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Anime & Manga
- One-Punch Man: Saitama planned to end his initial confrontation with Speed-of-Sound Sonic by threatening him with a punch to the crotch (which would have been much more than painful) that he'd stop just short of connection. Sonic could not stop himself fast enough, so his groin crashed into Saitama's fist. Sonic's attempt to bow out from the fighting gracefully is undercut by his shaking knees and increased-pitch voice.
- An animated commercial for Duluth Trading Company's Ballroom jeans shows a large man squatting in a competitor's brand, and singing soprano while doing so.
- A Lee jeans commercial from the early 90s had a singing man who recently emerged from the shower go from a deep bass to a high soprano the minute he zipped up his tight-fitting jeans.
- Referenced by Dona Hermine in De cape et de crocs.
Dona Hermine: Come any closer and you'll be able to sing for the pope!
- In Animal Man, the Bakers' neighbour, Morris Weidemeir, fires a warning shot at Ellen's would-be rapist and tells him, "One more step, sonny, you're singing soprano."
- In a MAD gag, a man tried a helium vending machine for a dollar. His friend wanted to try it but only had a quarter, which was just enough for the "helium substitute" machine next to it, which sent a mechanical foot straight into his crotch.
- Hinted at in the Empath: The Luckiest Smurf novel in regards to Hefty and "the following action that would have left him hitting the high notes for the rest of his life".
- Plan 7 of 9 from Outer Space. After an Amazon Brigade of female cyborgs threaten to have our heroes castrated, Buster Screams Like a Little Girl causing Captain Proton to think they've started already.
Films — Animation
- In The Hunchback of Notre Dame, four guards let out a high-pitched squeal (but not helium sounding) after receiving a simultaneous crotch kick from a guy in stilts.
Films — Live-Action
- In The Ice Pirates, the eunuchs speak in falsetto after being castrated during their conveyor-belt ride.
- Happens to Topper's unfortunate opponent in the opening scene of Hot Shots! Part Deux.
- This happens to Jernigan in Home Alone 3 after Alice hits him with a crowbar when attempting to hit Doris the rat, who had crawled into his pants.
- The Inspector Gadget movie has Gadget grab a guru's testicles when told to "reach out and grab the balls" (meaning the marbles on a pedestal). The guru's reduced to squealing "TURN IT OFF! TURN IT OFF!" in a very high voice.
- Happens in The Three Stooges 2012 movie when Larry gets a lobster down his pants.
- In The Pirate Movie, a comedy 1980s version of The Pirates of Penzance, the enemy Chinese pirate says/sings "Spare me!" and the Pirate King says, "Ooh, an Irish Tenor, no less! We could use an Irish Tenor, lads!" He accidentally stabs the man in the crotch and the guy goes "yeeOOOWWW" in a very high-pitched voice. "Soprano! Even better."
- In A Christmas Story, the Old Man gets a bowling ball dropped in his lap for Christmas, and his voice goes at least a little bit higher.
Well, it's a blue ball!
- In History of the World Part I, to a Roman soldier after Comicus (Mel Brooks) kicked him there.
- Again in Robin Hood: Men in Tights when Blinkin offers a handshake to Achoo, and inadvertently hits him square in the crotch.
Blinkin: How're you doing?
Achoo: (high pitched) I've been better!
- In Hook, Peter's voice goes briefly high-pitched after he's hit with a (thankfully-blunt) arrow in the groin.
- In the lost ending of Freaks, Hercules the strongman would have been punished by the "freaks" by castration. He would have then become a castrato singer, and now a freak himself.
- In the movie Summer Rental, John Candy's character gets socked in the groin and he starts singing the first line of "The Star-Spangled Banner", which has a rising high pitch.
- In the movie Howard the Duck, Howard barely survives a butcher knife landing near his groin and he responds in a soprano.
- In The Running Man, Ben Richards shoves the blade of Buzzsaw's titular weapon into his groin area, causing him to emit a scream that went higher in pitch.
- Francis lets out a high-pitched scream in The Goonies after he slips a on log over a stream of water and lands on it groin-first.
