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: 8-Bit Theater Red Mage uses this as Black Mage is about to burn the Rat's Tail they need for the class change. He puts on a levitation spell so he can do the slo-mo jump (but applies too much and is left hanging for a while), and does the Big No just to be dramatic.
has several of these.
Darths & Droids has multiple examples playing with this trope.
El Goonish Shive Subverted in the third panel of this strip of , where a " Sluggy Freelance little no" gives the dramatic impact a Big No is supposed to have.
The Order of the Stick Vaarsuvius uses it in strip #504, titled " That's a Surprisingly Common Reaction". Lampshaded in the first panel of the next strip, by Genre Savvy Elan:
Elan: Hey, did someone in here just drop to their knees and scream, "Noooooo"? I got a feeling....
Durkon gets one of these in On the Origine of PCs after tasting what is allegedly the "finest beer brewed in all the human lands."
And the intermission comic, where... well just see for yourself.
V does it again after an as-of-yet unnamed dragon swears to kill V's kids.
Xykon, of all people, does it when his phylactery is nearly lost to the Snarl.
Xykon: No no no no no no NO! [...] Xykon: YES!
Tsukiko gets one once she realizes that her fate has been sealed.
has had its fair share of them:
Captain SNES: The Game Masta Dr. Wily does the traditional NOOOOOOOOOOOOOO! When he realises he's in a catch 22 as the VP of Nexus and doing anything to ruin Nexus would cause Blues to torture him, meaning he would have to go against his nature and save it from the stupidity that was President Haggar.
Alex Willams, replaces the Big No with the Big FUCK, and purposely extends it to buy time to think.
He did a simple Big No before. It lasted something like thirteen comics.
Girl Genius Plays... oddly when Gilgamesh Wulfenbach is led to believe that Agatha was killed by a rogue clank. He begins the wind-up to a Big No before cold logic takes over. However, in a flashback to the same scene, Gil is in the classic "Big No" pose. So it's unclear whether he did one or not.
Many Girl Genius characters get a Big No, of course. Gil demonstrates correct form here.
Scully gets one in when the Flirty Science Guy gets shot:
Monster of the Week
Scully: NOOOOO! Whose romantic advances will I politely rebuff now?
presents the rare Candi quixotically hopeful drunken ex-boyfriend version.
: When Vanessa is grabbed into a pit by a...thing.
Silent Hill: Promise : This very wiki is responsible in Today Nothing Happened this strip.
has it at the end of Bob the Angry Flower this strip.
Used pretty straight in Final Blasphemy here and later in the same issue.
In , Starslip Crisis Zillion can't even do this right.
In when Bob learns The Inexplicable Adventures of Bob! his roof is about to be destroyed a third time. It will happen many more times.
From , Multiplex sarcastically, from Kurt.
Candice didn't appreciate his creative marriage proposal.
Happens in when Noelle notices that Corner Alley 13 her café doesn't have any customers.
Right here in .
Elven Lacryment Dragon Mango spoofs this when Pumpkin's father returns after a four-year journey into faraway lands and gives Mango a rare treat, called a "sweet swirl stick", which Cherry eats in one bite.
Jason from has a fairly Something Positive unique one when realizing he's just turned down a 3-way with his wife and hot friend.
Rhea of 's Slightly Damned reaction upon learning that she cannot be reincarnated.
has the following conversation.
Powerpuff Girls Doujinshi Professor Utonium:
What's that you got there? Is that a... Wh—Th—P...P....
Buttercup: Parent-Teacher Conference... Professor Utonium: NNNNNOOOOOOOOOOOOOOOOOOOOOOOO!!!
A Nooooo! that bursts out of the panel in Jayden and Crusader.
Cale whips one out in a comic of .
Looking for Group has Dan and Mab's Furry Adventures this beauty when a young Abel learns that extra lessons include extra homework.
: Gabriel provides this Penny Arcade gem upon besting Tycho in a card game.
In , Kirby unleashes one when Brawl in the Family Meta Knight takes his revenge on him here.
