Agents of S.H.I.E.L.D.: Alphonso "Mack" Mackenzie, so much so that roughly half of the entries on the series' Ho Yay page involve him in someway, especially in his interactions with the Ambiguously Bi Fitz, who are currently enjoying Fan-Preferred Couple status on the Internet. Most of the questions surrounding Mack's sexuality come from the fact that when he discusses his exes with the team during one episode he never specifies the gender of his former significant others, and it seems very deliberate on the writing team's part. His only significant relationship with a woman is with Bobbi. Then again he does start up a relationship with Elena so perhaps he falls under Ambiguously Bi.
Lieutenant Gruber who had very camp manners and openly courted René. Word of God confirmed he's gay during a reunion special, but the Where Are They Now epilogue leaves it ambiguous by showing that he married Helga after the war and had children with her.
General von Flockenstuffen is said to be "a bit this-a-way and that-a-way."
Are You Afraid of the Dark?: Sardo, if you go by stereotypes. His effeminate mannerisms such as earrings, a high voice and quasi-obsessive tidiness could be interpreted this way.
Are You Being Served?: Mr. Humphries. He was effeminate, camp, and often talked about his dubious 'leisurely activities', yet it was still hinted that he might actually be straight. John Inman, the actor who played him, came out of the closet years after the show aired. Ironically, Word of God from Inman himself was that Mr. Humphries actually was straight.
Tobias. The (often) very obvious homosexual undertones in his lines are possibly the show's longest running gag.
"I wouldn't mind kissing that man between the cheeks, so to speak."
In Season Four, a lot of Gob's more eccentric relationship behaviors in the first three seasons retroactively became Ambiguously Gay. Series creator Mitch Hurwitz has confirmed several times in post-s4 interviews that Gob is gay, but that Tobias is straight.
Amy. While she is in a committed (if sexless) relationship with Sheldon, she has a tendency to make some comments about her "bestie" Penny and her beauty. And there was the time she got drunk and kissed her. And the time she wanted to have a bachelorette party that involved stripping naked and painting on each other. Also the time she said Penny had a better chance of getting with her than Leonard did. The list goes on. Of course a lot of it is simply social awkwardness and over expressing her joy at actually having friends, especially a friend with social graces like Penny (aka the resident Cool Big Sis). Amy is more Ambiguously Bi.
Gus Fring. His relationship with his former business partner Max Arciega was extremely close and he endowed a chemistry scholarship in Max's name years after his death. Word of God has said this is a valid interpretation of their relationship.
Andrew was the king of this trope before he came out as gay in the canon comics continuation. He all but said he was in love with Warren, and did as he said because "He promised we'd be together, but he was just using me. He never really loved... hanging out with us." (He changed direction of that last sentence rather uncomfortably, so it seems likely he was closeted). He has rather clear moments of lust over Spike, Xander, and Jonathon Archer. Word of God confirms that he was at least bisexual, but never got around to acknowledging it to himself — thus why it was never acknowledged on the show. The actor who played him is gay, so had some fun with adding this to the character. Then there's his Angel appearance (where he was flanked by two Ms. Fanservice examples), and then again in the comics, which had him as unimpressed with walking in on two girls making out and implying that his Angel cameo was a feint.
Andrew: *On seeing Spike and Anya have sex on a table* He [Spike] is so cool... [beat] And, I mean, the girl is hot. Too.
Jonathan seemed very close to Andrew, especially when they were in Mexico, where they slept in the same bed.
The writers couldn't decide whether Willow or Xander should be gay at the start of the series, so both of them fell into this category at times (most notably, Xander being Mistaken for Gay by Larry, and Willow saying "I think I'm kinda gay" in reference to her doppelganger). Eventually, Willow came out and Xander became purely heterosexual. Xander still had moments of this after Willow came out, memorably in "Intervention".
Lorne, the Pylean demon from Angel, may be a much more subtle example of this trope. He constantly flirts with "Angelcake", but then again, we don't even know what gender he is, given that his mother looks like a huge bearded man. The actor stated that he always saw Lorne as an asexual character who enjoyed being alone with a few close friends.
