Marcus in 1/0, a little bit, before he gains a Fourth Wall, and later to the point of insulting his son for his insufficient "structuration". Junior is also, though he isn't sure what to do about it.
Despite the original intention of his being a straight-man, Pennsylvania GovernorDoctorZexion Doreone of Ansem Retort has grown to be the comic's most prominent Jerkass in a comic full of them. He's helped hijack a plane, embezzled taxpayer money on hookers, planted explosives in his patients, and generally ruined the lives of most everyone around him. The only person that seems to enjoy Zexion's company is Axel, a psychotic mass murderer (who saved Zexion from impeachment by Phoenix Wright by threatening the judge and jury ... after killing three previous juries).
Axel and Darth Maul. Yes, THAT Darth Maul. Namine and Aerith help Zexion in his administration though.
Scott Meyer has commented, multiple times, that the character he based on himself is essentially one of the villains of his comic, singling out comics such as How To Talk A Friend Through A Problem where a friend comes to him for advice and is rewarded with smug mockery. That being said, Comic!Scott still manages to be somewhat sympathetic considering what he has to deal with.
Mullet Boss is a greedy, sleazy asshole with a particular line in forcing Scott and the other employees to do his work for him, making them stay late or otherwise mess with them for his own amusement. His antics have included using terrible food to fill the break room with noxious gases to get everyone back to work, forcing Scott to come up with pointless things for Scott to do, and making the employees badmouth each other, and just for bonus points he's both an Unsatisfiable Customer (demanding free refills even when they're not offered and antagonising the staff of fast food places until they snap and yell at him so the manager will give him the food for free) and That Guy who always brings a guitar to parties for attention.
Nobody, not even Mullet Boss, likes Jenkins. An endless font of sexual harassment towards anyone female in the area, an awful coworker who never puts in his fair share of anything that needs to be done, a weirdo who reads tentacle porn on his phone at work, a prick who will throw you under the bus for any reason at all, a leech on the energy and resources of everyone around him, a noxious presence just in terms of general hygiene and behaviour and just a genuinely unpleasant person who makes the experience of interacting with him an ordeal at every turn.
Jenkins: (on throwing himself a surprise party) All I had to do was lure all of you here, which was easy, since I owe each of you money, a favour, or an apology. Scott: You owe many of us all three. Athena: And if you didn't, you probably do now.
Bomango: Gogo often goes into a violent rage. She's also socially inept to the point where comes across as more immoral than awkward. Her twin, Didi, once called her "[...] Womanchild who throws trucks when she's angry." However, Gogo is often shown to have a Heart of Gold underneath her mean violent exterior.
Chex from Checkerboard Nightmare. He seems to be incapable of empathy, and have a loose grasp on reality in general. He only seems to get away with it because of Rule of Funny.
Cucumber's dad, Lord Cabbage, has a jerkface rating of 6 out of 5 in one of his bios. He also seems to enjoy telling people about the first time Cucumber failed him miserably, is gleefully unsympathetic about upending Cucumber's life, and openly favors Almond.
The Dream Oracle—the purported Big Good—is rude and dismissive towards the heroes, doesn't even bother getting their names right, and spends a lot of time dodging responsibility for her duties and her screw-ups.
Demonseed Redux: Even before becoming an antagonist, Rhoda asks Chico to ditch all his friends, Dee included, and stay with her. When she attacks Dee, Chico figures out she didn't love him in the first place. As a demon henchmen, she thinks about getting back at Dee even if she's been told to capture her alive.
Both Thief and Black Mage from 8-Bit Theater. Sarda also fits, since he's an omnipotent chaotic Trickster Mentor (an example is when he threw Australia on Black Mage, not only to punish him for being disobedient but also because the resulting wave would destroy the city White Mage was helping reconstruct).
Thief is a more intellectual type of jerkass mainly motivated by greed and feelings of elven superiority, who loves screwing people over with legal contracts and l33t Ninja skills. Black Mage is, by all accounts, a smelly sociopathic fat little creep with horrible manners vis-a-vis women, to the extent that (it is said) the universe exists to hurt Black Mage. On the other hand, many people would love to kick Thief in the groin face, but ever since he upgraded his class to ninja, he's (nearly) impossible to hit.
Elf Blood has Gipsy, a human sorceress who generally treats everyone like crap unless they hold some sort of authority over her.
Freefall has Mr. Kornada. His introduction features forcing Sam and several robots to abandon Florence to potential death so he could reach a meeting, after she had saved his life. He later moves on to nearly destroying the entire colony so he could get his hands on the robots' assets, feeling it horribly unfair that anyone else has money at all.
