* Grif of ''Machinima/RedVsBlue'' spends the last moments of the third season screaming "No-!" (seemingly for hours, as said by Donut.), after the Red Team [[spoiler:returns to Blood Gulch after an endevour with O'Mally.]] This extends to the the entire first episode of the fourth season, with lots of ridiculous plans (devised by Sarge, who else?) that attempt to force him off the cliff he's perched on. The plans are, in order, allowing him to scream until he passes out.
-->'''Grif:''' NOOOOO!\\
'''Sarge:''' Simmons! Find a way to get Grif back down here right now!\\
'''Simmons:''' I don't think he's listening, sir.\\
'''Sarge:''' That does it, Simmons, go get him down now.\\
'''Donut:''' Sarge, if he keeps screaming like that, he'll pass out, and fall off the cliff!\\
'''Sarge:''' Cancel that order Simmons!\\
'''Grif:''' NOOOOOOOO!\\
'''Sarge:''' ''(sighing)'' Donut, get me a sniper rifle.
** Gets played much more seriously later in the series: in Season 8's penultimate episode, from Church when [[spoiler:[[TheJuggernaut The Meta]] stabs Tex and extracts [[ArtificialIntelligence her]] into an AI memory unit]]; in Season 10's episode 16, from [[spoiler:Alpha-Church, when [[ManipulativeBastard Sigma]] (as The Director) tells him Tex is dead]]; and episode 19 of Season 10, from Carolina [[spoiler:as the newly-"born" Meta rips the [=AIs=] Eta & Iota out of her skull]], and from Tex when [[spoiler:The Meta proceeds to throw Carolina over a cliff to her ([[ReportsOfMyDeathWereGreatlyExaggerated pres]][[DisneyDeath umed]]) death]].
* Strong Mad gets one of these in an encounter with Senor Cardgage on ''WebAnimation/HomestarRunner''.
** Marzipan gets to say one after [[spoiler: being forced to become Coach Z's girlfriend]] in "Doomy Tales of the Macabre". Since she read the cue card incorrectly, her first attempt was pronounced with a long "oo" and sounds more like "moo" with an "n" than "No!"
* In the Eddsworld episode "Zanta Claws II", Tom has only recently gotten a present of [[spoiler: reality eyes]]. Zanta however is about to kill the real Santa. Tom takes the shot while letting off one of these. The shot hits his eyes, reflects off them and hits a chandelier, crushing Zanta at the cost of his eyes.
--> '''Tom''': I hate Christmas...
* In 'The Camping Webisode' of ''WebAnimation/DSBTInsaniT'', Koden does this when he discovered the groups campsite has been ruined, and Duck when Martha accidentally pops Balloon.
* The [[FunWithAcronyms WTF]] program in ''WebAnimation/WelcomeTo Website/{{Facebook}}'' when he sees what Duck Fayce has done to her profile.
* In ''WebAnimation/LEGOMarvelSuperheroesMaximumOverload'', Spider-Man does this when Venom points out that he can't eat the last cheese danish because he has no mouth.
* ''WebVideo/UltraFastPony'':
** In "Gelatin Swingsets", the Cutie Mark Crusaders are dismayed to discover their coupon for a free gelatin swingset has been taken away, and Scootaloo lets loose a Big No. But since [[TheUnintelligible she only speaks]] {{scat|ting}}, it comes out as:
-->'''Scootaloo:''' Skoooooooooooooooooooooooobobidobidooooooooooooooooooooooooooooooooooooooooooo...
** In the opening of "The Penny and Clyde Show", the big no pulls double duty as an expression of dismay ''and'' and answer.
-->'''Pinkie:''' Nooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooooo... \\
'''Applejack:''' Dammit, Pinkie, would you just shut up? \\
'''Pinkie:''' Noooooooooooooooooooooooooooooooooooooooooooooo—
* Sage from "Machinima/ShadowsOfThePast" in episode 20 does this after Eieon mentions they should get Doc and Pinky to help them study the giant computer they found in the humming box. Justified after the ordeal they just went through with two assassins and a giant killer mech one year old freak child. ItMakesSenseInContext
* : ''WebAnimation/BattleForDreamIsland'' In episode 6, Snowball does this when he gets Rocky and Golfball on his team.
** {{Parodied|Trope}} in episode 8. Snowball does this again when he isn't chosen for the relay race. This time, it is sped up to save time.
** {{Justified|Trope}} in episode 17, when Leafy [[spoiler:accidentally shatters Ice Cube with a frisbee throw and recovers her after answering a long string of questions regarding the recovery, only to shatter her again just before the sun goes down. This causes them to fail the challenge.]]
* ''WebAnimation/ASDFMovie'': "No, llama, NOOOOOO!"
** "Die Potato!" "Nooooooooooooo!"
** "Billy, nooo!!!"
*** "Billy, nein!!!"
** "[[MakesJustAsMuchSenseInContext I AM PUNCHING YOUR SALAD!]]" "NOOOOOOOOO-"
** "[[LargeHam Oh, NOOOOO--]]" is quite apropriate just before [[spoiler: punching yourself in the face because it's OppositeDay.]]
*** As well as when someone steps forward for his part in a barberhsop quartet and hears a click, followed by 'Hello!'.
** "I'm going to punch your face... ''in the face.''" "NOOOO--(zoom to face ''within'' face)--OOOOO!"
* [[WebAnimation/{{RWBY}} RWBY]]
** Parodied and PlayedForLaughs in the first episode of the second season during a massive food fight between RWBY and JNPR. Weiss has just been knocked out during her duel with Nora, who smashed her right into a pillar at one end of the cafeteria with her melon hammer. Ruby cradles her in her arms in the style of PietaPlagiarism and screams at the top of her lungs as the pillar she was smashed into collapses around them, acting as if Weiss had just been killed in action. (Of course, this doesn't actually mean that Weiss is going to die, as she's shown to be just fine seconds after the food fight.)
-->'''Ruby''': Weiss, Weiss! Don't leave me! NOOOOOOOOOOOOOOO!!!!!!!!!!!
** PlayedForDrama when Yang sees [[spoiler: Adam stabbing Blake]] and goes full BerserkMode screaming it as she throws herself at him.
** It's Parodied and PlayedForLaughs again in [[WebAnimation/RWBYChibi RWBY Chibi]], with one example being in its 19th episode when Yang cradles Ruby as she "dies".
-->'''Yang''': NOOOOOOOOOOO! [[TooGoodForThisSinfulEarth She was so young!]]\\
'''Ruby''': Yang... *cough* avenge... me... (makes fake dying noises and "dies" with [[WingdingEyes X's in her eyes]])
** Another example is found in the 21st episode, used by Cinder over the ruining of her evil schemes.
-->'''Cinder''': NOOOOOOOO! MY NEFARIOUS PLAAAANS! *cries*
* in the end of part 3 of the ''Frollo Beats Up Evil Residents'' arc in ''WebAnimation/TheFrolloShow'', Frollo lets out one after [[spoiler: Jafar launches a very, very slow moving projectile at him.]]
* Joel of ''WebAnimation/BonusStage'' does one of these from Hell as Satan initiates his punishment… [[CoolAndUnusualPunishment 24 hours a day]] of WesternAnimation/TimeSquad.
* In ''WebAnimation/GEOWeasel'', Weas performs this as he learns that the internet access in Hell is only dial-up.