- Jerkass Woobie: Sylvester is a conniving predator in most his shorts, however he is always completely out of his foes' league and earns more than a few solid beatings in nearly every appearance he has. Not to mention he was often at the recieving end of abuse from unprovoked adversaries as well. His neuroses and small number of victories even compared to other Looney Tunes Butt Monkeys makes him arguably the most pitiful villain in the series after Wile E Coyote.
- My Real Daddy: Friz Freleng for Tweety. He was the primary director for most Sylvester shorts as well, though other directors did make notable use of him.
- Nightmare Fuel: In "Greedy For Tweety", Granny gives Sylvester sleeping pills, rendering him too tired to move. His eye are blood-shot as he snaps his eyes open after they slowly close over and over... while Hector the Bull dog with his broken leg, inches closer every time Sylvester closes his eyes... And there's nothing Sylvester can do but wait for the inevitable.
- Squick: The scene in "Museum Scream" where Sylvester goes through a digestive tract display. Tweety's line after Sylvester emerges ("Are you okay, puddy tat? You wook pooped!") doesn't help.
- Unintentionally Sympathetic: Sylvester falls into this a lot. It's especially hard not to feel any sympathy for him in Canned Feud, where he doesn't even do anything to provoke the mouse who gleefully torments him throughout the short and wants to make him starve. There's also "Museum Scream", where, yes, he is trying to eat Tweety, but much of the abuse that he takes in the short is needlessly harsh (getting swallowed whole by a snake and attacked by a wasp, having his head bitten by a baby dinosaur, being sliced to pieces by a buzzsaw, Tweety making him go through a digestive tract display, ect.).
- Viewer Gender Confusion: Tweety is one of the most common examples of this in western cartoons. His high pitched voice is supposed to be boyish and childish instead of girly. He has eyelashes and a soft color palette, but is a boy nevertheless. It doesn't help that a lot of merchandise with Tweety on it are aimed at girls. At least one gag in Sylvester And Tweety Mysteries lampshades this by having Tweety fly into a birdhouse bathroom with a male sign on it, then poke his head out to the audience and say "See, folks? I am a boy."
YMMV / Sylvester the Cat and Tweety Bird