8-Bit Theater: Red Mage uses this as Black Mage is about to burn the Rat's Tail they need for the class change. He puts on a levitation spell so he can do the slo-mo jump (but applies too much and is left hanging for a while), and does the Big No just to be dramatic.
Tedd's father uses this when he's realizes he's said he'll pay for eight teenagers to eat pizza, while at a meeting far away. The person he's talking to thinks her ideas are being criticized. The comic is helpfully titled 'I Graduated from the George Lucas Directing Academy'.
Dr. Wily does the traditional NOOOOOOOOOOOOOO! When he realises he's in a catch 22 as the VP of Nexus and doing anything to ruin Nexus would cause Blues to torture him, meaning he would have to go against his nature and save it from the stupidity that was President Haggar.
Alex Willams, replaces the Big No with the Big FUCK, and purposely extends it to buy time to think.
He did a simple Big No before. It lasted something like thirteen comics.
Plays... oddly when Gilgamesh Wulfenbach is led to believe that Agatha was killed by a rogue clank. He begins the wind-up to a Big No before cold logic takes over. However, in a flashback to the same scene, Gil is in the classic "Big No" pose. So it's unclear whether he did one or not.
Many Girl Genius characters get a Big No, of course. Gil demonstrates correct form here.