Girls with Slingshots has many jerkasses in its cast, but the absolute queen of them all is Candy, who early on attempts to rape Chris and refuses to forgive him for daring to resist and escape, goes out of her way to sabotage her supposed best friend Jameson's relationship with his girlfriend Maureen because she's in love with him herself, and eventually becomes a professional dominatrix, which is probably the least jerky thing she's done. Her hatred for Maureen culminates in her attempting to sabotage her wedding to Jameson. It backfires. Gloriously.
Turner in Godslave act like this. Even though he's the one who dropped a very important jar, leading to its breaking, he starts to verbally chew Edith for it.
Guilded Age: Payet Best. He even unlocks an achievement for it.
Reynardine from Gunnerkrigg Court is... difficult to categorize. When he meets Antimony, he tries to steal her body knowing that it would kill her but ends up in her stuffed toy instead (making her The Kid with the Leash). For a while he alternates between surly and pleasant, disruptive and helpful, lewd and respectful towards his owner. Over the course of many chapters, he becomes a trusted friend bordering on a Parental Substitute for her, leaving the jerkness behind.
Karkat is characterized as an angry and misanthropic Jerkass, (theoretically) like everyone else of his kind.
Vriska is a particularly horrible example of a Jerkass because the first thing we see of her is the incident in which she runs Tavros off a cliff with mind control.
AG: Apologiiiiiiiize!!!!!!!! AG: Say you're sorry for being a cripple! Wheeeeeeee! AG: Aaaaaaaahahahahahahahaha!
Eridan is quite the Jerkass- not only is he hugely self-obsessed and just generally obnoxious, but he is also a strong proponent of killing all the lowbloods. Which he eventually partially succeeds at doing.
Kankri, Karkat's ancestor, is a very subtle kind of jerk. He constantly avoids triggering or insulting people directly, but he does so in such a condescending and faux-intellectual manner that he winds up insulting them anyway. He's a bit of a hypocrite too.
Cronus. It says a whole lot that he is creepier than Eridan.
Meenah, although it's been shown that she is not the worst member of her group and does have Even Evil Has Standards moments.
Then there's Caliborn, who's kind of a sadistic prick even at the best of times.
Trigger-happy Men in Black Agent Jerry in The Inexplicable Adventures of Bob!. As essentially a recurring villain or at least obstacle, Jerry isn't supposed to arouse much audience sympathy. At least Agent Ben gets along with him.
Rayne from Least I Could Do most certainly qualifies. Self-centered, self-absorbed, and prone to assholery at the drop of a hat for his own amusement. He frequently crosses over into Jerk with a Heart of Gold territory.
Mage & Demon Queen: A lot of fire mage Malori's fellow adventurers are jerks, and she has little compunction about turning them into scorch marks if they give her lip.
Megaman in Megamanspritecomic is an asshole to everyone he meets with the exception of Zero.
Most of the characters in Ménage à 3 are somewhat morally ambiguous and can be rated as jerks, although they usually manage to at least Pet the Dog occasionally, and probably rate as jerks with hearts of gold. See the comic's Characters page for assessments of all the significant characters, many with reference to some jerkass-related tropes. But to be going on with...
Sonya, the subject of some of Zii's early thoughtlessness, can be seen as Zii's actions having incarnate consequences. Her Serious Business approach to seduction can make her as bad as Zii, for whom she becomes a Stalker with a Crush (taking the role over from Yuki), and she can be really petty and bitchy at times — sexting Gary while he's on a date with Kiley, putting Gary's hands on her breasts to purposely unleash Yuki's fury on him so she can make a move on Zii, pulling similar moves on Gary to purposely provoke Yuki, gloating over Zii's break-up with Erik... She manages flashes of kindness, though; her worst features mostly derive from her obsession with Zii. Subsequent events leave her just plain confused.
The initially sweet DiDi's obsessive search for an orgasm sends her across the line a few times, mostly because she sees Gary as her best prospect and won't let much get in her way; trying to break up with Matt by text message, seeking to undermine Kiley, and taking every opportunity to get seductively naked with Gary, for example. Then she gets her orgasm from another source — and won't let anything stop her from getting more of the same.
On the male side, we have Jordan, who basically mindscrewed Gary into confusion about his own sexuality; Matt, who at least admits he's a bastard; Dillon, whose own obsession with Gary soon gets out of hand; and the just plain obnoxious minor characters Nathan, Takeru, and the amazingly creepy Yvan.
Cassiel is willing to beat an innocent partygoer over the head and strip her naked to get into a party to make life difficult for Ash and destroy Rumisiel. Maybe it's that her uncle is Lucifer that makes her act that way or maybe it's because no one will let her forget it.