Combat Hospital: Dr. Grace Pedersen. When a female character flirts with her and asks her out, she only refuses because she's already taken (never mentioning said partner's gender), and she also claims to have her appetite for men spoiled by her husband.
Community: When the Dean has to pretend to be gay for political reasons (or more specifically, just gay), Frankie Dart mentions that she doesn't want her sexuality to define her. Jeff and the Dean share a look, and realize they have no idea what Frankie's preferences are. Later episodes reveal that the rest of the group has a pool going, which pisses Frankie off when she finds out about it. Frankie gets tiny bits of Ship Tease with both Jeff and Annie, but the moments are small enough that she could just be worried about their codependent relationship.
Dan for Mayor: One episode featured the titular Dan trying to win the support of an ambiguous conservative for his campaign. As he tries to interrogate the real estate agent for clues on his sexual orientation, the answers he gets are all the more ambiguous.
Eureka: Vincent. He's camp, he comments on a male character's attractiveness in the finale, and on the hypothetical attractiveness of "Joe" when he's forgotten Jo exists, and when the other male characters are talking about wanting to kiss Beverly he says "Even I've thought about it", implying this is unusual. Apart from that, he's more concerned with his cooking than relationships of any kind.
Gill. Ambiguously Gay from day one, was married to a Butch Lesbian, then suddenly became gay. His producer as well, whom Gill admits he's never seen with a woman before.
Niles on Frasierexhibits all the stereotypes, except that he's clearly deeply enamored with Daphne. Niles fits the trope so well in-universe that other characters occasionally assume he's gay (either not knowing about Daphne, or figuring that she's The Beard).
Frasier was Mistaken for Gay as well on a few occasions. At one point, he assertively tries to get his new boss to date a lonely Daphne, but doesn't realize the man is gay and thinks Frasier himself is the one pursuing him (it also turns out they have a lot in common). Even the great Patrick Stewart became his sugar daddy for an episode, and Frasier of course was oblivious as to what was really going on until the very end. Reached critical mass when he was trying to help Niles find a wedding ring for Daphne, and the way the two bickered about and then agreed on a ring in the store was greeted by a round of applause with everyone thinking they were a gay couple, to Martin's exasperation.
Game of Thrones: As in the books, the eunuch Lord Varys seems to have what might be a romantic interest in his "little birds," which are young boys uses as spies. When the bisexual Oberyn questions him openly about which sex he preferred before his castration, Varys claims that he preferred neither boys nor girls and never had an interest in desire. This may simply be a convenient lie or coping mechanism, but Varys also claims to have been castrated as a young child, before he would have experienced any sexual desire, so it's possible that he's telling the truth.
Gavin and Stacey: Bryn certainly shows a lot more interest in men than women, but it's never confirmed as he's entirely chaste and implied to be a virgin. Given that he's unfazed by other people's homosexuality, it seems not so much that he's in denial as that the thought he is gay has just somehow never occurred to him.
The Get Down: Shaolin Fantastic ("The Lady-Killing Romantic") has a LOT of Ho Yay with protagonist Zeke, which a lot if characters directly reference.
Mylene: Maybe you have the hots for my boyfriend (Zeke)
Gilmore Girls: Michel hit every Camp Gay stereotype (obsessed with his appearance, dotes on his mother, French!) but he did once mention a girlfriend (who went unseen).
Kind of a Running Gag with the Dalton Academy Warblers, as people assume that they are gay. All of them. One of the judges at a Regionals competition asks if they are a school for gay boys. Even Blaine seems a little unsure about it, at one point in the sixth season asking if any of them are straight when they protest a girl trying to join their choir. For added humor, whenever the question gets asked of one of them directly, the Warbler who answers is almost invariably Camp, yet denies the allegation vehemently.
The Good Wife: Kalinda. The show likes to be ambiguous about it (or pretend that they are being so), but even though Kalinda has made out with guys while on cases, it seems strongly implied that she prefers ladies on her own time.
Gunpowder: As in real life, it's unclear whether King James I of England and VI of Scotland has homosexual relationships with his favorites in court or whether they're just extremely close friends.
Danny the cultured fisherman. He doesn't appear to have any interest in women, and, when asking Kenny to come to a posh restaurant with him, it sounds like he is asking him out.