Monica from had a nice one when Wapsi Square Phyx decided to "tag" Nudge.
The Princess of when she Rusty and Co. found the White Knight crushed.
parodied the original scene Loserz here.
Dork Tower audible five miles away
Chris in does this when he learns that a Starbucks barista only went out with him as a joke. Sonichu Apparently, this happened in real life.
Nith from discovers his home has been destroyed.
Whats Shakin is quite prone to this. For example:
Ménage à 3 Kiley here.
Zii's reaction when Di Di tries to cut her hair into a mullet.
Zii again here and here.
Kiley again here when she thinks Yuki is going to see her father in Japan when she's in a bad condition, and yet again here.
Princess Pi Alpha Bitch Sam yells this after getting sentenced to community service as punishment for blowing up Piscataway Stadium.
Princess Pi herself says this when the new princess, Gertie, makes her genie, Bottle Blonde, encase her own bottle in concrete, so that Pi could not make Bottle Blonde turn Piscataway back to normal.
has one after True Believers .
uses a stolen stamp to give Joe Quesadilla a Ret Gone In Ben Harkus gives us one of these Shifters here after
failing to catch Ferrah and letting her fall from a crashed plane that had been brought down by Maverick. Bulbasaur, after dropping a little girl in a failed rescue attempt in 151 Hidden Depths.
In , Cucumber delivers one as Sir Tomato brings his sword down on him.
Cucumber Quest Sinfest
Pitr of combined this with User Friendly Fake Russian when geeks shot him from moon into the Google building: NYYYYEEEEEEEEEEEEEEEEEEEET!!
Hazel of does a quiet version of this. Girls with Slingshots 
In , the first time Antimony meets the Gunnerkrigg Court Psychopomps, she does this when seeing a dying child's memeory of accidentally burning his house.
: oh gog.
Homestuck : I feel better than alright! I feel great! I feel so alive! I feel so... Trickster Mode Jane CAUCASIAN! Jake: NOOOOOOOOOOOOOOOOOOOOOOOOOOOO
Kanaya's response to Rose asking if she was going to break up with her was a frustrated no punctuated by shoutpoles (!).
Terezi gives one punctuated by crying when Karkat points out that Gamzee will likely leave her.
Taking this to increasingly exaggerated levels seems to have become a running gag. John's big No after a Mind-Controlled Jane kills Karkat takes so long he has to pause for breath at the end of each page before continuing, only for him to immediately perk back up when Jane revives Karkat four pages later. Later, Dave's big no is so long that it stretches down to where his apartment building reaches the lava of LOHAC after Grimbark Jade kicks WV over the edge of the building.
In Ava's Demon, Odin's reaction to seeing his ship.
's Francis Ottoman yelled one when Robbie announced that PvP the party was over.
Ballister gets one in Nimona when a guard cuts Nimona's head off.
In , Tatiana invokes the short, intense version Sandra on the Rocks here when she discovers that something ... terrible ... may have happened to her beloved boss Domenico.
In Questionable Content, this is Faye's reaction to discovering she's officially a New Englander.
Bruno the Bandit Bruno's mother yelled one when her father threatened to turn her granddaughter into a fame vampire.
The spirit of Maledict's brother-in-law yelled one when the ritual intended to transfer it into Bruno's body failed completely.
One of Bran-at-Morn's men yelled one when the Asutonians threatened to burn up their entire laxative supply.
In , believers in Oglaf Sithrak have good reason to take deaths especially hard. (SFW link to a frequently very NSFW comic.)
In , we get this:
Flintlockes Guide To Azeroth Flintlocke: Yeah, har har! Lowping, we beat down this 'ere shaman an' now yeh only 'ave one hit point left!
Flintlocke: Jes' one hit point! Har har! That were awesome!
*Bun'kar sneaks up and nibbles away Lowping's last hit point.*
In , Mary has her Knights Of Buena Vista Player Character do this when another PC is stabbed (it was only 3 damage, but the character wouldn't know that).