"And I shot 'im in the ass, with one of these! What? I did."
Deadpool: Logan. We missed you. The Weapon X isn't the same without you. Nobody calls me "bub" anymore! And Omega Red's a bed wetter! [just then Omega Red enters with the Doc] Omega Red: One day I will tear out your flippant tongue, Wilson. Deadpool:[whispers] He's very ashamed.
Deadpool loves pointy things.
Professor: We put considerable time and money into you. Deadpool: And pointy things!
"You know, I'd would think that the last thing he said was, 'AAAAAAAAAAH, Sabertooth!'"
Which is followed by Wolverine grabbing Deadpool, saying, "He's all yours, Einstein!" and launching him at the Hulk.
Hulk and Lady Deathstrike's yelling contest deserves a mention.
The fact that Wolverine eggs on the Hulk to destroy the Weapon X facility... only to realize Hulk is still quite pissed with him and bats him out of the facility with the support beam.
On a meta note, Fred Tatasciore and Steve Blum recorded together and had to be pulled apart slightly, because the pair were acting it up so much that the staff were worried they'd both clock each other.