The Posh Scaffolders. They both act camp and very rarely discuss women (outside of heckling them, which they only do, according to a tearful Darren, because they were 'trying to be friendly'). They are also constantly touching each other. Kev frequently calls Darren 'dear'.
How I Met Your Mother: Ted's boss Hammond Druthers goes through a nervous breakdown and it's implied that it's because of repressed homosexuality, most notably manifested in his designing a skyscraper shaped like a penis which he refers to as "the male organ of love" in a shaking voice. He also lovingly describes his wife as mannish.
Heroes: In season one, Claire's friend Zach is never explicitly stated to be gay but all of the hints are there, and his big "I know who I am - I like who I am" speech doesn't leave much to the imagination. Then the whole "best friend has super powers and a serial killer after her" plot took precedence, his character was ignored and then he disappeared altogether.
iCarly: Sam is either this or Ambiguously Bi. She seems to like guys but none of them ever last past one episode, including the one in "iMake Sam Girlier" that such a big deal was made over. That episode also seemed to lampshade Sam's Butch Lesbian behaviors. Also, she and Carly have a lot of Les Yay. There is also speculation that Carly's brother Spencer might be gay, because of the episode where he was wearing a dress and dancing with cute guys saying, "I think I like it."
It's Always Sunny in Philadelphia: Mac, for much of the series. While he's initially an unambiguous straight guy with an adolescent fixation on buff action stars, as the series goes on, more and more jokes suggest that Mac is a closeted gay man whose Catholic faith causes him to be an Armored Closet Gay. In season 12 episode 6, the ambiguity is finally removed.
iZombie has a meta example with Evan, Liv's brother. Due to time constraints, a scene where he comes out to Liv was removed from season 2. Because the canon status of the scene was never cleared up, neither was Evan's sexuality.
Tim Gutterson is a possible example. In "Veterans", he openly flirts with a (male) bouncer outside of a bar.
Tim: I love this shit. This shit makes me hard. Raylan: Well, now we've both been warned.
On the villainous end of things, Wynn Duffy has an odd speech pattern, an obsession with women's tennis, sleeps in a beauty mask, and lives in his trailer with his male bodyguard, Mikey.
Just Shoot Me!: It's made fairly clear to the audience that Finch isn't gay, but his family have their doubts (most of the other characters though only played along to annoy Finch). Lampshaded in "Pass the Salt", in which one of his macho big brothers is revealed to be gay instead.
Keeping Up Appearances: It was a running joke that Hyacinth and Richard's son Sheridan, who lives with a man named Tarquin (who makes his own curtains, wears silk pyjamas, and has won prizes for embroidery), is possibly gay. Hyacinth however seems blissfully oblivious to the fact, and its heavily implied that Richard either simply pretends not to know for his wife's sake or just doesn't care if his son is gay; he's only bothered that he keeps phoning to ask for money.
The Larry Sanders Show: David Duchovny appeared twice against type as a version of himself whose words and mannerisms constantly had Larry wondering if he was coming on to him.
The Latest Buzz: Michael Davies. Being a kid's show, they can't actually state that he is gay but he is teenaged boy who writes the magazine's gossip column, is obsessed with fashion, and loves musicals (and sings the female parts). In second season they tried to move away from this characterisation by toughening him up and giving him an interest in girls, with varying degrees of success. In one episode he seems over the moon when a male student compliments his tie, until another character points out that he might have been being sarcastic. Michael then spends the rest of the episode obsessed over whether or not this guy really likes his tie, seeming like he'll be crushed if it turns out he didn't.
LazyTown: Robbie Rotten. Despite claiming to hate Sportacus and wanting him to leave the town, Robbie sure spends a lot of time talking about Sportacus, touching Sportacus and jumping into his arms whenever the slightly-above-average hero shows up.
Lewis: Sergeant James Hathaway of refuses to draw neat lines between sexualities or tell Lewis his own orientation. A former seminary student before joining the police, Hathaway seems conflicted about religion and sexuality.
Lost in Space: Dr. Zachary Smith who spoke in an effeminate manner was a hopeless coward and whose only real friends were a robot and a young boy.
