Big No / Web Animation

• ASDF Movie: "No, llama, NOOOOOO!"
  • "Die Potato!" "Nooooooooooooo!"
  • "Billy, nooo!!!"
    • "Billy, nein!!!"
  • "I AM PUNCHING YOUR SALAD!" "NOOOOOOOOO-"
  • "Oh, NOOOOO--" is quite apropriate just before punching yourself in the face because it's Opposite Day.
    • As well as when someone steps forward for his part in a barberhsop quartet and hears a click, followed by 'Hello!'.
  • "I'm going to punch your face... in the face." "NOOOO—(zoom to face within face)—OOOOO!"
• ATTACK on MIKA: In the Japanese version of Creepy sister..., Kotomi's and Himeka's mom shouts one of these at the sight of a malnourished Himeka preparing to burn down the house.
• Battle for Dream Island: In episode 6, Snowball does this when he gets Rocky and Golfball on his team.
  • Parodied in episode 8. Snowball does this again when he isn't chosen for the relay race. This time, it is sped up to save time.
  • Justified in episode 17, when Leafy accidentally shatters Ice Cube with a frisbee throw and recovers her after answering a long string of questions regarding the recovery, only to shatter her again just before the sun goes down. This causes them to fail the challenge.
  • Bubble does one in episode 8 of BFB when Match demotes her to a Bemb.
  • Snowball does one again in TPOT 1 when the elevator door closes before he and Basketball could enter it.
• Joel of Bonus Stage does one of these from Hell as Satan initiates his punishment… 24 hours a day of Time Squad.
• Green Lantern pulls off a gloriously hammy one when he's trapped inside a yellow smiley face time bomb in DC Super Friends.
• In 'The Camping Webisode' of DSBT InsaniT, Koden does this when he discovered the groups campsite has been ruined, and Duck when Martha accidentally pops Balloon.
• In the Eddsworld episode "Zanta Claws II", Tom has only recently gotten a present of reality eyes. Zanta however is about to kill the real Santa. Tom takes the shot while letting off one of these. The shot hits his eyes, reflects off them and hits a chandelier, crushing Zanta at the cost of his eyes.
  Tom: I hate Christmas...
• FreedomToons:
  • "INFOINITY WARS": Alex Jones lets out a dramatic "No!" as he's erased from existence by an Infinity Glove-wearing gay Pepe the Frog.
  • "Night of the Living NPCs": All Seamus can do is scream "No!" when he's consumed by a horde of NPCs.
• in the end of part 3 of the Frollo Beats Up Evil Residents arc in The Frollo Show, Frollo lets out one after Jafar launches a very, very slow moving fireball at him.
• In GEOWeasel, Weas performs this as he learns that the internet access in Hell is only dial-up.
• Homestar Runner:
  • Strong Mad gets one of these in an encounter with Senor Cardgage in the Strong Bad Email "kind of cool".
    Senor Cardgage: Sorry to bother you, ma'am. Could you help an old Soderbergh gather his spectacles?
    Strong Mad: NOOOOOOOOO!
  • Marzipan gets to say one after being forced to become Coach Z's girlfriend in "Doomy Tales of the Macabre". Since she read the cue card incorrectly, her first attempt was pronounced as a diphthong and sounds more like "moo" with an "n" than "No!"
• HTF +: Flippy does that when he sees LG Creepybloom came back in HTF+Amnesia 3/HTF+LG 4.
• Inanimate Insanity: In "Inanimate Smackdown", Marshmallow does one when she is trapped in Idiotic Island and then encounters Knife.
  • In "Rain on Your Charade", Soap does one after Microphone throws her cleaning supplies off a cliff, but then turns it into a "No thanks".
  • In "Stranded in Paradise", OJ does one when he sees Boaty sinking.
  • In" Snapshot Showdown", Nickel does one after he fails to take a picture of Clover, and doesn't stop until hour ten. He then admits that he didn't stop for a break.
    • In the same episode, Goo does one after Candle takes a picture of him surrounded by pinecones.
• In LEGO Marvel Superheroes: Maximum Overload, Spider-Man does this when Venom points out that he can't eat the last cheese danish because he has no mouth.
• Minilife TV:
  • In "Crossing Ian", Ian lets one out after his bodyguard, Diego Sanchez, is killed by an assassin.
  • In "A Day in the Life of [Melvin] the Ninja", this is Chris's reaction when he finds out Melvin stole his dessert-flavored pudding.
• The Most Epic Story Ever Told in All of Human History: Ridiculously Epic unleashes one at the end of “The Most Epic Supervillain Origin Story” when it turns out that he accidentally helped a bunch of orphans instead of causing the destruction that he intended to.
• My Little Pony: Equestria Girls Digital Series: In "The Last Day of School", Twilight screams this at the beginning and right when the bell rings, followed by Rainbow doing the inversion.
• Grif of Red vs. Blue spends the last moments of the third season screaming "No-!" (seemingly for hours, as said by Donut.), after the Red Team ends up black in Blood Gulch after an endeavor with O'Mally. This extends to the the entire first episode of the fourth season "Familiar Surroundings", with lots of ridiculous plans (devised by Sarge, who else?) that attempt to force him off the cliff he's perched on. The plans are, in order, allowing him to scream until he passes out, and trying to shoot him down with a sniper rifle.
  Grif: NOOOOO!
  Sarge: Simmons! Find a way to get Grif back down here right now!
  Simmons: Alright. HEY GRIF! SHUT THE FUCK UP AND GET DOWN HERE! WE NEED TO CHECK OUT THE BASE!
  Grif: NOOO! NOOOOOO! NOOOOOOOOOOO!