- The Toxic Avenger
- There is a dutch joke that, translated, goes like this: "Two men are swimming in the ocean, when one of them glimpses something he recognizes beneath the surface. Quickly swimming to his mate, he shouts, "We have to leave the water immediately! I just saw a SHARK!" If it's not clear, the speaker raises their own voice to soprano level the moment they say "SHARK!". This provides context of what the shark has just taken a great big chomp out of without stating it directly.
- In Cold Copper Tears, Garrett refers to members of a nihilistic cult as "sopranos" after finding out that they're all ritually castrated.
- Happens several times in The Dresden Files:
- In Dead Beat, Will returns Harry's pistol to him, having carried it tucked into his pants. Harry remarks that this is a good way to invoke this trope on oneself.
- In the short story "Heorot", Harry hits the grendelkin in the fire extinguisher while they're fighting. Although his opponent's post-injury voice doesn't sound particularly high-pitched by human standards, the huge creature's bottomless-well-deep speech does rise by a couple of octaves, to something more typical of humans than of giants.
- Harry himself gets subjected to a Groin Attack, and subsequent involuntary Mickey Mouse impression, in Cold Days.
- The Discworld Fools' Guild Diary mentions an incident in which King Harold the Rampant neglected to comply with his jester's suggestion that he adjust his chain mail. This negligence, together with the very good aim of a Pseudopolitan archer, is why the Battle of the Field of Cloth and Ants ended on such a remarkably-high note.
- In Sourcery, the slaver captain asks Rincewind if he can sing before revealing his intent to sell him as a eunuch.
- In The California Voodoo Game, one character glares at another with such hatred that, if looks could cut, the other would've been eligible for First Soprano in the Vienna Boys Choir.
- The Gorillaz autobiography Rise of the Ogre discusses the filming of Clint Eastwood, which had a zombie grabbing Murdoc in the balls. According to Murdoc, it really hurt and he was "singing falsetto for weeks".
- Mary Roach, in her non-fiction treatise on science and sex Bonk, cites the case of an obsessively devout man who, taking the Bible's Matthew 19:12 all too literally, removed and destroyed his own testes. She speculates that he went on to achieve great success in the church choir.
- Journey to Chaos: After delivering a Groin Attack to his enemy, the girly looking Getis, Eric notes that his following Who Dares? was extra girly and not in the least threatening.
Live Action TV
- In an episode of Red Dwarf, Rimmer says he believes that in a past life he was Alexander the Great's... chief eunuch. Lister responds, "No wonder you're such a good singer."
- In All in the Family, Mike spoke in falsetto after having a vasectomy. (He was only kidding.)
- Referenced in Band of Brothers when one of the troops sings a line from Oklahoma! in tenor, and another soldier teases him, asking if he was sitting on his bayonet.
- Referenced in a holiday episode of Fear Factor — the host described a plate of ten reindeer testicles as "five reindeer singing soprano".
- Done on Cheers when Rebecca accidentally hits Sam in the crotch with a coatrack, excited about the prospect of making a baby with him. Later, as he sings to her seductively while they dance, she comments that she didn't know he could reach a note that high. "I couldn't before the coatrack," he admits.
- A rare female version occurred on The Big Bang Theory. Amy spoke in a higher register for a while after receiving her first bikini wax.
- Hawkeye from M*A*S*H complained that he'd become a soprano when he was suffering from stress-related impotency.
- A blooper that was left in the final cut of All That, Kenan, during one of the Pierre Escargot sketches accidentally dropped a prop on his groin. He delivered his next lines in a high-pitched voice.
- In The Red Green Show, Red mentions this happening to Buster Hatfield during a hockey game after taking a slapshot from Moose Thompson.
- In the Stargate SG-1 episode "Seth" Daniel had previously informed the team that past cults of Seth sometimes turned their men into eunuchs. When Seth gasses them later:
Jack: So help me, if I wake up and I'm singin' soprano... (passes out; fade to black)
- A variant occurs in the So Little Time episode "True Lies", where Jake speaks in a hilariously high-pitched voice after having frozen food shoved down his pants.
- The Goodies: "Saturday Night Grease" has Tim talking in really high pitched voice that drops to his normal register as he unzips his ridiculously tight trousers.
- Black Books, "The Blackout". A Running Gag has Manny, who's drank so much coffee and watched so much The Sweeney that he's permanently stuck in hyped-up cop mode, trying to sit on a chair backwards like a suave cop. It doesn't work because he keeps "sitting on meself". The very last time he does it, to close out the episode, he says in a helium-induced pitch:
Manny: "That was a particularly bad one."
