Here's a list of characters and related tropes for the series Wandering Son.
Voiced by: Kousuke Hatakeyama
Attractive Bent-Gender: When she went to her elementary school reunion dressed in mostly masculine clothes with a short haircut she got a lot of attention from the girls. One of the girls from her elementary class even had a crush on her.
Meaningful Echo: Throughout the story, whenever she talks or confesses about her transgenderism, she always says, "I want to be a girl." But in the final line of the manga - the title of her book - she instead simply says, "I am a girl."
Meaningful Rename: After finally deciding to transition at the end of the manga, she asks Yuki to give her a feminine name. She calls her "Lily".
Yamato Nadeshiko: Sorta. She's a soft-spoken, docile child who's good at baking and is quite feminine. She says what she feels and has spoken up for her friends at times. In later chapters she's begun to break more out of this trope but still fits.
Adaptation Dye-Job: Or actually, Story Progression Dye Job. Takatsuki's hair was dark brown in the first two volumes before becoming the black we know of today.
Played straight with his eyes. From black to gold.
Attractive Bent-Gender: He looks very pretty during his runway walk during the cultural festival with Shuuichi. The judges for the modeling audition thought so too, and praised his good posture.
Berserk Button: Bullying Shuuichi. Also, his period and, to a lesser extent, other physical or social aspects of femininity coming from his biologically being female. Teasing him about it made some boys in his elementary school seriously beaten. He's generally only tough in situations he feels pressured in.
D-Cup Distress: He hates it when his breasts begin to grow. He even goes to buy a garment to flatten his chest so that he does not have to wear a bra.
Hot for Teacher: He seems a bit confused about whether it was a crush or not. He felt pressured by everyone else to have a crush on somebody, but other people thought he fell for a teacher in middle school.
Adaptation Dye-Job: Her eyes are black past-elementary but the anime still has her with brown eyes
Cannot Tell a Joke: She only tries once in the manga but her "joke" didn't come off as one and just created awkward silence.
Characterization Marches On: A Justified case. In early volumes her personality was very different, but then, again - it is a story partially about how adolescence and circumstances in it can form child's character.
Girlish Pigtails: Wore them in middle school, and oddly only while at school; she had her usual cut when in casual clothes.
Good Eyes, Evil Eyes: Her eye style changed early on. Originally she had the more wide-eyed expression everyone else does, though she eventually permanently changed to her sarcastic look. She essentially went from Tareme Eyes to Tsurime Eyes.
The Smart Girl: Perceptive and sometimes more self-confident than Shuuichi, she frequently supports and defends her from others' inadequate aggression, even cutting Maho's bullying of her sister and shutting her up once.
Transsexual: She's a Foil to Nitori, being much more self-anxious and nervous about presenting as a girl.
Uncanny Family Resemblance: With Shuuichi. She even lampshades it, saying after the audition that they could've said that're twins.
What the Hell, Hero?: Makoto gives Maho one of these, when witnessing her mistreatment of Shuuichi. Interestingly, having to deal with an assertive and self-confident girl so different from her sister, Maho cannot really tell him off.
Crossdresser: She seems to prefer female or androgynous styles in casual wear outside of school but she doesn't think twice about dressing in her male middle school uniform. She even wore it on the first day of school.