Mad Men: Salvatore falls under this trope for about six episodes before he's resolved as, ah, unambiguous, but that has more to do with the decade he lives in than with maintaining any kind of tension. Compare this to the episode where a more minor character, a copywriter from Europe, very unambiguously outs himself to his coworkers; one look at Sal's face tells you that he was comparing their situations.
Malcolm in the Middle: Dabney and Lloyd, Malcolm's two effeminate classmates who like to talk about his eyes and think he is "handsome".
It's obvious to those in the universe; Cynthia even brings it up while she and Malcolm are watching the two of them eat lunch. Cynthia claims she can read lips, and fills Malcolm in. It's never determined if she can actually read lips or not.
Reese, particularly in the latter seasons as well.
As James Gillies and his best friend Robert Perry depart from the police station for the first time in "Big Murderer on Campus", James touches Robert's back in a manner which may suggest that there is a greater intimacy between the two of them. Brackenreid wonders out loud, "Just good pals, or something more?" Even less ambiguous by Season 7, where Gillies kisses a struggling Murdoch straight on the lips. However, it could have been just his strategy to shock him as it was his last shot at escaping.
Mr. Carducci in "This One Goes to Eleven" is a dandy and has many flamboyant mannerisms, of note, James Pendrick comments that Carducci "...takes an interest in young, male painters".
Katie in Sweet Polly Oliver episode "Victor, Victorian" seems highly interested in the fact that Julia is "an unwed doctor living alone", mentions that marriage leaves her "bored and unhappy" and has no qualms about inviting Julia into the secret club. The inevitable betrayal by Julia gets the doctor a disapproving glare from Katie and called "you rat" (not unlike the reaction by Jeffrey when his gay tennis club is busted by Murdoch, in whom he had taken an interest in "Till Death Do Us Part").
After the bombing at the start of "War on Terror", Constable Crabtree and Dr. Grace are canvassing the neighbourhood shops when the doctor tries on a hat at a milliner's establishment. The fastidious milliner scolds her sharply for even touching the merchandise, then immediately turns to fawn over the constable, encouraging him to try on a hat and urging the reluctant Crabtree to come to his new shop's opening gala moving sale. As they're walking away, Emily observes that George's forceful manner was more successful at getting the man to give them information than anything she could do. Later, Emily slyly says the shopkeeper would be disappointed to know George is attracted to women.
Mystery Science Theater 3000: Depending on the episode, Servo and Crow can qualify. In one episode (Racket Girls/Are You Ready For Marriage?) they try to get married and end up with the entire cast in a wrestling match. However, considering they are robots, they may not really understand love.
"Brain Guy" Observer was a bigger example of this trope, going into the ladies' restroom with Pearl, dressing up in a stereotypical leather outfit for a "hot date", and refusing to seduce a woman:
Observer: Oh oh oh, no no, I could never seduce a woman, Pearl. Pearl: Oh, because you're... Observer: I thought you knew! I am completely, utterly... without a body. Pearl: Well, thank you for coming out with that information.
The Office: Michael Scott. His unusual obsession with Ryan the temp leads to hilarious moments, such as saying he would have sex with Ryan during a 'who would you sleep with' game, and disturbing moments, like intently peering at Ryan behind the blinds of his office space. Came to a head in Gay Witch Hunt when, to show he is tolerant of gays, tried to kiss Oscar against his will.
That's Just Me: Monica always pretends to be straight and says she's both bisexual and asexual, although her more masculine traits says something else....
Elizabeth's ex-boyfriend Steven loves pink and yellow and girly shows to the point of almost everyone questioning his true sexuality. Even Elizabeth makes fun of him for it.
Also, he tricked Elizabeth for a long time by pretending to be a woman named Stephanie....
Most girls on this show are super close. Bridget and Joanna have a fake marriage with Anika, Monica is the submissive worker of Hannia, Bridge, Anika, and Tsering, and Lela says some weird things to Joanna...
Most notably was in We're Moving?! But We'll Need a Truck was when she hugged Joanna from behind and asked if she wanted a massage.
One Big Happy has Kate when the group first meets her. They decides to play "Gay or Nay" to determine whether Lizzy should pursue her or not.
