Inverted with Angelina Ballerina in both the 2002 and 2009 series. William and Henry (Angelina's younger cousin) are the only two boys in Angelina's predominantly female ballet class. The Next Steps has Marco and A.Z. as the only regular boys in the cast.
Animal Mechanicals: Mouse and Unicorn are the only girls on the team, with the boys being Rex, Komodo and Sasquatch.
Avatar: The Last Airbender plays with this trope, the first season runs very much on The Smurfette Principle with Katara as the only female out of three, the second season then adds Toph which evens it out exactly, the final season Suki is the 11th-Hour Ranger to the team... except Zuko already made his Heel–Face Turn and joined... keeping the team even.
Classic Disney Shorts had Minnie Mouse and Clarabelle Cow, with Clara Cluck and Daisy Duck being added later. Even then; the "Sensational Six" has Minnie and Daisy act as the two girls to the ensemble.
Also, there are two female members of The Delightful Children from Down the Lane.
Caprice and Cassandra in seasons 3 and 4 of The Crumpets.
DuckTales (1987) and its 2017 reboot feature Webby Vanderquack and her grandmother Ms. Beakley. The reboot would then introduce another female member to its core cast using DellaDuck. In "GlomTales", Glomgold's Legion of Doom includes Ma Beagle and Magica De Spell.
Not counting the Kanker Sisters (who aren't part of the cul-de-sac), Ed, Edd n Eddy has Nazz and Sarah.
Family Guy has Meg and Lois in the Griffin family.
With the 2015 introduction of Ellie Phillips, the fire service has two female members, Penny and Ellie, alongside four male members, Sam, Elvis, Station Officer Steele, and Arnold. The other divisions of the rescue services typically have a duo of a man and woman; Tom and Krystyna for mountain rescues, Ben and Jodie for ocean rescues, and Malcolm and Rose for search and rescue.
When the Flood family joined the cast in 2003, Sarah and Mandy became the two core girls of the child cast, alongside boys James, Norman, and later Derek. This is averted in Seasons 8 and 9 with the introduction of Lily and Hannah.
Tomboy and Girly Girl Wendy and Mabel in Gravity Falls. In the same show, there's also Wendy's friend group, where she and Tambry are the only two girls outnumbered by four guys (Robbie, Nate, Lee, and Thompson).
In Handy Manny, Manny has two female tools, Dusty the hand saw and Squeeze the pliers.
Justice League: Hawkgirl and Wonder Woman, two of seven. While their appearances might suggest a Tomboy and Girly Girl dynamic, the actual contrast was in their experience (with Wonder Woman as the Naïve Newcomer and Hawkgirl as a bit of a cynic) and in their attitudes towards men, with Wonder Woman having a touch of Women Are Wiser at first. Notably, neither was The Heart, as that role was filled by the Flash.
Wonder Woman:You men! Unless you do it on your own it doesn't count! Hawkgirl: It's not just the men, you stuck-up...
Inverted in the second season of Miraculous Ladybug. Though they rarely all work together in the same episode, Adrien/Cat Noir and Nino/Carapace are the only males carrying Miraculous. The third season introduces more heroes, and to date, it's roughly equal between the genders.
Numberjacks: Shape Japer and Spooky Spoon are the only two female meanies, while the other three meanies, The Puzzler, Numbertaker, and Problem Blob, are males.
In the Oh Yeah! Cartoons short "Planet Kate", Cleo and Juno are the only female members of the quintet of alien dogs Kate Moon is acquainted with.
As of Season 2, PAW Patrol has two female pups, Skye and Everest.
Phineas and Ferb: Some episodes (most notably "Summer Belongs to You" and "Last Day of Summer") have Candace joining the main group of children (Phineas, Ferb, Isabella, Baljeet, and Buford).
Recess gave the Five-Man Band a super-duper dorky girl Gretchen Grundler and One of the Boys Ashley Spinelli. Also the school's "Bad kids" had Sue Bob Murphy and Kurst the Worst.
Daphne and Velma of Scooby-Doo were an early example of being the only two female members of a group otherwise composed of males.