Sheldon is an egotistical, arrogant, and manipulative jerk who abuses his old friends' trust, even stating outright that they're just 'dumb muscle'. He also seems out to claim as much influence as a driver as possible, kicking people off their own roads in racing gambles and using his muscle to ensure they stay off. Also doesn't take kindly to losing a race/bet—when Ash beats him, he smashes his own car up with a rock to make it look like he was on the receiving end of cheating. Fortunately, he's given a Humiliation Conga he won't soon forget.
Heather has made it her mission in life to destroy Ash because her crush liked Ash better. So far she has failed miserably in all her attempts, which usually amount to beating Ash by proxy.
Both Yeager and Artax from Nodwick spend most of their time as dicks. Occasionally they show brief hints of the Jerk with a Heart of Goldwhen Piffany is in danger...but they spend the rest of their time planning horrible fates for Nodwick, as entertainment. This kind of treatment for a henchman is all but expected.
Si-Hoo from Orange Marmalade, who repeatedly likes to torment Ma-ri (having said multiple times that he would out her vampirism to the entire school). When Ma-ri had her pig-blood-bag stolen by Ah-ra, he gives her a real one and laughs over her while she's trying not to be sick. While he does force Ah-ra not to out Ma-ri as a vampire it's clear he does this to protect himself.
Mike from Shortpacked!, although he knows he's a jerk and makes a conscious effort to make everyone else miserable. He gets to keep his job at the store because he sells the most product insurance plans by outright threatening people.
The above notwithstanding, most readers actually like him, not just the author. Every evaluation above could be heavily questioned, though Squishydodo (the one whose son is now called "Squishybunbunbunbun") indeed seems to suffer from Stockholm Syndrome.
Most of the cast of Something*Positive. Davan likes to go up to random children and convince them their parents want to kill them, Jason enjoys trapping people into excuses to berate them, Aubrey and Peejee consider assaults on strangers and loved ones to be massively good fun. Kharisma, on the other hand, is currently serving time for a murder she didn't commit ... but was bragging that she was going to. And yet someone is trying to make a movie out of it. Mike started out this way, but he became more human and likeable throughout; at present, he's about the most sympathetic member of the main cast. Most of the cast claim to become sexually aroused by the suffering of others. At this point in his development, Mike is close to being the nicest guy in the strip, and there are times Kharisma comes off more sympathetic than the main cast; at least she's suffering for her sins. Mike seems to have realized what a bastard he was, and has decided that he needs to grow up and mature. He's the only person to actually make such a decision, with the rest of the main cast staying horrible, horrible people.
In Soul Symphony, Charlie's rants about how his bitter rival, Tom Mustaine, is one. He even wears sunglasses indoors.
Dillon, the title character of Sticky Dilly Buns, is probably meant to be cutely camp and whimsical, but shifts into jerkass territory with some readers for his casually manipulative attitude towards Ruby, his possessiveness towards Jerzy (which doesn't stop him from flirting with every other cute man in sight), and the tricks he uses to press his body up against clueless straight guy Andy. However, he improves significantly in volume 2, partly thanks to the influence of another character, Ruby, who manages to act as The Conscience (despite displaying some less attractive features of her own in the course of some Character Development).
Topato Potato from Wigu, who lives in a dimension whose survival depends on how much Magical Adventures in Space merchandise Wigu Tinkle purchases, and is willing to do anything, from beating his father to blowing up entire timelines to keep Wigu interested and buying. He would likely be even more of a Jerkass if his partner, Sheriff Pony, didn't serve as his conscience, if not a particularly successful one.
The Hat Guy from xkcd is a good example, asshownbythesestrips (given that he's an Expy of Aram). The Hat Guy's new girlfriend/archenemy also follows this trope.
As the Secretary arc proved, he has a long history of assholery. Proves he's something of a Jackass of all trades.
The Bird Feeder has Josh, a chickadee. He's not as horrible as some, but, as exemplified in #389, "Cruelty," when he kicks an innocent bug onto the ground in order to give an example of cruelty being humorous, he can definitely be a jerk sometimes.
Broheim: You know, if you really are stuck here, you might have a better time if you at least tried to assimilate. Gax: I learned your entire stupid language except for that word and I'm not gonna go back for it now.
Ziggy Pig in Ziggy Pig and Silly Seal (2022) is this, continuing the characterization established in the 2018 Ziggy Pig - Silly Seal Comics one-shot. He's essentially Eric Cartman in a cartoon pig skin.
where a friend comes to him for advice and is rewarded with smug mockery. That being said, Comic!Scott still manages to be somewhat sympathetic considering what he has to deal with.