- "Traction," where Bruce is critically injured after his first fight with Bane. Despite that, though, he's determined to get back out there in his new exoskeleton suit, but Alfred (who's pretty much raised Bruce since the deaths of the Waynes) is this close to walking out on Bruce rather than watch him risk his life like that again.Alfred: And you are doing what?Bruce: What you suggested, Alfred—getting on with my life.Alfred: Interesting interpretation, but know that if you intend to face this Bane again, sir, you may need to find another butler-slash-physician-slash-getaway driver!Bruce: ...I understand, Alfred.
- And the hardest part of this scene is, Bruce is quite prepared to continue the fight alone, injured as he is, while the closest thing he has to a second father figure is about to abandon him. Then Alfred looks back at one particular moment when Bruce, struggling to tighten a screw on the exoskeleton with a wrench, groans in pain from his injuries...one has to wonder, in that moment, if he can really leave his surrogate son to face such a deadly foe alone and in his condition. And then...
- Clayface's origin. He's essentially this show's Two-Face. The worst part was when he had gotten his form back to Ethan, and he got that look of unadulterated joy on his face... only to realize he can't maintain it.
- The ending to "Riddler's Revenge."
Riddler: I was ruined. In that moment, I lost my only chance to...
- Also the scene where it shows how Riddler lost his job at Gotham University. It's heart-breaking to know that Edward had a chance at a normal, possibly even successful, life. And then later reveal that Julie was the one who sabotaged his invention makes it even worse.
Batman: To what?
Riddler: To not be The Riddler.
Riddler: I thought you understood me!Julie: I understood that you were a psychopath. One that would ruin any chance of success!
- Riddlers reaction to finding out that Julie, the one person he trusted and even revealed his Dark and Troubled Past to, was the one who sabatoged him.
- The part in "A Matter of Family" where the Flying Graysons fall to their death. The audience is horrified, especially Bruce, whose reaction is gutwrenching, and then we see Dick crying; the funeral scene after that really cranks up the water works. Even Zucco's brothers were horrified at what he's done.
- One issue of The Batman Strikes has Poison Ivy escaping from prison to presumably steal a rare plant. At the end, when she's caught, she actually breaks into tears and says that she didn't want to steal it, she just wanted to see it since she's so deprived of plantlife at Arkham.
- The end of "Strange Minds," where we see The Joker of all people, curled up in a corner of his cell at Arkham, and crying.
Past!Joker: Let me guess, youre looking for the detective.
- And earlier in that same episode, the brief scene where while Batman is in Joker's mind, he comes across the man Joker once was before he fell into that tank of chemicals. And unlike his previous incarnations, where he was part of a gang, it's heavily implied that this version was just a plain office drone who dreamed of making people laugh, until that "one rotten day" transformed him into the crazy crime clown we know now, which makes what little we know of his backstory downright tragic.
Past!Joker: The only shadow of his former self left in this crazy place? Its my day job. What I really want is to make people laugh...
- In the midst of the Nightmare Fuel from "Strange New World," we see Dick practically having a breakdown after the whole city gets infected. It gets worse for him when he and Bruce discover Alfred is infected too. The way his voice wavers when he laments that they're all alone and they can't save the city, it almost sounds like the poor boy is ready to cry because he's so scared.
- The ending of "White Heat". Even after everything he tried to do, you can't help but feel bad for Firefly/Phosphorus. While the doctors at Arkham were able to surpress the fire powers caused by the radioactive isotope he had stolen, the physical and mental effects caused by it are irreversible. He's stuck like that. And to twist the knife even further, his girlfriend Blaze leaves him.
- In "Two of a Kind", that one moment where Joker snaps at Harley for asking him a simple favor, telling her he's doing what she wants only because it was fun. She wilts at his verbal abuse, before he changes his tune and "forgives" her. It's a sobering reminder that this Joker is just as abusive as his previous incarnations.
- Worse yet, the ending so painfully mirrors "Mad Love" from "Batman the Animated Series". Joker does something that ultimately shows how little he cares for her (throws her out a window/abandons her in a burning building). Batman sums up to Harley how Joker doesn't care about anyone but himself. A heartbroken Harley is faced with the sobering reality that he never loved her. But she only comes to her senses for a short while before "Mr. J" leaves behind a gift (A rose with a note to get well soon/A whoopee cushion telling her to keep smiling) that changes her mind. And thus, the cycle starts again and Harley Quinn is fated to remain the Joker's hopelessly lovesick, battered girlfriend.
- In "Fistful of Felt", the ending where the fight between Scarface and Mr. Snoots climaxes with both of them getting run over by a train, to the point where the two dummies are crippled beyond belief. What's heartbreaking was, Mr. Snoots was a better influence on Wesker. And the poor man is left weeping "He was the best, most wonderful friend in the world!" Batman can only hope that he's talking about Mr. Snoots, rather than his toxic friend Scarface.
Tear Jerker / The Batman