This advert for the German dairy company Muller starring Yogi Bear and KITT has fruit being flung at humans and turning them into the characters from the books.
ThisPublic Service Announcement entitled "Book People Unite" features Mr. Tickle, Mr. Happy, Mr. Tall and Little Miss Sunshine amongst other famous children's book characters.
The blue ball in this Biotene ad resembles Mr. Forgetful.
At 0:24 in this video, there is a commercial for Intercity featuring Mr. Clever, Mr. Busy and Mr. Wrong.
Chapter 23 of Twinkling in the Dark, which is about Joker suffering from congestion as a result of an illness, is titled "Mr. Nosey", after the snoopy Mr. Man.
An animated sketch on Dead Ringers has Mr. Happy walking to the wrong side of Mr. Men Land and meets Little Miss Chav in which she angrily told him to go away after he asked her for help to find his way home, Mr. Happy Slapper, Little Miss Asbo, who in which beats Mr. Happy up and then records it with their video camera and Mr. Crackdealer (who has the same appearance as Mr. Messy). And at the end of the sketch, Mr. Happy is shot in half by Mr. Drive-By-Shooter.
In The Golden Girls episode "Valentine's Day", a plush toy of Mr. Tickle can be seen on a shelf during the famous "Condoms, Rose!" scene.
In the Teletubbies episode "The Beach", a girl is seen wearing a Little Miss Sunshine shirt. This segment was featured in the home video release "Here Come the Teletubbies".
The SCP Foundation has the Little Misters, who are based on the Mr. Men and Little Misses to the point that some of the Little Misters share the same name as existing Mr. Men.note This includes SCP-909/Mr. Forgetful, SCP-6558/Mr. Nobody, SCP-2933/Mr. Scary, and SCP-6158/Mr. Brainy, though the last two are the names of Little Misses instead.