shoka, who joins later on.