Outlander: The Duke of Sandringham, who is stereotypically effeminate (although this is similar to how many straight "fops" or "dandys" acted), a lifelong bachelor, and played by the openly gay English actor Simon Callow.
Power Rangers Lightspeed Rescue: Kelsey. While she is on the femme side of butch, she is an extreme sports junkie, and she got very close to an astronaut named Nancy. She also once offered to teach Chad how to talk to girls. It's debatable, though, as when Chad said Marina was "the one" for him, Kelsey is clearly upset. She could just be feeling sorry for her friend, or...
Vida from Power Rangers Mystic Force, on the other hand, is very awfully butch for a ranger based on a butterfly (it was a fairy in Magiranger, by the way), was made into a Lesbian Vampire, and at the end of the series when said vampire was turned human noted loudly how hot she became.
On Power Rangers Ninja Steel, it's sometimes hinted that Monty (one of Those Two Guys) is attracted to Victor. He compliments Victor's appearance, thinks he's awesome, and acts like his personal Yes-Man even when Victor is being a bungling idiot (which is often). Monty even looks a bit like a teenage Smithers.
Alison constantly flirts with Emily , the (then) closeted lesbian who was in love with Alison. It's unclear whether Ali was faking all of it, as Emily was something of a Morality Pet for Alison. Later in the series Alison admitted that she basically never loved anyone except Emily
Mona, who became A for the sole purpose of having Hanna all to her self. The show never explictly states whether or not this is in a romantic way, but Mona does seem very obsessed with Hanna.
Parodied this with the "Lyle the Effeminate Heterosexual" sketches, in which the title character would lisp and mince and prance his way through the sketch — and then object loudly whenever somebody insinuated he was gay. Among those led astray by his mannerism were his wife and son.
Mango, played by Chris Kattan. Mango is a male exotic dancer who performs in a strip club. Though effeminate, Mango is adamant that he isn't homosexual, claiming that he stripped to support his wife and children. For what it's worth, one sketch showed his wife and children on screen. Of course there was also the West Side Story-cum-Grease parody involving him in high school, where one of his song lines was "Boobies give me the creeps." It seems to be whatever is funniest at the moment.
The Ambiguously Gay Duo originated as a animated sketch on SNL and, as such, are detailed further in Western Animation.
Seth Meyers on the Weekend Update segments with New York City correspondent Stefon (played by Bill Hader). Meyers always tries to rebuff Stefon's advances by saying he has a girlfriend, except for the last segment on the season 38 episode hosted by Ben Affleckwhen Stefon breaks up with Seth and Seth stops Weekend Update to stop Stefon from marrying Anderson Cooper.
Stuart Smalley of the Daily Affirmation sketches in the early 90s, and later, his own movie, is known for being very effeminate, but his orientation is never flat out stated, but it is hinted that he's possibly gay, and any romantic partner mentioned usually has a Gender-Blender Name, such as Dale, Chris, or Merle. His father also once called him Liberace.
Scrubs: The Todd began as a hyper-straight chauvinist pig. Later episodes heavily implied he was either a repressed or overcompensating homosexual. Then the hints were that he was a full-blown omnisexual unconcerned with any orientation, until one episode explicitly stated it.
After the Todd appreciates half a dozen widely varying people in as many seconds... Janitor: What are you? The Todd: I'm The Todd
This issue has, of course, come up among critics of Sesame Street a lot (of all places) regarding Bert and Ernie. In fact, in recent years, when more gay celebrities have been willing to come forward as such, many LGBT rights leaders petitioned PBS to finally come clean about it. PBS made a public reply in the negative: Bert and Ernie are just close friends.
Brian Topp, who when questioned as to whether he's gay or not replies in the negative, but in a thoughtful tone of voice that suggests that if he's not gay, then he thinks he should be. Many episodes deal with his love-slash-lust for one of the female characters, but one episode also deals with the return of his platonic-love heterosexual ex-life partner.
Played up in several episodes, such as the one in which he "comes out" as an artist to his mother, who had been under the impression he was a lawyer because he thought his parents would disapprove of art. Her cheerful response - "Don't be silly, your father and I don't mind what you do, as long as you're not gay!"