- A non-painful variant from Doctor Who. When Cassandra - in Rose's body - kisses the Doctor completely out of the blue, the latter freezes for a couple seconds during Post-Kiss Catatonia, before muttering how he's "still got it" in a very squeaky voice.
- In the City Limits episode of Mystery Science Theater 3000, while viewing a brawl between a would-be Clippers gang member and some thugs, one of the bots quips about a Groin Attack:
Look, now he's a Vienna Boys Clipper!
- The Paul and Storm song "Why, Baby, Why?" is a falsetto doo-wop in which it turns out that the woman he's singing to has just done this to him.
- John Barrowman while singing "Anything You Can Do", as seen here.
- Richard Harris's version of "MacArthur Park" does this on the last note, which is sung by a female backup vocalist.
- Woody Allen once claimed that he was punched by a criminal:
"He snapped his knee up, quickly, and I refused to give him the satisfaction of doubling over, but I did one of the greatest imitations of Lily Pons, you've ever heard. I hit an M over high C..."
- In one of the Grimtooth's Traps books, this trope is cited in the name of the so-called "Soprano Chest", which has a heavy lid and a board sticking out at the bottom front, designed such that when the chest it opened, the whole thing tips over backwards, sending the board up between a character's legs.
- In the second Bottom Live tour, Richie (Rik Mayall) and Eddie (Ade Edmonson) are fighting, when Rik accidentally punches Ade in the nads. Out of character, Rik apologises to Ade, who speaks a couple octaves higher to complete the effect.
- Subverted in Wolfgang Amadeus Mozart's opera Escape from the Seraglio, in which a eunuch (presumably castrated after puberty) has a deep bass voice.
- In TimeSplitters: Future Perfect, Jo-Beth Casey briefly mistakes Cortez for a zombie and kicks him in the crotch. His next line is a high-pitched squeak.
- Schlock Mercenary: Dr. Bunnigus threatens one of the grunts with this after word gets out that she used to be an exotic dancer.
Nick: I saw the news. . . Izzit true you used to be an exotic dancer?
Bunni: (sigh) Yes, It's true.
Bunni: How would you like to be able to count to ten as a soprano, Corporal?
- The Nostalgia Critic does this a lot, and ends up sounding like he's on helium. Notable cases include his reviews on Demolition Man and The Wicker Man (2006).
- Dragon Ball Z Abridged: When Cooler kicks Vegeta in the dick, Vegeta's scream of abject pain is slowed down while "Ave Maria" plays over. Later in Super Android 13, Goku displays this trope by asking "Why did he punch me in the dick?" sounding like Gohan. When you have your testicles pop from a single punch you'll sound pretty high.
- RWBY: In the "Yellow" trailer that introduced Yang, she interrogates Junior by grabbing his crotch and squeezing it, resulting in his voice sounding like a little girl until she lets go.
- Cobra Kai: "Different, But Same" has a rare girl-on-girl variant via the Melvin, when Aisha reaches into Yasmine's shorts and yanks on her thong. "NO MERCY, BITCH!!!"
- Batman: The Animated Series: At the time, a Groin Attack could be included in the show with careful positioning. To indicate to the viewer that it actually had happened, note the Joker's noticeably girlish higher voice after.
- Stan from American Dad! went through this after stepping on a rake, hitting him in the groin causing his testicles to retract.
- In the intro to the Kim Possible Movie: So the Drama, Ron faces a Sumo Ninja, who speaks with a deep voice. After Kim beats him, Ron gives him a wedgie. At the end of the movie, the Sumo Ninja comes back, looking for vengeance... only now, he speaks with a high, squeaky, girly voice.
- Star Wars Rebels: Lando Calrissian has this after being on the receiving end of a Groin Attack (delivered via fist) from Hera Syndulla, thanks to his using her as bait in a Batman Gambit, resulting in him sounding like he's on helium.
- In the 2 Stupid Dogs episode "Las Pelotas!", when Big Dog gets hit in the crotch by tennis balls, he replies to Little Dog's statement of "Balls!" by saying "Yeah, I know", in a much higher voice than usual.