  Simmons: I don't think he's listening, sir.
  Grif: NOOO! ACTUALLY THAT TIME I WAS ANSWERING YOUR QUESTION! NOOOOO!
  Sarge: That does it, Simmons, go get him down now.
  Donut: Sarge, if he keeps screaming like that, he'll pass out, and fall off the cliff!
  Sarge: Cancel that order Simmons!
  Grif: NOOOOOOOO!
  Sarge: (sighing) Donut, get me a sniper rifle.
  • Gets played much more seriously later in the series: in Season 8's penultimate episode, from Church when The Meta stabs Tex and extracts her into an AI memory unit; in Season 10's episode 16, from Alpha-Church, when Sigma (as The Director) tells him Tex is dead; and episode 19 of Season 10, from Carolina as the newly-"born" Meta rips the AIs Eta & Iota out of her skull, and from Tex when The Meta proceeds to throw Carolina over a cliff to her (presumed) death.
• Refreshing Stories:
  • "Manager thought I stole the company's money but...": Bonkura lets one out after Minako demoted him for accusing her son Hiroshi of stealing the company money.
  • "My wife was cheating on me with the delivery guy": Ken Ooshima lets out one after his boss fires him from his deliveryman job for having an affair with Hiroshi's wife, Nanako.
  • "I encountered my wife having an affair while I was on my business trip": Charahiro lets out one when Hiroshi awards him the prize money for beating him at the hotel's table tennis match and proclaims he's going to take it away once he sues the guy for damages.
  • "My wife went on a trip with her boyfriend, so I surprised her by replicating the entire trip": Mana lets out one in prison when Hiroshi told her she didn't look like a human while going berserk in the hotel, in response to her asking if he's going to leave her like that. In the original Japanese version, she just cries.
  • "A colleague stole my girlfriend, became the CEO, and then fired me…" Temae lets out one after his father, the CEO, tells him to work at Hiroshi's subsidiary as a low-level employee in response to his incompetence causing their clients to cancel their contracts with his company.
• Revenge Films:
  • "My wife was cheating on me with her coworker..." C lets one out when he was fired and ordered to pay damages for having an affair with Eiko, Mubio's wife.
  • "My wife wouldn't let our son have his bank book. Turns out she was secretly using it": OP's wife tearfully lets out one when he refuses to hear her pleas for forgiveness after he called her notoriously strict parents to collect her for spending their son Bradley's hard-earned money on her affair.
• Played for laughs in the Robotbox and Cactus episode "Paradigm Shift", where Cactus yells "no" in response to B. Sting saying Robotbox will be his new boss, but then asks, "Wait, you said he'd be my boss?" Upon this being confirmed, he yells "no" again, but seemingly wakes up out of the dream... until he actually wakes up and is told again that Robotbox will be his boss, and yells "no" again before giving a cheesy one-liner.
• RWBY:
  • Parodied and Played for Laughs in the first episode of the second season during a massive food fight between RWBY and JNPR. Weiss has just been knocked out during her duel with Nora, who smashed her right into a pillar at one end of the cafeteria with her melon hammer. Ruby cradles her in her arms in the style of Pietà Plagiarism and screams "No!" at the top of her lungs as the pillar she was smashed into collapses around them, acting as if Weiss had just been killed in action. (Of course, this doesn't actually mean that Weiss is going to die, as she's shown to be just fine seconds after the food fight.)
  • Even Zwei (kinda) gets one in "Mountain Glenn". After tailing the White Fang members - and using a "one bark for yes..." system to signal when they're out of sight - Ruby falls into a sinkhole shortly afterwards. What does Zwei do as he looks down the hole? He barks twice.
  • Played for Drama when Yang sees Adam stabbing Blake and goes full Berserk Mode screaming it as she throws herself at him.
  • It's Parodied and Played for Laughs again in RWBY Chibi, with one example being in its 19th episode when Yang cradles Ruby as she "dies".
  Yang: NOOOOOOOOOOO! She was so young!
  Ruby: Yang... *cough* avenge... me... (makes fake dying noises and "dies" with X's in her eyes)
  • Another example is found in the 21st episode, used by Cinder over the ruining of her evil schemes.
  Cinder: NOOOOOOOO! MY NEFARIOUS PLAAAANS! *cries*
• Sekai no Fushigi:
  • Miyuki has one and breaks down in tears when Daisuke cancels the wedding after he learns of her past affair with a married man.
  • Tokui and Akane both let one out when they found out that Sir Zaitsu is one of their company's clients and the family threatened to report them to upper management.
• Sage from "Shadows of the Past" in episode 20 does this after Eieon mentions they should get Doc and Pinky to help them study the giant computer they found in the humming box. Justified after the ordeal they just went through with two assassins and a giant killer mech one year old freak child. It Makes Sense in Context
• Team Neighborhood: Sniper lets one out in "Bush Beaters" when the SMNBC Sniper steals his Bigfoot kill.
• Ultra Fast Pony:
  • In "Gelatin Swingsets", the Cutie Mark Crusaders are dismayed to discover their coupon for a free gelatin swingset has been taken away, and Scootaloo lets loose a Big No. But since she only speaks scat, it comes out as:
  Scootaloo: Skoooooooooooooooooooooooobobidobidooooooooooooooooooooooooooooooooooooooooooo...
  • In the opening of "The Penny and Clyde Show", the big no pulls double duty as an expression of dismay and and answer.
  Pinkie: Nooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo...
  Applejack: Dammit, Pinkie, would you just shut up?
  Pinkie: Noooooooooooooooooooooooooooooooooooooooooooooo—
• The WTF program in Welcome to... Facebook when he sees what Duck Fayce has done to her profile.