Seven Little Monsters has One and Six as the only two female members of the titular monsters.
In Season 2 of Shirt Tales, there's Pammy Panda and Kip Kangaroo.
Inverted with The Simpsons family itself; there are two boys (Homer and Bart) to the family (three female members, two male members).
Skysurfer Strike Force gives us a villainous example with Lazerette and Cerina of the Bioborgs.
The Smurfs (1981) themselves had this later on with the introduction of Sassette.
Amy Rose and Sticks the Badger in Sonic Boom. Interestingly, the video game versions played The Smurfette Principle trope until 2015, when the then-announced Fire & Ice revealed that these two were playable together.
Sonic Prime has Amy and Rouge the Bat as the two girls on Sonic's team.
Space Racers has Robyn and Starling as two of the five young cadets at Stardust Bay Space Academy.
SpongeBob SquarePants: When creator Stephen Hillenburg first pitched the show, the main cast◊ had two female characters (Pearl and Sandy) to five males (the title character, Patrick, Squidward, Mr. Krabs, and Plankton). After the series had been in development for a while, the cast expanded to include two more females (Karen and Mrs. Puff) as well as another male (Gary).
Among the rough group of main characters, Tomboy and Girly Girl Tam and Torra. Later, female-programmed droid CB-23 joins the group as male droid BB-8's replacement, averting the trope.
Among the five-person Ace Squadron, Torra and Freya.
In early versions of Super Friends, the team had Wonder Woman and Wendy, or Wonder Woman and Jayna. According to the other Wiki, the only additional female superheroes who ever made repeated appearances on the show were Hawkgirl and Rima the Jungle Girl, but neither appeared in a large number of episodes. One version of the show, Challenge of the Super Friends, featured a team in its first segment where the only female superhero was Wonder Woman. Jayna appeared as a regular in the second segment of that show. The Challenge of the Super Friends episodes featured a Legion of Doom with two female villains, Cheetah and Giganta.
Super Why! has Red (Wonder Red) and Princess Pea (Princess Presto) on the main team. Notable as the main team has four characters in it, making this an even split between the male members, Pig (Alpha Pig) and Whyatt (Super Why) and the female members mentioned. Note that this is a Five-Man Band — it's just that the fifth member is supposed to be the viewer, who of course could be of any gender. Later on, they added a male character named Puppy (Woofster), making this a more typical example of this trope (three males, two females).
The Sushi Pack has three males (Tako, Ikura, and Wasabi) and two females (Maguro and Kani).
Tangled: The Series had Rapunzel and Cassandra as the only two prominent females in the first two seasons, before Cassandra made a Face–Heel Turn in the third.
The team starts with four guys, then adds Miss Martian and Artemis Crock. This becomes the status quo for the bulk of the first season. This trope was so enforced in the minds of its demographic that rumors that another female team member (either Wonder Girl or Secret) would be joining the cast launched much speculation that either Artemis or Miss Martian would be outed as The Mole, die, or otherwise leave the team.
Averted, however, with the inclusion of Zatanna as a long-term member without any other character being removed from the team. As for the series' other big team, the Justice League, although there are three female members on the team, which technically means it doesn't count, the full roster is sixteen members so the essential truth of this trope is still in play. Interestingly, Wonder Woman seems to be actively working against this, as one of her stated criteria for an ideal new member to the League is that they be a woman to help shift the gender ratio.
By the end of season one, Zatanna is a full-time member and Rocket has joined up, so that the gender ratio is even with four boys and four girls. As of season two, the ratio for the team stands at five males and four females.
By Phantoms, this trope has perhaps become inverted, as the Team is now mostly comprised of female heroes, with the only two known male members being Nightwing and Arsenal.
This continuity's version of the Doom Patrol has Elasti-Girl and Negative Woman as its only two female members.
◊ had two female characters (Pearl and Sandy) to five males (the title character, Patrick, Squidward, Mr. Krabs, and Plankton). After the series had been in development for a while, the cast expanded to include two more females (Karen and Mrs. Puff) as well as another male (Gary).