Spin City: One episode featured the Mayor's nephew, who was basically a walking Camp Gay stereotype that loudly and obnoxiously hit on women at every opportunity, worked as a "closet organiser" and said the word "closet" roughly every three lines. Subverted at the end when he confides to Carter (the local token gay) that he was in fact 100% straight, but adopted an elaborate deception to create ambiguity as to his sexual orientation, because his parents desperately wanted to set him up with a partner (of either gender), and he preferred being alone.
Stargate Atlantis has a strange case - one of the writers has stated that he sees one of the recurring characters as being gay, but we don't know who he is. The leading theory among fans is that it's Major Evan Lorne, Sheppard's second in command who has no canon love interests.
See also the more extensive entry for Garak under Always Camp.
Also it was explicitly stated by the actor later on that he played Garak as omnisexual.
The actor also explicitly said that he played Garak as being attracted to (male) lunch partner Julian Bashir, but with the understanding that Bashir never recognized it.
Supergirl: Alex Danvers was suspected by many fans to be gay as early as the Pilot. Though a male character expressed interest in her in the first season, she never returned that interest. While she had good reasons not to be into that character, she lacked any significant love interests in her backstory. She mentions having dated men in college, but she doesn't seem to have actually liked any of them. When DCTV revealed that a character on either Supergirl or The Flash (2014) would be coming out in the upcoming season, Alex was an immediate suspect. As of CrossfireAlex's sexual orientation is no longer ambiguous.
Tales from the Crypt: The mannerisms and speech of Beaks, the assistant of insane stage director Nelson Halliwell from the episode "Top Billing", just scream "gay". Of course, it could also be because he's insane as well.
Teletubbies: Tinky Winky carries a handbag, has a triangle on his head and is purple. He even wore a skirt in one episode. Parodied in The Simpsons when Homer dresses as one.
I'm Homie Womie, the Teletubbie. And I'm all man in case you heard otherwise.
Trailer Park Boys: It is heavily implied throughout the series that Julian is gay, his type being musclemen. in the "Out of the Park: Europe" season the last half of the Helsinki episode covers it almost nonstop!.
Justin and Luigi Lombardi from the Mexican version La Fea Mas Bella; the Italian name is because Camp Gays in Mexico are usually given these kind of names. Interestingly, Mario Lombardi from the original Colombian version was more open but nothing near to flamboyant, having comparatively few mannerisms while talking about his boyfriends and male lovers quite openly; it seems that, in his case, calling him "gay" openly was a bit redundant. And Justin has the unique distinction of being, at most, thirteen years old.
When the show premiered, the actor playing Justin was just 11, and his Ambiguously Gay characteristics were just a running gag. Once he got to high school age, the show starting fleshing it out more, with Marc, an openly gay character, becoming something of a mentor for him. After several arcs that never used the word "gay" but always heavily implied it, Justin was the first to speak openly about the issue by flat out denying it. However, when he and another boy started fighting over a girl, it became clear that they were actually more interested in each other, with their tension finally culminating in a kiss which finally brought him out of the "ambiguous" category.
Main character Tori is popularly thought to be this or Ambiguously Bi, with those comparing the more bromantic way she acts around boys (despite a few minor one-episode boyfriends) to her often flirty interactions with her female friends, guest stars and background characters and deducing it as a case of Hide Your Lesbians, not entirely implausible for this being on the for-kids network Nickelodeon. Subverted during season 4/3.5, as perhaps some executive or creator meddling gave her a noticeably contrasting Girly Girl makeover and many boy-centric songs to sing, though she still has a ton of Les Yay moments as well.
Robbie was definitely thought to be this early on, though as the series progressed and the popular beta ship with Cat got more heavy teasing, it turned more towards Ambiguously Bi.
Wizards of Waverly Place: Fans who don't think Alex is in love with Justin or Mason tend to think this of her, based on how she acts around other girls. Particularly with Stevie, who is less ambiguous.
The Young Ones: Some fans view Rick this way as he's never been with a woman neither have the others but that's another story, has a very effeminate personality (along with feminine articles of clothing and purses), reads women's magazines, has a small obsession over one of his male teachers (along with Cliff Richards), gets personally offended over homophobia, and is a closeted cross-dresser.